FastQCFastQC Report
Fri 27 May 2016
SRR2139121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10061043
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132600.048.7943231
GTATCAA233300.036.6565361
TCAACGC279700.029.9461024
ATCAACG285650.029.3791733
CAACGCA287450.029.1394445
TATCAAC296350.028.5516032
AACGCAG296650.028.2794276
ACGCAGA339600.024.6774237
CGCAGAG348350.024.0452848
GTACATG314300.022.1799281
TACATGG315200.021.7192122
ACATGGG318950.020.8273853
GCAGAGT407000.020.6254029
GAGTACT226950.020.1284112-13
GTGGTAT68400.019.7646561
TGGTATC65000.019.469522
CATGGGG244150.019.2144554
CAGAGTA397250.018.62360410-11
AGAGTAC369000.018.3049510-11
GTACTTT251350.018.20070514-15
Produced by FastQC (version 0.11.5)