FastQCFastQC Report
Fri 27 May 2016
SRR2139118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7280606
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96850.050.819581
GTATCAA178200.037.278471
TCAACGC214950.030.5069584
ATCAACG216000.030.3752943
CAACGCA220200.029.839215
TATCAAC224250.029.3732242
AACGCAG226100.029.0605626
ACGCAGA262050.025.0738147
CGCAGAG265650.024.7017148
GTACATG242850.022.8604871
TACATGG246350.022.4131262
ACATGGG248350.021.6594473
GCAGAGT309200.021.0300889
GTGGTAT48050.020.9173931
GAGTACT171850.020.13411312-13
TGGTATC48000.019.5911672
CATGGGG179550.019.00574
CAGAGTA302350.018.80827310-11
AGAGTAC279250.018.68771210-11
GTACTTT192000.017.9878714-15
Produced by FastQC (version 0.11.5)