##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139125_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8014878
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6513784738832	33.0	33.0	33.0	33.0	33.0
2	32.665629470592066	33.0	33.0	33.0	33.0	33.0
3	32.63484110425636	33.0	33.0	33.0	33.0	33.0
4	36.572672098065624	37.0	37.0	37.0	37.0	37.0
5	36.60167366240634	37.0	37.0	37.0	37.0	37.0
6	36.57560464426283	37.0	37.0	37.0	37.0	37.0
7	36.48030899534591	37.0	37.0	37.0	37.0	37.0
8	36.5165106692828	37.0	37.0	37.0	37.0	37.0
9	36.57072958565308	37.0	37.0	37.0	37.0	37.0
10-11	36.55079141566472	37.0	37.0	37.0	37.0	37.0
12-13	36.53659088759679	37.0	37.0	37.0	37.0	37.0
14-15	38.60736626309222	40.0	37.0	40.0	37.0	40.0
16-17	38.541510925057125	40.0	37.0	40.0	37.0	40.0
18-19	38.58174959119777	40.0	37.0	40.0	37.0	40.0
20-21	38.6021495620388	40.0	37.0	40.0	37.0	40.0
22-23	38.58350260267238	40.0	37.0	40.0	37.0	40.0
24-25	38.597894256764235	40.0	37.0	40.0	37.0	40.0
26-27	38.51233126153483	40.0	37.0	40.0	37.0	40.0
28-29	38.396128929426	40.0	37.0	40.0	37.0	40.0
30-31	38.24563226689171	40.0	37.0	40.0	37.0	40.0
32-33	38.13452996868619	40.0	37.0	40.0	37.0	40.0
34-35	38.08887342775787	40.0	37.0	40.0	37.0	40.0
36-37	38.064957479979455	40.0	37.0	40.0	37.0	40.0
38-39	38.01445823372981	40.0	37.0	40.0	33.0	40.0
40-41	37.994336920110214	40.0	37.0	40.0	33.0	40.0
42-43	37.94962112505698	40.0	37.0	40.0	33.0	40.0
44-45	37.842422716602066	40.0	37.0	40.0	33.0	40.0
46-47	37.75355468593095	40.0	37.0	40.0	33.0	40.0
48-49	37.6994549535263	40.0	37.0	40.0	33.0	40.0
50-51	37.63072362674025	40.0	37.0	40.0	33.0	40.0
52-53	37.57710726692201	40.0	37.0	40.0	33.0	40.0
54-55	37.46668256002022	40.0	37.0	40.0	33.0	40.0
56-57	37.27574791080055	38.5	37.0	40.0	33.0	40.0
58-59	37.06384285221687	37.0	37.0	40.0	33.0	40.0
60-61	36.885940597661346	37.0	37.0	40.0	33.0	40.0
62-63	36.75201325661301	37.0	37.0	40.0	33.0	40.0
64-65	36.603985809013764	37.0	37.0	40.0	33.0	40.0
66-67	36.40540702926503	37.0	37.0	40.0	33.0	40.0
68-69	36.219523135415415	37.0	37.0	40.0	33.0	40.0
70-71	36.021957818143235	37.0	37.0	37.0	33.0	40.0
72-73	35.74744317110516	37.0	35.0	37.0	33.0	40.0
74-75	35.41878715286665	37.0	33.0	37.0	33.0	40.0
76-77	33.21970268932654	35.0	33.0	37.0	27.0	37.0
78-79	34.93945167803662	37.0	33.0	37.0	33.0	37.0
80-81	35.1858983776047	37.0	33.0	37.0	33.0	37.0
82-83	35.07233414304582	37.0	33.0	37.0	33.0	37.0
84-85	34.91881174804113	37.0	33.0	37.0	33.0	37.0
86-87	34.81739721199724	37.0	33.0	37.0	33.0	37.0
88-89	34.730534684039824	37.0	33.0	37.0	33.0	37.0
90-91	34.65576793459908	37.0	33.0	37.0	33.0	37.0
92-93	34.64419950772198	37.0	33.0	37.0	33.0	37.0
94-95	34.61411233981396	37.0	33.0	37.0	33.0	37.0
96-97	34.62753021073915	37.0	33.0	37.0	33.0	37.0
98-99	34.647882010238774	37.0	33.0	37.0	33.0	37.0
100	34.61004818822111	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	4.0
19	9.0
20	42.0
21	273.0
22	1005.0
23	2968.0
24	6884.0
25	13673.0
26	24522.0
27	39158.0
28	57779.0
29	81052.0
30	109732.0
31	146806.0
32	195104.0
33	263287.0
34	366301.0
35	542337.0
36	986693.0
37	2445936.0
38	2640810.0
39	90502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31749511782839	20.36856215866596	14.81577506626428	23.49816765724136
2	14.335570061160352	20.40101147459966	42.932098522826365	22.33131994141363
3	17.90529037438753	26.784478414328756	30.909257871331025	24.400973339952692
4	11.960982058739017	18.249812193000594	38.4014377263681	31.38776802189229
5	12.827096410836997	37.900357048523645	35.42454862376553	13.847997916873833
6	28.999110030010446	38.06070885429683	18.599412068332427	14.340769047360302
7	25.84331289883639	33.30488623781921	22.53525256404402	18.316548299300376
8	24.397514222923917	36.264931294025935	21.17226238502944	18.165292098020704
9	25.436768968910066	16.5819616967345	21.172511920955003	36.80875741340043
10-11	24.125782076782702	26.724598927145244	28.373750667196678	20.775868328875376
12-13	25.213363753709743	24.2710620242527	28.62752070812847	21.888053513909085
14-15	22.366703523122872	25.593714589292567	26.840695516513165	25.1988863710714
16-17	21.244403221109543	28.888811532751962	27.421502860056012	22.445282386082482
18-19	21.007631058139626	27.188461259173252	30.12687155063371	21.677036132053416
20-21	21.933500545631816	26.219342990920776	30.254934705103036	21.592221758344373
22-23	21.657626620338768	26.5824157476251	30.06364678455456	21.69631084748157
24-25	21.261359674868878	26.933667574371402	30.437419485774893	21.367553264984835
26-27	21.129863157586513	27.001535927025706	30.4575871305036	21.411013784884183
28-29	20.652804077490362	26.709358787824332	30.490962933842685	22.146874200842618
30-31	21.099068689085822	26.535403591953333	30.56336413916852	21.802163579792325
32-33	20.814686524500253	26.99392697932058	30.451906586882	21.739479909297167
34-35	20.982606545838458	27.452485738013245	30.53506228981078	21.029845426337516
36-37	21.30201459686973	27.06123049859942	29.841431390608502	21.795323513922348
38-39	21.343154253306025	26.842495469361253	30.074070738537127	21.74027953879559
40-41	21.435772608493313	26.92746794700267	30.089699394797474	21.54706004970654
42-43	21.73109500635124	26.902315321772154	29.771716885243414	21.594872786633196
44-45	21.505272440689946	27.5071851377807	29.192274043129085	21.795268378400273
46-47	22.048348855452062	27.098300008502097	28.372364550642814	22.480986585403024
48-49	22.053050022814784	27.220506730597716	28.32311961568774	22.40332363089976
50-51	21.690678823118382	27.54893679334135	28.34326907923138	22.417115304308886
52-53	21.842906957993478	27.741775592906613	28.38353616659111	22.031781282508803
54-55	21.87396731050414	27.728914496136873	27.962854660563035	22.43426353279595
56-57	22.340650447839607	28.011202345544064	27.424618608999722	22.223528597616607
58-59	21.720933174975848	28.32692790886272	28.104939116598267	21.84719979956317
60-61	22.16093953615911	28.683923752666256	27.263837937280382	21.891298773894253
62-63	21.84986203978739	28.835876841088755	27.17379173910525	22.140469380018608
64-65	21.880873962764536	28.609525143487975	27.272970584082902	22.236630309664594
66-67	22.12711689433699	28.31488604245268	27.232125218414005	22.325871844796325
68-69	22.147960530936377	27.99160823890481	27.411404538887112	22.449026691271705
70-71	22.128202971174083	27.55617617082875	27.521231671486042	22.79438918651113
72-73	22.272626555549742	27.612588627198658	27.565679637377134	22.549105179874466
74-75	22.286023187430075	27.677918477668978	27.429601100957395	22.606457233943548
76-77	22.23262326969275	27.59684696676073	27.323744745378274	22.84678501816825
78-79	22.31837611505906	27.614762013653323	27.2900656189833	22.77679625230432
80-81	22.299052880232978	27.537124382426626	27.386892494945275	22.776930242395117
82-83	22.47551207597994	27.324600033612988	27.597042587391286	22.602845303015787
84-85	22.77980577672098	27.315287286850893	27.273454735617776	22.631452200810354
86-87	22.30371078922725	27.497277808778914	27.399196324507503	22.799815077486333
88-89	22.59355363089246	27.263568828755076	27.40367196402297	22.739205576329503
90-91	22.652228924773624	27.10713618838485	27.498872710134826	22.741762176706704
92-93	22.69817066232941	27.167511209983992	27.41538154281613	22.71893658487047
94-95	22.39389363103916	27.18785706866106	27.62153318790901	22.796716112390776
96-97	22.5445273873972	27.289373805502244	27.667391140351523	22.49870766674903
98-99	23.66152156479478	29.223531513093782	27.802409299486374	19.31253762262506
100	22.7155895796253	29.99253171480973	21.44032907872521	25.851549626839766
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1720.0
1	1825.5
2	3572.5
3	5529.0
4	8070.0
5	14659.0
6	19942.5
7	22566.0
8	23501.0
9	21945.5
10	19359.0
11	16976.0
12	18389.0
13	25079.0
14	41079.0
15	63762.5
16	82298.0
17	92653.0
18	94175.5
19	87364.5
20	76204.5
21	64091.0
22	54538.0
23	50991.5
24	53767.0
25	62358.5
26	75234.5
27	88956.5
28	100191.0
29	113359.5
30	127107.0
31	139912.5
32	156909.5
33	173421.5
34	190906.0
35	210747.5
36	232726.0
37	254418.0
38	263467.0
39	266473.0
40	276784.5
41	287497.5
42	294558.0
43	306924.0
44	326342.0
45	351486.0
46	375002.5
47	389905.5
48	388113.0
49	376782.5
50	368269.5
51	350819.0
52	327014.0
53	307231.0
54	285538.0
55	257907.5
56	229508.0
57	199061.5
58	169426.5
59	142434.0
60	114329.0
61	88098.0
62	65308.0
63	47317.0
64	34096.5
65	23583.0
66	16506.0
67	12238.5
68	8845.5
69	6115.0
70	4376.0
71	3250.5
72	2355.5
73	1610.5
74	1136.0
75	776.5
76	532.5
77	365.0
78	246.5
79	158.0
80	113.5
81	85.0
82	65.0
83	44.5
84	30.5
85	24.5
86	20.5
87	17.5
88	12.5
89	7.5
90	3.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4953592556243527E-5
2	5.614558325154794E-4
3	1.2476796278121763E-5
4	1.2476796278121763E-5
5	1.2476796278121763E-5
6	1.2476796278121763E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0064005964906764645
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.744483439772356E-5
26-27	0.0
28-29	6.247992832302622E-6
30-31	6.2595246491943304E-6
32-33	0.0
34-35	0.001582590944755092
36-37	0.035443516001859805
38-39	0.1153450804211533
40-41	0.15174623224006045
42-43	0.053267178388342995
44-45	0.0015160026937390473
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.009010779869433874
64-65	0.07262702020830493
66-67	0.18086271302967893
68-69	0.2017189496902441
70-71	0.07740409399557749
72-73	0.005515635101877854
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	185.0
20-21	762.0
22-23	1597.0
24-25	2777.0
26-27	4698.0
28-29	11273.0
30-31	24203.0
32-33	30195.0
34-35	40720.0
36-37	55995.0
38-39	67642.0
40-41	74111.0
42-43	89013.0
44-45	104937.0
46-47	100869.0
48-49	86788.0
50-51	72049.0
52-53	65864.0
54-55	78038.0
56-57	86524.0
58-59	86885.0
60-61	88206.0
62-63	88689.0
64-65	93262.0
66-67	102941.0
68-69	83600.0
70-71	74215.0
72-73	68980.0
74-75	68641.0
76-77	65029.0
78-79	69050.0
80-81	74631.0
82-83	77223.0
84-85	82648.0
86-87	85465.0
88-89	91315.0
90-91	90345.0
92-93	94375.0
94-95	104097.0
96-97	247271.0
98-99	455794.0
100-101	4723976.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.50758995825417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3261952961951	69.43095328224305
2	7.893408351934347	12.551717492373099
3	2.3495750848608856	5.6042715726974865
4	1.0206515277822996	3.24598172644723
5	0.5086573026614883	2.02210581246406
6	0.2904581898038926	1.3856178392966796
7	0.16606688806339942	0.9242504629251639
8	0.11273118744382557	0.717038802143262
9	0.07671402748395877	0.548941269711675
>10	0.25114495852161345	2.8936930765513953
>50	0.0029414566456787907	0.15934691873794712
>100	0.0010415896300386446	0.17353765660975204
>500	1.911410920189634E-4	0.09769245464628704
>1k	2.2299788150221796E-4	0.24485163315292965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.9907185112487054E-5
2	0.0	0.0	0.0	0.0	4.9907185112487054E-5
3	0.0	0.0	0.0	0.0	4.9907185112487054E-5
4	0.0	0.0	0.0	0.0	4.9907185112487054E-5
5	0.0	0.0	0.0	0.0	4.9907185112487054E-5
6	0.0	0.0	0.0	0.0	6.238398139060881E-5
7	0.0	0.0	0.0	0.0	7.486077766873058E-5
8	0.0	0.0	0.0	0.0	7.486077766873058E-5
9	0.0	0.0	0.0	0.0	8.733757394685235E-5
10-11	0.0	0.0	0.0	2.4953592556243527E-5	8.733757394685235E-5
12-13	0.0	0.0	0.0	4.366878697342617E-5	9.357597208591323E-5
14-15	0.0	0.0	0.0	6.86223795296697E-5	1.2476796278121762E-4
16-17	0.0	0.0	0.0	1.1852956464215674E-4	1.372447590593394E-4
18-19	0.0	0.0	0.0	1.684367497546438E-4	1.372447590593394E-4
20-21	0.0	0.0	0.0	2.495359255624353E-4	1.372447590593394E-4
22-23	0.0	0.0	0.0	6.987005915748187E-4	1.5595995347652205E-4
24-25	0.0	0.0	0.0	0.002220869737505674	1.746751478937047E-4
26-27	0.0	0.0	0.0	0.0056207967232938544	1.746751478937047E-4
28-29	0.0	0.0	0.0	0.016949727743828413	1.746751478937047E-4
30-31	0.0	0.0	0.0	0.019844344480352664	1.746751478937047E-4
32-33	0.0	0.0	0.0	0.02532165804644812	1.9962874044994822E-4
34-35	1.2476796278121763E-5	0.0	0.0	0.03204041284221669	1.9962874044994822E-4
36-37	1.2476796278121763E-5	0.0	0.0	0.04173488355031729	1.9962874044994822E-4
38-39	1.2476796278121763E-5	0.0	0.0	0.0654158428861924	1.9962874044994822E-4
40-41	1.2476796278121763E-5	0.0	0.0	0.09074997772891864	1.9962874044994822E-4
42-43	1.2476796278121763E-5	0.0	0.0	0.10753750712113147	2.058671385890091E-4
44-45	1.2476796278121763E-5	0.0	0.0	0.1282677041372308	2.1834393486713086E-4
46-47	1.2476796278121763E-5	0.0	0.0	0.15002723684627514	2.370591292843135E-4
48-49	1.2476796278121763E-5	0.0	0.0	0.16994644210429655	2.370591292843135E-4
50-51	1.2476796278121763E-5	0.0	0.0	0.1887988812805385	2.370591292843135E-4
52-53	1.2476796278121763E-5	0.0	0.0	0.22888058932400468	2.6825111997961793E-4
54-55	1.2476796278121763E-5	6.238398139060882E-6	0.0	0.2728800113988011	2.9944311067492234E-4
56-57	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.31519506597605107	2.9944311067492234E-4
58-59	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.3498181756478389	2.9944311067492234E-4
60-61	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.3649026223480881	3.119199069530441E-4
62-63	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.3830002153495038	3.119199069530441E-4
64-65	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.3891637527108959	3.119199069530441E-4
66-67	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.394809503026746	3.119199069530441E-4
68-69	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.4004614917407352	3.119199069530441E-4
70-71	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.40519643592828236	3.2439670323116586E-4
72-73	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.40934497069075787	4.117342771780182E-4
74-75	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.4128072816579367	4.117342771780182E-4
76-77	1.2476796278121763E-5	1.2476796278121763E-5	0.0	0.41608867907908265	4.117342771780182E-4
78-79	1.2476796278121763E-5	1.8715194417182646E-5	0.0	0.4181036816779994	4.117342771780182E-4
80-81	1.2476796278121763E-5	2.4953592556243527E-5	0.0	0.41885852785282573	4.117342771780182E-4
82-83	1.2476796278121763E-5	2.4953592556243527E-5	0.0	0.4195946588332349	4.117342771780182E-4
84-85	1.2476796278121763E-5	2.4953592556243527E-5	0.0	0.4199440091290223	4.117342771780182E-4
86-87	1.2476796278121763E-5	2.4953592556243527E-5	0.0	0.42009373068435973	4.117342771780182E-4
88	1.2476796278121763E-5	2.4953592556243527E-5	0.0	0.42010620748063787	4.117342771780182E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17575	0.0	46.964024	1
CTTATAC	12725	0.0	38.109547	1
GTATCAA	32375	0.0	36.55732	1
TATACAC	14655	0.0	34.63545	3
TTATACA	16285	0.0	30.4611	2
ATCAACG	40760	0.0	28.733707	3
TATCAAC	41205	0.0	28.533468	2
TCAACGC	41460	0.0	28.357794	4
CAACGCA	41545	0.0	28.230413	5
AACGCAG	41790	0.0	28.107117	6
ACGCAGA	46660	0.0	25.12956	7
CGCAGAG	46960	0.0	25.00409	8
TACACAT	20665	0.0	24.542503	5
ACACATC	20730	0.0	23.872179	6
ATACACA	22035	0.0	23.838623	4
GCAGAGT	52705	0.0	22.137962	9
CACATCT	22705	0.0	21.668858	7
GAGTACT	33145	0.0	20.494337	12-13
GTACATG	34745	0.0	19.703627	1
TACATGG	34465	0.0	19.636757	2
>>END_MODULE