FastQCFastQC Report
Fri 27 May 2016
SRR2139115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9558812
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126550.047.6424751
GTATCAA220600.035.373751
TCAACGC256250.029.8168224
ATCAACG260300.029.300353
CAACGCA264550.028.8331095
AACGCAG271600.028.2274026
TATCAAC273400.028.1484492
ACGCAGA312550.024.4601757
CGCAGAG316800.024.1460068
GTACATG306850.022.680031
TACATGG307450.021.9494152
ACATGGG313100.021.0856763
GCAGAGT377300.020.1623619
CATGGGG240050.019.4490854
GTGGTAT65150.019.4339621
GAGTACT201600.019.20677412-13
TGGTATC61950.018.6999052
CAGAGTA362750.018.61298610-11
AGAGTAC337200.018.0497210-11
GTACTTT219350.017.69204714-15
Produced by FastQC (version 0.11.5)