##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11899220
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.620788925660676	33.0	33.0	33.0	33.0	33.0
2	32.63125272076657	33.0	33.0	33.0	33.0	33.0
3	32.638809182450615	33.0	33.0	33.0	33.0	33.0
4	36.55365040733762	37.0	37.0	37.0	37.0	37.0
5	36.57516702775476	37.0	37.0	37.0	37.0	37.0
6	36.580671842356054	37.0	37.0	37.0	37.0	37.0
7	36.55688431678715	37.0	37.0	37.0	37.0	37.0
8	36.53489069031416	37.0	37.0	37.0	37.0	37.0
9	36.57090733678342	37.0	37.0	37.0	37.0	37.0
10-11	36.531204650388844	37.0	37.0	37.0	37.0	37.0
12-13	36.51878181931252	37.0	37.0	37.0	37.0	37.0
14-15	38.621680832861315	40.0	37.0	40.0	37.0	40.0
16-17	38.55989468217244	40.0	37.0	40.0	37.0	40.0
18-19	38.574626362064066	40.0	37.0	40.0	37.0	40.0
20-21	38.56809622891434	40.0	37.0	40.0	37.0	40.0
22-23	38.56989977617866	40.0	37.0	40.0	37.0	40.0
24-25	38.56502894038642	40.0	37.0	40.0	37.0	40.0
26-27	38.43839480240127	40.0	37.0	40.0	37.0	40.0
28-29	38.3929967959823	40.0	37.0	40.0	37.0	40.0
30-31	38.230925734278415	40.0	37.0	40.0	37.0	40.0
32-33	38.253701501427486	40.0	37.0	40.0	37.0	40.0
34-35	38.2351044067037	40.0	37.0	40.0	37.0	40.0
36-37	38.18978006976033	40.0	37.0	40.0	37.0	40.0
38-39	38.13549044672639	40.0	37.0	40.0	37.0	40.0
40-41	38.11881746063597	40.0	37.0	40.0	37.0	40.0
42-43	38.078215168839165	40.0	37.0	40.0	33.0	40.0
44-45	38.00893074851698	40.0	37.0	40.0	33.0	40.0
46-47	37.86693683490124	40.0	37.0	40.0	33.0	40.0
48-49	37.888817644449446	40.0	37.0	40.0	33.0	40.0
50-51	37.84213614784784	40.0	37.0	40.0	33.0	40.0
52-53	37.77737571084115	40.0	37.0	40.0	33.0	40.0
54-55	37.6163210876186	40.0	37.0	40.0	33.0	40.0
56-57	37.49875408546246	40.0	37.0	40.0	33.0	40.0
58-59	37.37317793086977	40.0	37.0	40.0	33.0	40.0
60-61	37.207033538768	37.0	37.0	40.0	33.0	40.0
62-63	37.038789084926385	37.0	37.0	40.0	33.0	40.0
64-65	36.86730356209084	37.0	37.0	40.0	33.0	40.0
66-67	36.66892576595108	37.0	37.0	40.0	33.0	40.0
68-69	36.45896567117755	37.0	37.0	40.0	33.0	40.0
70-71	36.22538303304553	37.0	37.0	38.5	33.0	40.0
72-73	35.99986091559761	37.0	37.0	37.0	33.0	40.0
74-75	35.803610473020356	37.0	37.0	37.0	33.0	40.0
76-77	34.13507317745601	35.0	33.0	37.0	30.0	37.0
78-79	35.300012279912266	37.0	33.0	37.0	33.0	37.0
80-81	35.352187375650615	37.0	33.0	37.0	33.0	37.0
82-83	35.20745822952662	37.0	33.0	37.0	33.0	37.0
84-85	35.078061654027806	37.0	33.0	37.0	33.0	37.0
86-87	34.99403995722936	37.0	33.0	37.0	33.0	37.0
88-89	34.860112289059806	37.0	33.0	37.0	33.0	37.0
90-91	34.713661878525905	37.0	33.0	37.0	33.0	37.0
92-93	34.71247247585485	37.0	33.0	37.0	33.0	37.0
94-95	34.77191799900436	37.0	33.0	37.0	33.0	37.0
96-97	34.781487495379366	37.0	33.0	37.0	33.0	37.0
98-99	34.78024226188026	37.0	33.0	37.0	33.0	37.0
100	34.76603366787386	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	2.0
17	5.0
18	12.0
19	15.0
20	91.0
21	406.0
22	1662.0
23	4721.0
24	10565.0
25	20580.0
26	35566.0
27	55899.0
28	81800.0
29	114619.0
30	155334.0
31	208839.0
32	278893.0
33	374831.0
34	519280.0
35	760364.0
36	1349411.0
37	3404479.0
38	4398538.0
39	123306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.22060941809631	20.108368447679766	14.342082926443918	24.328939207780007
2	14.451619517917983	20.642294200796353	41.92919367824109	22.97689260304457
3	18.117498457882114	26.079280826810496	30.395235990258186	25.407984725049204
4	12.66452759088411	18.175216526797556	38.228631792672125	30.931624089646213
5	13.512221809496758	37.66077104213553	35.34389649069435	13.483110657673361
6	30.00609200772119	37.593444330739295	18.68029414085673	13.720169520682783
7	26.525864720544707	32.59502723707941	22.740540976635444	18.138567065740443
8	24.352091985861257	36.81933773810384	21.090458030022134	17.738112246012765
9	25.383604975788327	16.903788651693137	20.884494950089167	36.82811142242937
10-11	24.372830887866822	26.511942253296873	28.663811587824643	20.451415271011662
12-13	25.31289243653595	23.91562822999194	28.67907190868105	22.092407424791062
14-15	22.36670604575042	25.46466037147616	26.138681022151754	26.029952560621666
16-17	22.14905262697891	28.597269400851484	26.732428680199206	22.5212492919704
18-19	22.173604656439664	27.71992618003533	28.312532250013028	21.793936913511978
20-21	22.894375629654455	26.752568254805333	28.26199928354647	22.091056831993747
22-23	22.629738029814696	26.983002207858036	28.132981149620896	22.254278612706365
24-25	22.493734944184965	27.20539989114777	28.12307405461366	22.177791110053608
26-27	22.516039911467054	27.309938968370208	28.030307616836865	22.14371350332587
28-29	22.24385866222699	27.29549210829358	28.203356121417443	22.257293108061987
30-31	22.854312143859225	27.126479530492524	27.958118987715135	22.061089337933115
32-33	22.1185131295239	27.544732111320226	28.090064158323973	22.246690600831897
34-35	22.392555044186015	27.476233773195315	28.164232198559663	21.96697898405901
36-37	22.31682409373978	27.42270725234327	27.987300325998977	22.27316832791798
38-39	22.294221757830197	27.15774843700712	28.19995984635814	22.348069958804547
40-41	22.450638537407375	27.01186585648408	28.170381238505126	22.36711436760342
42-43	22.4808955702799	27.086781922733984	28.03808745182337	22.39423505516275
44-45	22.455945362956246	27.270843759161444	27.82184936385179	22.451361514030513
46-47	22.67817040964158	27.104885601264257	27.405432866564823	22.811511122529343
48-49	22.534284365268412	27.235694290448475	27.45968989780562	22.770331446477485
50-51	22.436818533055135	27.37195176234654	27.521561578396476	22.669668126201852
52-53	22.568227548134168	27.398579411401258	27.532320058568256	22.50087298189632
54-55	22.516068704008497	27.364215031283468	27.34095411657001	22.778762148138025
56-57	22.732221799231695	27.51676681827538	27.11443148555285	22.636579896940077
58-59	22.458144295059526	27.72936920794776	27.410749916656325	22.401736580336387
60-61	22.721589171957397	27.826426411838877	27.07411128586714	22.37787313033658
62-63	22.634016513684564	27.98700550256567	26.98325223934664	22.39572574440313
64-65	22.566177762081125	27.828882135970375	27.015300774529116	22.589639327419388
66-67	22.68661956211176	27.654134018548316	26.974959348352538	22.684287070987388
68-69	22.62839545537195	27.51831564041169	27.108891903634646	22.744397000581717
70-71	22.631062000571106	27.337210169028552	27.166632056403433	22.86509577399691
72-73	22.676702158166666	27.404137974878147	27.07068223676407	22.848477630191123
74-75	22.60875068113669	27.506824231381188	27.095294715408436	22.789130372073686
76-77	22.72934955388167	27.409043980797342	27.011805960492367	22.84980050482862
78-79	22.618157305644488	27.429278246954507	27.00884828755841	22.943716159842594
80-81	22.674049044267143	27.474887843793333	27.044236711107118	22.80682640083241
82-83	22.677839498592895	27.49603974141782	27.09783708381048	22.72828367617881
84-85	22.78315693284119	27.460894446188867	27.008359533820364	22.74758908714958
86-87	22.66404467488097	27.4509274452647	27.089224709607247	22.795803170247087
88-89	22.754484765058468	27.35807266191623	27.04394337997531	22.843499193049993
90-91	22.73825245057434	27.44507033778128	27.009764862103385	22.80691234954099
92-93	22.808197100729792	27.38935110059157	27.09775624137815	22.70469555730049
94-95	22.60523413139563	27.476904158535948	27.145303440786527	22.772558269281898
96-97	22.718187165327343	27.52415723929755	27.181171098237712	22.576484497137393
98-99	23.82488942714801	29.48304134362764	27.310967289516597	19.381101939707754
100	22.734055869604923	30.464723060641756	21.08932811254104	25.711892957212285
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1880.0
1	1775.5
2	2545.0
3	3587.0
4	4900.0
5	8251.0
6	11047.0
7	12499.0
8	13031.0
9	12380.5
10	11294.0
11	10229.0
12	11213.0
13	15299.5
14	24747.0
15	37918.0
16	49073.5
17	56365.0
18	58748.5
19	56307.5
20	51166.5
21	45438.0
22	41530.5
23	41992.0
24	47770.0
25	58562.0
26	73670.5
27	91239.0
28	111577.5
29	136650.0
30	163442.5
31	192509.0
32	223747.5
33	254001.0
34	282034.0
35	308295.5
36	339266.0
37	370884.5
38	396480.0
39	416484.5
40	435818.0
41	454238.0
42	464690.5
43	473400.0
44	485558.0
45	498098.5
46	515248.5
47	533637.5
48	535501.0
49	529645.5
50	522994.0
51	503510.0
52	474966.0
53	443855.0
54	414932.0
55	385978.0
56	355880.0
57	322872.0
58	287284.5
59	248998.0
60	204441.0
61	163364.5
62	129940.0
63	100191.5
64	74613.0
65	55963.5
66	42813.5
67	33064.5
68	25158.5
69	18155.5
70	13234.5
71	9661.5
72	6695.5
73	4495.5
74	2940.5
75	1879.5
76	1201.0
77	741.0
78	477.5
79	326.0
80	205.5
81	130.5
82	104.0
83	84.0
84	63.0
85	47.0
86	36.5
87	26.5
88	17.5
89	13.0
90	8.5
91	3.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.932899803516533E-4
7	0.0
8	0.0
9	0.0
10-11	0.006508829990537195
12-13	2.5211736567606956E-5
14-15	0.0064836182539695884
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	200.0
20-21	727.0
22-23	1540.0
24-25	3218.0
26-27	4596.0
28-29	8127.0
30-31	19678.0
32-33	25451.0
34-35	37564.0
36-37	60752.0
38-39	69273.0
40-41	68729.0
42-43	79316.0
44-45	95626.0
46-47	92871.0
48-49	84718.0
50-51	76853.0
52-53	76881.0
54-55	87977.0
56-57	97197.0
58-59	96046.0
60-61	99463.0
62-63	98675.0
64-65	101040.0
66-67	110765.0
68-69	95011.0
70-71	92024.0
72-73	87430.0
74-75	89982.0
76-77	90586.0
78-79	96872.0
80-81	98368.0
82-83	95818.0
84-85	95168.0
86-87	101360.0
88-89	108114.0
90-91	113117.0
92-93	116148.0
94-95	140510.0
96-97	397838.0
98-99	726546.0
100-101	7857045.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.34151472816639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.15905153433691	72.59149840335782
2	7.975170088669368	13.13375170631602
3	2.0825972748783292	5.144526425466993
4	0.7801354969611989	2.5695015405198385
5	0.39810595224422995	1.6390323565044482
6	0.19638300499077196	0.9702284458685533
7	0.1155516017314561	0.6660285741083736
8	0.0753435148056412	0.4963119307232423
9	0.04727001367460296	0.3503056074469152
>10	0.16617290975216353	2.1316603299634753
>50	0.0034621744123385276	0.19454097755526317
>100	7.376760528987941E-4	0.09954706171090963
>500	1.875749011669903E-5	0.013066640458066369
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.403912189202317E-6
2	0.0	0.0	0.0	0.0	8.403912189202317E-6
3	0.0	0.0	0.0	8.403912189202317E-6	8.403912189202317E-6
4	0.0	5.0423473135213904E-5	0.0	8.403912189202317E-6	8.403912189202317E-6
5	0.0	5.0423473135213904E-5	0.0	1.6807824378404635E-5	8.403912189202317E-6
6	0.0	5.0423473135213904E-5	0.0	1.6807824378404635E-5	8.403912189202317E-6
7	0.0	6.723129751361854E-5	0.0	1.6807824378404635E-5	1.6807824378404635E-5
8	0.0	9.24430340812255E-5	0.0	1.6807824378404635E-5	1.6807824378404635E-5
9	0.0	1.5967433159484403E-4	0.0	4.201956094601159E-5	1.6807824378404635E-5
10-11	0.0	1.5967433159484403E-4	0.0	5.462542922981506E-5	1.6807824378404635E-5
12-13	1.6807824378404635E-5	1.5967433159484403E-4	0.0	7.563520970282087E-5	1.6807824378404635E-5
14-15	1.6807824378404635E-5	2.1850171691926025E-4	0.0	8.824107798662434E-5	3.361564875680927E-5
16-17	1.6807824378404635E-5	2.2690562910846257E-4	0.0	1.0925085845963012E-4	5.0423473135213904E-5
18-19	1.6807824378404635E-5	2.479154095814684E-4	0.0	1.1345281455423129E-4	5.0423473135213904E-5
20-21	1.6807824378404635E-5	2.6052127786527186E-4	0.0	1.302606389326359E-4	5.0423473135213904E-5
22-23	1.6807824378404635E-5	2.6892519005447416E-4	0.0	1.5547237550024286E-4	5.0423473135213904E-5
24-25	1.6807824378404635E-5	3.151467070950869E-4	0.0	2.0589584863545677E-4	5.0423473135213904E-5
26-27	1.6807824378404635E-5	3.361564875680927E-4	0.0	2.3951149739226605E-4	5.0423473135213904E-5
28-29	1.6807824378404635E-5	3.361564875680927E-4	0.0	3.44560399757295E-4	5.0423473135213904E-5
30-31	2.1009780473005792E-5	3.69772136324902E-4	0.0	8.319873067310294E-4	5.462542922981506E-5
32-33	2.5211736567606952E-5	3.823780046087054E-4	0.0	0.0022648543349900246	7.563520970282085E-5
34-35	2.5211736567606952E-5	4.5801321431152627E-4	0.0	0.003966646553303494	9.24430340812255E-5
36-37	2.5211736567606952E-5	5.29446467919746E-4	0.0	0.006437396736928975	9.24430340812255E-5
38-39	2.5211736567606952E-5	5.378503801089483E-4	0.0	0.010702382172949151	1.0084694627042781E-4
40-41	2.5211736567606952E-5	5.882738532441622E-4	0.0	0.01515645563322638	1.1765477064883245E-4
42-43	2.5211736567606952E-5	6.260914580955726E-4	0.0	0.019307988254692324	1.218567267434336E-4
44-45	3.7817604851410427E-5	7.017266677983936E-4	0.0	0.024245286665848687	1.302606389326359E-4
46-47	5.0423473135213904E-5	7.39544272649804E-4	0.0	0.029422096574397313	1.3446259502723708E-4
48-49	5.0423473135213904E-5	7.437462287444051E-4	0.0	0.0351787764240009	1.512704194056417E-4
50-51	5.0423473135213904E-5	7.94169701879619E-4	0.0	0.040397605893495545	1.512704194056417E-4
52-53	5.0423473135213904E-5	7.983716579742201E-4	0.0	0.0456752627483146	1.5547237550024286E-4
54-55	5.0423473135213904E-5	8.025736140688213E-4	0.0	0.05089409221780923	1.6807824378404633E-4
56-57	5.0423473135213904E-5	8.067755701634225E-4	0.0	0.055596081087667934	1.7648215597324866E-4
58-59	5.0423473135213904E-5	8.109775262580236E-4	0.0	0.060747679259648954	1.7648215597324866E-4
60-61	5.0423473135213904E-5	8.235833945418271E-4	0.0	0.06554631311968348	1.7648215597324866E-4
62-63	5.0423473135213904E-5	8.319873067310294E-4	0.0	0.07032813915533959	1.7648215597324866E-4
64-65	5.0423473135213904E-5	9.160264286230526E-4	0.0	0.07507634954223891	1.7648215597324866E-4
66-67	5.0423473135213904E-5	9.160264286230526E-4	0.0	0.08005566751434129	1.7648215597324866E-4
68-69	5.462542922981506E-5	9.412381651906596E-4	0.0	0.08536694001791714	1.7648215597324866E-4
70-71	5.8827385324416225E-5	9.622479456636654E-4	0.0	0.09019498757061387	1.7648215597324866E-4
72-73	5.8827385324416225E-5	9.706518578528676E-4	0.0	0.09411121065078215	2.0169389254085562E-4
74-75	5.8827385324416225E-5	9.958635944204747E-4	0.0	0.09680466450742149	2.0169389254085562E-4
76-77	5.8827385324416225E-5	0.0010462870675556884	0.0	0.09968720638831788	2.0169389254085562E-4
78-79	6.302934141901739E-5	0.0010672968480286943	0.0	0.10165372184059124	2.0169389254085562E-4
80-81	6.723129751361854E-5	0.0010714988041232954	0.0	0.1022125820011732	2.0169389254085562E-4
82-83	6.723129751361854E-5	0.0010757007602178966	0.0	0.10254033457655207	2.0169389254085562E-4
84-85	6.723129751361854E-5	0.0011009124967855037	0.0	0.10272101868861992	2.0169389254085562E-4
86-87	7.143325360821969E-5	0.0011093164089747058	0.0	0.10278404803003895	2.0589584863545677E-4
88	7.563520970282085E-5	0.001168143794299122	0.0	0.10278824998613355	2.1009780473005795E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16265	0.0	42.802532	1
CTTATAC	10330	0.0	38.913174	1
GTATCAA	27265	0.0	33.2506	1
TATACAC	15350	0.0	30.059528	3
TTATACA	14495	0.0	28.675907	2
TCAACGC	31375	0.0	28.161148	4
ATCAACG	31755	0.0	27.808468	3
CAACGCA	32110	0.0	27.544104	5
TATCAAC	33175	0.0	26.888865	2
AACGCAG	33475	0.0	26.574257	6
CGCAGAG	38280	0.0	23.205267	8
ACGCAGA	38310	0.0	23.17574	7
GTACATG	35450	0.0	21.882158	1
TACACAT	20075	0.0	21.687555	5
ACACATC	19165	0.0	21.222183	6
TACATGG	35330	0.0	21.169727	2
ACATCTC	19610	0.0	20.828012	8
ACATGGG	35450	0.0	20.663801	3
GCAGAGT	45810	0.0	19.412195	9
GAGTACT	24355	0.0	19.299263	12-13
>>END_MODULE