##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8593178
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59474003680594	33.0	33.0	33.0	33.0	33.0
2	32.603889736719054	33.0	33.0	33.0	33.0	33.0
3	32.60864374041827	33.0	33.0	33.0	33.0	33.0
4	36.51928995303018	37.0	37.0	37.0	37.0	37.0
5	36.544182373506054	37.0	37.0	37.0	37.0	37.0
6	36.55248174773058	37.0	37.0	37.0	37.0	37.0
7	36.52670013352452	37.0	37.0	37.0	37.0	37.0
8	36.51685895485931	37.0	37.0	37.0	37.0	37.0
9	36.55495545419867	37.0	37.0	37.0	37.0	37.0
10-11	36.50203283348721	37.0	37.0	37.0	37.0	37.0
12-13	36.488242184672536	37.0	37.0	37.0	37.0	37.0
14-15	38.55878424722495	40.0	37.0	40.0	37.0	40.0
16-17	38.49333058153805	40.0	37.0	40.0	37.0	40.0
18-19	38.504415770277305	40.0	37.0	40.0	37.0	40.0
20-21	38.5003197022626	40.0	37.0	40.0	37.0	40.0
22-23	38.4946178301405	40.0	37.0	40.0	37.0	40.0
24-25	38.4890440456616	40.0	37.0	40.0	37.0	40.0
26-27	38.36038899240641	40.0	37.0	40.0	37.0	40.0
28-29	38.30791298381192	40.0	37.0	40.0	37.0	40.0
30-31	38.16189117079688	40.0	37.0	40.0	37.0	40.0
32-33	38.175738691314336	40.0	37.0	40.0	37.0	40.0
34-35	38.14789166825392	40.0	37.0	40.0	37.0	40.0
36-37	38.10059023666905	40.0	37.0	40.0	35.0	40.0
38-39	38.04131917690165	40.0	37.0	40.0	33.0	40.0
40-41	38.01540311441359	40.0	37.0	40.0	33.0	40.0
42-43	37.968988967660806	40.0	37.0	40.0	33.0	40.0
44-45	37.89149512962619	40.0	37.0	40.0	33.0	40.0
46-47	37.742819786841665	40.0	37.0	40.0	33.0	40.0
48-49	37.757612960204625	40.0	37.0	40.0	33.0	40.0
50-51	37.70688340475972	40.0	37.0	40.0	33.0	40.0
52-53	37.639359202166844	40.0	37.0	40.0	33.0	40.0
54-55	37.465286051074585	40.0	37.0	40.0	33.0	40.0
56-57	37.342692548649666	40.0	37.0	40.0	33.0	40.0
58-59	37.20879541186099	37.0	37.0	40.0	33.0	40.0
60-61	37.03791039222996	37.0	37.0	40.0	33.0	40.0
62-63	36.86254349710846	37.0	37.0	40.0	33.0	40.0
64-65	36.684558474606874	37.0	37.0	40.0	33.0	40.0
66-67	36.47590856520638	37.0	37.0	40.0	33.0	40.0
68-69	36.26275290225235	37.0	37.0	40.0	33.0	40.0
70-71	36.0280384055271	37.0	37.0	37.0	33.0	40.0
72-73	35.800744080869435	37.0	35.0	37.0	33.0	40.0
74-75	35.605718558897834	37.0	33.0	37.0	33.0	40.0
76-77	33.92098226980115	35.0	33.0	37.0	27.0	37.0
78-79	35.12250043695437	37.0	33.0	37.0	33.0	37.0
80-81	35.18617110800498	37.0	33.0	37.0	33.0	37.0
82-83	35.04608530475822	37.0	33.0	37.0	33.0	37.0
84-85	34.91500817778163	37.0	33.0	37.0	33.0	37.0
86-87	34.83077660075082	37.0	33.0	37.0	33.0	37.0
88-89	34.6966915529185	37.0	33.0	37.0	33.0	37.0
90-91	34.54792448247233	37.0	33.0	37.0	33.0	37.0
92-93	34.544693704829875	37.0	33.0	37.0	33.0	37.0
94-95	34.60503349357782	37.0	33.0	37.0	33.0	37.0
96-97	34.61376691972832	37.0	33.0	37.0	33.0	37.0
98-99	34.61974083276105	37.0	33.0	37.0	33.0	37.0
100	34.60901671450607	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	7.0
20	69.0
21	396.0
22	1394.0
23	3884.0
24	8796.0
25	17248.0
26	29020.0
27	45158.0
28	66419.0
29	91646.0
30	122913.0
31	162034.0
32	213956.0
33	286693.0
34	396910.0
35	581254.0
36	1034569.0
37	2520302.0
38	2933792.0
39	76715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.49961748726723	20.01274732118897	14.24769741764921	24.239937773894592
2	14.66008268419437	20.680614319870948	41.78108494901421	22.878218046920477
3	18.451392488320387	25.69801300520017	30.169571723057526	25.68102278342192
4	12.790145857562823	17.826303609677353	37.98115202547881	31.40239850728101
5	13.771657005126626	37.421801340551774	35.21273503237103	13.593806621950574
6	30.61806134181139	37.212468986961724	18.438560436440145	13.730909234786736
7	26.992027862101775	32.21706800441001	22.660801393849866	18.13010273963835
8	24.62438227161127	36.931377425208694	20.71948236147325	17.72475794170678
9	25.59338079347854	16.81635716717046	20.77347429791666	36.81678774143434
10-11	24.742924323540493	26.4418850346449	28.361349280998105	20.453841360816497
12-13	25.400394766754154	23.891441528222018	28.558488069980648	22.14967563504318
14-15	22.55074093752951	25.24712707519563	25.962842301337773	26.239289685937077
16-17	22.232863092094682	28.49836230554051	26.67191928294747	22.596855319417333
18-19	22.280918654309268	27.592614746255695	28.28711915428727	21.839347445147766
20-21	23.07997297789529	26.751720738999868	28.116267202298623	22.052039080806217
22-23	22.918335541300785	26.780594916394985	27.960229119636065	22.340840422668165
24-25	22.731185028692344	27.049057815275013	27.946420391825082	22.273336764207563
26-27	22.628356039524753	27.011158002662505	28.041173609136	22.31931234867674
28-29	22.472545515947196	27.000521533557954	28.0140638158107	22.51286913468415
30-31	22.81568975789821	26.8733274733633	27.992500528568986	22.31848224016951
32-33	22.602404953425157	27.03090810945577	28.00673917124309	22.359947765875983
34-35	22.62808462673194	27.000605426226908	28.084708649087364	22.286601297953787
36-37	22.724700153191808	26.932588853568735	27.899045416814545	22.443665576424905
38-39	22.530973217616356	27.000573224407333	27.987793692032064	22.480659865944244
40-41	22.645546167337237	26.875612041668212	27.956899232576987	22.52194255841756
42-43	22.63436287049127	27.020672613045527	27.77083349839359	22.574131018069615
44-45	22.672226681245004	27.08633537716706	27.657636051022255	22.583801890565685
46-47	22.816001879877838	27.020451649353465	27.35761968205019	22.80592678871851
48-49	22.630006302627425	27.254291290489753	27.25184378640815	22.863858620474677
50-51	22.650937235666785	27.305151153468245	27.303667715228652	22.74024389563632
52-53	22.74678879133713	27.296486958782552	27.234275948136688	22.72244830174363
54-55	22.73797842542788	27.203833576804463	27.199806918964597	22.85838107880306
56-57	22.838028044056163	27.301250054044303	27.059931318128623	22.800790583770915
58-59	22.619178912080034	27.429820367031294	27.23605163506767	22.714949085821004
60-61	22.818834694415894	27.492311278183468	27.063621976390085	22.625232051010556
62-63	22.734805679535597	27.647171211047954	26.95433375411451	22.663689355301937
64-65	22.70445544570412	27.466701563559138	26.999467843878094	22.829375146858652
66-67	22.805455639449455	27.470361811174264	26.813964864551785	22.910217684824488
68-69	22.655167359797062	27.4426471544788	26.950604539971824	22.95158094575231
70-71	22.69407991061797	27.276677035501308	27.022594894570712	23.00664815931001
72-73	22.787383590539566	27.304371920302028	26.842641837279857	23.06560265187855
74-75	22.704924781043964	27.442707755757723	26.87798113230174	22.974386330896575
76-77	22.883950899893577	27.32336969265604	26.80351515894624	22.989164248504142
78-79	22.769012444159724	27.294110303967273	26.7921188131579	23.1447584387151
80-81	22.836940651632844	27.37858968154374	26.76532582992841	23.019143836895005
82-83	22.797337071523106	27.36011243674803	26.927783961139696	22.91476653058917
84-85	22.909553215188215	27.32939657582081	26.832737407336122	22.928312801654858
86-87	22.80654974660214	27.348533683986947	26.873377008968845	22.97153956044207
88-89	22.91944820768519	27.30219594868844	26.81961038281629	22.958745460810082
90-91	22.964629102825466	27.32554047186509	26.75891391587273	22.950916509436716
92-93	22.96641502766156	27.252644723232827	26.91152366665952	22.869416582446092
94-95	22.691020352648348	27.42165191459674	26.977220126262182	22.910107606492737
96-97	22.798671336276687	27.419620915975123	27.081482536008828	22.70022521173936
98-99	24.003708395731035	29.346849355207166	27.125590582239344	19.523851666822452
100	22.912266726486173	30.377262243495494	20.8864653962429	25.82400563377543
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1327.0
1	1213.0
2	1687.5
3	2430.5
4	3371.5
5	5656.0
6	7608.5
7	8822.5
8	9445.5
9	9250.0
10	8623.5
11	7792.5
12	8167.0
13	10499.0
14	16819.0
15	26033.5
16	33758.0
17	38513.5
18	40053.0
19	38551.5
20	35066.5
21	30816.0
22	28112.5
23	28584.5
24	32468.5
25	40074.0
26	51076.5
27	63127.0
28	76539.0
29	93144.5
30	110662.0
31	131465.0
32	153724.5
33	175288.0
34	196113.5
35	214389.0
36	236207.0
37	259150.5
38	276200.5
39	286906.5
40	298443.5
41	312260.5
42	321981.5
43	332494.5
44	344980.0
45	352607.0
46	355823.0
47	360480.5
48	371259.0
49	375953.0
50	367883.0
51	358149.0
52	346385.5
53	328347.0
54	308466.0
55	287464.5
56	265623.5
57	242586.5
58	217027.5
59	189647.5
60	159914.0
61	130952.0
62	104458.5
63	81189.0
64	61993.5
65	47113.0
66	35818.0
67	27401.5
68	20741.5
69	15130.0
70	10950.5
71	7969.5
72	5558.5
73	3834.5
74	2619.5
75	1682.5
76	1101.5
77	700.5
78	443.0
79	294.5
80	212.0
81	138.5
82	86.5
83	59.0
84	43.0
85	33.0
86	28.0
87	25.0
88	16.5
89	7.0
90	2.5
91	1.5
92	1.5
93	1.5
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0946848767708522E-4
7	0.0
8	0.0
9	3.491141461284754E-5
10-11	0.006656443052849598
12-13	2.909284551070628E-5
14-15	0.006801907280403129
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	124.0
20-21	468.0
22-23	1044.0
24-25	2975.0
26-27	3228.0
28-29	5113.0
30-31	13095.0
32-33	16737.0
34-35	23386.0
36-37	35380.0
38-39	41079.0
40-41	44828.0
42-43	53272.0
44-45	63840.0
46-47	61734.0
48-49	56887.0
50-51	50364.0
52-53	47757.0
54-55	53949.0
56-57	59946.0
58-59	60677.0
60-61	62616.0
62-63	62625.0
64-65	65814.0
66-67	71570.0
68-69	67374.0
70-71	66377.0
72-73	63527.0
74-75	64937.0
76-77	65187.0
78-79	70472.0
80-81	72751.0
82-83	70412.0
84-85	69869.0
86-87	74672.0
88-89	80343.0
90-91	84442.0
92-93	87986.0
94-95	104889.0
96-97	296420.0
98-99	535464.0
100-101	5759548.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.53787740457764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.34378905919097	75.52934286346333
2	7.414210327179668	12.535632073817366
3	1.8034098155557712	4.573693136929973
4	0.6669291677076656	2.255231048688305
5	0.28562689226986127	1.207314560108002
6	0.16496019043139373	0.8367230613194967
7	0.09583473569226826	0.5671165595937153
8	0.06095047422728418	0.41220989743816155
9	0.037784747265351006	0.2874818098873074
>10	0.12381693481294029	1.609377905072842
>50	0.002312190870958234	0.1350489583238903
>100	3.7546479591788926E-4	0.050828125357687146
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1637138204282513E-5	0.0
4	0.0	0.0	0.0	1.1637138204282513E-5	0.0
5	0.0	0.0	0.0	1.1637138204282513E-5	0.0
6	1.1637138204282514E-4	0.0	0.0	1.1637138204282513E-5	0.0
7	1.2800852024710764E-4	1.1637138204282513E-5	0.0	2.3274276408565027E-5	0.0
8	1.2800852024710764E-4	1.1637138204282513E-5	0.0	2.3274276408565027E-5	2.3274276408565027E-5
9	1.2800852024710764E-4	6.982282922569508E-5	0.0	3.491141461284754E-5	2.3274276408565027E-5
10-11	1.5128279665567267E-4	6.982282922569508E-5	0.0	4.0729983714988796E-5	2.3274276408565027E-5
12-13	1.5128279665567267E-4	6.982282922569508E-5	0.0	4.6548552817130054E-5	2.3274276408565027E-5
14-15	1.6873850396209643E-4	1.2800852024710764E-4	0.0	4.6548552817130054E-5	4.0729983714988796E-5
16-17	3.4329557702633413E-4	1.2800852024710764E-4	0.0	4.6548552817130054E-5	5.818569102141257E-5
18-19	3.491141461284754E-4	1.3964565845139015E-4	0.0	4.6548552817130054E-5	5.818569102141257E-5
20-21	3.607512843327579E-4	1.6291993485995519E-4	0.0	4.6548552817130054E-5	5.818569102141257E-5
22-23	3.723884225370404E-4	1.9201278037066146E-4	0.0	6.400426012355382E-5	7.564139832783634E-5
24-25	4.189369753541705E-4	2.152870567792265E-4	0.0	1.1055281294068388E-4	9.309710563426011E-5
26-27	4.480298208648768E-4	2.3274276408565028E-4	0.0	2.2692419498350903E-4	9.891567473640137E-5
28-29	4.7130409727344177E-4	2.3274276408565028E-4	0.0	5.527640647034193E-4	1.0473424383854262E-4
30-31	5.00396942784148E-4	2.443799022899328E-4	0.0	0.0022343305352222425	1.1637138204282512E-4
32-33	5.120340809884306E-4	2.560170404942153E-4	0.0	0.0069066415242416725	1.3964565845139015E-4
34-35	5.353083573969956E-4	2.851098860049216E-4	0.0	0.012440100740378006	1.3964565845139015E-4
36-37	5.644012029077019E-4	3.2002130061776915E-4	0.0	0.020911937353095676	1.3964565845139015E-4
38-39	5.702197720098432E-4	3.4329557702633413E-4	0.0	0.04291776569739391	1.3964565845139015E-4
40-41	5.760383411119844E-4	4.53848389967018E-4	0.0	0.06753613156855356	1.3964565845139015E-4
42-43	5.818569102141257E-4	4.7712266637558304E-4	0.0	0.08318808245331355	1.454642275535314E-4
44-45	5.993126175205495E-4	5.294897882948544E-4	0.0	0.1020809763279662	1.5128279665567267E-4
46-47	6.167683248269732E-4	5.585826338055606E-4	0.0	0.12088659166608676	1.5710136575781394E-4
48-49	6.342240321333969E-4	5.644012029077019E-4	0.0	0.13827247614328483	1.745570730642377E-4
50-51	7.098654304612333E-4	6.57498308541962E-4	0.0	0.1550823222793709	1.745570730642377E-4
52-53	7.564139832783633E-4	6.633168776441033E-4	0.0	0.1985586706105704	1.8037564216637897E-4
54-55	7.680511214826458E-4	6.633168776441033E-4	0.0	0.2486274577344959	1.9201278037066146E-4
56-57	7.680511214826458E-4	6.865911540526683E-4	0.0	0.29932465032145267	1.9783134947280273E-4
58-59	7.680511214826458E-4	6.924097231548095E-4	0.0	0.33697661098140874	1.9783134947280273E-4
60-61	8.204182434019172E-4	7.040468613590921E-4	0.0	0.3640562315827741	1.9783134947280273E-4
62-63	9.07696779934036E-4	7.098654304612333E-4	0.0	0.3836706280260923	2.0946848767708525E-4
64-65	9.135153490361773E-4	8.37873950708341E-4	0.0	0.38938446288439504	2.0946848767708525E-4
66-67	9.251524872404598E-4	8.61148227116906E-4	0.0	0.3944349808650537	2.0946848767708525E-4
68-69	9.309710563426011E-4	8.786039344233298E-4	0.0	0.4000964486014371	2.0946848767708525E-4
70-71	9.484267636490248E-4	8.960596417297536E-4	0.0	0.40467566248482223	2.3274276408565028E-4
72-73	0.0010124310237725786	9.018782108318947E-4	0.0	0.4080387954258599	2.560170404942153E-4
74-75	0.0010124310237725786	9.30971056342601E-4	0.0	0.4109422614078284	2.676541786984978E-4
76-77	0.0010240681619768611	0.001000793885568296	0.0	0.413933005926329	2.676541786984978E-4
78-79	0.0010357053001811437	0.0010357053001811437	0.0	0.41581822231542276	2.676541786984978E-4
80-81	0.0010357053001811437	0.0010357053001811437	0.0	0.4164524463475562	2.676541786984978E-4
82-83	0.001041523869283285	0.0010473424383854262	0.0	0.4168772018920125	2.9092845510706285E-4
84-85	0.0010473424383854262	0.0010473424383854262	0.0	0.417104126086996	2.9092845510706285E-4
86-87	0.0010473424383854262	0.0010647981456918501	0.0	0.4171914046235281	2.9092845510706285E-4
88	0.0010589795765897087	0.0011171652676111212	0.0	0.4172030417617324	3.0256559331134534E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11340	0.0	45.445007	1
GTATCAA	19610	0.0	33.536945	1
TCAACGC	21735	0.0	29.208536	4
ATCAACG	21905	0.0	28.99952	3
CAACGCA	22225	0.0	28.469366	5
TATCAAC	23085	0.0	27.741367	2
AACGCAG	22925	0.0	27.712954	6
ACGCAGA	26645	0.0	23.860043	7
CGCAGAG	27395	0.0	23.238577	8
GTACATG	26120	0.0	22.8174	1
TACATGG	25945	0.0	22.489275	2
ACATGGG	26325	0.0	21.705952	3
GCAGAGT	32220	0.0	19.774134	9
GAGTACT	17130	0.0	19.311783	12-13
CATGGGG	21100	0.0	19.070446	4
CAGAGTA	31225	0.0	18.248524	10-11
AGAGTAC	28915	0.0	17.878958	10-11
CTTATAC	8655	0.0	17.638737	1
GTGGTAT	6020	0.0	17.622597	1
GTACTTT	19620	0.0	16.773504	14-15
>>END_MODULE