FastQCFastQC Report
Fri 27 May 2016
SRR2139122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11618402
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161150.044.37431
GTATCAA269400.034.3968961
TCAACGC315700.028.782724
ATCAACG318800.028.5003853
CAACGCA326800.027.7010145
TATCAAC335400.027.33342
AACGCAG335700.027.0178556
ACGCAGA381450.023.7772067
CGCAGAG386650.023.4695638
GTACATG355700.021.929211
TACATGG359400.021.296662
ACATGGG364400.020.4380323
GCAGAGT458650.019.7589959
GAGTACT244050.019.18998712-13
CATGGGG280550.018.8232694
CTTATAC67300.018.72071
CAGAGTA435950.018.3610-11
AGAGTAC400200.017.92594110-11
TATACAC101950.017.8833223
GTACTTT267700.017.51896914-15
Produced by FastQC (version 0.11.5)