##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17534262
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72801883535218	33.0	33.0	33.0	33.0	33.0
2	32.773289403340726	33.0	33.0	33.0	33.0	33.0
3	33.45287511958017	33.0	33.0	33.0	33.0	37.0
4	36.56178412299303	37.0	37.0	37.0	37.0	37.0
5	36.584052297153995	37.0	37.0	37.0	37.0	37.0
6	36.54823710287892	37.0	37.0	37.0	37.0	37.0
7	36.48597597093051	37.0	37.0	37.0	37.0	37.0
8	36.508162761569324	37.0	37.0	37.0	37.0	37.0
9	36.56600722630927	37.0	37.0	37.0	37.0	37.0
10-11	36.56297236804149	37.0	37.0	37.0	37.0	37.0
12-13	36.7982454579497	37.0	37.0	37.0	37.0	38.5
14-15	38.58297166997961	40.0	37.0	40.0	37.0	40.0
16-17	38.519310108403765	40.0	37.0	40.0	37.0	40.0
18-19	38.565877480329654	40.0	37.0	40.0	37.0	40.0
20-21	38.58331195024333	40.0	37.0	40.0	37.0	40.0
22-23	38.57528484570904	40.0	37.0	40.0	37.0	40.0
24-25	38.57772919276172	40.0	37.0	40.0	37.0	40.0
26-27	38.488280021575825	40.0	37.0	40.0	37.0	40.0
28-29	38.37539881693803	40.0	37.0	40.0	37.0	40.0
30-31	38.21671603424032	40.0	37.0	40.0	37.0	40.0
32-33	38.12007747847469	40.0	37.0	40.0	37.0	40.0
34-35	38.099462493791826	40.0	37.0	40.0	37.0	40.0
36-37	38.07317712088392	40.0	37.0	40.0	37.0	40.0
38-39	38.04720269160654	40.0	37.0	40.0	37.0	40.0
40-41	38.026683483555175	40.0	37.0	40.0	35.0	40.0
42-43	37.9791290406736	40.0	37.0	40.0	33.0	40.0
44-45	37.87592147969803	40.0	37.0	40.0	33.0	40.0
46-47	37.791827073645166	40.0	37.0	40.0	33.0	40.0
48-49	37.74307722893605	40.0	37.0	40.0	33.0	40.0
50-51	37.71171013084411	40.0	37.0	40.0	33.0	40.0
52-53	37.654082551639405	40.0	37.0	40.0	33.0	40.0
54-55	37.562722798209506	40.0	37.0	40.0	33.0	40.0
56-57	37.39501291569117	40.0	37.0	40.0	33.0	40.0
58-59	37.19695826998502	37.0	37.0	40.0	33.0	40.0
60-61	37.05173507411224	37.0	37.0	40.0	33.0	40.0
62-63	36.92359639546896	37.0	37.0	40.0	33.0	40.0
64-65	36.76374441528697	37.0	37.0	40.0	33.0	40.0
66-67	36.57565696023374	37.0	37.0	40.0	33.0	40.0
68-69	36.38093937886493	37.0	37.0	40.0	33.0	40.0
70-71	36.16388861189579	37.0	37.0	38.5	33.0	40.0
72-73	35.85895388306735	37.0	37.0	37.0	33.0	40.0
74-75	35.25387831760922	37.0	33.0	37.0	30.0	40.0
76-77	33.662160614936724	35.0	33.0	37.0	27.0	37.0
78-79	35.106268695035936	37.0	33.0	37.0	33.0	37.0
80-81	35.27494495428902	37.0	33.0	37.0	33.0	37.0
82-83	35.13665880436832	37.0	33.0	37.0	33.0	37.0
84-85	34.97583333684007	37.0	33.0	37.0	33.0	37.0
86-87	34.86865765686534	37.0	33.0	37.0	33.0	37.0
88-89	34.7781239447657	37.0	33.0	37.0	33.0	37.0
90-91	34.69833225364303	37.0	33.0	37.0	33.0	37.0
92-93	34.68352816849848	37.0	33.0	37.0	33.0	37.0
94-95	34.660513929397425	37.0	33.0	37.0	33.0	37.0
96-97	34.66371114762117	37.0	33.0	37.0	33.0	37.0
98-99	34.6754582514032	37.0	33.0	37.0	33.0	37.0
100	34.60986210035955	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	6.0
18	11.0
19	42.0
20	134.0
21	542.0
22	2024.0
23	5645.0
24	13353.0
25	26856.0
26	47647.0
27	76832.0
28	115469.0
29	165158.0
30	227029.0
31	309291.0
32	416433.0
33	566835.0
34	792603.0
35	1164150.0
36	2072997.0
37	5056784.0
38	6203268.0
39	271148.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.96103053226145	0.022401883400706275	26.373809187053443	28.6427583972844
2	14.922183548617246	23.810049041274173	39.93630736714115	21.331460042967436
3	16.0858666307142	26.247355035529868	33.63507970851582	24.031698625240118
4	12.637999820009533	23.324705653422996	38.43406697128171	25.603227555285763
5	16.59444900472281	37.04714444481007	32.53555995316826	13.822846597298854
6	26.271530561137958	38.30300927407153	20.903292080385246	14.522168084405262
7	24.730504737582308	34.02712746360553	23.42630940798186	17.816058390830296
8	22.96720688713371	32.608554189994095	23.40699730658737	21.017241616284828
9	23.704510506043576	21.158628993407813	24.29139647390497	30.845464026643636
10-11	24.39133077714526	26.33375121545639	28.996575606042907	20.27834240135544
12-13	24.567529040142777	24.812402856367843	29.247088799403375	21.372979304086005
14-15	21.958540370846517	26.97809009583637	27.898522333018633	23.164847200298478
16-17	20.128631589969398	29.108983885378237	29.218629218612108	21.543755306040254
18-19	20.03033888823889	27.937714970202766	31.64605193095814	20.3858942106002
20-21	21.13383265735609	27.127277949482377	31.62662661078394	20.11226278237759
22-23	20.962440361873913	26.837861900364235	31.41304416336444	20.78665357439742
24-25	20.418256895696256	27.30111169664485	31.739317336632734	20.54131407102616
26-27	20.411162460205972	27.604937184932147	31.263390374838878	20.720509980023
28-29	20.347487776477767	27.515225179423116	31.398933938203083	20.73835310589604
30-31	20.700393493320174	27.47953889120584	31.264514127203075	20.55555348827091
32-33	20.419839423960763	27.88149878246429	31.05094623914482	20.64771555443013
34-35	20.474594942864897	27.802205364367843	31.077578141579526	20.645621551187734
36-37	20.696302171853223	27.81806850990617	30.910160608512133	20.575468709728472
38-39	20.448601949972936	28.117648597867024	30.816552706764966	20.61719674539508
40-41	20.797801213577245	27.859928919758364	30.61301996673692	20.729249899927467
42-43	20.86343818877919	27.839196154376754	30.327241289008978	20.970124367835076
44-45	20.82933343659756	28.546397624542735	29.67238302286392	20.951885915995785
46-47	21.099402670342144	28.28445652692238	29.175917929534663	21.440222873200813
48-49	21.145202188221816	28.462280081561914	29.02187756487385	21.37064016534242
50-51	21.023883101151007	28.676853239922863	28.677330705900246	21.621932953025887
52-53	21.20068963601055	28.48728565536827	28.594351060897438	21.717673647723736
54-55	21.12801798518114	28.635358027902473	28.56427322960393	21.672350757312458
56-57	21.16876659515137	28.842075722906163	28.400125454573498	21.589032227368964
58-59	21.33875118674856	28.69808102067795	28.526885859085997	21.43628193348749
60-61	21.280665945649883	28.62781627123068	28.553936120480405	21.537581662639028
62-63	21.307369109958714	28.71096424733206	28.37745982972182	21.60420681298741
64-65	21.216437722458796	28.514402605899058	28.43999618056375	21.8291634910784
66-67	21.366645086188228	28.633085924306766	28.24084022186532	21.75942876763968
68-69	21.352995888724994	28.62689589436169	28.367611188488272	21.652497028425042
70-71	21.445026784569965	28.11077924784877	28.403571029297893	22.04062293828337
72-73	21.38765279212273	28.431744033808236	28.445434963167614	21.735168210901417
74-75	21.327058051973495	28.492214808449308	28.47134075995406	21.709386379623133
76-77	21.52942560993776	28.253368343631557	28.154451273693148	22.062754772737534
78-79	21.39268716865626	28.261088035118142	28.386017153116622	21.96020764310898
80-81	21.341958387984413	28.390111895957844	28.407835235389005	21.86009448066874
82-83	21.768802741331946	27.840662546080768	28.540830597199374	21.84970411538791
84-85	21.797354469843707	27.97953608314075	28.433122618530053	21.78998682848549
86-87	21.56245005768951	28.32146650129259	28.23909827448189	21.876985166536013
88-89	21.809903405407933	27.85355673222183	28.28555959397292	22.050980268397318
90-91	21.929164102748288	27.75636875681594	28.344480334050857	21.96998680638491
92-93	21.771702465818837	27.890332398252593	28.402005299836965	21.935959836091605
94-95	21.76169654011827	27.623565727442383	28.464449595514147	22.150288136925194
96-97	21.72129298014199	28.060294067518647	28.56417734903897	21.65423560330039
98-99	22.924511902346755	30.050938368627516	27.905409526905373	19.11914020212036
100	22.295020752230812	29.838057109472654	22.344329234223874	25.52259290407266
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3052.0
1	3330.0
2	7476.0
3	12076.5
4	17857.0
5	33636.0
6	46166.5
7	51200.0
8	51567.0
9	46679.5
10	40809.5
11	35047.5
12	37387.0
13	49650.0
14	81287.5
15	132804.0
16	181050.0
17	211116.5
18	217133.0
19	202602.0
20	178952.0
21	152824.0
22	132258.0
23	124724.5
24	129754.0
25	146920.0
26	175541.0
27	208124.5
28	240658.0
29	283934.0
30	330215.0
31	372244.5
32	422631.0
33	473989.5
34	523112.5
35	570357.0
36	624571.5
37	687028.0
38	717925.0
39	720661.5
40	728251.5
41	731568.5
42	736897.5
43	757302.0
44	779108.0
45	801631.5
46	815560.0
47	818876.0
48	810403.5
49	778009.0
50	733458.5
51	685562.0
52	629012.0
53	574813.0
54	518856.5
55	449139.0
56	380500.0
57	314772.5
58	257212.0
59	211400.0
60	166930.0
61	126397.5
62	94142.0
63	69272.0
64	50009.5
65	35499.0
66	26341.5
67	20390.5
68	15672.0
69	11981.5
70	9157.5
71	6399.5
72	4357.0
73	3190.5
74	2534.0
75	1875.0
76	1257.0
77	862.5
78	582.0
79	367.5
80	226.0
81	151.5
82	108.5
83	74.0
84	54.0
85	39.5
86	27.0
87	19.5
88	13.0
89	9.0
90	5.5
91	2.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2546863962680608E-4
2	2.9656223911790527E-4
3	0.0
4	0.0
5	5.70311998303664E-6
6	0.0
7	1.140623996607328E-5
8	5.70311998303664E-6
9	1.1976551964376944E-4
10-11	0.0012005067564292127
12-13	0.005395151503952661
14-15	0.0
16-17	0.0
18-19	2.85155999151832E-6
20-21	5.70336604964706E-6
22-23	2.8520147716112277E-6
24-25	2.5674346700558326E-5
26-27	0.0
28-29	2.856680564608059E-6
30-31	1.4311968569772062E-5
32-33	2.383996387355065E-4
34-35	0.0052020267534782895
36-37	0.04647112709731305
38-39	0.12474086046380892
40-41	0.14097525759943638
42-43	0.04649549725769013
44-45	0.0012645058071746906
46-47	6.156990007666992E-6
48-49	6.24603006233007E-6
50-51	3.1620262403277178E-6
52-53	0.0
54-55	0.0
56-57	3.2677268545027952E-6
58-59	5.948403744202909E-5
60-61	8.992570966344684E-4
62-63	0.016860936601694798
64-65	0.08961269548988314
66-67	0.18534536232818444
68-69	0.1842784311157095
70-71	0.06809543459063891
72-73	0.0046691094707661685
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	413.0
20-21	1644.0
22-23	3553.0
24-25	7284.0
26-27	13215.0
28-29	27187.0
30-31	56085.0
32-33	72588.0
34-35	100461.0
36-37	141542.0
38-39	170508.0
40-41	177859.0
42-43	212654.0
44-45	246310.0
46-47	239125.0
48-49	205909.0
50-51	177237.0
52-53	167675.0
54-55	169243.0
56-57	170869.0
58-59	173449.0
60-61	170660.0
62-63	168424.0
64-65	177464.0
66-67	191727.0
68-69	182160.0
70-71	185460.0
72-73	180671.0
74-75	177213.0
76-77	171630.0
78-79	182026.0
80-81	187905.0
82-83	178840.0
84-85	195420.0
86-87	205631.0
88-89	221604.0
90-91	218686.0
92-93	218071.0
94-95	263019.0
96-97	768489.0
98-99	2832794.0
100-101	7921558.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.04332522993326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.81263669576683	65.1470892962847
2	7.582661405082352	11.380562518601156
3	2.3596612011443057	5.312304688497814
4	1.0981974095802485	3.296495414952033
5	0.635797004421066	2.385616069149369
6	0.3820001379643078	1.719993635468096
7	0.2666041615104875	1.4004803959919618
8	0.18539535735795434	1.1130147278666136
9	0.12958199347233856	0.8751837312079014
>10	0.5328571331422907	6.328067867958334
>50	0.011628030479388757	0.5749835803713506
>100	0.002746146885091832	0.3243046009265859
>500	1.9090251008878754E-4	0.10199969023978125
>1k	4.242068319936362E-5	0.0399037824842567
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	1.4257799957591599E-5	2.85155999151832E-6
20-21	0.0	0.0	0.0	4.562495986429312E-5	5.70311998303664E-6
22-23	0.0	0.0	0.0	7.1288999787958E-5	5.70311998303664E-6
24-25	0.0	0.0	0.0	1.0265615969465953E-4	5.70311998303664E-6
26-27	0.0	0.0	0.0	1.996091994062824E-4	5.70311998303664E-6
28-29	0.0	0.0	0.0	8.55467997455496E-4	5.70311998303664E-6
30-31	0.0	0.0	0.0	0.0026148805122222993	5.70311998303664E-6
32-33	0.0	0.0	0.0	0.006373236581043445	5.70311998303664E-6
34-35	0.0	0.0	0.0	0.011072607447065636	5.70311998303664E-6
36-37	0.0	0.0	0.0	0.01801330446642123	5.70311998303664E-6
38-39	0.0	0.0	0.0	0.028940482353919432	5.70311998303664E-6
40-41	0.0	0.0	0.0	0.039670902602002865	5.70311998303664E-6
42-43	0.0	0.0	0.0	0.04986237801168934	5.70311998303664E-6
44-45	0.0	0.0	0.0	0.06076674341925539	5.70311998303664E-6
46-47	0.0	0.0	0.0	0.0713831012676781	5.70311998303664E-6
48-49	0.0	0.0	0.0	0.08142344399781411	5.70311998303664E-6
50-51	0.0	0.0	0.0	0.09100753712930718	5.70311998303664E-6
52-53	0.0	0.0	0.0	0.09998709954259838	5.70311998303664E-6
54-55	0.0	0.0	0.0	0.10713025732135176	5.70311998303664E-6
56-57	0.0	0.0	0.0	0.11297880686395584	5.70311998303664E-6
58-59	0.0	0.0	0.0	0.11889009072637331	5.70311998303664E-6
60-61	0.0	5.70311998303664E-6	0.0	0.12465024190924032	5.70311998303664E-6
62-63	0.0	5.70311998303664E-6	0.0	0.13051019769181046	5.70311998303664E-6
64-65	0.0	5.70311998303664E-6	0.0	0.1365070283539735	5.70311998303664E-6
66-67	0.0	5.70311998303664E-6	0.0	0.14261792141579727	5.70311998303664E-6
68-69	0.0	5.70311998303664E-6	0.0	0.1487544785175447	5.70311998303664E-6
70-71	0.0	5.70311998303664E-6	0.0	0.15421236434131075	5.70311998303664E-6
72-73	0.0	5.70311998303664E-6	0.0	0.15870071976796057	5.70311998303664E-6
74-75	0.0	5.70311998303664E-6	0.0	0.16241059931692592	5.70311998303664E-6
76-77	0.0	5.70311998303664E-6	0.0	0.1656471199072992	5.70311998303664E-6
78-79	0.0	5.70311998303664E-6	0.0	0.16770594622117543	5.70311998303664E-6
80-81	0.0	5.70311998303664E-6	0.0	0.16849297677883449	5.70311998303664E-6
82-83	0.0	5.70311998303664E-6	0.0	0.16906328877713817	5.70311998303664E-6
84-85	0.0	5.70311998303664E-6	0.0	0.16931422605639176	5.70311998303664E-6
86-87	0.0	5.70311998303664E-6	0.0	0.169425436896061	5.70311998303664E-6
88	0.0	5.70311998303664E-6	0.0	0.16943399157603553	5.70311998303664E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	42480	0.0	44.816494	1
GTATCAA	75805	0.0	34.113148	1
ATCAACG	89715	0.0	28.278893	3
ACGCAGA	93495	0.0	27.170193	7
AACGCAG	93945	0.0	27.048258	6
TCAACGC	94065	0.0	27.027615	4
TATCAAC	94710	0.0	27.00697	2
CAACGCA	94300	0.0	26.92229	5
CGCAGAG	106105	0.0	23.971891	8
TGCGAGT	3260	0.0	22.714283	94
GCAGAGT	117720	0.0	21.478575	9
ACATGGG	71540	0.0	20.784676	3
GTACATG	75395	0.0	20.640806	1
GGTTCAC	7390	0.0	20.570314	6
GTGGTAT	20500	0.0	20.55352	1
TACATGG	75210	0.0	20.29606	2
GAGTACT	77355	0.0	19.771353	12-13
TGATCGC	2635	0.0	19.333628	9
ACGGTAC	2030	0.0	19.06979	3
CAGAGTA	115910	0.0	19.022127	10-11
>>END_MODULE