##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139121_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10061043
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.622869318817145	33.0	33.0	33.0	33.0	33.0
2	32.62445692757699	33.0	33.0	33.0	33.0	33.0
3	32.628656392781544	33.0	33.0	33.0	33.0	33.0
4	36.54376936864299	37.0	37.0	37.0	37.0	37.0
5	36.56851441744161	37.0	37.0	37.0	37.0	37.0
6	36.57613559548449	37.0	37.0	37.0	37.0	37.0
7	36.551185697148895	37.0	37.0	37.0	37.0	37.0
8	36.54257684814586	37.0	37.0	37.0	37.0	37.0
9	36.57683751078293	37.0	37.0	37.0	37.0	37.0
10-11	36.5274003401039	37.0	37.0	37.0	37.0	37.0
12-13	36.51312334118839	37.0	37.0	37.0	37.0	37.0
14-15	38.60788866522089	40.0	37.0	40.0	37.0	40.0
16-17	38.548569268613605	40.0	37.0	40.0	37.0	40.0
18-19	38.562924887608574	40.0	37.0	40.0	37.0	40.0
20-21	38.558238465363026	40.0	37.0	40.0	37.0	40.0
22-23	38.55405228703352	40.0	37.0	40.0	37.0	40.0
24-25	38.55291528825071	40.0	37.0	40.0	37.0	40.0
26-27	38.431015710784116	40.0	37.0	40.0	37.0	40.0
28-29	38.3826543382817	40.0	37.0	40.0	37.0	40.0
30-31	38.231451743026064	40.0	37.0	40.0	37.0	40.0
32-33	38.24767742892719	40.0	37.0	40.0	37.0	40.0
34-35	38.22565088349117	40.0	37.0	40.0	37.0	40.0
36-37	38.180283461521434	40.0	37.0	40.0	37.0	40.0
38-39	38.13122223512523	40.0	37.0	40.0	37.0	40.0
40-41	38.09829513629094	40.0	37.0	40.0	33.0	40.0
42-43	38.04657467276893	40.0	37.0	40.0	33.0	40.0
44-45	37.97130633775649	40.0	37.0	40.0	33.0	40.0
46-47	37.81740879618205	40.0	37.0	40.0	33.0	40.0
48-49	37.82662797117428	40.0	37.0	40.0	33.0	40.0
50-51	37.7931355662987	40.0	37.0	40.0	33.0	40.0
52-53	37.72376710698205	40.0	37.0	40.0	33.0	40.0
54-55	37.551657809100234	40.0	37.0	40.0	33.0	40.0
56-57	37.43482066053024	40.0	37.0	40.0	33.0	40.0
58-59	37.30091336797693	38.5	37.0	40.0	33.0	40.0
60-61	37.129236724478766	37.0	37.0	40.0	33.0	40.0
62-63	36.95341229043618	37.0	37.0	40.0	33.0	40.0
64-65	36.76871835206637	37.0	37.0	40.0	33.0	40.0
66-67	36.559952158251875	37.0	37.0	40.0	33.0	40.0
68-69	36.34288334385443	37.0	37.0	40.0	33.0	40.0
70-71	36.105594496852945	37.0	37.0	37.0	33.0	40.0
72-73	35.880530734031154	37.0	37.0	37.0	33.0	40.0
74-75	35.686987443952276	37.0	35.0	37.0	33.0	40.0
76-77	34.004632520311354	35.0	33.0	37.0	30.0	37.0
78-79	35.19943846608603	37.0	33.0	37.0	33.0	37.0
80-81	35.26258734903638	37.0	33.0	37.0	33.0	37.0
82-83	35.12623010237717	37.0	33.0	37.0	33.0	37.0
84-85	34.9973069601319	37.0	33.0	37.0	33.0	37.0
86-87	34.91442954042229	37.0	33.0	37.0	33.0	37.0
88-89	34.77898560596017	37.0	33.0	37.0	33.0	37.0
90-91	34.631403932484346	37.0	33.0	37.0	33.0	37.0
92-93	34.63142726292213	37.0	33.0	37.0	33.0	37.0
94-95	34.69169202916659	37.0	33.0	37.0	33.0	37.0
96-97	34.698389924998025	37.0	33.0	37.0	33.0	37.0
98-99	34.699914720303575	37.0	33.0	37.0	33.0	37.0
100	34.69343736619862	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	13.0
20	72.0
21	371.0
22	1383.0
23	3867.0
24	8781.0
25	17570.0
26	30485.0
27	48145.0
28	71493.0
29	100279.0
30	136333.0
31	182500.0
32	242348.0
33	326558.0
34	451903.0
35	661748.0
36	1180721.0
37	2953672.0
38	3550590.0
39	92210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99205788107655	19.768189043621025	14.255092638009797	23.984660437292636
2	14.940001747333751	20.54801872927091	41.48280650425607	23.029173019139268
3	18.48547908999097	25.611489782918134	29.976762846555772	25.92626828053513
4	12.79499550891493	17.70997301174441	38.14801308373297	31.34701839560769
5	13.776802265928096	37.40840785592508	35.12769998100595	13.687089897140883
6	30.74112307410336	37.150766303403586	18.465250270648287	13.642860351844762
7	27.19449663419588	32.20299326819297	22.646926367375627	17.95558373023552
8	24.752384022213203	36.69831249106082	20.577478895577723	17.971824591148252
9	25.856364991184318	16.811249092166687	20.759070406517495	36.57331551013151
10-11	24.76923278015997	26.43052218008841	28.358281872947806	20.441963166803813
12-13	25.593526992532183	23.941218016980745	28.44127874627698	22.023976244210093
14-15	22.621975299732803	25.282134059190824	26.107575292655532	25.988315348420837
16-17	22.242147260477864	28.336172502194852	26.80787667839209	22.613803558935192
18-19	22.29440327409395	27.498610233551336	28.5050516134361	21.701934878918617
20-21	23.03596596077381	26.63340690100876	28.433804672379825	21.896822465837598
22-23	22.832044325757337	26.57529548765341	28.27939481504344	22.31326537154581
24-25	22.568728247298207	26.766314911613165	28.28065491701785	22.38430192407078
26-27	22.48981804257774	26.878711628068885	28.23893162796943	22.392538701383952
28-29	22.443647047310893	26.92347128407665	28.288435260165617	22.34444640844684
30-31	22.72509728538983	26.79414952249322	28.162368934718884	22.318384257398066
32-33	22.451979283459885	26.983742383918347	28.136593701246106	22.427684631375662
34-35	22.608286131394742	26.953896842198183	28.12987952815339	22.307937498253686
36-37	22.60354738218575	26.958417779412684	28.019076247726865	22.418958590674706
38-39	22.490575401097963	27.066245179654082	28.07452274816423	22.368656671083727
40-41	22.72728883794031	26.922048672996535	27.94017243753848	22.410490051524675
42-43	22.58772985061228	27.031131127170738	27.743116123069438	22.638022899147543
44-45	22.656457502894465	27.11819467418424	27.592497259359455	22.63285056356184
46-47	22.813605215165698	27.15441975451075	27.291246626107068	22.740728404216487
48-49	22.691921005329885	27.275686923140753	27.243391217828854	22.789000853700507
50-51	22.72952164328591	27.42629965113884	27.12854584117877	22.715632864396476
52-53	22.910892251282572	27.289914374159107	27.02411093970977	22.77508243484855
54-55	22.813646267573127	27.20278432561245	27.099941081438434	22.88362832537599
56-57	22.789380272006834	27.355620304487303	27.07130387573054	22.78369554777532
58-59	22.787534963395583	27.353191172152968	27.063869763785302	22.795404100666143
60-61	22.874006685061282	27.313893678456353	27.097008150514302	22.71509148596806
62-63	22.841917506783076	27.40203608111103	27.050664669300197	22.7053817428057
64-65	22.850350287281024	27.32119181647207	26.985371212512238	22.84308668373467
66-67	22.891566532281765	27.32999444849958	26.898777272605283	22.879661746613376
68-69	22.798549930682885	27.32244197486696	26.991905549841245	22.88710254460892
70-71	22.81832102363004	27.17613469134399	27.026966083238012	22.97857820178796
72-73	22.851242365878075	27.20779340368211	26.909061950838314	23.0319022796015
74-75	22.73049794951716	27.328990235479463	26.948738043625507	22.99177377137787
76-77	22.91625216204551	27.22198988453661	26.888631375134047	22.973126578283832
78-79	22.833389708230616	27.257899154819537	26.852320482285446	23.056390654664398
80-81	22.846821972274856	27.318759136523784	26.860821032854304	22.973597858347052
82-83	22.911650543211202	27.242896836609994	26.902723936914402	22.942728683264402
84-85	22.9263012653965	27.26745221471812	26.891172735910633	22.915073783974744
86-87	22.831964839127195	27.27369496824517	26.9686189161797	22.925721276447934
88-89	22.867552240174874	27.234344762225042	26.91438267170088	22.983720325899203
90-91	22.93688628555381	27.28684469267581	26.871620771880224	22.90464824989016
92-93	22.909129673269558	27.25208944240476	26.96606713128818	22.8727137530375
94-95	22.7898867050441	27.275377322508582	26.979753642709703	22.954982329737614
96-97	22.917879673819797	27.368777144261138	26.982208540189767	22.7311346417293
98-99	24.062035720969472	29.291669126037167	27.126315786529066	19.519979366464295
100	22.925120154824715	30.28062712095549	20.93912873305378	25.855123991166014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2023.0
1	1866.5
2	2507.0
3	3475.5
4	4659.0
5	7640.0
6	10200.5
7	11794.0
8	12715.0
9	12562.5
10	11752.0
11	10767.0
12	11529.0
13	15008.0
14	23482.5
15	35480.5
16	45702.5
17	52230.0
18	54150.0
19	51750.0
20	46683.0
21	40540.0
22	36197.0
23	36121.0
24	40590.0
25	49604.0
26	61928.0
27	76129.0
28	93161.0
29	113901.0
30	135634.0
31	157666.0
32	180936.0
33	203823.0
34	226208.5
35	247278.0
36	270042.5
37	292525.5
38	312284.5
39	329995.5
40	346029.0
41	359969.5
42	369883.5
43	380125.5
44	390159.0
45	399977.5
46	405993.5
47	409492.0
48	414186.0
49	414267.5
50	412085.5
51	406785.0
52	396538.0
53	382519.5
54	365238.5
55	344764.5
56	322138.0
57	296605.0
58	266624.5
59	233070.5
60	195316.0
61	159086.0
62	128196.0
63	100054.5
64	75197.0
65	56432.0
66	42934.5
67	33183.0
68	25492.0
69	18397.5
70	12986.5
71	9542.5
72	6935.5
73	4835.5
74	3271.5
75	2189.5
76	1392.0
77	856.5
78	547.0
79	345.0
80	233.5
81	163.5
82	123.0
83	87.5
84	57.0
85	38.0
86	25.0
87	19.5
88	15.5
89	13.0
90	9.0
91	5.5
92	5.0
93	3.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.4908991045958157E-4
7	0.0
8	0.0
9	0.0
10-11	0.006306503212440301
12-13	9.939327363972106E-6
14-15	0.006619592024405422
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	140.0
20-21	590.0
22-23	1233.0
24-25	3561.0
26-27	3886.0
28-29	6222.0
30-31	16067.0
32-33	19766.0
34-35	28612.0
36-37	41869.0
38-39	51278.0
40-41	53302.0
42-43	64392.0
44-45	77656.0
46-47	76083.0
48-49	68191.0
50-51	60337.0
52-53	58501.0
54-55	63350.0
56-57	66238.0
58-59	67065.0
60-61	66144.0
62-63	63655.0
64-65	67621.0
66-67	72300.0
68-69	74639.0
70-71	74277.0
72-73	70911.0
74-75	72991.0
76-77	72839.0
78-79	78400.0
80-81	80039.0
82-83	78357.0
84-85	77944.0
86-87	83068.0
88-89	89286.0
90-91	94059.0
92-93	97236.0
94-95	118996.0
96-97	342006.0
98-99	631119.0
100-101	6826817.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.78195121216804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1536996426231	74.69470913842532
2	7.483340399207354	12.539377204608732
3	1.8613568197900756	4.678443187922651
4	0.6754148090339803	2.2635028231384293
5	0.3090697901768907	1.2947235040877634
6	0.1668805776834669	0.8388948250640786
7	0.09688363377184751	0.5681969914551338
8	0.061124888153150814	0.4096929917677208
9	0.037445102023009415	0.28235013397437864
>10	0.14920019209957527	2.021540444991944
>50	0.004246412753750107	0.23678910463279
>100	0.001316042330329982	0.1606943630822344
>500	2.1690353505389692E-5	0.011085286848895747
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.939327363972104E-6	0.0	0.0	0.0
3	0.0	9.939327363972104E-6	0.0	1.987865472794421E-5	0.0
4	0.0	5.963596418383263E-5	0.0	1.987865472794421E-5	0.0
5	0.0	5.963596418383263E-5	0.0	2.9817982091916314E-5	0.0
6	1.0933260100369316E-4	5.963596418383263E-5	0.0	2.9817982091916314E-5	2.9817982091916314E-5
7	1.0933260100369316E-4	5.963596418383263E-5	0.0	2.9817982091916314E-5	2.9817982091916314E-5
8	1.0933260100369316E-4	5.963596418383263E-5	0.0	2.9817982091916314E-5	2.9817982091916314E-5
9	1.0933260100369316E-4	1.0933260100369316E-4	0.0	2.9817982091916314E-5	2.9817982091916314E-5
10-11	1.0933260100369316E-4	1.0933260100369316E-4	0.0	2.9817982091916314E-5	2.9817982091916314E-5
12-13	1.0933260100369316E-4	1.0933260100369316E-4	0.0	2.9817982091916314E-5	2.9817982091916314E-5
14-15	1.0933260100369316E-4	1.987865472794421E-4	0.0	2.9817982091916314E-5	6.460562786581869E-5
16-17	1.3915058309560947E-4	2.0872587464341422E-4	0.0	2.9817982091916314E-5	6.957529154780473E-5
18-19	1.3915058309560947E-4	2.484831840993026E-4	0.0	3.975730945588842E-5	8.945394627574895E-5
20-21	1.3915058309560947E-4	2.8824049355519107E-4	0.0	3.975730945588842E-5	8.945394627574895E-5
22-23	1.3915058309560947E-4	3.2302813932909345E-4	0.0	4.969663681986052E-5	1.0436293732170711E-4
24-25	1.3915058309560947E-4	3.6278544878498185E-4	0.0	6.460562786581869E-5	1.242415920496513E-4
26-27	1.3915058309560947E-4	3.677551124669679E-4	0.0	1.1927192836766527E-4	1.3915058309560947E-4
28-29	1.441202467775955E-4	3.72724776148954E-4	0.0	3.230281393290934E-4	1.3915058309560947E-4
30-31	1.4908991045958157E-4	4.075124219228563E-4	0.0	0.001222537265768569	1.3915058309560947E-4
32-33	1.4908991045958157E-4	4.075124219228563E-4	0.0	0.0035533095326200277	1.3915058309560947E-4
34-35	1.4908991045958157E-4	4.72118049788675E-4	0.0	0.006500320096037756	1.4908991045958157E-4
36-37	1.4908991045958157E-4	5.317540139725076E-4	0.0	0.010853745481457538	1.4908991045958157E-4
38-39	1.5405957414156763E-4	5.6654165974641E-4	0.0	0.01664340367097129	1.4908991045958157E-4
40-41	1.5902923782355367E-4	6.361169512942147E-4	0.0	0.022706393362994272	1.4908991045958157E-4
42-43	1.689685651875258E-4	6.559956060221589E-4	0.0	0.028207811058952835	1.4908991045958157E-4
44-45	1.7393822886951183E-4	7.255708975699637E-4	0.0	0.03442982998879937	1.4908991045958157E-4
46-47	1.789078925514979E-4	7.752675343898242E-4	0.0	0.041461904098809635	1.4908991045958157E-4
48-49	1.789078925514979E-4	7.752675343898242E-4	0.0	0.047897618566981576	1.4908991045958157E-4
50-51	1.789078925514979E-4	8.54782153301601E-4	0.0	0.05395066893164059	1.5902923782355367E-4
52-53	1.789078925514979E-4	8.647214806655731E-4	0.0	0.05971547880274441	1.6896856518752577E-4
54-55	1.789078925514979E-4	8.746608080295453E-4	0.0	0.06541568304598241	1.8387755623348396E-4
56-57	1.789078925514979E-4	8.846001353935174E-4	0.0	0.0700871669070493	1.8884721991547E-4
58-59	1.789078925514979E-4	8.846001353935174E-4	0.0	0.07502701260694344	1.9381688359745603E-4
60-61	1.789078925514979E-4	9.193877811674197E-4	0.0	0.07943510429286507	1.987865472794421E-4
62-63	1.789078925514979E-4	9.243574448494058E-4	0.0	0.08401216454397423	2.3854385673533052E-4
64-65	1.789078925514979E-4	0.0010535687005810432	0.0	0.08838049892043996	2.3854385673533052E-4
66-67	1.987865472794421E-4	0.0010535687005810432	0.0	0.09316131538251055	2.3854385673533052E-4
68-69	1.987865472794421E-4	0.0010585383642630294	0.0	0.09819061502868043	2.4351352041731655E-4
70-71	2.0375621096142816E-4	0.0010734473553089875	0.0	0.10258876738723809	2.484831840993026E-4
72-73	2.2860452937135842E-4	0.0010883563463549456	0.0	0.10595819936362463	2.7830116619121894E-4
74-75	2.2860452937135842E-4	0.0011430226468567921	0.0	0.10847284918670957	2.8824049355519107E-4
76-77	2.2860452937135842E-4	0.0012125979384045968	0.0	0.11110677093816218	2.8824049355519107E-4
78-79	2.2860452937135842E-4	0.0012622945752244572	0.0	0.11296542515522497	2.8824049355519107E-4
80-81	2.2860452937135842E-4	0.0012722339025884293	0.0	0.11349220950551549	2.9817982091916314E-4
82-83	2.2860452937135842E-4	0.0012772035662704153	0.0	0.11384505562693649	3.0811914828313526E-4
84-85	2.4351352041731655E-4	0.0012821732299524015	0.0	0.11399414553739606	3.0811914828313526E-4
86-87	2.484831840993026E-4	0.001331869866772262	0.0	0.11409353881103579	3.0811914828313526E-4
88	2.484831840993026E-4	0.0013815665035921225	0.0	0.11410347813839977	3.2799780301107946E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13260	0.0	48.794323	1
GTATCAA	23330	0.0	36.656536	1
TCAACGC	27970	0.0	29.946102	4
ATCAACG	28565	0.0	29.379173	3
CAACGCA	28745	0.0	29.139444	5
TATCAAC	29635	0.0	28.551603	2
AACGCAG	29665	0.0	28.279427	6
ACGCAGA	33960	0.0	24.677423	7
CGCAGAG	34835	0.0	24.045284	8
GTACATG	31430	0.0	22.179928	1
TACATGG	31520	0.0	21.719212	2
ACATGGG	31895	0.0	20.827385	3
GCAGAGT	40700	0.0	20.625402	9
GAGTACT	22695	0.0	20.12841	12-13
GTGGTAT	6840	0.0	19.764656	1
TGGTATC	6500	0.0	19.46952	2
CATGGGG	24415	0.0	19.214455	4
CAGAGTA	39725	0.0	18.623604	10-11
AGAGTAC	36900	0.0	18.30495	10-11
GTACTTT	25135	0.0	18.200705	14-15
>>END_MODULE