FastQCFastQC Report
Fri 27 May 2016
SRR2139114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10014471
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123700.047.8891751
GTATCAA221250.037.569941
TCAACGC264300.030.7067584
ATCAACG268000.030.2949183
CAACGCA272950.029.7026025
TATCAAC282300.028.9429262
AACGCAG280500.028.903126
CTTATAC62900.025.9522911
ACGCAGA322250.025.185167
CGCAGAG328500.024.6668768
GTACATG306400.022.432311
TACATGG308900.021.7223052
TATACAC98600.020.9561253
ACATGGG311400.020.8995463
GCAGAGT391850.020.8135959
GAGTACT210550.019.91601412-13
GTGGTAT64100.019.7700041
TGGTATC61100.018.9127142
CAGAGTA374150.018.64442810-11
AGAGTAC343700.018.5960210-11
Produced by FastQC (version 0.11.5)