##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139126_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9545194
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69780708490576	33.0	33.0	33.0	33.0	33.0
2	32.70948447983351	33.0	33.0	33.0	33.0	33.0
3	32.68423449539108	33.0	33.0	33.0	33.0	33.0
4	36.6278789095329	37.0	37.0	37.0	37.0	37.0
5	36.65216390573099	37.0	37.0	37.0	37.0	37.0
6	36.624027756795726	37.0	37.0	37.0	37.0	37.0
7	36.53060587348984	37.0	37.0	37.0	37.0	37.0
8	36.56430503141162	37.0	37.0	37.0	37.0	37.0
9	36.61791483756119	37.0	37.0	37.0	37.0	37.0
10-11	36.59824561973282	37.0	37.0	37.0	37.0	37.0
12-13	36.58427382408362	37.0	37.0	37.0	37.0	37.0
14-15	38.741782880473664	40.0	37.0	40.0	37.0	40.0
16-17	38.68422255220795	40.0	37.0	40.0	37.0	40.0
18-19	38.702231877110094	40.0	37.0	40.0	37.0	40.0
20-21	38.70719829305231	40.0	37.0	40.0	37.0	40.0
22-23	38.689228485363884	40.0	37.0	40.0	37.0	40.0
24-25	38.70909943601815	40.0	37.0	40.0	37.0	40.0
26-27	38.63707958713669	40.0	37.0	40.0	37.0	40.0
28-29	38.532532342486896	40.0	37.0	40.0	37.0	40.0
30-31	38.40500551439706	40.0	37.0	40.0	37.0	40.0
32-33	38.31226804483173	40.0	37.0	40.0	37.0	40.0
34-35	38.278517517502316	40.0	37.0	40.0	37.0	40.0
36-37	38.253760939681946	40.0	37.0	40.0	37.0	40.0
38-39	38.211397720771316	40.0	37.0	40.0	37.0	40.0
40-41	38.17161305752011	40.0	37.0	40.0	37.0	40.0
42-43	38.1199647299398	40.0	37.0	40.0	37.0	40.0
44-45	38.02123052841334	40.0	37.0	40.0	33.0	40.0
46-47	37.935273130377816	40.0	37.0	40.0	33.0	40.0
48-49	37.86922448065503	40.0	37.0	40.0	33.0	40.0
50-51	37.819450875578795	40.0	37.0	40.0	33.0	40.0
52-53	37.74417061488734	40.0	37.0	40.0	33.0	40.0
54-55	37.64151562508239	40.0	37.0	40.0	33.0	40.0
56-57	37.47598248370962	40.0	37.0	40.0	33.0	40.0
58-59	37.26592356854957	38.5	37.0	40.0	33.0	40.0
60-61	37.096240307784264	37.0	37.0	40.0	33.0	40.0
62-63	36.94821755408525	37.0	37.0	40.0	33.0	40.0
64-65	36.77064682361306	37.0	37.0	40.0	33.0	40.0
66-67	36.560536346622456	37.0	37.0	40.0	33.0	40.0
68-69	36.356219832704504	37.0	37.0	40.0	33.0	40.0
70-71	36.1372422520443	37.0	37.0	38.5	33.0	40.0
72-73	35.84640114180084	37.0	37.0	37.0	33.0	40.0
74-75	35.50934975239019	37.0	33.0	37.0	33.0	40.0
76-77	33.3543686930848	35.0	33.0	37.0	27.0	37.0
78-79	35.03087434135989	37.0	33.0	37.0	33.0	37.0
80-81	35.249648801514724	37.0	33.0	37.0	33.0	37.0
82-83	35.12183330021753	37.0	33.0	37.0	33.0	37.0
84-85	34.95375016207204	37.0	33.0	37.0	33.0	37.0
86-87	34.83913464842172	37.0	33.0	37.0	33.0	37.0
88-89	34.740697963713565	37.0	33.0	37.0	33.0	37.0
90-91	34.65713804349184	37.0	33.0	37.0	33.0	37.0
92-93	34.63585869778866	37.0	33.0	37.0	33.0	37.0
94-95	34.59777379167919	37.0	33.0	37.0	33.0	37.0
96-97	34.600053441383146	37.0	33.0	37.0	33.0	37.0
98-99	34.61304301132209	37.0	33.0	37.0	33.0	37.0
100	34.56861929636654	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	9.0
20	32.0
21	248.0
22	1032.0
23	2899.0
24	7189.0
25	14752.0
26	26328.0
27	42774.0
28	63966.0
29	90826.0
30	123987.0
31	166132.0
32	223061.0
33	302119.0
34	422548.0
35	633984.0
36	1159106.0
37	2912950.0
38	3267972.0
39	83278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79042353670339	20.263674054188947	14.690869562211098	23.255032846896565
2	14.821200445293833	20.465894854752538	42.388381627627325	22.324523072326304
3	18.919112592158942	25.40886020755576	29.87743360690207	25.794593593383226
4	12.708657362019043	17.477350381773277	38.47975221876056	31.33424003744712
5	13.645369596469175	37.33533336252778	35.306783707067666	13.71251333393538
6	30.618906226526143	37.285926299664524	18.40019176142465	13.694975712384682
7	27.144759970305476	32.30330363112578	22.575329532328	17.97660686624075
8	24.258124036033212	37.383137524496625	20.595223103899198	17.763515335570972
9	25.390107314738703	17.27956498317373	21.271322510574432	36.05900519151313
10-11	24.55324113894385	26.840072606172278	28.356406375815936	20.250279879067936
12-13	25.193302955402192	24.19675398658615	28.928915735422933	21.681027322588726
14-15	22.16354638784712	25.678786622880583	26.710939557645453	25.446727431626847
16-17	21.640194007581197	28.79420784952092	27.46108146151875	22.10451668137913
18-19	21.60886934304321	27.73033214411357	29.53336516785306	21.12743334499016
20-21	22.281574201536404	26.904091230322496	29.660913025073338	21.153421543067765
22-23	22.044175850104995	26.76185492846212	29.5183270967189	21.67564212471399
24-25	21.78157574821404	26.963465662461562	29.606095695042967	21.648862894281436
26-27	21.763802850557113	27.10544080002235	29.542241685525667	21.588514663894873
28-29	21.64075476407658	27.223829598902782	29.551393768191144	21.5840218688295
30-31	21.95257114939022	27.05548645544899	29.403301000641502	21.588641394519286
32-33	21.72181744213794	27.23690199743291	29.37609261714065	21.665187943288505
34-35	21.829030813246487	27.28915696274437	29.347961504951947	21.53385071905719
36-37	21.865025611829346	27.22457265839417	29.2578287052049	21.65257302457158
38-39	21.745150953363037	27.36117464957884	29.301624888060623	21.592049508997498
40-41	22.024650494538612	27.251938570844757	29.087524043337016	21.635886891279615
42-43	21.92162954348979	27.38276913948004	28.823766435169002	21.871834881861165
44-45	21.99180960427747	27.485063137376763	28.65067665838874	21.872450599957027
46-47	22.098108172916334	27.553033865315236	28.297104968933205	22.051752992835226
48-49	22.03474936385282	27.69487997251619	28.223717202345643	22.04665346128534
50-51	22.039453566432034	27.79400210109936	28.123554896733605	22.042989435735006
52-53	22.266310315125338	27.703128365772784	27.93568443861029	22.094876880491583
54-55	22.222271482213994	27.612644602021685	27.97106030216626	22.194023613598056
56-57	22.186139126614428	27.766709321047344	27.909181479191442	22.137970073146782
58-59	22.19330520411841	27.778517878101	27.907674747033496	22.120502170747095
60-61	22.233465909674354	27.7191735029378	27.97199272758962	22.07536785979823
62-63	22.193085203123562	27.82992088862166	27.900337108304196	22.076656799950577
64-65	22.18301062678294	27.81278826457369	27.836523405117624	22.16767770352574
66-67	22.260127255668156	27.81235649480111	27.687677196185163	22.239839053345573
68-69	22.20865283262419	27.787848437534603	27.760526382477725	22.242972347363487
70-71	22.221796467894865	27.618029583823482	27.775144420240277	22.38502952804137
72-73	22.296937601210878	27.617305146666965	27.70828758524037	22.377469666881787
74-75	22.13315989774229	27.804126218201908	27.690373194507185	22.372340689548622
76-77	22.353022935692042	27.714846540323673	27.55250683889302	22.379623685091264
78-79	22.325953638083075	27.687625467805223	27.561015232363506	22.425405661748197
80-81	22.291350379235134	27.712259795309063	27.571564263608987	22.424825561846816
82-83	22.3722673571511	27.681531593512602	27.604168011765555	22.342033037570737
84-85	22.474713189563246	27.69872440443702	27.51367583676104	22.312886569238692
86-87	22.297171749470305	27.719690670916382	27.600320392103566	22.382817187509747
88-89	22.35809659796712	27.71676992899484	27.54762497469222	22.377508498345815
90-91	22.395976722670447	27.73891771579876	27.524752834151933	22.34035272737886
92-93	22.466034335578108	27.705434885706154	27.50819817256315	22.320332606152583
94-95	22.356581571628915	27.759927770423126	27.52813931117532	22.355351346772643
96-97	22.429040038906315	27.83875421457877	27.623196258204764	22.109009488310154
98-99	23.556989352221557	29.747639767778672	27.788902427945178	18.906468452054597
100	22.54356342984838	30.634526812963404	21.63187760132182	25.190032155866398
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2848.0
1	2418.0
2	2932.5
3	4150.5
4	5464.0
5	8771.5
6	11864.0
7	14354.5
8	16335.5
9	16627.5
10	15395.5
11	13607.5
12	14171.5
13	18287.5
14	28674.0
15	43626.0
16	56388.0
17	64084.0
18	65959.0
19	62397.5
20	55622.5
21	47575.0
22	40809.0
23	38775.5
24	42131.5
25	50644.5
26	63322.0
27	78041.0
28	94051.5
29	113285.5
30	133959.0
31	155107.5
32	178808.5
33	202410.5
34	225739.5
35	247532.5
36	271971.0
37	295878.5
38	313182.5
39	327222.0
40	342791.0
41	357307.5
42	365831.0
43	375861.0
44	385815.0
45	393908.5
46	398189.5
47	399003.0
48	399438.5
49	391872.5
50	378363.5
51	363501.5
52	343717.5
53	321185.5
54	298614.0
55	272200.5
56	243462.0
57	214992.0
58	187934.0
59	161979.5
60	133497.0
61	106716.0
62	83797.5
63	63455.5
64	46597.5
65	33982.5
66	25262.5
67	19034.0
68	14329.5
69	10251.5
70	7138.5
71	4974.5
72	3384.5
73	2267.0
74	1544.5
75	1016.0
76	660.0
77	450.5
78	316.0
79	243.5
80	173.0
81	103.0
82	72.0
83	52.5
84	38.0
85	29.0
86	23.0
87	16.5
88	10.5
89	6.0
90	3.0
91	2.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.771531516279292E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.006867330302558545
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.667836531861448E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0018060748459531365
36-37	0.03685228597901662
38-39	0.11631275704140555
40-41	0.15067861349996547
42-43	0.05370887616266936
44-45	0.001476650622536642
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.008665946457508403
64-65	0.06962922968385096
66-67	0.16919306873129958
68-69	0.18726163962032677
70-71	0.07205117188926198
72-73	0.005025410999392903
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	147.0
20-21	576.0
22-23	1374.0
24-25	2802.0
26-27	3887.0
28-29	6373.0
30-31	13547.0
32-33	15847.0
34-35	21023.0
36-37	22759.0
38-39	24944.0
40-41	33849.0
42-43	40675.0
44-45	49319.0
46-47	51253.0
48-49	42191.0
50-51	35954.0
52-53	32586.0
54-55	43787.0
56-57	40889.0
58-59	42681.0
60-61	46500.0
62-63	49716.0
64-65	58814.0
66-67	69616.0
68-69	67716.0
70-71	64912.0
72-73	62686.0
74-75	65559.0
76-77	59295.0
78-79	64222.0
80-81	67509.0
82-83	70632.0
84-85	76682.0
86-87	82652.0
88-89	89121.0
90-91	91802.0
92-93	99679.0
94-95	121675.0
96-97	334819.0
98-99	630476.0
100-101	6744648.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.70614633475701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.74691776633631	71.69462503440164
2	8.190197176615435	13.38378898046109
3	2.1594417409916917	5.2931898867254885
4	0.8449182245998893	2.7614004840024657
5	0.41232592803773477	1.6844781306932823
6	0.21174758998990934	1.0380647744248597
7	0.12456726243240486	0.7124537680975506
8	0.0828279151625347	0.5414039805498319
9	0.04873572519001948	0.35838074646956114
>10	0.17373563051268945	2.170834538029181
>50	0.003542120795615421	0.19023119145100906
>100	9.713541507023151E-4	0.13117243786837654
>500	7.15651850859758E-5	0.039976046825628726
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0952952868218288E-5
2	0.0	4.1905905736436575E-5	0.0	0.0	2.0952952868218288E-5
3	0.0	5.238238217054572E-5	0.0	1.0476476434109144E-5	2.0952952868218288E-5
4	0.0	1.1524124077520059E-4	0.0	1.0476476434109144E-5	3.142942930232743E-5
5	0.0	1.2571771720930973E-4	0.0	1.0476476434109144E-5	3.142942930232743E-5
6	2.0952952868218288E-5	1.2571771720930973E-4	0.0	1.0476476434109144E-5	3.142942930232743E-5
7	2.0952952868218288E-5	1.3619419364341886E-4	0.0	1.0476476434109144E-5	3.142942930232743E-5
8	2.0952952868218288E-5	1.5714714651163716E-4	0.0	1.0476476434109144E-5	5.238238217054572E-5
9	2.0952952868218288E-5	2.619119108527286E-4	0.0	2.0952952868218288E-5	6.285885860465486E-5
10-11	2.0952952868218288E-5	2.619119108527286E-4	0.0	5.7620620387600295E-5	6.809709682170943E-5
12-13	2.0952952868218288E-5	2.619119108527286E-4	0.0	9.952652612403687E-5	8.905004968992773E-5
14-15	2.0952952868218288E-5	3.981061044961475E-4	0.0	1.0476476434109144E-4	1.2571771720930973E-4
16-17	3.142942930232743E-5	4.0858258093025664E-4	0.0	1.0476476434109144E-4	1.5714714651163716E-4
18-19	3.142942930232743E-5	4.295355337984749E-4	0.0	1.2571771720930973E-4	1.885765758139646E-4
20-21	3.142942930232743E-5	4.6096496310080236E-4	0.0	1.6238538472869175E-4	2.2000600511629201E-4
22-23	3.142942930232743E-5	4.766796777519661E-4	0.0	1.7810009937985547E-4	3.0381781658916516E-4
24-25	3.142942930232743E-5	5.238238217054572E-4	0.0	1.885765758139646E-4	3.5620019875971093E-4
26-27	3.142942930232743E-5	5.552532510077847E-4	0.0	2.5667367263567406E-4	3.614384369767655E-4
28-29	3.142942930232743E-5	5.552532510077847E-4	0.0	4.347737720155295E-4	3.6667667519382004E-4
30-31	3.142942930232743E-5	6.443033006977123E-4	0.0	0.0010005034994574231	3.6667667519382004E-4
32-33	3.142942930232743E-5	6.49541538914767E-4	0.0	0.0022576806715505207	4.0858258093025664E-4
34-35	3.666766751938201E-5	7.22876873953531E-4	0.0	0.0036039078933335455	4.6096496310080236E-4
36-37	4.1905905736436575E-5	7.857357325581858E-4	0.0	0.005667773750853047	4.7144143953491147E-4
38-39	4.1905905736436575E-5	8.171651618605132E-4	0.0	0.010654576533489	4.8191791596902063E-4
40-41	4.1905905736436575E-5	8.800240204651682E-4	0.0	0.01571995288938077	4.8191791596902063E-4
42-43	4.1905905736436575E-5	9.219299262016047E-4	0.0	0.01932909902093137	4.8191791596902063E-4
44-45	4.7144143953491144E-5	0.0010371711669768054	0.0	0.023147774681164156	4.976326306201843E-4
46-47	5.238238217054572E-5	0.0011000300255814602	0.0	0.027191694584730284	5.290620599225118E-4
48-49	5.238238217054572E-5	0.0011105065020155693	0.0	0.03220468855845151	5.657297274418938E-4
50-51	5.7620620387600295E-5	0.0012938448396124793	0.0	0.0371757766264363	5.657297274418938E-4
52-53	6.809709682170943E-5	0.001309559554263643	0.0	0.052020943733568956	5.814444420930574E-4
54-55	7.333533503876401E-5	0.0013252742689148066	0.0	0.07239245215969418	6.12873871395385E-4
56-57	7.333533503876401E-5	0.001372418412868298	0.0	0.09576547108419169	6.338268242636032E-4
58-59	7.333533503876401E-5	0.001372418412868298	0.0	0.11076778533783599	6.390650624806578E-4
60-61	8.905004968992773E-5	0.0013986096039535706	0.0	0.1225381066115576	6.390650624806578E-4
62-63	1.1524124077520059E-4	0.0014509919861241164	0.0	0.1334965009616358	6.547797771318215E-4
64-65	1.1524124077520059E-4	0.001665759753023354	0.0	0.13800662406651976	6.704944917829852E-4
66-67	1.2571771720930973E-4	0.001676236229457463	0.0	0.1432029563778379	7.071621593023672E-4
68-69	1.2571771720930973E-4	0.0017338568498450634	0.0	0.14861929469427232	7.385915886046947E-4
70-71	1.2571771720930973E-4	0.0017705245173644454	0.0	0.15266321459783846	7.857357325581858E-4
72-73	1.4667067007752803E-4	0.0017810009937985545	0.0	0.15563853390512544	9.114534497674956E-4
74-75	1.4667067007752803E-4	0.001801953946666773	0.0	0.15811622058179226	9.114534497674956E-4
76-77	1.4667067007752803E-4	0.001875289281705537	0.0	0.16036866301512573	9.114534497674956E-4
78-79	1.4667067007752803E-4	0.0019171951874419734	0.0	0.16189822857450564	9.324064026357138E-4
80-81	1.4667067007752803E-4	0.0019171951874419734	0.0	0.16246395830194754	9.585975937209867E-4
82-83	1.5190890829458258E-4	0.0019276716638760825	0.0	0.16300349683830417	0.0010057417376744778
84-85	1.5714714651163716E-4	0.0019486246167443007	0.0	0.1631973116523352	0.001016218214108587
86-87	1.5714714651163716E-4	0.001980054046046628	0.0	0.1632654087491569	0.001016218214108587
88	1.5714714651163716E-4	0.002032436428217174	0.0	0.16327588522559103	0.001016218214108587
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13735	0.0	51.917625	1
GTATCAA	24665	0.0	38.773846	1
TCAACGC	29985	0.0	31.278172	4
ATCAACG	30260	0.0	30.993917	3
CAACGCA	30670	0.0	30.579588	5
TATCAAC	31470	0.0	30.012058	2
AACGCAG	31390	0.0	29.838947	6
ACGCAGA	35875	0.0	26.072044	7
CGCAGAG	36430	0.0	25.783531	8
GTGGTAT	6605	0.0	24.576159	1
TGGTATC	6375	0.0	24.22074	2
GTACATG	26835	0.0	22.655148	1
GCAGAGT	41995	0.0	22.324902	9
TACATGG	27165	0.0	21.52166	2
ACATGGG	27255	0.0	20.740305	3
GAGTACT	28410	0.0	20.626059	12-13
CAGAGTA	40945	0.0	20.087612	10-11
AGAGTAC	38475	0.0	19.456156	10-11
GTACTTT	30835	0.0	18.874886	14-15
CATGGGG	19945	0.0	18.460762	4
>>END_MODULE