##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139120_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8621658
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.623217367239576	33.0	33.0	33.0	33.0	33.0
2	32.625833105418934	33.0	33.0	33.0	33.0	33.0
3	32.62986075300134	33.0	33.0	33.0	33.0	33.0
4	36.547523689758975	37.0	37.0	37.0	37.0	37.0
5	36.57172843088882	37.0	37.0	37.0	37.0	37.0
6	36.578342935894696	37.0	37.0	37.0	37.0	37.0
7	36.551096204465544	37.0	37.0	37.0	37.0	37.0
8	36.54308254862348	37.0	37.0	37.0	37.0	37.0
9	36.57808660468787	37.0	37.0	37.0	37.0	37.0
10-11	36.52726697115567	37.0	37.0	37.0	37.0	37.0
12-13	36.51423519698879	37.0	37.0	37.0	37.0	37.0
14-15	38.61055994102294	40.0	37.0	40.0	37.0	40.0
16-17	38.549126977664855	40.0	37.0	40.0	37.0	40.0
18-19	38.56338572000884	40.0	37.0	40.0	37.0	40.0
20-21	38.560667581003194	40.0	37.0	40.0	37.0	40.0
22-23	38.55396339961016	40.0	37.0	40.0	37.0	40.0
24-25	38.54906001599529	40.0	37.0	40.0	37.0	40.0
26-27	38.42866769747275	40.0	37.0	40.0	37.0	40.0
28-29	38.38211804208013	40.0	37.0	40.0	37.0	40.0
30-31	38.22896038078868	40.0	37.0	40.0	37.0	40.0
32-33	38.24949611023902	40.0	37.0	40.0	37.0	40.0
34-35	38.22629277828534	40.0	37.0	40.0	37.0	40.0
36-37	38.17472158882867	40.0	37.0	40.0	37.0	40.0
38-39	38.118139680481534	40.0	37.0	40.0	37.0	40.0
40-41	38.09073594356117	40.0	37.0	40.0	33.0	40.0
42-43	38.04082096935794	40.0	37.0	40.0	33.0	40.0
44-45	37.964144728466295	40.0	37.0	40.0	33.0	40.0
46-47	37.814139672767936	40.0	37.0	40.0	33.0	40.0
48-49	37.82458296154425	40.0	37.0	40.0	33.0	40.0
50-51	37.78078273573617	40.0	37.0	40.0	33.0	40.0
52-53	37.71391114046024	40.0	37.0	40.0	33.0	40.0
54-55	37.5428878138597	40.0	37.0	40.0	33.0	40.0
56-57	37.41923169750111	40.0	37.0	40.0	33.0	40.0
58-59	37.28573778993656	38.5	37.0	40.0	33.0	40.0
60-61	37.116890388253026	37.0	37.0	40.0	33.0	40.0
62-63	36.94318663395006	37.0	37.0	40.0	33.0	40.0
64-65	36.7600496505093	37.0	37.0	40.0	33.0	40.0
66-67	36.551057653695565	37.0	37.0	40.0	33.0	40.0
68-69	36.33530319774091	37.0	37.0	40.0	33.0	40.0
70-71	36.10429479440457	37.0	37.0	38.5	33.0	40.0
72-73	35.87451954674304	37.0	37.0	37.0	33.0	40.0
74-75	35.678349748804116	37.0	35.0	37.0	33.0	40.0
76-77	34.008046291154976	35.0	33.0	37.0	30.0	37.0
78-79	35.20045705408829	37.0	33.0	37.0	33.0	37.0
80-81	35.25930243935366	37.0	33.0	37.0	33.0	37.0
82-83	35.119311010197606	37.0	33.0	37.0	33.0	37.0
84-85	34.99121572880777	37.0	33.0	37.0	33.0	37.0
86-87	34.90815632606654	37.0	33.0	37.0	33.0	37.0
88-89	34.770892335506595	37.0	33.0	37.0	33.0	37.0
90-91	34.622149003242285	37.0	33.0	37.0	33.0	37.0
92-93	34.61750028855828	37.0	33.0	37.0	33.0	37.0
94-95	34.677185982304294	37.0	33.0	37.0	33.0	37.0
96-97	34.6836299224957	37.0	33.0	37.0	33.0	37.0
98-99	34.6871077766952	37.0	33.0	37.0	33.0	37.0
100	34.675362454204645	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	62.0
21	321.0
22	1252.0
23	3431.0
24	7943.0
25	15201.0
26	27167.0
27	42007.0
28	62538.0
29	86644.0
30	117245.0
31	156133.0
32	206482.0
33	277145.0
34	385129.0
35	568438.0
36	1014650.0
37	2537471.0
38	3034890.0
39	77501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7594852405419	19.847470173370365	14.307340885013067	24.085703701074664
2	14.72484758732021	20.504524767741888	41.81586650734696	22.954761137590936
3	18.462353760726767	25.526215491266296	30.186641594922925	25.82478915308401
4	12.726809622928675	17.75904356215475	38.111764581708066	31.40238223320851
5	13.74504764628799	37.43595489405866	35.24464783919752	13.574349620455834
6	30.73426689819242	37.275395292801946	18.279859984988946	13.710477824016687
7	27.08434966917036	32.29483238606774	22.54188231544327	18.07893562931863
8	24.718459024934646	36.92285172991088	20.50436238598191	17.854326859172563
9	25.565453883696154	16.91973863959809	20.688816466623937	36.82599101008182
10-11	24.77411300303286	26.43727092096868	28.354063263264496	20.434552812733966
12-13	25.53079561914813	23.846890675823143	28.519061273201967	22.10325243182676
14-15	22.61206675412263	25.240559485416043	25.988961618067208	26.158412142394116
16-17	22.191897428545644	28.526589665236084	26.659779360304015	22.621733545914253
18-19	22.26132722963495	27.542556199747192	28.45340768562149	21.742708884996368
20-21	23.049976022055183	26.69293598552775	28.24386738471173	22.01322060770534
22-23	22.962362781881843	26.650512043179013	28.10877227374779	22.278352901191354
24-25	22.770990054899922	26.887938955741287	28.10262874565511	22.23844224370368
26-27	22.653278192169495	26.855182725509387	28.226503130935278	22.265035951385844
28-29	22.535816999792306	26.861487323068545	28.222647667667744	22.380048009471405
30-31	22.624606956624486	26.864546303903662	28.18990422193663	22.320942517535222
32-33	22.507304642273255	27.01448710415006	28.153656393816505	22.324551859760177
34-35	22.681022269466386	26.81488648167522	28.176455780711535	22.32763546814686
36-37	22.67745812800854	26.871448567341183	28.00762636205188	22.4434669425984
38-39	22.575797634906763	26.922521169872553	28.13401979716214	22.36766139805854
40-41	22.591742104445302	26.812574458954703	28.074752926354172	22.520930510245822
42-43	22.587315888944985	27.032152002433357	27.81236543583681	22.568166672784848
44-45	22.71523183551441	27.022900745133143	27.749106515909855	22.512760903442597
46-47	22.819242180106215	26.993470549636793	27.37681934164969	22.8104679286073
48-49	22.626811856471463	27.274406829034092	27.25365412117219	22.845127193322256
50-51	22.613991688455986	27.21785568700346	27.440118338476477	22.728034286064076
52-53	22.77616252421935	27.222736840091493	27.27371558727621	22.72738504841295
54-55	22.769670116111165	27.165661279343084	27.2381868171514	22.826481787394346
56-57	22.888459190738182	27.237210138410063	27.09891552942938	22.775415141422375
58-59	22.60594525223215	27.418675337880806	27.277751442346602	22.69762796754044
60-61	22.82140573766648	27.402598032060176	27.17661460191232	22.599381628361023
62-63	22.773544268404564	27.59497499098103	26.99291987032971	22.638560870284696
64-65	22.741063587039182	27.417898741019386	27.02590174734958	22.815135924591857
66-67	22.908716868435057	27.409661442274352	26.789074884330276	22.892546804960315
68-69	22.61858844491543	27.401833208660598	27.02252940198645	22.95704894443752
70-71	22.64629822284217	27.25754347449818	27.063354633181834	23.032803669477815
72-73	22.81783304023961	27.32652532403017	26.82854422961462	23.027097406115598
74-75	22.685702487005386	27.46890952332979	26.90899333786187	22.936394651802953
76-77	22.855076460598468	27.33790341058472	26.93950870708715	22.86751142172966
78-79	22.75331069425894	27.26142453131168	26.833095857373827	23.15216891705555
80-81	22.792696922388227	27.389207585690095	26.748187365530608	23.06990812639107
82-83	22.757573905593766	27.458436018226706	26.913462787875588	22.870527288303943
84-85	22.880187901657187	27.371269177165658	26.8132295333438	22.93531338783335
86-87	22.789752180772897	27.373886819237548	26.900585041224943	22.935775958764612
88-89	22.911923666158074	27.307911841558752	26.837641660053247	22.942522832229933
90-91	22.929952484630864	27.312446948851015	26.79552850231674	22.96207206420138
92-93	22.985455600755262	27.273458877714003	26.934494839586065	22.806590681944666
94-95	22.619972645591304	27.394223306316697	27.07035777535549	22.91544627273651
96-97	22.772556153336726	27.406180258880823	27.134057269194635	22.68720631858782
98-99	24.028318260858324	29.39064752386924	27.075573540534076	19.50546067473836
100	22.910875622759303	30.365628491969865	20.906184581085444	25.81731130418539
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1680.0
1	1477.0
2	1870.0
3	2613.5
4	3489.5
5	5608.5
6	7420.0
7	8703.5
8	9675.5
9	9757.0
10	9149.0
11	8343.0
12	8845.0
13	11581.5
14	18274.0
15	27756.0
16	35941.5
17	41224.0
18	42901.5
19	40795.0
20	36483.0
21	31771.5
22	29215.0
23	29790.5
24	33761.5
25	41586.5
26	53186.5
27	65770.5
28	77677.0
29	92085.0
30	108321.5
31	128076.5
32	150279.5
33	174568.0
34	196517.0
35	213851.5
36	236384.0
37	260062.5
38	275925.5
39	282657.0
40	290610.5
41	303676.0
42	314903.0
43	327473.5
44	343307.0
45	351308.0
46	354577.0
47	361329.5
48	366194.5
49	369617.0
50	364280.5
51	351126.0
52	337541.0
53	321409.0
54	303814.0
55	284231.5
56	263977.0
57	242300.0
58	219014.5
59	193370.5
60	163652.5
61	133924.5
62	106962.0
63	83287.5
64	63329.5
65	47924.0
66	36516.5
67	28046.5
68	21626.5
69	16142.0
70	11782.5
71	8607.0
72	6182.0
73	4383.5
74	2919.0
75	1845.0
76	1171.0
77	703.0
78	434.5
79	288.5
80	198.5
81	134.0
82	95.0
83	64.5
84	43.5
85	36.0
86	33.0
87	27.0
88	18.5
89	12.5
90	9.0
91	8.0
92	7.0
93	4.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.2758566855702232E-4
7	0.0
8	0.0
9	0.0
10-11	0.006825833267800695
12-13	1.7398045712321225E-5
14-15	0.006466273656412722
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	112.0
20-21	481.0
22-23	1010.0
24-25	2489.0
26-27	2839.0
28-29	4911.0
30-31	12340.0
32-33	15811.0
34-35	22565.0
36-37	33748.0
38-39	39706.0
40-41	42518.0
42-43	51407.0
44-45	62033.0
46-47	61181.0
48-49	54986.0
50-51	47949.0
52-53	45827.0
54-55	50613.0
56-57	54869.0
58-59	56642.0
60-61	57412.0
62-63	58035.0
64-65	60517.0
66-67	65820.0
68-69	62382.0
70-71	61476.0
72-73	58275.0
74-75	60155.0
76-77	60059.0
78-79	65273.0
80-81	66969.0
82-83	65315.0
84-85	64594.0
86-87	69203.0
88-89	74447.0
90-91	78663.0
92-93	82278.0
94-95	99259.0
96-97	294313.0
98-99	543188.0
100-101	5909988.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.6368349231256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.46414762133963	75.71962293765462
2	7.331431773095336	12.410183614592558
3	1.793035170856669	4.552704653014626
4	0.6326724708827889	2.1418958191405038
5	0.2966104223930084	1.2552083678277803
6	0.1552464530653121	0.7883741052293752
7	0.10063735003448243	0.5962338747378508
8	0.05676436304379478	0.38434848195632243
9	0.04207796964212628	0.3205211553450839
>10	0.12437906942164509	1.5879277482283058
>50	0.002506949951339585	0.14881437064019984
>100	4.4454905408499275E-4	0.062352837362281775
>500	4.5837219711737656E-5	0.03181203427051093
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1598697141547484E-5
2	0.0	3.479609142464245E-5	0.0	0.0	1.1598697141547484E-5
3	0.0	3.479609142464245E-5	0.0	1.1598697141547484E-5	1.1598697141547484E-5
4	0.0	8.119087999083239E-5	0.0	1.1598697141547484E-5	2.3197394283094968E-5
5	0.0	8.119087999083239E-5	0.0	1.1598697141547484E-5	2.3197394283094968E-5
6	4.6394788566189936E-5	9.278957713237987E-5	0.0	1.1598697141547484E-5	3.479609142464245E-5
7	4.6394788566189936E-5	9.278957713237987E-5	0.0	1.1598697141547484E-5	3.479609142464245E-5
8	4.6394788566189936E-5	1.0438827427392736E-4	0.0	1.1598697141547484E-5	3.479609142464245E-5
9	4.6394788566189936E-5	1.507830628401173E-4	0.0	3.479609142464245E-5	3.479609142464245E-5
10-11	4.6394788566189936E-5	1.507830628401173E-4	0.0	4.0595439995416194E-5	3.479609142464245E-5
12-13	4.6394788566189936E-5	1.507830628401173E-4	0.0	5.799348570773742E-5	3.479609142464245E-5
14-15	6.95921828492849E-5	1.913785028355335E-4	0.0	8.119087999083239E-5	4.0595439995416194E-5
16-17	8.119087999083239E-5	1.9717785140630723E-4	0.0	8.699022856160613E-5	5.219413713696368E-5
18-19	8.119087999083239E-5	2.1457589711862846E-4	0.0	9.278957713237987E-5	5.799348570773742E-5
20-21	8.119087999083239E-5	2.319739428309497E-4	0.0	9.278957713237987E-5	5.799348570773742E-5
22-23	8.119087999083239E-5	2.4357263997249717E-4	0.0	9.278957713237987E-5	5.799348570773742E-5
24-25	8.119087999083239E-5	2.667700342555921E-4	0.0	1.1598697141547484E-4	5.799348570773742E-5
26-27	8.119087999083239E-5	2.667700342555921E-4	0.0	1.6238175998166477E-4	5.799348570773742E-5
28-29	8.119087999083239E-5	2.7256938282636583E-4	0.0	4.291517942372569E-4	5.799348570773742E-5
30-31	9.278957713237987E-5	3.015661256802346E-4	0.0	0.001484633234118078	5.799348570773742E-5
32-33	1.0438827427392736E-4	3.0736547425100835E-4	0.0	0.004442301005212687	6.95921828492849E-5
34-35	1.2178631998624857E-4	3.82757005671067E-4	0.0	0.008293068456206452	6.95921828492849E-5
36-37	1.2758566855702232E-4	4.407504913788044E-4	0.0	0.014295394226957274	6.95921828492849E-5
38-39	1.2758566855702232E-4	4.407504913788044E-4	0.0	0.030458178693703694	7.539153142005864E-5
40-41	1.2758566855702232E-4	4.7554658280344686E-4	0.0	0.04810559639456819	8.119087999083239E-5
42-43	1.2758566855702232E-4	4.813459313742206E-4	0.0	0.05929833913616151	9.858892570315362E-5
44-45	1.3338501712779606E-4	5.62536811365053E-4	0.0	0.07237007081468552	1.2178631998624857E-4
46-47	1.391843656985698E-4	6.379283427851117E-4	0.0	0.0865784748130812	1.2758566855702232E-4
48-49	1.391843656985698E-4	6.379283427851117E-4	0.0	0.0999401739201439	1.391843656985698E-4
50-51	1.391843656985698E-4	7.133198742051703E-4	0.0	0.1130814977815172	1.391843656985698E-4
52-53	1.391843656985698E-4	7.307179199174916E-4	0.0	0.14405581849801974	1.4498371426934355E-4
54-55	1.391843656985698E-4	7.307179199174916E-4	0.0	0.18080049104244217	1.507830628401173E-4
56-57	1.391843656985698E-4	7.539153142005865E-4	0.0	0.21839186847819758	1.507830628401173E-4
58-59	1.391843656985698E-4	7.539153142005865E-4	0.0	0.2472378282692262	1.507830628401173E-4
60-61	1.5658241141089103E-4	7.771127084836814E-4	0.0	0.2725113893406581	1.6818110855243852E-4
62-63	1.7398045712321226E-4	7.945107541960028E-4	0.0	0.2912606832699697	1.7398045712321226E-4
64-65	1.7398045712321226E-4	8.467048913329663E-4	0.0	0.2959987510522918	1.79779805693986E-4
66-67	1.7398045712321226E-4	8.467048913329663E-4	0.0	0.3008528058060294	1.8557915426475975E-4
68-69	1.7398045712321226E-4	8.583035884745138E-4	0.0	0.3055676761940685	1.8557915426475975E-4
70-71	1.79779805693986E-4	8.699022856160613E-4	0.0	0.31002737524499346	1.8557915426475975E-4
72-73	2.0877654854785472E-4	8.699022856160613E-4	0.0	0.3133504019760468	2.5517133711404463E-4
74-75	2.0877654854785472E-4	9.046983770407038E-4	0.0	0.31579772707291337	2.5517133711404463E-4
76-77	2.1457589711862846E-4	9.4529381703612E-4	0.0	0.31833204239834145	2.609706856848184E-4
78-79	2.2617459426017595E-4	9.626918627484412E-4	0.0	0.31995006064958736	2.667700342555921E-4
80-81	2.319739428309497E-4	9.626918627484412E-4	0.0	0.3205067981123816	2.7256938282636583E-4
82-83	2.3777329140172343E-4	9.684912113192149E-4	0.0	0.32083156163234494	2.899674285386871E-4
84-85	2.4357263997249717E-4	9.742905598899887E-4	0.0	0.32104033818089284	2.899674285386871E-4
86-87	2.4357263997249717E-4	0.001009086651314631	0.0	0.3210925323180298	2.899674285386871E-4
88	2.4357263997249717E-4	0.001055481439880821	0.0	0.32109833166660057	2.899674285386871E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12890	0.0	43.732704	1
GTATCAA	21540	0.0	32.426594	1
TCAACGC	22965	0.0	29.454977	4
ATCAACG	23415	0.0	28.886219	3
CAACGCA	23650	0.0	28.584846	5
AACGCAG	24340	0.0	27.863533	6
TATCAAC	24785	0.0	27.51679	2
ACGCAGA	27975	0.0	24.19654	7
CGCAGAG	28630	0.0	23.68907	8
GTACATG	26700	0.0	21.648436	1
TACATGG	26880	0.0	21.245556	2
GTGGTAT	6190	0.0	20.719679	1
GCAGAGT	33215	0.0	20.381897	9
GAGTACT	18105	0.0	20.297132	12-13
ACATGGG	27515	0.0	20.282818	3
CTTATAC	7510	0.0	18.808737	1
CAGAGTA	32675	0.0	18.748127	10-11
GTACTTT	20130	0.0	18.19079	14-15
AGAGTAC	30570	0.0	18.089766	10-11
CATGGGG	21875	0.0	17.115435	4
>>END_MODULE