FastQCFastQC Report
Fri 27 May 2016
SRR2139119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10537175
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157550.049.0699921
GTATCAA275650.035.726451
TCAACGC333300.029.035534
ATCAACG336000.028.8248463
CTTATAC118950.028.6057871
CAACGCA340450.028.376465
TATCAAC344700.028.3459782
AACGCAG350000.027.6634186
ACGCAGA402550.024.0306347
TATACAC158050.023.7252853
CGCAGAG411050.023.565448
TTATACA150800.023.3029212
GTACATG378950.021.5432361
TACATGG377550.021.1009862
GCAGAGT470300.020.5265039
GTGGTAT74100.020.2995661
ACATGGG384500.020.2737563
GAGTACT254150.019.75667812-13
TGGTATC73300.019.0010342
TACACAT198150.018.8400365
Produced by FastQC (version 0.11.5)