FastQCFastQC Report
Fri 27 May 2016
SRR2139126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9545194
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137350.051.9176251
GTATCAA246650.038.7738461
TCAACGC299850.031.2781724
ATCAACG302600.030.9939173
CAACGCA306700.030.5795885
TATCAAC314700.030.0120582
AACGCAG313900.029.8389476
ACGCAGA358750.026.0720447
CGCAGAG364300.025.7835318
GTGGTAT66050.024.5761591
TGGTATC63750.024.220742
GTACATG268350.022.6551481
GCAGAGT419950.022.3249029
TACATGG271650.021.521662
ACATGGG272550.020.7403053
GAGTACT284100.020.62605912-13
CAGAGTA409450.020.08761210-11
AGAGTAC384750.019.45615610-11
GTACTTT308350.018.87488614-15
CATGGGG199450.018.4607624
Produced by FastQC (version 0.11.5)