FastQCFastQC Report
Fri 27 May 2016
SRR2139125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8014878
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175750.046.9640241
CTTATAC127250.038.1095471
GTATCAA323750.036.557321
TATACAC146550.034.635453
TTATACA162850.030.46112
ATCAACG407600.028.7337073
TATCAAC412050.028.5334682
TCAACGC414600.028.3577944
CAACGCA415450.028.2304135
AACGCAG417900.028.1071176
ACGCAGA466600.025.129567
CGCAGAG469600.025.004098
TACACAT206650.024.5425035
ACACATC207300.023.8721796
ATACACA220350.023.8386234
GCAGAGT527050.022.1379629
CACATCT227050.021.6688587
GAGTACT331450.020.49433712-13
GTACATG347450.019.7036271
TACATGG344650.019.6367572
Produced by FastQC (version 0.11.5)