FastQCFastQC Report
Fri 27 May 2016
SRR2139123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11899220
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162650.042.8025321
CTTATAC103300.038.9131741
GTATCAA272650.033.25061
TATACAC153500.030.0595283
TTATACA144950.028.6759072
TCAACGC313750.028.1611484
ATCAACG317550.027.8084683
CAACGCA321100.027.5441045
TATCAAC331750.026.8888652
AACGCAG334750.026.5742576
CGCAGAG382800.023.2052678
ACGCAGA383100.023.175747
GTACATG354500.021.8821581
TACACAT200750.021.6875555
ACACATC191650.021.2221836
TACATGG353300.021.1697272
ACATCTC196100.020.8280128
ACATGGG354500.020.6638013
GCAGAGT458100.019.4121959
GAGTACT243550.019.29926312-13
Produced by FastQC (version 0.11.5)