##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139119_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10537175
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.600776773660876	33.0	33.0	33.0	33.0	33.0
2	32.60990464711842	33.0	33.0	33.0	33.0	33.0
3	32.61209413338964	33.0	33.0	33.0	33.0	33.0
4	36.526604996120874	37.0	37.0	37.0	37.0	37.0
5	36.55253927167386	37.0	37.0	37.0	37.0	37.0
6	36.560546066663974	37.0	37.0	37.0	37.0	37.0
7	36.5341026413626	37.0	37.0	37.0	37.0	37.0
8	36.52724909665067	37.0	37.0	37.0	37.0	37.0
9	36.563363804814855	37.0	37.0	37.0	37.0	37.0
10-11	36.511431004989475	37.0	37.0	37.0	37.0	37.0
12-13	36.49715872612916	37.0	37.0	37.0	37.0	37.0
14-15	38.57024904682707	40.0	37.0	40.0	37.0	40.0
16-17	38.50710726546726	40.0	37.0	40.0	37.0	40.0
18-19	38.52448829975776	40.0	37.0	40.0	37.0	40.0
20-21	38.5238046050619	40.0	37.0	40.0	37.0	40.0
22-23	38.51719426906163	40.0	37.0	40.0	37.0	40.0
24-25	38.512722100261264	40.0	37.0	40.0	37.0	40.0
26-27	38.384595088466284	40.0	37.0	40.0	37.0	40.0
28-29	38.32788455929928	40.0	37.0	40.0	37.0	40.0
30-31	38.174112445542576	40.0	37.0	40.0	37.0	40.0
32-33	38.18433973291212	40.0	37.0	40.0	37.0	40.0
34-35	38.15721723023526	40.0	37.0	40.0	37.0	40.0
36-37	38.105704648382726	40.0	37.0	40.0	35.0	40.0
38-39	38.04457635694125	40.0	37.0	40.0	33.0	40.0
40-41	38.02261354315506	40.0	37.0	40.0	33.0	40.0
42-43	37.97549839808802	40.0	37.0	40.0	33.0	40.0
44-45	37.89738118790912	40.0	37.0	40.0	33.0	40.0
46-47	37.74865315369668	40.0	37.0	40.0	33.0	40.0
48-49	37.759777187129615	40.0	37.0	40.0	33.0	40.0
50-51	37.706233403670325	40.0	37.0	40.0	33.0	40.0
52-53	37.63791525801733	40.0	37.0	40.0	33.0	40.0
54-55	37.46631093333044	40.0	37.0	40.0	33.0	40.0
56-57	37.33978284416546	40.0	37.0	40.0	33.0	40.0
58-59	37.20582223904944	37.0	37.0	40.0	33.0	40.0
60-61	37.02990301657045	37.0	37.0	40.0	33.0	40.0
62-63	36.85539688776992	37.0	37.0	40.0	33.0	40.0
64-65	36.679176334632515	37.0	37.0	40.0	33.0	40.0
66-67	36.476632790839844	37.0	37.0	40.0	33.0	40.0
68-69	36.26359129894763	37.0	37.0	40.0	33.0	40.0
70-71	36.0302563813091	37.0	37.0	37.0	33.0	40.0
72-73	35.80668464986546	37.0	37.0	37.0	33.0	40.0
74-75	35.61399346055002	37.0	33.0	37.0	33.0	40.0
76-77	33.9281461876208	35.0	33.0	37.0	27.0	37.0
78-79	35.132820536131916	37.0	33.0	37.0	33.0	37.0
80-81	35.204134235408546	37.0	33.0	37.0	33.0	37.0
82-83	35.06511341179237	37.0	33.0	37.0	33.0	37.0
84-85	34.9382126308666	37.0	33.0	37.0	33.0	37.0
86-87	34.85444988480535	37.0	33.0	37.0	33.0	37.0
88-89	34.71960227008015	37.0	33.0	37.0	33.0	37.0
90-91	34.57258485029981	37.0	33.0	37.0	33.0	37.0
92-93	34.57234425624682	37.0	33.0	37.0	33.0	37.0
94-95	34.63500504843883	37.0	33.0	37.0	33.0	37.0
96-97	34.6442396557087	37.0	33.0	37.0	33.0	37.0
98-99	34.65009207397662	37.0	33.0	37.0	33.0	37.0
100	34.64072690112293	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	83.0
21	428.0
22	1633.0
23	4516.0
24	10629.0
25	20221.0
26	34842.0
27	55369.0
28	80069.0
29	110708.0
30	148721.0
31	197531.0
32	260603.0
33	349577.0
34	481642.0
35	705922.0
36	1255662.0
37	3109293.0
38	3611035.0
39	98680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64442556947189	19.887417642774274	14.296573797056611	24.17158299069722
2	14.776465229058072	20.460816110579923	41.780629058547476	22.98208960181453
3	18.34029519297155	25.894853222044805	30.148023545210172	25.61682803977347
4	12.535418648736496	17.964141242790408	37.964587282644544	31.535852825828552
5	13.620975261395962	37.52939473815326	35.11857779717998	13.731052203270803
6	30.394655026329698	37.19538258535134	18.489977177893987	13.91998521042498
7	26.89789246168921	32.33873405348208	22.45457629772686	18.308797187101856
8	24.781689589477253	36.475924524362554	20.755544061857186	17.986841824303003
9	25.799467124727453	16.63743840260791	20.547385803120854	37.015708669543784
10-11	24.6852391370144	26.426188756769214	28.284710725800462	20.60386138041593
12-13	25.60038283514861	23.84032813356714	28.34072897639286	22.218560054891388
14-15	22.68204377331343	25.21362879358949	25.93339734924762	26.17093008384946
16-17	22.250346985790785	28.380272701174654	26.587168762025875	22.78221155100869
18-19	22.238806890841236	27.390277754711295	28.386469808084236	21.984445546363233
20-21	23.153246270590216	26.51196430988101	28.25279052079915	22.08199889872963
22-23	22.89116111937499	26.706953371713904	28.058714060333724	22.343171448577387
24-25	22.58275697714608	26.95191642565534	28.170515933991297	22.29481066320728
26-27	22.501110125019093	27.18063188035974	28.128538352878795	22.18971964174237
28-29	22.400451832858888	27.047555256406646	28.193329252677056	22.358663658057406
30-31	22.882597587894605	26.856273787830016	28.040705021375917	22.220423602899466
32-33	22.50584184531923	27.040764474265355	28.122138072048646	22.331255608366764
34-35	22.574106169246583	26.923546922976023	28.179624223624423	22.322722684152975
36-37	22.469118666528345	27.065523995303558	28.00819338511602	22.457163953052078
38-39	22.416936912296055	26.866338138960106	28.196359023498076	22.52036592524576
40-41	22.623474776709415	26.75165509965675	28.184324167539366	22.44054595609447
42-43	22.590926611487074	26.767615137997087	28.04690539489239	22.59455285562345
44-45	22.563740238809288	27.04738635020023	27.753606809407817	22.635266601582664
46-47	22.861426127479824	26.856661869034536	27.296388202178047	22.98552380130759
48-49	22.692980077408443	26.973940973132283	27.362634079595306	22.97044486986397
50-51	22.5772140678914	27.173528213214187	27.337249623018177	22.91200809587624
52-53	22.728059533347178	27.167063101539163	27.357894950296085	22.746982414817577
54-55	22.75969093253133	27.111157710221512	27.182561233012457	22.946590124234696
56-57	22.851698053388514	27.3281541784854	26.975644932271877	22.844502835854215
58-59	22.620661086135005	27.518207631081214	27.234183011581294	22.626948271202487
60-61	22.889570611970253	27.60773027460754	26.88535248299506	22.61734663042715
62-63	22.720318392815834	27.76269898564483	26.855844110678373	22.661138510860965
64-65	22.737675857687453	27.60804872072003	26.849042813287593	22.80523260830492
66-67	22.77107982779519	27.513522273007386	26.76308625919994	22.952311639997486
68-69	22.77868612227949	27.34218579313097	26.898550121879182	22.980577962710363
70-71	22.786816209654265	27.140075067324183	26.95120449053035	23.121904232491204
72-73	22.844202057563663	27.175938244200136	26.843874122948574	23.135985575287624
74-75	22.75898226344236	27.329555663227005	26.80978356462707	23.101678508703564
76-77	22.90014626206999	27.27641174876586	26.758553133189373	23.06488885597478
78-79	22.874875744668614	27.248952144981896	26.67446478916139	23.2017073211881
80-81	22.8306257205444	27.31949907138567	26.728318920777777	23.121556287292147
82-83	22.976841159896203	27.233021247146667	26.828692079223014	22.96144551373412
84-85	23.035826898066087	27.24954242354511	26.695651271282863	23.018979407105945
86-87	22.836688019111758	27.33748365520653	26.830384192035584	22.995444133646128
88-89	22.946130125497053	27.30240588912095	26.759018436968212	22.99244554841379
90-91	22.975145253194473	27.341866390499465	26.717399214506926	22.965589141799136
92-93	22.981856530199583	27.274986224443232	26.844240792339765	22.89891645301742
94-95	22.799949522095307	27.333284570176993	26.908278419650816	22.958487488076884
96-97	22.926514457654726	27.42074325565087	26.909041480486835	22.743700806207574
98-99	24.09877818404475	29.288092963874007	27.07993500366556	19.533193848415678
100	22.980341588464285	30.275323190038378	20.835214621563118	25.90912059993422
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2123.0
1	1909.0
2	2662.0
3	3838.5
4	5346.5
5	9074.0
6	12058.0
7	13542.0
8	14044.0
9	13249.5
10	11941.5
11	10831.5
12	12136.5
13	16908.5
14	27566.5
15	42401.5
16	54897.0
17	62326.0
18	64078.0
19	60402.5
20	53128.0
21	44933.5
22	39041.5
23	38191.0
24	42296.0
25	50668.5
26	62894.5
27	77643.0
28	95339.0
29	116547.0
30	136859.0
31	157414.0
32	181113.5
33	205543.5
34	230362.0
35	255286.0
36	281945.0
37	306955.0
38	326506.5
39	342892.0
40	359067.5
41	374128.5
42	385729.0
43	398026.5
44	410750.5
45	425394.0
46	444679.5
47	461619.5
48	464609.0
49	462446.5
50	462685.0
51	452644.0
52	434154.0
53	410812.0
54	385056.5
55	358741.0
56	333001.5
57	304470.0
58	273165.5
59	239085.0
60	198713.5
61	160499.5
62	127166.0
63	98482.0
64	74227.0
65	54538.5
66	40613.0
67	30938.0
68	23757.0
69	17879.0
70	12989.0
71	9410.0
72	6765.0
73	4573.0
74	2985.5
75	1877.0
76	1196.5
77	743.5
78	434.0
79	264.0
80	178.0
81	126.0
82	100.0
83	71.5
84	45.5
85	32.5
86	23.0
87	17.0
88	11.0
89	5.0
90	3.0
91	2.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.1827482223651026E-4
7	0.0
8	0.0
9	0.0
10-11	0.006491303409120566
12-13	2.372552415614242E-5
14-15	0.006951578577749728
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	154.0
20-21	603.0
22-23	1373.0
24-25	2709.0
26-27	3982.0
28-29	7087.0
30-31	17399.0
32-33	22162.0
34-35	31829.0
36-37	46118.0
38-39	54904.0
40-41	59805.0
42-43	72149.0
44-45	87746.0
46-47	84658.0
48-49	76528.0
50-51	65191.0
52-53	62399.0
54-55	70660.0
56-57	79520.0
58-59	80057.0
60-61	86370.0
62-63	86330.0
64-65	87076.0
66-67	96013.0
68-69	84121.0
70-71	81817.0
72-73	77690.0
74-75	79503.0
76-77	78442.0
78-79	85430.0
80-81	87734.0
82-83	84562.0
84-85	84852.0
86-87	89589.0
88-89	96727.0
90-91	102350.0
92-93	105248.0
94-95	128239.0
96-97	358057.0
98-99	648960.0
100-101	6981032.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.43174106416518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.97644893990113	71.64075409818777
2	7.972828274681526	12.984825752258415
3	2.1022592125531614	5.135718835391543
4	0.8311685397760366	2.7073400524688993
5	0.38725545513224235	1.5767442974007098
6	0.23601667614096397	1.1531549315001544
7	0.13593413759621747	0.7748547446161048
8	0.08861402275716158	0.5772795324652178
9	0.05464297662503817	0.4004705451154805
>10	0.20896174686824498	2.643131319689355
>50	0.004954066867428735	0.2668624205132114
>100	8.479205013808958E-4	0.09930906488311197
>500	6.803059947719057E-5	0.03955440551007907
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.8470628987370902E-5	0.0	0.0	0.0
3	0.0	3.796083864982787E-5	0.0	1.8980419324913935E-5	0.0
4	0.0	1.0439230628702665E-4	0.0	4.745104831228484E-5	0.0
5	0.0	1.0439230628702665E-4	0.0	5.6941257974741804E-5	0.0
6	0.0	1.1388251594948361E-4	0.0	5.6941257974741804E-5	0.0
7	0.0	1.1388251594948361E-4	0.0	6.643146763719877E-5	0.0
8	1.8980419324913935E-5	1.3286293527439754E-4	0.0	7.592167729965574E-5	0.0
9	1.8980419324913935E-5	1.8980419324913936E-4	0.0	7.592167729965574E-5	0.0
10-11	1.8980419324913935E-5	1.8980419324913936E-4	0.0	9.01569917933412E-5	0.0
12-13	1.8980419324913935E-5	1.9454929808036784E-4	0.0	1.0913741111825513E-4	0.0
14-15	1.8980419324913935E-5	2.94196499536166E-4	0.0	1.2811783044316906E-4	0.0
16-17	1.8980419324913935E-5	2.94196499536166E-4	0.0	1.4235314493685452E-4	0.0
18-19	2.8470628987370902E-5	3.2266712852353687E-4	0.0	1.5658845943053998E-4	0.0
20-21	6.168636280597029E-5	3.511377575109078E-4	0.0	1.6133356426176846E-4	0.0
22-23	1.1388251594948361E-4	3.9858880582319264E-4	0.0	1.8505908841791086E-4	0.0
24-25	1.2337272561194058E-4	4.555300637979345E-4	0.0	2.0403950774282482E-4	0.0
26-27	1.2337272561194058E-4	4.745104831228484E-4	0.0	2.7521608021125203E-4	0.0
28-29	1.2337272561194058E-4	4.745104831228484E-4	0.0	6.310989425533883E-4	0.0
30-31	1.2337272561194058E-4	5.314517410975901E-4	0.0	0.0019170223518163074	0.0
32-33	1.47098249768083E-4	5.314517410975901E-4	0.0	0.004488869170342146	0.0
34-35	1.6133356426176846E-4	5.97883208734789E-4	0.0	0.008280207930493704	0.0
36-37	1.6133356426176846E-4	6.595695715407592E-4	0.0	0.014434608896597048	0.0
38-39	1.7082377392422542E-4	6.738048860344447E-4	0.0	0.03265581144851443	0.0
40-41	1.8031398358668238E-4	7.402363536716435E-4	0.0	0.053036036698640765	0.0
42-43	1.8980419324913936E-4	7.829422971526998E-4	0.0	0.06443377850325158	0.0
44-45	1.8980419324913936E-4	9.300405469207828E-4	0.0	0.07941407445543991	0.0
46-47	1.8980419324913936E-4	0.0010249426435453525	0.0	0.09438962530279701	0.0
48-49	1.8980419324913936E-4	0.0010344328532078096	0.0	0.10864392021580738	0.0
50-51	1.8980419324913936E-4	0.0011530604739885216	0.0	0.12300260743510476	0.0
52-53	1.9454929808036784E-4	0.001167295788482207	0.0	0.16094446566560772	0.0
54-55	1.9929440291159632E-4	0.0011720408933134354	0.0	0.20561488254679267	0.0
56-57	1.9929440291159632E-4	0.001176785998144664	0.0	0.2526293812146045	0.0
58-59	1.9929440291159632E-4	0.001176785998144664	0.0	0.2874489604661591	0.0
60-61	1.9929440291159632E-4	0.001186276207807121	0.0	0.310728444768166	0.0
62-63	2.0403950774282482E-4	0.0012100017319632635	0.0	0.3270421151779296	0.0
64-65	2.087846125740533E-4	0.0013570999817313465	0.0	0.33221427944396864	0.0
66-67	2.1827482223651026E-4	0.0013665901913938034	0.0	0.33664620735633605	0.0
68-69	2.1827482223651026E-4	0.0013998059252124028	0.0	0.34160958700980104	0.0
70-71	2.1827482223651026E-4	0.0014235314493685452	0.0	0.34584696562408807	0.0
72-73	2.1827482223651026E-4	0.0014235314493685452	0.0	0.34941528445717185	0.0
74-75	2.2301992706773874E-4	0.0014804727073432868	0.0	0.3522860728800651	0.0
76-77	2.2776503189896722E-4	0.0015611394894741713	0.0	0.35498129242420284	0.0
78-79	2.2776503189896722E-4	0.0015991003281239991	0.0	0.357059648340281	0.0
80-81	2.2776503189896722E-4	0.0016038454329552277	0.0	0.3576148256055347	0.0
82-83	2.2776503189896722E-4	0.0016038454329552277	0.0	0.35809882629832	0.0
84-85	2.2776503189896722E-4	0.001608590537786456	0.0	0.3582838853867379	0.0
86-87	2.372552415614242E-4	0.0016418062716050555	0.0	0.35835506195920636	0.0
88	2.4674545122388116E-4	0.001746198577892082	0.0	0.3583598070640376	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15755	0.0	49.069992	1
GTATCAA	27565	0.0	35.72645	1
TCAACGC	33330	0.0	29.03553	4
ATCAACG	33600	0.0	28.824846	3
CTTATAC	11895	0.0	28.605787	1
CAACGCA	34045	0.0	28.37646	5
TATCAAC	34470	0.0	28.345978	2
AACGCAG	35000	0.0	27.663418	6
ACGCAGA	40255	0.0	24.030634	7
TATACAC	15805	0.0	23.725285	3
CGCAGAG	41105	0.0	23.56544	8
TTATACA	15080	0.0	23.302921	2
GTACATG	37895	0.0	21.543236	1
TACATGG	37755	0.0	21.100986	2
GCAGAGT	47030	0.0	20.526503	9
GTGGTAT	7410	0.0	20.299566	1
ACATGGG	38450	0.0	20.273756	3
GAGTACT	25415	0.0	19.756678	12-13
TGGTATC	7330	0.0	19.001034	2
TACACAT	19815	0.0	18.840036	5
>>END_MODULE