FastQCFastQC Report
Fri 27 May 2016
SRR2139120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8621658
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128900.043.7327041
GTATCAA215400.032.4265941
TCAACGC229650.029.4549774
ATCAACG234150.028.8862193
CAACGCA236500.028.5848465
AACGCAG243400.027.8635336
TATCAAC247850.027.516792
ACGCAGA279750.024.196547
CGCAGAG286300.023.689078
GTACATG267000.021.6484361
TACATGG268800.021.2455562
GTGGTAT61900.020.7196791
GCAGAGT332150.020.3818979
GAGTACT181050.020.29713212-13
ACATGGG275150.020.2828183
CTTATAC75100.018.8087371
CAGAGTA326750.018.74812710-11
GTACTTT201300.018.1907914-15
AGAGTAC305700.018.08976610-11
CATGGGG218750.017.1154354
Produced by FastQC (version 0.11.5)