FastQCFastQC Report
Fri 27 May 2016
SRR2139117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5556711
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68900.047.284831
GTATCAA120400.036.7932171
TCAACGC138150.031.498954
ATCAACG140350.031.0335673
CAACGCA143550.030.3458675
AACGCAG146950.029.613996
TATCAAC149750.029.2607632
ACGCAGA169500.025.6997647
ATCTCGT59750.025.33544294
CGCAGAG172300.025.333128
TATCTCG65900.023.92818694
GTACATG169400.022.6139011
TACATGG168750.022.1308882
GCAGAGT205300.021.2648939
ACATGGG171050.021.1174623
GTGGTAT37750.020.619961
TGGTATC36400.019.8229062
GAGTACT115000.019.60621512-13
CGCTATC94800.019.22846894
CATGGGG127800.019.1295554
Produced by FastQC (version 0.11.5)