##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139118_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7280606
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59647109045593	33.0	33.0	33.0	33.0	33.0
2	32.60569271294175	33.0	33.0	33.0	33.0	33.0
3	32.60796051867111	33.0	33.0	33.0	33.0	33.0
4	36.52078837942886	37.0	37.0	37.0	37.0	37.0
5	36.54855076624116	37.0	37.0	37.0	37.0	37.0
6	36.556673716446134	37.0	37.0	37.0	37.0	37.0
7	36.53093149114236	37.0	37.0	37.0	37.0	37.0
8	36.52300921104644	37.0	37.0	37.0	37.0	37.0
9	36.55973582418826	37.0	37.0	37.0	37.0	37.0
10-11	36.50841784323997	37.0	37.0	37.0	37.0	37.0
12-13	36.4939978210605	37.0	37.0	37.0	37.0	37.0
14-15	38.55812043667794	40.0	37.0	40.0	37.0	40.0
16-17	38.495469401860234	40.0	37.0	40.0	37.0	40.0
18-19	38.510003425539026	40.0	37.0	40.0	37.0	40.0
20-21	38.50943676216396	40.0	37.0	40.0	37.0	40.0
22-23	38.50652838715492	40.0	37.0	40.0	37.0	40.0
24-25	38.5047239888446	40.0	37.0	40.0	37.0	40.0
26-27	38.37884855232478	40.0	37.0	40.0	37.0	40.0
28-29	38.320687010635694	40.0	37.0	40.0	37.0	40.0
30-31	38.16792903877542	40.0	37.0	40.0	37.0	40.0
32-33	38.17943970313715	40.0	37.0	40.0	37.0	40.0
34-35	38.15338245186493	40.0	37.0	40.0	37.0	40.0
36-37	38.10486024048076	40.0	37.0	40.0	35.0	40.0
38-39	38.0460310457803	40.0	37.0	40.0	33.0	40.0
40-41	38.02369364840626	40.0	37.0	40.0	33.0	40.0
42-43	37.97186948328627	40.0	37.0	40.0	33.0	40.0
44-45	37.89080685433686	40.0	37.0	40.0	33.0	40.0
46-47	37.74039786760279	40.0	37.0	40.0	33.0	40.0
48-49	37.751594378003716	40.0	37.0	40.0	33.0	40.0
50-51	37.70798691866016	40.0	37.0	40.0	33.0	40.0
52-53	37.64357649391913	40.0	37.0	40.0	33.0	40.0
54-55	37.473478648602224	40.0	37.0	40.0	33.0	40.0
56-57	37.35619161604352	40.0	37.0	40.0	33.0	40.0
58-59	37.22169336950135	37.0	37.0	40.0	33.0	40.0
60-61	37.05080113534715	37.0	37.0	40.0	33.0	40.0
62-63	36.879046083291755	37.0	37.0	40.0	33.0	40.0
64-65	36.7075828879631	37.0	37.0	40.0	33.0	40.0
66-67	36.507273382812784	37.0	37.0	40.0	33.0	40.0
68-69	36.29140950053548	37.0	37.0	40.0	33.0	40.0
70-71	36.05393221565694	37.0	37.0	38.5	33.0	40.0
72-73	35.82974972231071	37.0	37.0	37.0	33.0	40.0
74-75	35.63575239949584	37.0	33.0	37.0	33.0	40.0
76-77	33.95185744357011	35.0	33.0	37.0	27.0	37.0
78-79	35.150660605156204	37.0	33.0	37.0	33.0	37.0
80-81	35.206999995888395	37.0	33.0	37.0	33.0	37.0
82-83	35.06825836455973	37.0	33.0	37.0	33.0	37.0
84-85	34.940794793932696	37.0	33.0	37.0	33.0	37.0
86-87	34.850464587161824	37.0	33.0	37.0	33.0	37.0
88-89	34.71227226170993	37.0	33.0	37.0	33.0	37.0
90-91	34.565150090641794	37.0	33.0	37.0	33.0	37.0
92-93	34.560370335074666	37.0	33.0	37.0	33.0	37.0
94-95	34.61578761171327	37.0	33.0	37.0	33.0	37.0
96-97	34.626758049842394	37.0	33.0	37.0	33.0	37.0
98-99	34.638695738151796	37.0	33.0	37.0	33.0	37.0
100	34.63003129412584	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	65.0
21	300.0
22	1081.0
23	3070.0
24	7103.0
25	13811.0
26	24036.0
27	37505.0
28	54793.0
29	76638.0
30	103797.0
31	136343.0
32	181398.0
33	242731.0
34	335281.0
35	494293.0
36	878782.0
37	2137470.0
38	2490027.0
39	62073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80066879048255	19.960962040797153	14.233609125394231	24.004760043326065
2	14.742756303527482	20.754426211224725	41.67595389724427	22.826863588003526
3	18.290620313748608	26.035140481437946	30.22406376612057	25.45017543869288
4	12.53714319934357	17.918137583602245	38.30807490475381	31.236644312300378
5	13.57054618805083	37.608929806117786	35.24055552518568	13.579968480645704
6	30.30278848126952	37.42395194677797	18.61441525238188	13.65884431957063
7	26.69185779315623	32.474494568171934	22.7945860550619	18.039061583609936
8	24.626150625373768	36.680573018235016	20.86566695134993	17.827609405041283
9	25.79215600901299	16.61860518459661	20.777819975125695	36.81141883126471
10-11	24.543618167939833	26.496107288002445	28.5389723800755	20.42130216398222
12-13	25.38965511794693	24.02266707231061	28.660097615514097	21.927580194228362
14-15	22.47167088856409	25.386486819307898	26.202986648740172	25.938855643387843
16-17	22.071747873734687	28.5413398280308	26.851171454683854	22.535740843550663
18-19	22.11388722312401	27.529638329556633	28.646324220813487	21.71015022650587
20-21	22.921193073042705	26.72459688485262	28.526441996663383	21.8277680454413
22-23	22.666490747448112	26.755287003253432	28.41482520981011	22.16339703948835
24-25	22.445679842369408	26.90199465235903	28.467261241322344	22.185064263949215
26-27	22.425516168699115	27.022381826546425	28.411860241309917	22.14024176344454
28-29	22.23010424736357	26.981123034822424	28.450298454077384	22.338474263736625
30-31	22.633760910553775	26.952288772841747	28.314050283928804	22.09990003267567
32-33	22.450502743395827	26.98179592663785	28.40969193053602	22.158009399430302
34-35	22.325501880356878	27.04955100414062	28.49363192942333	22.131315186079174
36-37	22.481379158377457	27.080638690190355	28.228835574625073	22.209146576807118
38-39	22.249808185068837	27.092738482473848	28.357881839775235	22.29957149268208
40-41	22.473693599784525	26.968311989953865	28.27009839758501	22.287896012676605
42-43	22.427588537999878	27.041298149711967	28.09432167151987	22.43679164076828
44-45	22.462340470479166	27.195613269919438	27.88347587002944	22.458570389571957
46-47	22.68926381694193	27.200626523569483	27.488285731333	22.62182392815558
48-49	22.519172400759874	27.32198995846793	27.462847678127073	22.695989962645122
50-51	22.480940862457253	27.48436066394195	27.406985388780296	22.627713084820506
52-53	22.64180243427552	27.398198648669066	27.392988254940626	22.567010662114797
54-55	22.584063916617573	27.342301981831906	27.337309092660604	22.736325008889914
56-57	22.627637820692442	27.487705954920923	27.24108571755569	22.643570506830947
58-59	22.506727499244402	27.499207217091033	27.3094822190544	22.68458306461017
60-61	22.766865869033893	27.534272873936672	27.26965518867342	22.429206068356013
62-63	22.477545125896224	27.661227180541154	27.265559375218317	22.595668318344305
64-65	22.490309579316637	27.561903542095568	27.198853121485218	22.74893375710258
66-67	22.56035712488138	27.52427144240838	27.244882081638718	22.67048935107152
68-69	22.553748503405647	27.607402949543037	27.17408150174544	22.664767045305876
70-71	22.533019001636863	27.44589253701179	27.088821662992096	22.932266798359247
72-73	22.571547078515625	27.461282040093426	26.964379303603657	23.00279157778729
74-75	22.464365762886	27.609884228793398	27.15556721631916	22.77018279200145
76-77	22.63466067298768	27.337993113209	27.079009325114995	22.94833688868833
78-79	22.57967448591753	27.376737312995786	26.860081914934813	23.18350628615187
80-81	22.741778142050006	27.626092459573005	26.890065563287497	22.74206383508949
82-83	22.759721069261975	27.377131075848617	26.964456033126115	22.898691821763293
84-85	22.670958675060902	27.393865933918853	26.919475982765377	23.015699408254868
86-87	22.621804888234767	27.56888381372024	26.965344488840966	22.843966809204034
88-89	22.80665694559944	27.54196830453989	26.900763628605155	22.750611121255517
90-91	22.835452690954277	27.571707910198867	26.91062580050192	22.682213598344937
92-93	22.67186387757725	27.364859015154753	26.994480298196326	22.968796809071673
94-95	22.69684907973426	27.564624669856713	27.04732927492849	22.691196975480537
96-97	22.958268491929715	27.487474976908672	27.071426774438173	22.48282975672344
98-99	23.819082285173028	29.516130325889062	27.320133656944694	19.344653731993215
100	22.70109088822443	30.445065180725113	21.187455297765553	25.666388633284896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1609.0
1	1383.5
2	1840.5
3	2722.0
4	3687.5
5	6044.0
6	8001.0
7	9177.0
8	9852.5
9	9516.0
10	8673.5
11	7791.0
12	8345.0
13	11239.0
14	18187.5
15	28100.5
16	36596.0
17	41930.5
18	43553.0
19	41165.5
20	36166.0
21	30906.0
22	27317.0
23	26991.0
24	30133.0
25	36468.5
26	46089.5
27	57282.0
28	70046.0
29	85705.5
30	102236.0
31	118875.0
32	136349.0
33	153586.0
34	170295.0
35	184757.5
36	199943.5
37	216416.0
38	231229.5
39	242817.5
40	252864.0
41	263167.5
42	270728.5
43	277187.0
44	283267.5
45	290152.5
46	295607.0
47	298348.0
48	299986.0
49	300009.5
50	300181.0
51	295651.0
52	284785.5
53	272170.5
54	258621.0
55	242525.0
56	224080.0
57	204148.0
58	182771.5
59	159611.5
60	133948.5
61	109609.0
62	87588.5
63	67895.0
64	51086.0
65	37662.5
66	28460.0
67	21768.0
68	16534.0
69	12000.5
70	8468.5
71	6032.0
72	4229.5
73	2906.0
74	1905.5
75	1262.0
76	847.5
77	519.0
78	322.0
79	227.0
80	155.5
81	102.0
82	77.0
83	52.0
84	31.0
85	20.0
86	14.5
87	11.5
88	10.0
89	9.5
90	9.0
91	7.5
92	5.5
93	3.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.6482144480830304E-4
7	0.0
8	0.0
9	1.373512040069192E-5
10-11	0.0063593607455203595
12-13	1.373512040069192E-5
14-15	0.006956838482950457
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	101.0
20-21	456.0
22-23	923.0
24-25	2137.0
26-27	2777.0
28-29	4729.0
30-31	11470.0
32-33	14752.0
34-35	20688.0
36-37	30050.0
38-39	35952.0
40-41	39207.0
42-43	47715.0
44-45	58090.0
46-47	56151.0
48-49	50293.0
50-51	43480.0
52-53	41022.0
54-55	45272.0
56-57	49590.0
58-59	31745.0
60-61	53925.0
62-63	52858.0
64-65	54806.0
66-67	59604.0
68-69	55966.0
70-71	55467.0
72-73	53410.0
74-75	54445.0
76-77	54444.0
78-79	58491.0
80-81	60672.0
82-83	58438.0
84-85	58349.0
86-87	62305.0
88-89	66307.0
90-91	69933.0
92-93	73791.0
94-95	88501.0
96-97	268990.0
98-99	453794.0
100-101	4879510.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.497022149066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65149866320277	76.64936166904808
2	7.338934243212648	12.549140470849816
3	1.7594296067340691	4.512779761699956
4	0.6107525181108141	2.0887008627407244
5	0.2567711655097404	1.0976585012413882
6	0.13071266713586574	0.6705326278367162
7	0.07838733284349925	0.4691318472628805
8	0.044599390257377865	0.30504920453358986
9	0.02994522588141558	0.2304204876397955
>10	0.09679246352601355	1.2688763650963124
>50	0.0018337942051969737	0.10767367717319891
>100	3.429293806442521E-4	0.05067452487753566
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.373512040069192E-5	0.0	0.0	0.0
3	0.0	1.373512040069192E-5	0.0	0.0	0.0
4	0.0	5.494048160276768E-5	0.0	1.373512040069192E-5	0.0
5	0.0	6.86756020034596E-5	0.0	1.373512040069192E-5	0.0
6	0.0	6.86756020034596E-5	0.0	1.373512040069192E-5	0.0
7	0.0	6.86756020034596E-5	0.0	1.373512040069192E-5	0.0
8	0.0	6.86756020034596E-5	0.0	1.373512040069192E-5	1.373512040069192E-5
9	0.0	1.6482144480830304E-4	0.0	1.373512040069192E-5	1.373512040069192E-5
10-11	0.0	1.6482144480830304E-4	0.0	2.747024080138384E-5	1.373512040069192E-5
12-13	0.0	1.6482144480830304E-4	0.0	5.494048160276768E-5	1.373512040069192E-5
14-15	0.0	2.3349704681176263E-4	0.0	5.494048160276768E-5	4.120536120207576E-5
16-17	0.0	2.4723216721245456E-4	0.0	5.494048160276768E-5	6.180804180311364E-5
18-19	0.0	2.678348478134925E-4	0.0	5.494048160276768E-5	6.86756020034596E-5
20-21	0.0	2.8843752841453034E-4	0.0	5.494048160276768E-5	8.241072240415152E-5
22-23	0.0	3.0217264881522227E-4	0.0	6.86756020034596E-5	9.614584280484345E-5
24-25	0.0	3.159077692159142E-4	0.0	7.554316220380555E-5	1.2361608360622728E-4
26-27	0.0	3.296428896166061E-4	0.0	1.1674852340588131E-4	1.373512040069192E-4
28-29	0.0	3.296428896166061E-4	0.0	2.8843752841453034E-4	1.373512040069192E-4
30-31	0.0	3.5711313041798993E-4	0.0	0.0012911013176650406	1.5108632440761114E-4
32-33	0.0	3.7084825081868186E-4	0.0	0.003667277146984743	1.6482144480830304E-4
34-35	0.0	4.395238528221415E-4	0.0	0.006778281917741463	1.6482144480830304E-4
36-37	0.0	5.28802135426639E-4	0.0	0.01191521694760024	1.8542412540934093E-4
38-39	0.0	5.494048160276768E-4	0.0	0.02778614857059976	2.1976192641107073E-4
40-41	0.0	6.318155384318283E-4	0.0	0.04461167106144735	2.1976192641107073E-4
42-43	0.0	6.592857792332121E-4	0.0	0.05444601726834277	2.266294866114167E-4
44-45	0.0	7.622991822384016E-4	0.0	0.0669106390319707	2.4036460701210862E-4
46-47	0.0	8.378423444422072E-4	0.0	0.08026804362164358	2.4723216721245456E-4
48-49	0.0	8.378423444422072E-4	0.0	0.09266398978326804	2.540997274128005E-4
50-51	0.0	9.202530668463586E-4	0.0	0.10481270377768004	2.609672876131465E-4
52-53	0.0	9.202530668463586E-4	0.0	0.13879339164899185	2.747024080138384E-4
54-55	0.0	9.339881872470506E-4	0.0	0.1802185147774787	2.8843752841453034E-4
56-57	0.0	9.614584280484345E-4	0.0	0.22251581805140946	2.8843752841453034E-4
58-59	0.0	9.683259882487805E-4	0.0	0.39104574536789927	2.9530508861487633E-4
60-61	0.0	9.820611086494722E-4	0.0	0.5376612880850853	3.0217264881522227E-4
62-63	0.0	0.0010163989096512022	0.0	0.543491846695179	3.1590776921591415E-4
64-65	0.0	0.0010988096320553537	0.0	0.5485326358822329	3.1590776921591415E-4
66-67	0.0	0.0010988096320553537	0.0	0.5535734250692868	3.1590776921591415E-4
68-69	0.0	0.0011125447524560454	0.0	0.5582502335657225	3.1590776921591415E-4
70-71	0.0	0.0011262798728567374	0.0	0.5623432994451286	3.1590776921591415E-4
72-73	0.0	0.0011262798728567374	0.0	0.5656328607810943	3.5711313041798993E-4
74-75	0.0	0.0011606176738584674	0.0	0.5687095277508493	3.5711313041798993E-4
76-77	0.0	0.001201823035060543	0.0	0.5715595652339929	3.5711313041798993E-4
78-79	0.0	0.001208690595260889	0.0	0.57329019040448	3.5711313041798993E-4
80-81	0.0	0.001208690595260889	0.0	0.5737915222991053	3.639806906183359E-4
82-83	0.0	0.001208690595260889	0.0	0.5740868273877202	3.983184916200657E-4
84-85	0.0	0.0012361608360622728	0.0	0.5742516488325284	3.983184916200657E-4
86-87	0.0	0.0012567635166633108	0.0	0.5743752649161347	3.983184916200657E-4
88	0.0	0.0012911013176650406	0.0	0.5743890000365354	3.983184916200657E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9685	0.0	50.81958	1
GTATCAA	17820	0.0	37.27847	1
TCAACGC	21495	0.0	30.506958	4
ATCAACG	21600	0.0	30.375294	3
CAACGCA	22020	0.0	29.83921	5
TATCAAC	22425	0.0	29.373224	2
AACGCAG	22610	0.0	29.060562	6
ACGCAGA	26205	0.0	25.073814	7
CGCAGAG	26565	0.0	24.701714	8
GTACATG	24285	0.0	22.860487	1
TACATGG	24635	0.0	22.413126	2
ACATGGG	24835	0.0	21.659447	3
GCAGAGT	30920	0.0	21.030088	9
GTGGTAT	4805	0.0	20.917393	1
GAGTACT	17185	0.0	20.134113	12-13
TGGTATC	4800	0.0	19.591167	2
CATGGGG	17955	0.0	19.0057	4
CAGAGTA	30235	0.0	18.808273	10-11
AGAGTAC	27925	0.0	18.687712	10-11
GTACTTT	19200	0.0	17.98787	14-15
>>END_MODULE