FastQCFastQC Report
Fri 27 May 2016
SRR2139124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10286730
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130350.047.08051
GTATCAA230150.035.285841
TCAACGC269650.029.4973964
ATCAACG273700.029.0436733
CAACGCA277750.028.5602665
AACGCAG287250.027.7054236
TATCAAC289300.027.7002122
ACGCAGA331600.024.0388077
CGCAGAG336800.023.65568
GTACATG317150.021.3814581
TACATGG321700.020.4783152
ACATGGG322400.019.7411773
GCAGAGT401550.019.726069
GAGTACT216400.019.43188912-13
GTGGTAT71200.018.9998631
CAGAGTA388050.018.00973710-11
AGAGTAC355750.017.97262210-11
TGGTATC68200.017.9465272
TATACCG7950.017.8277385
GTACTTT245700.017.09713214-15
Produced by FastQC (version 0.11.5)