##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10286730
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62030499488176	33.0	33.0	33.0	33.0	33.0
2	32.625295307643924	33.0	33.0	33.0	33.0	33.0
3	32.631157423204456	33.0	33.0	33.0	33.0	33.0
4	36.546772881177986	37.0	37.0	37.0	37.0	37.0
5	36.57184216947466	37.0	37.0	37.0	37.0	37.0
6	36.579322486348914	37.0	37.0	37.0	37.0	37.0
7	36.553766260026265	37.0	37.0	37.0	37.0	37.0
8	36.547049159451056	37.0	37.0	37.0	37.0	37.0
9	36.581505590211854	37.0	37.0	37.0	37.0	37.0
10-11	36.53279322000286	37.0	37.0	37.0	37.0	37.0
12-13	36.51843924162489	37.0	37.0	37.0	37.0	37.0
14-15	38.618433797718026	40.0	37.0	40.0	37.0	40.0
16-17	38.56057546956127	40.0	37.0	40.0	37.0	40.0
18-19	38.57272656130763	40.0	37.0	40.0	37.0	40.0
20-21	38.568006987820645	40.0	37.0	40.0	37.0	40.0
22-23	38.56512774622392	40.0	37.0	40.0	37.0	40.0
24-25	38.56238066580571	40.0	37.0	40.0	37.0	40.0
26-27	38.44382559249768	40.0	37.0	40.0	37.0	40.0
28-29	38.396146277484874	40.0	37.0	40.0	37.0	40.0
30-31	38.24742176519153	40.0	37.0	40.0	37.0	40.0
32-33	38.26462549089548	40.0	37.0	40.0	37.0	40.0
34-35	38.24109898663585	40.0	37.0	40.0	37.0	40.0
36-37	38.19817433528005	40.0	37.0	40.0	37.0	40.0
38-39	38.150210281029544	40.0	37.0	40.0	37.0	40.0
40-41	38.12117507708198	40.0	37.0	40.0	37.0	40.0
42-43	38.07432170425637	40.0	37.0	40.0	33.0	40.0
44-45	38.00255139438298	40.0	37.0	40.0	33.0	40.0
46-47	37.855351714213555	40.0	37.0	40.0	33.0	40.0
48-49	37.87151446310057	40.0	37.0	40.0	33.0	40.0
50-51	37.83793830425284	40.0	37.0	40.0	33.0	40.0
52-53	37.772952378628865	40.0	37.0	40.0	33.0	40.0
54-55	37.60368437920158	40.0	37.0	40.0	33.0	40.0
56-57	37.489184784314546	40.0	37.0	40.0	33.0	40.0
58-59	37.359592241308874	38.5	37.0	40.0	33.0	40.0
60-61	37.18990025374302	37.0	37.0	40.0	33.0	40.0
62-63	37.01666798080476	37.0	37.0	40.0	33.0	40.0
64-65	36.836329180023924	37.0	37.0	40.0	33.0	40.0
66-67	36.63152820117785	37.0	37.0	40.0	33.0	40.0
68-69	36.41526891410642	37.0	37.0	40.0	33.0	40.0
70-71	36.17570060368361	37.0	37.0	38.5	33.0	40.0
72-73	35.948575297243536	37.0	37.0	37.0	33.0	40.0
74-75	35.75478943593674	37.0	35.0	37.0	33.0	40.0
76-77	34.06666174630767	35.0	33.0	37.0	30.0	37.0
78-79	35.25569779185042	37.0	33.0	37.0	33.0	37.0
80-81	35.316145604997494	37.0	33.0	37.0	33.0	37.0
82-83	35.17599588119346	37.0	33.0	37.0	33.0	37.0
84-85	35.04778153603618	37.0	33.0	37.0	33.0	37.0
86-87	34.962796548677176	37.0	33.0	37.0	33.0	37.0
88-89	34.82803257534101	37.0	33.0	37.0	33.0	37.0
90-91	34.68097797096016	37.0	33.0	37.0	33.0	37.0
92-93	34.67979777113034	37.0	33.0	37.0	33.0	37.0
94-95	34.741252430372214	37.0	33.0	37.0	33.0	37.0
96-97	34.74771402413262	37.0	33.0	37.0	33.0	37.0
98-99	34.74690745861585	37.0	33.0	37.0	33.0	37.0
100	34.73597107786077	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	9.0
20	73.0
21	395.0
22	1377.0
23	3884.0
24	8881.0
25	17207.0
26	30073.0
27	48002.0
28	71049.0
29	99027.0
30	135264.0
31	181196.0
32	241090.0
33	325615.0
34	451567.0
35	661896.0
36	1184327.0
37	2986546.0
38	3736598.0
39	102651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73566332546883	19.94599838821472	14.211396624583323	24.106941661733124
2	14.744180123323932	20.78575990620926	41.61958173297053	22.85047823749627
3	18.309978000783534	25.963663865971014	30.201152358426825	25.525205774818627
4	12.721924265534334	17.970715669605404	38.37463411599216	30.932725948868107
5	13.660210776408052	37.511298537047246	35.28859025171264	13.539900434832061
6	30.326518708623755	37.360222423128896	18.72456667347157	13.588692194775778
7	26.724206817910062	32.378219317509064	23.006611430454576	17.890962434126298
8	24.48815123950954	36.73284902004816	20.95362666270039	17.825373077741908
9	25.73282277264009	16.754362173402043	20.964514476417676	36.54830057754019
10-11	24.535740765648523	26.49408441428743	28.644366875831007	20.32580794423304
12-13	25.373317218717027	24.049135159845537	28.669550155771113	21.907997465666316
14-15	22.4355731535399	25.377788011726395	26.270664381430475	25.915974453303225
16-17	22.11982816696851	28.446099975405208	26.95041572978002	22.483656127846263
18-19	22.196713630084584	27.63453983919088	28.561243466096613	21.607503064627924
20-21	22.9113403825762	26.788670238194477	28.44633679411672	21.853652585112595
22-23	22.696135761510373	26.77052010052584	28.27509243768067	22.258251700283115
24-25	22.47152568145122	26.940585031851143	28.31297578647911	22.274913500218528
26-27	22.354139464693375	27.048439299397465	28.29605234295989	22.301368892949277
28-29	22.305108592557872	27.094468550389916	28.343810527576768	22.25661232947545
30-31	22.613677735703952	26.957312738954126	28.11098867084479	22.318020854497135
32-33	22.38449049365105	27.105471395090614	28.18836874837788	22.321669362880453
34-35	22.51584577480071	27.2122399374648	28.127994722101036	22.143919565633453
36-37	22.478079046676793	27.12567195513831	28.040421445552653	22.355827552632242
38-39	22.351407015913434	27.196190931646548	28.127006743273675	22.32539530916634
40-41	22.607397329218156	27.05229026850795	27.984705102906265	22.355607299367634
42-43	22.492616435303088	27.18539725625285	27.792330488460564	22.529655819983503
44-45	22.543666775833294	27.268587225615864	27.668615338659443	22.519130659891403
46-47	22.69873690018084	27.262701102853352	27.338593990529603	22.6999680064362
48-49	22.591275646438742	27.38395791620778	27.32614658084509	22.698619856508383
50-51	22.61340179377369	27.534239436878376	27.230865142476706	22.62149362687122
52-53	22.759311383511424	27.40292193467692	27.172090783538945	22.66567589827271
54-55	22.690976843919973	27.32687042362295	27.194010618667903	22.788142113789174
56-57	22.72497055439695	27.48331590957833	27.098340065533215	22.693373470491508
58-59	22.66723459406473	27.478454775030166	27.21258299491296	22.641727635992144
60-61	22.771633648711674	27.495220432306276	27.15503819117182	22.578107727810227
62-63	22.710624405262937	27.580054049962726	27.09546353972977	22.61385800504457
64-65	22.72737994092838	27.513942223985556	27.038455329928233	22.720222505157828
66-67	22.726381895213553	27.478705645433237	26.979318071962155	22.81559438739105
68-69	22.68799126601401	27.45841228454117	27.068148162049	22.785448287395827
70-71	22.68491330097529	27.288669299885736	27.12005127654208	22.906366122596893
72-73	22.738470753093925	27.3186374929907	27.018970905360106	22.92392084855527
74-75	22.622821534477225	27.44653160073679	27.03333745582819	22.8973094089578
76-77	22.770636273407327	27.377875585206386	26.978785891319095	22.87270225006719
78-79	22.715637647258234	27.349610961951086	26.983157637799437	22.951593752991233
80-81	22.70156198122833	27.43387332137368	26.998931654706045	22.865633042691947
82-83	22.764000700936986	27.36756045949659	27.041554077435865	22.826884762130554
84-85	22.807183602699297	27.361202722238147	27.000115238154788	22.831498436907765
86-87	22.741065331968056	27.37182738470125	27.038621527851024	22.84848575547967
88-89	22.79217675675528	27.306569371897567	26.9975543629357	22.903699508411453
90-91	22.818656908928286	27.3851099790216	26.961132925818003	22.835100186232108
92-93	22.77691062966284	27.329621893745642	27.07220435318107	22.82126312341044
94-95	22.673528535791046	27.37909501125746	27.073125817245298	22.8742506357062
96-97	22.752130922653592	27.465305310375925	27.104815456103747	22.677748310866733
98-99	23.914869145759596	29.387751230768544	27.27747972523622	19.419899898235638
100	22.771845272282498	30.406496961635415	21.026643018789194	25.795014747292893
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1736.0
1	1628.0
2	2350.0
3	3361.0
4	4527.5
5	7526.5
6	10087.0
7	11575.5
8	12269.0
9	11787.0
10	10796.0
11	9796.0
12	10586.0
13	14107.5
14	22369.5
15	33928.0
16	43819.0
17	50319.0
18	52453.0
19	50248.5
20	45544.5
21	40064.0
22	36419.0
23	36891.0
24	41803.5
25	51195.0
26	64254.5
27	80110.5
28	98663.0
29	120583.0
30	144090.0
31	168383.0
32	193667.0
33	217953.5
34	241631.5
35	265135.0
36	289763.5
37	312984.0
38	333682.5
39	351904.5
40	367901.0
41	382300.0
42	392674.5
43	402453.0
44	410528.0
45	419302.0
46	426853.5
47	430614.0
48	435902.0
49	435166.0
50	428294.5
51	419219.0
52	405437.0
53	387519.0
54	367916.5
55	345229.0
56	319519.5
57	290985.5
58	259108.0
59	224367.0
60	186590.5
61	151421.5
62	121532.5
63	94474.0
64	70548.0
65	52986.0
66	40439.5
67	30804.0
68	23577.5
69	17152.5
70	12028.0
71	8604.0
72	6098.0
73	4165.5
74	2715.0
75	1742.5
76	1101.5
77	719.5
78	480.5
79	315.0
80	219.5
81	164.0
82	126.5
83	77.5
84	46.5
85	37.0
86	29.5
87	25.0
88	17.5
89	10.5
90	7.5
91	4.5
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.916378674272582E-4
7	0.0
8	0.0
9	0.0
10-11	0.006216747207324388
12-13	2.4303155618938184E-5
14-15	0.006838907991169205
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	179.0
20-21	570.0
22-23	1293.0
24-25	3394.0
26-27	3923.0
28-29	6519.0
30-31	17002.0
32-33	21779.0
34-35	30757.0
36-37	45860.0
38-39	55774.0
40-41	57753.0
42-43	69058.0
44-45	83169.0
46-47	80495.0
48-49	72848.0
50-51	65957.0
52-53	64941.0
54-55	70181.0
56-57	73958.0
58-59	75131.0
60-61	73705.0
62-63	72584.0
64-65	77681.0
66-67	83047.0
68-69	80945.0
70-71	80120.0
72-73	77114.0
74-75	79679.0
76-77	79560.0
78-79	84884.0
80-81	86911.0
82-83	84758.0
84-85	84049.0
86-87	89694.0
88-89	95503.0
90-91	99695.0
92-93	102122.0
94-95	124013.0
96-97	349280.0
98-99	636538.0
100-101	6844307.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.80931515459491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1157616041062	74.68730949520285
2	7.5145318452319785	12.595755353125732
3	1.842453593129577	4.632443215329375
4	0.6988220115192098	2.342711768015256
5	0.3145648831432613	1.3181733713960953
6	0.16722554707415083	0.8409035145982232
7	0.09544119832879512	0.5599203028629132
8	0.06207778200453343	0.41621571168929455
9	0.03776330365268362	0.2848424955395784
>10	0.1464333898755321	1.9549828396289874
>50	0.0037707326580806192	0.21677114170755543
>100	0.0011333512920636798	0.1389410141236802
>500	2.0757984034808124E-5	0.01102977678050578
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.721262247575275E-6
2	0.0	3.88850489903011E-5	0.0	0.0	9.721262247575275E-6
3	0.0	3.88850489903011E-5	0.0	0.0	9.721262247575275E-6
4	0.0	9.721262247575274E-5	0.0	0.0	9.721262247575275E-6
5	0.0	1.0693388472332802E-4	0.0	0.0	9.721262247575275E-6
6	0.0	1.0693388472332802E-4	0.0	0.0	9.721262247575275E-6
7	0.0	1.0693388472332802E-4	0.0	0.0	1.944252449515055E-5
8	0.0	1.555401959612044E-4	0.0	0.0	2.9163786742725822E-5
9	0.0	2.624740806845324E-4	0.0	0.0	3.88850489903011E-5
10-11	0.0	2.624740806845324E-4	0.0	0.0	3.88850489903011E-5
12-13	0.0	2.624740806845324E-4	0.0	0.0	3.88850489903011E-5
14-15	0.0	3.159410230461964E-4	0.0	0.0	3.88850489903011E-5
16-17	0.0	3.3052291641755933E-4	0.0	0.0	4.3745680114088733E-5
18-19	0.0	3.451048097889223E-4	0.0	0.0	5.8327573485451645E-5
20-21	0.0	3.6454733428407276E-4	0.0	0.0	5.8327573485451645E-5
22-23	0.0	3.791292276554357E-4	0.0	9.721262247575275E-6	6.318820460923928E-5
24-25	0.0	4.2287490776952443E-4	0.0	9.721262247575275E-6	6.804883573302691E-5
26-27	0.0	4.6662058788361316E-4	0.0	2.4303155618938187E-5	6.804883573302691E-5
28-29	0.0	4.6662058788361316E-4	0.0	1.312370403422662E-4	6.804883573302691E-5
30-31	0.0	5.638332103593659E-4	0.0	5.49251316988003E-4	7.290946685681456E-5
32-33	0.0	5.638332103593659E-4	0.0	0.0016623358443353718	8.749136022817747E-5
34-35	0.0	6.367426772161805E-4	0.0	0.0031594102304619643	1.1179451584711565E-4
36-37	0.0	7.145127751967827E-4	0.0	0.005375858022909127	1.1665514697090329E-4
38-39	0.0	7.388159308157208E-4	0.0	0.00919631408620621	1.3123704034226618E-4
40-41	0.0	7.777009798060219E-4	0.0	0.01275429606881876	1.3609767146605383E-4
42-43	0.0	8.068647665487477E-4	0.0	0.016176180379965256	1.4095830258984148E-4
44-45	0.0	9.040773890245005E-4	0.0	0.02028341367956581	1.4581893371362912E-4
46-47	0.0	9.818474870051028E-4	0.0	0.02470172737108877	1.6040082708499202E-4
48-49	0.0	9.818474870051028E-4	0.0	0.029431121454534144	1.7498272045635493E-4
50-51	0.0	0.0011373876829663071	0.0	0.03389804145729498	1.7498272045635493E-4
52-53	0.0	0.0011373876829663071	0.0	0.0382531669442087	1.944252449515055E-4
54-55	0.0	0.0011616908385852452	0.0	0.04250621917752288	2.1386776944665604E-4
56-57	0.0	0.001176272731956608	0.0	0.04727449830995856	2.1386776944665604E-4
58-59	0.0	0.001176272731956608	0.0	0.05226150584296467	2.235890316942313E-4
60-61	0.0	0.001205436518699334	0.0	0.05707353065551443	2.235890316942313E-4
62-63	0.0	0.0012151577809469093	0.0	0.06197304682829237	2.4303155618938185E-4
64-65	0.0	0.001312370403422662	0.0	0.06697949688579363	2.4789218731316947E-4
66-67	0.0	0.0013269522967940249	0.0	0.0721074627213896	2.527528184369571E-4
68-69	0.0	0.0013609767146605383	0.0	0.07740555064631813	2.527528184369571E-4
70-71	0.0	0.001365837345784326	0.0	0.0820911990496494	2.527528184369571E-4
72-73	9.721262247575275E-6	0.0013804192391556889	0.0	0.08557141093428135	2.8191660517968295E-4
74-75	9.721262247575275E-6	0.0014387468126411407	0.0	0.08839543761720196	2.8191660517968295E-4
76-77	9.721262247575275E-6	0.0015165169106217428	0.0	0.09143819270069303	2.8191660517968295E-4
78-79	9.721262247575275E-6	0.0015554019596120438	0.0	0.09331439631447505	2.8191660517968295E-4
80-81	9.721262247575275E-6	0.0015602625907358315	0.0	0.09391711457382472	2.8191660517968295E-4
82-83	9.721262247575275E-6	0.0015797051152309821	0.0	0.09433998948159425	2.8191660517968295E-4
84-85	9.721262247575275E-6	0.0016040082708499203	0.0	0.09461218482452635	2.867772363034706E-4
86-87	9.721262247575275E-6	0.0016234507953450709	0.0	0.09471425807812589	2.9163786742725825E-4
88	9.721262247575275E-6	0.0017109421555732483	0.0	0.09473370060262104	3.013591296748335E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13035	0.0	47.0805	1
GTATCAA	23015	0.0	35.28584	1
TCAACGC	26965	0.0	29.497396	4
ATCAACG	27370	0.0	29.043673	3
CAACGCA	27775	0.0	28.560266	5
AACGCAG	28725	0.0	27.705423	6
TATCAAC	28930	0.0	27.700212	2
ACGCAGA	33160	0.0	24.038807	7
CGCAGAG	33680	0.0	23.6556	8
GTACATG	31715	0.0	21.381458	1
TACATGG	32170	0.0	20.478315	2
ACATGGG	32240	0.0	19.741177	3
GCAGAGT	40155	0.0	19.72606	9
GAGTACT	21640	0.0	19.431889	12-13
GTGGTAT	7120	0.0	18.999863	1
CAGAGTA	38805	0.0	18.009737	10-11
AGAGTAC	35575	0.0	17.972622	10-11
TGGTATC	6820	0.0	17.946527	2
TATACCG	795	0.0	17.827738	5
GTACTTT	24570	0.0	17.097132	14-15
>>END_MODULE