##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139122_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11618402
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6151277086126	33.0	33.0	33.0	33.0	33.0
2	32.62185720549177	33.0	33.0	33.0	33.0	33.0
3	32.62836025126347	33.0	33.0	33.0	33.0	33.0
4	36.54279745183546	37.0	37.0	37.0	37.0	37.0
5	36.566666052698125	37.0	37.0	37.0	37.0	37.0
6	36.57410476931337	37.0	37.0	37.0	37.0	37.0
7	36.54969297843197	37.0	37.0	37.0	37.0	37.0
8	36.54035503333419	37.0	37.0	37.0	37.0	37.0
9	36.5757368354099	37.0	37.0	37.0	37.0	37.0
10-11	36.52635762646189	37.0	37.0	37.0	37.0	37.0
12-13	36.51289123065288	37.0	37.0	37.0	37.0	37.0
14-15	38.60766235322207	40.0	37.0	40.0	37.0	40.0
16-17	38.54670246390167	40.0	37.0	40.0	37.0	40.0
18-19	38.556778935691845	40.0	37.0	40.0	37.0	40.0
20-21	38.55284633327065	40.0	37.0	40.0	37.0	40.0
22-23	38.548687394607896	40.0	37.0	40.0	37.0	40.0
24-25	38.54734636423255	40.0	37.0	40.0	37.0	40.0
26-27	38.42667350808483	40.0	37.0	40.0	37.0	40.0
28-29	38.375034803573996	40.0	37.0	40.0	37.0	40.0
30-31	38.22510169587636	40.0	37.0	40.0	37.0	40.0
32-33	38.24188059366343	40.0	37.0	40.0	37.0	40.0
34-35	38.220721734048766	40.0	37.0	40.0	37.0	40.0
36-37	38.17533166600574	40.0	37.0	40.0	37.0	40.0
38-39	38.12198128283382	40.0	37.0	40.0	37.0	40.0
40-41	38.0923182265952	40.0	37.0	40.0	33.0	40.0
42-43	38.04613619674118	40.0	37.0	40.0	33.0	40.0
44-45	37.97186789985578	40.0	37.0	40.0	33.0	40.0
46-47	37.82478829974366	40.0	37.0	40.0	33.0	40.0
48-49	37.8364079891166	40.0	37.0	40.0	33.0	40.0
50-51	37.79454902774484	40.0	37.0	40.0	33.0	40.0
52-53	37.72765307755856	40.0	37.0	40.0	33.0	40.0
54-55	37.556053624498446	40.0	37.0	40.0	33.0	40.0
56-57	37.43662395111098	40.0	37.0	40.0	33.0	40.0
58-59	37.30427098049503	38.5	37.0	40.0	33.0	40.0
60-61	37.13142605867517	37.0	37.0	40.0	33.0	40.0
62-63	36.95554164153737	37.0	37.0	40.0	33.0	40.0
64-65	36.77366248162627	37.0	37.0	40.0	33.0	40.0
66-67	36.5671614484107	37.0	37.0	40.0	33.0	40.0
68-69	36.35030775810942	37.0	37.0	40.0	33.0	40.0
70-71	36.111326027863676	37.0	37.0	37.0	33.0	40.0
72-73	35.884516271267444	37.0	37.0	37.0	33.0	40.0
74-75	35.690147552904904	37.0	35.0	37.0	33.0	40.0
76-77	34.007399127625106	35.0	33.0	37.0	30.0	37.0
78-79	35.20127316865285	37.0	33.0	37.0	33.0	37.0
80-81	35.26464766239191	37.0	33.0	37.0	33.0	37.0
82-83	35.12886826279781	37.0	33.0	37.0	33.0	37.0
84-85	35.00180917069657	37.0	33.0	37.0	33.0	37.0
86-87	34.919209186893774	37.0	33.0	37.0	33.0	37.0
88-89	34.78717392716447	37.0	33.0	37.0	33.0	37.0
90-91	34.64350896979144	37.0	33.0	37.0	33.0	37.0
92-93	34.64243081157066	37.0	33.0	37.0	33.0	37.0
94-95	34.70176334444204	37.0	33.0	37.0	33.0	37.0
96-97	34.708287602454476	37.0	33.0	37.0	33.0	37.0
98-99	34.708807475092286	37.0	33.0	37.0	33.0	37.0
100	34.69838156457816	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	25.0
20	84.0
21	417.0
22	1618.0
23	4659.0
24	10415.0
25	20338.0
26	35364.0
27	55862.0
28	82576.0
29	115734.0
30	156692.0
31	209242.0
32	278257.0
33	374098.0
34	519683.0
35	763104.0
36	1363192.0
37	3405357.0
38	4113001.0
39	108682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83706158557778	19.794692936257498	14.216748568348727	24.151496909815997
2	14.841447214513664	20.622001201197893	41.4745332447612	23.062018339527242
3	18.462065609366935	25.63020284545155	30.085875837313942	25.82185570786757
4	12.776180407598222	17.87615026575944	38.04491357761592	31.302755749026417
5	13.810573949842672	37.43348698039541	35.10363989815467	13.652299171607249
6	30.67475844308759	37.081908148984624	18.58648968272685	13.656843725200932
7	27.124160448226874	32.224001200853614	22.59263365134035	18.05920469957917
8	24.739581226402734	36.60660046020098	20.726103297165995	17.927715016230287
9	25.791423449750102	16.77272113434542	20.71235964398199	36.72349577192249
10-11	24.731501553883675	26.385861530694854	28.404335122419177	20.47830179300229
12-13	25.5755342570606	23.846070722041297	28.44631238181194	22.132082639086164
14-15	22.600102060179008	25.2402173571079	26.036957284539895	26.1227232981732
16-17	22.341260872192233	28.348166985442575	26.685662107405133	22.62491003496006
18-19	22.349880818377603	27.49784781074024	28.29907675771591	21.853194613166252
20-21	23.11827871378573	26.63650452683862	28.202472950024514	22.04274380935113
22-23	22.88698074538166	26.612709754208215	28.042755732084462	22.457553768325667
24-25	22.64717604300683	26.807042068107922	28.084452022189442	22.461329866695802
26-27	22.596303448912604	26.942318827423627	28.075936899512417	22.385440824151353
28-29	22.451669821033228	26.93785674567511	28.114149482725097	22.496323950566566
30-31	22.81372815131676	26.769235346481906	28.013055580648437	22.403980921552897
32-33	22.49377301845061	27.00724128897492	27.979404149190117	22.519581543384344
34-35	22.665242496155944	27.044324384979042	28.00004190604267	22.29039121282235
36-37	22.73098254107648	26.944167398991798	27.848409588745927	22.4764404711858
38-39	22.537762161928637	27.012993190406565	27.941060763986243	22.508183883678555
40-41	22.749561258054058	26.891738875570237	27.858412387902593	22.500287478473115
42-43	22.684503143944752	26.95648735982021	27.682298868042217	22.67671062819282
44-45	22.715498286294874	27.076500998431875	27.500319064485158	22.707681650788096
46-47	22.92168395079606	27.056362164190688	27.16846586968389	22.85348801532937
48-49	22.752461556878085	27.193611347137015	27.190396267183804	22.8635308288011
50-51	22.753552950350944	27.345165063841435	27.134318028944943	22.76696395686267
52-53	22.90506647169779	27.238825612809354	27.077524938960075	22.778582976532782
54-55	22.852023546983265	27.13200280428326	27.0687257710719	22.947247877661574
56-57	22.886086435440653	27.3070760621012	26.979156968530326	22.827680533927825
58-59	22.802152634212852	27.380036142441828	27.056941638797138	22.760869584548185
60-61	22.92883400469819	27.388794080402818	26.97905441673722	22.70331749816178
62-63	22.839313723863334	27.501564423509283	26.932109986877435	22.72701186574995
64-65	22.848067875397163	27.436875788621307	26.86565611950965	22.849400216471878
66-67	22.876379067791	27.377008118165456	26.81322930429586	22.93338350974768
68-69	22.865310422718625	27.32632708811618	26.898334777324045	22.910027711841146
70-71	22.874021047223252	27.14613934514774	26.950593627957716	23.0292459796713
72-73	22.916171466164823	27.176618434386008	26.89244036594498	23.01476973350419
74-75	22.791765773813264	27.317044523380257	26.880726921976056	23.010462780830423
76-77	22.93536722470995	27.222549206735795	26.837752439382196	23.00433112917206
78-79	22.879392624176013	27.259807863111067	26.79340888152068	23.067390631192236
80-81	22.919828006961207	27.29945061067534	26.804746479187123	22.975974903176326
82-83	22.927419630246977	27.25062363875064	26.854689507145146	22.96726722385724
84-85	22.989769830368452	27.245590729181046	26.83111953463252	22.933519905817988
86-87	22.89893613355369	27.22210164331671	26.909336338063056	22.969625885066545
88-89	22.935463960190464	27.219476964713152	26.835652431766743	23.00940664332964
90-91	22.98615813829194	27.26719213711803	26.812593243963732	22.934056480626296
92-93	22.946014663510827	27.211377492216304	26.911261632309902	22.931346211962968
94-95	22.877799185929042	27.23560925461826	26.91551792932514	22.971073630127556
96-97	22.972815966694792	27.32720679416071	26.948597872358754	22.751379366785745
98-99	24.081537551199194	29.282057035710718	27.109474444589598	19.526930968500487
100	22.9837046237829	30.24481381082753	20.884100889888973	25.887380675500598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1880.0
1	1712.0
2	2404.0
3	3457.5
4	4710.5
5	7812.5
6	10442.5
7	11968.5
8	12691.5
9	12424.5
10	11560.5
11	10521.5
12	11428.5
13	15184.5
14	23991.0
15	36738.5
16	47830.5
17	54669.0
18	56847.0
19	54612.0
20	49362.0
21	43196.0
22	38741.0
23	38487.5
24	42875.5
25	51978.5
26	65310.0
27	81525.0
28	101278.5
29	125418.5
30	150625.5
31	176788.0
32	205761.0
33	233782.5
34	260842.0
35	286631.5
36	312795.5
37	340519.5
38	366226.5
39	387004.0
40	406721.0
41	426321.0
42	440948.5
43	453990.0
44	466299.5
45	479306.5
46	492749.0
47	503131.5
48	505466.5
49	499369.0
50	491884.5
51	481701.5
52	465633.5
53	444294.5
54	422443.5
55	397980.0
56	368888.0
57	336334.5
58	300716.5
59	261820.5
60	219397.0
61	180492.0
62	145606.0
63	113041.0
64	84783.5
65	63566.5
66	48682.5
67	37491.0
68	28376.0
69	20344.5
70	14581.5
71	10734.0
72	7636.5
73	5264.0
74	3571.5
75	2447.0
76	1624.5
77	987.0
78	615.5
79	402.0
80	269.5
81	185.0
82	131.5
83	98.0
84	75.0
85	54.0
86	34.0
87	21.0
88	15.5
89	10.5
90	5.5
91	2.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.893547839022957E-4
7	0.0
8	0.0
9	8.607035631922532E-6
10-11	0.0063433852607269045
12-13	1.2910553447883797E-5
14-15	0.00642515209923017
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	259.0
20-21	706.0
22-23	1529.0
24-25	3923.0
26-27	4608.0
28-29	7371.0
30-31	18910.0
32-33	24032.0
34-35	33508.0
36-37	50678.0
38-39	61011.0
40-41	62814.0
42-43	75460.0
44-45	91320.0
46-47	88685.0
48-49	80354.0
50-51	72931.0
52-53	71877.0
54-55	79486.0
56-57	84544.0
58-59	84443.0
60-61	84136.0
62-63	81922.0
64-65	87372.0
66-67	94093.0
68-69	90034.0
70-71	89465.0
72-73	84631.0
74-75	87542.0
76-77	88092.0
78-79	93897.0
80-81	96246.0
82-83	93031.0
84-85	93800.0
86-87	99829.0
88-89	107347.0
90-91	112192.0
92-93	115774.0
94-95	139327.0
96-97	395542.0
98-99	721454.0
100-101	7764227.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.92590048831549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.66991049668366	73.5303217415583
2	7.648426513144935	12.685053118425014
3	1.9702609895648426	4.901570002699924
4	0.7564073793565327	2.5090305227658933
5	0.36177983986941786	1.500045949984503
6	0.18129485128353232	0.9020423277949293
7	0.12078869580082213	0.701155795766467
8	0.07586569414858893	0.5032984802754291
9	0.048852987297930216	0.36460601669025916
>10	0.1611328555518761	2.0416935972428103
>50	0.004281465787145229	0.23478847530872413
>100	9.79108362213005E-4	0.11648269455399635
>500	1.912314849827566E-5	0.009911276933695543
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.607035631922532E-6
2	0.0	2.5821106895767593E-5	0.0	0.0	8.607035631922532E-6
3	0.0	2.5821106895767593E-5	0.0	0.0	8.607035631922532E-6
4	0.0	7.746332068730278E-5	0.0	1.7214071263845063E-5	8.607035631922532E-6
5	0.0	8.607035631922531E-5	0.0	1.7214071263845063E-5	8.607035631922532E-6
6	0.0	8.607035631922531E-5	0.0	1.7214071263845063E-5	8.607035631922532E-6
7	0.0	9.467739195114784E-5	0.0	1.7214071263845063E-5	8.607035631922532E-6
8	0.0	1.377125701107605E-4	0.0	3.4428142527690127E-5	8.607035631922532E-6
9	0.0	2.1517589079806328E-4	0.0	6.0249249423457716E-5	2.5821106895767593E-5
10-11	4.303517815961266E-6	2.1517589079806328E-4	0.0	8.607035631922531E-5	2.5821106895767593E-5
12-13	8.607035631922532E-6	2.1517589079806328E-4	0.0	9.037387413518658E-5	2.5821106895767593E-5
14-15	8.607035631922532E-6	3.0124624711728857E-4	0.0	1.0758794539903164E-4	3.0124624711728858E-5
16-17	8.607035631922532E-6	3.0124624711728857E-4	0.0	1.2910553447883797E-4	4.3035178159612657E-5
18-19	8.607035631922532E-6	3.098532827492111E-4	0.0	1.3340905229479924E-4	6.0249249423457716E-5
20-21	8.607035631922532E-6	3.1846031838113364E-4	0.0	1.635336770065281E-4	6.455276723941898E-5
22-23	8.607035631922532E-6	3.614954965407463E-4	0.0	1.764442304544119E-4	9.037387413518658E-5
24-25	8.607035631922532E-6	4.1744122814824276E-4	0.0	1.979618195342182E-4	1.2910553447883797E-4
26-27	8.607035631922532E-6	4.4756585285997164E-4	0.0	2.3238996206190833E-4	1.2910553447883797E-4
28-29	8.607035631922532E-6	4.518693706759329E-4	0.0	3.4428142527690125E-4	1.2910553447883797E-4
30-31	8.607035631922532E-6	5.078151022834293E-4	0.0	6.885628505538025E-4	1.3340905229479924E-4
32-33	8.607035631922532E-6	5.078151022834293E-4	0.0	0.0016912825016727772	1.7214071263845063E-4
34-35	8.607035631922532E-6	5.895819407866934E-4	0.0	0.002982337846461157	1.8074774827037316E-4
36-37	8.607035631922532E-6	6.756522971059188E-4	0.0	0.004979170113067184	1.8074774827037316E-4
38-39	8.607035631922532E-6	6.885628505538025E-4	0.0	0.008301485866989282	1.8505126608633443E-4
40-41	8.607035631922532E-6	7.746332068730278E-4	0.0	0.011817459922629635	1.8935478390229567E-4
42-43	8.607035631922532E-6	8.004543137687954E-4	0.0	0.015273184728846532	1.8935478390229567E-4
44-45	8.607035631922532E-6	8.994352235359045E-4	0.0	0.019438989974697035	1.8935478390229567E-4
46-47	8.607035631922532E-6	9.812020620391685E-4	0.0	0.024086789215935205	2.0656885516614075E-4
48-49	8.607035631922532E-6	9.941126154870525E-4	0.0	0.028308540193393204	2.2378292642998582E-4
50-51	8.607035631922532E-6	0.0011103075965180065	0.0	0.032728252990385426	2.2378292642998582E-4
52-53	8.607035631922532E-6	0.0011103075965180065	0.0	0.036885451200604005	2.4099699769383087E-4
54-55	8.607035631922532E-6	0.0011232181499658904	0.0	0.04139553787173141	2.625145867736372E-4
56-57	8.607035631922532E-6	0.0011533427746776193	0.0	0.04650381351927743	2.75425140221521E-4
58-59	8.607035631922532E-6	0.0011576462924935806	0.0	0.051594875095559614	2.8403217585344355E-4
60-61	8.607035631922532E-6	0.0011705568459414641	0.0	0.05675479295689717	2.8403217585344355E-4
62-63	8.607035631922532E-6	0.0011963779528372318	0.0	0.061660803267093015	2.926392114853661E-4
64-65	8.607035631922532E-6	0.00135991162984376	0.0	0.06666579448705597	2.9694272930132735E-4
66-67	8.607035631922532E-6	0.0014115538436352952	0.0	0.07197633547195217	3.0554976493324984E-4
68-69	8.607035631922532E-6	0.0014373749505310627	0.0	0.07756660511488585	3.098532827492111E-4
70-71	8.607035631922532E-6	0.0014761066108747141	0.0	0.0822488324986517	3.270673540130562E-4
72-73	8.607035631922532E-6	0.0014804101286906754	0.0	0.08587669801750705	3.873166034365139E-4
74-75	8.607035631922532E-6	0.0015105347534024043	0.0	0.08903117657660667	3.873166034365139E-4
76-77	8.607035631922532E-6	0.001605212145353552	0.0	0.09191883703111667	3.873166034365139E-4
78-79	8.607035631922532E-6	0.0016267297344333585	0.0	0.09383820597703539	3.873166034365139E-4
80-81	8.607035631922532E-6	0.0016310332522493196	0.0	0.09446221606034977	3.959236390684364E-4
82-83	8.607035631922532E-6	0.0016482473235131649	0.0	0.09481080100344264	4.6047640630785544E-4
84-85	8.607035631922532E-6	0.001652550841329126	0.0	0.09500876282297686	4.6477992412381666E-4
86-87	8.607035631922532E-6	0.0016611578769610486	0.0	0.09513786835745569	4.733869597557392E-4
88	8.607035631922532E-6	0.0017644423045441188	0.0	0.0951593859465355	4.8199399538766173E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16115	0.0	44.3743	1
GTATCAA	26940	0.0	34.396896	1
TCAACGC	31570	0.0	28.78272	4
ATCAACG	31880	0.0	28.500385	3
CAACGCA	32680	0.0	27.701014	5
TATCAAC	33540	0.0	27.3334	2
AACGCAG	33570	0.0	27.017855	6
ACGCAGA	38145	0.0	23.777206	7
CGCAGAG	38665	0.0	23.469563	8
GTACATG	35570	0.0	21.92921	1
TACATGG	35940	0.0	21.29666	2
ACATGGG	36440	0.0	20.438032	3
GCAGAGT	45865	0.0	19.758995	9
GAGTACT	24405	0.0	19.189987	12-13
CATGGGG	28055	0.0	18.823269	4
CTTATAC	6730	0.0	18.7207	1
CAGAGTA	43595	0.0	18.36	10-11
AGAGTAC	40020	0.0	17.925941	10-11
TATACAC	10195	0.0	17.883322	3
GTACTTT	26770	0.0	17.518969	14-15
>>END_MODULE