FastQCFastQC Report
Fri 27 May 2016
SRR2139116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8593178
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113400.045.4450071
GTATCAA196100.033.5369451
TCAACGC217350.029.2085364
ATCAACG219050.028.999523
CAACGCA222250.028.4693665
TATCAAC230850.027.7413672
AACGCAG229250.027.7129546
ACGCAGA266450.023.8600437
CGCAGAG273950.023.2385778
GTACATG261200.022.81741
TACATGG259450.022.4892752
ACATGGG263250.021.7059523
GCAGAGT322200.019.7741349
GAGTACT171300.019.31178312-13
CATGGGG211000.019.0704464
CAGAGTA312250.018.24852410-11
AGAGTAC289150.017.87895810-11
CTTATAC86550.017.6387371
GTGGTAT60200.017.6225971
GTACTTT196200.016.77350414-15
Produced by FastQC (version 0.11.5)