##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139114_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10014471
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.617527575844996	33.0	33.0	33.0	33.0	33.0
2	32.62292706224822	33.0	33.0	33.0	33.0	33.0
3	32.628301484921174	33.0	33.0	33.0	33.0	33.0
4	36.5446894798537	37.0	37.0	37.0	37.0	37.0
5	36.56988791519792	37.0	37.0	37.0	37.0	37.0
6	36.576240322629126	37.0	37.0	37.0	37.0	37.0
7	36.55153447446201	37.0	37.0	37.0	37.0	37.0
8	36.54327542613085	37.0	37.0	37.0	37.0	37.0
9	36.5767232238228	37.0	37.0	37.0	37.0	37.0
10-11	36.528745302672505	37.0	37.0	37.0	37.0	37.0
12-13	36.51429111932123	37.0	37.0	37.0	37.0	37.0
14-15	38.612268785840016	40.0	37.0	40.0	37.0	40.0
16-17	38.55382665744401	40.0	37.0	40.0	37.0	40.0
18-19	38.56764161581775	40.0	37.0	40.0	37.0	40.0
20-21	38.561342201060256	40.0	37.0	40.0	37.0	40.0
22-23	38.55782434332586	40.0	37.0	40.0	37.0	40.0
24-25	38.555275175399345	40.0	37.0	40.0	37.0	40.0
26-27	38.435624296965	40.0	37.0	40.0	37.0	40.0
28-29	38.38696049371188	40.0	37.0	40.0	37.0	40.0
30-31	38.23643517230312	40.0	37.0	40.0	37.0	40.0
32-33	38.25395164779496	40.0	37.0	40.0	37.0	40.0
34-35	38.22941337316894	40.0	37.0	40.0	37.0	40.0
36-37	38.18526767797994	40.0	37.0	40.0	37.0	40.0
38-39	38.13328881052423	40.0	37.0	40.0	37.0	40.0
40-41	38.10848108195076	40.0	37.0	40.0	37.0	40.0
42-43	38.062175418399136	40.0	37.0	40.0	33.0	40.0
44-45	37.988864754329505	40.0	37.0	40.0	33.0	40.0
46-47	37.83869636423175	40.0	37.0	40.0	33.0	40.0
48-49	37.85593334564483	40.0	37.0	40.0	33.0	40.0
50-51	37.818691170038505	40.0	37.0	40.0	33.0	40.0
52-53	37.755199749930284	40.0	37.0	40.0	33.0	40.0
54-55	37.585759592455894	40.0	37.0	40.0	33.0	40.0
56-57	37.46887324505768	40.0	37.0	40.0	33.0	40.0
58-59	37.337850470194155	38.5	37.0	40.0	33.0	40.0
60-61	37.16781028408877	37.0	37.0	40.0	33.0	40.0
62-63	36.99657470575812	37.0	37.0	40.0	33.0	40.0
64-65	36.82047577689772	37.0	37.0	40.0	33.0	40.0
66-67	36.61285944066881	37.0	37.0	40.0	33.0	40.0
68-69	36.40019062165902	37.0	37.0	40.0	33.0	40.0
70-71	36.16262638235467	37.0	37.0	38.5	33.0	40.0
72-73	35.93650343794389	37.0	37.0	37.0	33.0	40.0
74-75	35.74178923702212	37.0	35.0	37.0	33.0	40.0
76-77	34.05852471593168	35.0	33.0	37.0	30.0	37.0
78-79	35.24315707902633	37.0	33.0	37.0	33.0	37.0
80-81	35.30299498874764	37.0	33.0	37.0	33.0	37.0
82-83	35.16493139411447	37.0	33.0	37.0	33.0	37.0
84-85	35.03707131582499	37.0	33.0	37.0	33.0	37.0
86-87	34.951168974746935	37.0	33.0	37.0	33.0	37.0
88-89	34.81745023584163	37.0	33.0	37.0	33.0	37.0
90-91	34.673646161051025	37.0	33.0	37.0	33.0	37.0
92-93	34.671406032931586	37.0	33.0	37.0	33.0	37.0
94-95	34.73300209617213	37.0	33.0	37.0	33.0	37.0
96-97	34.739694989214144	37.0	33.0	37.0	33.0	37.0
98-99	34.7386411178554	37.0	33.0	37.0	33.0	37.0
100	34.72714578685277	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	15.0
20	67.0
21	371.0
22	1407.0
23	3926.0
24	8759.0
25	17498.0
26	30364.0
27	47575.0
28	70469.0
29	98048.0
30	133448.0
31	177617.0
32	236191.0
33	318039.0
34	441700.0
35	648399.0
36	1154591.0
37	2900798.0
38	3624377.0
39	100810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.668491525912856	19.93054850326093	14.296561445931594	24.104398524894624
2	14.701425567061904	20.641230075957083	41.792532026903864	22.864812330077143
3	18.36825929197858	25.819306881012487	30.101789700124947	25.710644126883984
4	12.686111927429817	17.895523388105072	38.155684908369096	31.262679776096014
5	13.726835895775224	37.50121199612042	35.15331963116174	13.618632476942615
6	30.528996441443184	37.19218613338142	18.624152512373865	13.654664912801529
7	26.965648010763626	32.28305319372337	22.709936450961813	18.0413623445512
8	24.62304798725764	36.752235839516636	20.824684598916907	17.800031574308818
9	25.74472995023501	16.815240029201746	20.817648943743297	36.62238107681995
10-11	24.640318599456602	26.504121426098067	28.470372592298	20.385187382147336
12-13	25.430051615829957	23.94854983038787	28.583554600736843	22.03784395304533
14-15	22.484929228339343	25.335333781916354	26.12782379115764	26.05191319858666
16-17	22.208836592566897	28.4422462254871	26.813672933897358	22.53524424804865
18-19	22.232487367530446	27.574576829869496	28.463879919368683	21.72905588323138
20-21	23.03124889532328	26.73364094856106	28.332104416889464	21.903005739226195
22-23	22.764871608034944	26.755255424020817	28.162965018698937	22.316907949245298
24-25	22.52054403198649	26.92608243682222	28.264392933187604	22.28898059800369
26-27	22.475957737712626	27.04876712514905	28.241624617342282	22.23365051979604
28-29	22.40664902022677	27.0150905647787	28.271684740573942	22.306575674420586
30-31	22.72908527554899	26.952076296332162	28.073407968729803	22.24543045938904
32-33	22.443755733861344	27.00011890504538	28.157636722888764	22.398488638204515
34-35	22.499044579110105	27.15185140731865	28.203020285054404	22.146083728516842
36-37	22.619121812695756	27.050721731217813	27.99090723913771	22.339249216948723
38-39	22.394624483456326	27.08807761827329	28.106662560436803	22.410635337833583
40-41	22.635490136459964	26.94167402032613	28.045720506108214	22.377115337105696
42-43	22.565610931079902	27.04601417459059	27.85996366245732	22.528411231872187
44-45	22.581988301729204	27.200217500250883	27.65378308413664	22.564011113883268
46-47	22.797045380091415	27.14406502035786	27.33581891643137	22.723070683119353
48-49	22.638035308195516	27.274161456169427	27.32384805063714	22.76395518499792
50-51	22.618011991678813	27.39980728469756	27.291978358830654	22.690202364792974
52-53	22.78362063564344	27.30937931955843	27.271670243697027	22.635329801101097
54-55	22.69556095390999	27.253400091152074	27.19287460019307	22.85816435474487
56-57	22.77437552004729	27.425211579793274	27.085448250705678	22.71496464945376
58-59	22.676795790865704	27.505466062376687	27.20476470663672	22.612973440120886
60-61	22.797515092417086	27.530677871393983	27.080836191123137	22.590970845065794
62-63	22.70296146450595	27.658078046079478	27.036291613070972	22.6026688763436
64-65	22.736958233180673	27.533043278088304	26.99557945471589	22.734419034015136
66-67	22.793908717517482	27.469605928493774	26.942896909717163	22.793588444271585
68-69	22.73654247517363	27.42963200956639	27.03153340178025	22.802292113479737
70-71	22.752367385695287	27.26904391080749	27.086185431496407	22.892403272000816
72-73	22.79176475809616	27.282786748294434	27.01958977539436	22.905858718215043
74-75	22.663358142414474	27.42279705848763	26.99607822410271	22.917766574995188
76-77	22.837280686222226	27.336036139684026	26.929810605640164	22.89687256845358
78-79	22.767837747892	27.353869706730343	26.907989353968688	22.97030319140897
80-81	22.788785878645086	27.402005064614897	26.925374860270594	22.883834196469422
82-83	22.799870403240234	27.364951935961933	26.965055608533145	22.870122052264687
84-85	22.872268878727827	27.358107348611803	26.931479504260814	22.838144268399553
86-87	22.777556736057456	27.355741597918442	27.023774008425377	22.84292765759872
88-89	22.818162983378052	27.307677368120764	26.959419409210305	22.914740239290882
90-91	22.862648047292364	27.35254951480713	26.94035511666886	22.844447321231648
92-93	22.800819384539416	27.317071239273133	27.03339932057705	22.8487100556104
94-95	22.72614147032319	27.37671584706738	27.022859496839736	22.8742831857697
96-97	22.842604955654284	27.465682870721388	27.05149197858283	22.640220195041497
98-99	23.983671032723123	29.392720068801697	27.21821438088191	19.405394517593265
100	22.858607605152194	30.362170709493093	21.03118687645674	25.748034808897973
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1787.0
1	1629.0
2	2228.0
3	3158.0
4	4220.0
5	7014.0
6	9499.0
7	11021.5
8	11793.5
9	11496.0
10	10616.0
11	9746.5
12	10581.5
13	13979.0
14	22126.5
15	33689.5
16	43651.0
17	50073.5
18	51898.5
19	49171.5
20	43994.0
21	38434.0
22	34680.0
23	34610.0
24	38875.0
25	47316.5
26	59330.5
27	74094.5
28	91664.0
29	112510.5
30	134837.5
31	157904.5
32	182296.0
33	206604.0
34	231187.5
35	253904.0
36	277642.5
37	301798.0
38	322944.0
39	340367.0
40	356008.0
41	371188.0
42	381743.5
43	391375.0
44	400894.0
45	411885.0
46	422649.0
47	430778.5
48	432418.5
49	425961.0
50	419028.5
51	409084.0
52	394312.0
53	375493.0
54	354720.5
55	333282.0
56	309330.0
57	281702.0
58	250557.0
59	217589.0
60	184048.0
61	151810.5
62	122411.0
63	96347.5
64	72998.0
65	54420.5
66	40930.5
67	31349.5
68	24034.0
69	17603.0
70	12676.5
71	9231.0
72	6552.5
73	4481.0
74	3064.0
75	2062.5
76	1343.0
77	830.0
78	509.0
79	329.0
80	225.5
81	150.5
82	108.0
83	83.0
84	65.5
85	50.5
86	38.0
87	24.0
88	11.0
89	3.5
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.7973989839303544E-4
7	0.0
8	0.0
9	1.9971099821448385E-5
10-11	0.006425701367551016
12-13	4.493497459825886E-5
14-15	0.007159639285989245
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	140.0
20-21	533.0
22-23	1235.0
24-25	2926.0
26-27	3684.0
28-29	6255.0
30-31	16389.0
32-33	20579.0
34-35	28781.0
36-37	43113.0
38-39	53384.0
40-41	55739.0
42-43	66610.0
44-45	80054.0
46-47	77184.0
48-49	70297.0
50-51	63362.0
52-53	62838.0
54-55	68437.0
56-57	73428.0
58-59	73535.0
60-61	74582.0
62-63	73443.0
64-65	77501.0
66-67	83386.0
68-69	78056.0
70-71	77188.0
72-73	73499.0
74-75	76118.0
76-77	76000.0
78-79	81572.0
80-81	83779.0
82-83	81571.0
84-85	81454.0
86-87	85454.0
88-89	92555.0
90-91	96238.0
92-93	99159.0
94-95	119977.0
96-97	336542.0
98-99	619027.0
100-101	6678867.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.5094854640569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.67337139780722	75.78250476655964
2	7.191823472722353	12.155546024561858
3	1.7213285178377928	4.364057620712386
4	0.6277741375012469	2.1221147739148987
5	0.29079137463504295	1.228731472389664
6	0.1614875780674424	0.8188339278789765
7	0.08608459199355174	0.5092475203030831
8	0.056016823888706734	0.3787162371332237
9	0.04001707603265006	0.3043640254765769
>10	0.14613291011322174	1.9709018425405396
>50	0.004034475698395864	0.22408474293403266
>100	0.001137643702425747	0.14089704559510755
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9956649732172574E-5	0.0	0.0	0.0
3	0.0	3.994219964289676E-5	0.0	0.0	0.0
4	0.0	5.991329946434515E-5	0.0	0.0	9.98554991072419E-6
5	0.0	7.988439928579353E-5	0.0	0.0	9.98554991072419E-6
6	3.994219964289676E-5	7.988439928579353E-5	0.0	9.98554991072419E-6	1.997109982144838E-5
7	3.994219964289676E-5	8.986994919651772E-5	0.0	9.98554991072419E-6	1.997109982144838E-5
8	3.994219964289676E-5	9.985549910724192E-5	0.0	9.98554991072419E-6	1.997109982144838E-5
9	4.992774955362096E-5	1.098410490179661E-4	0.0	9.98554991072419E-6	1.997109982144838E-5
10-11	4.992774955362096E-5	1.098410490179661E-4	0.0	9.98554991072419E-6	2.4963874776810476E-5
12-13	6.989884937506934E-5	1.198265989286903E-4	0.0	9.98554991072419E-6	3.994219964289676E-5
14-15	6.989884937506934E-5	1.3979769875013869E-4	0.0	9.98554991072419E-6	6.989884937506934E-5
16-17	1.098410490179661E-4	1.4978324866086285E-4	0.0	9.98554991072419E-6	1.098410490179661E-4
18-19	1.198265989286903E-4	1.4978324866086285E-4	0.0	1.997109982144838E-5	1.2481937388405238E-4
20-21	1.298121488394145E-4	1.7973989839303544E-4	0.0	2.9956649732172574E-5	1.348049237947766E-4
22-23	1.298121488394145E-4	1.9971099821448383E-4	0.0	4.493497459825886E-5	1.6975434848231125E-4
24-25	1.298121488394145E-4	2.1468932308057011E-4	0.0	5.991329946434515E-5	2.0470377316984592E-4
26-27	1.3979769875013869E-4	2.296676479466564E-4	0.0	6.490607441970725E-5	2.09696548125208E-4
28-29	1.3979769875013869E-4	2.296676479466564E-4	0.0	1.5976879857158708E-4	2.09696548125208E-4
30-31	1.3979769875013869E-4	2.4963874776810476E-4	0.0	5.442124701344683E-4	2.09696548125208E-4
32-33	1.3979769875013869E-4	2.4963874776810476E-4	0.0	0.0014728686118318183	2.09696548125208E-4
34-35	1.3979769875013869E-4	2.7460262254491523E-4	0.0	0.0030006577481726197	2.1468932308057011E-4
36-37	1.3979769875013869E-4	3.0455927227708785E-4	0.0	0.00490789778112094	2.296676479466564E-4
38-39	1.3979769875013869E-4	3.445014719199846E-4	0.0	0.008168179826972388	2.446459728127427E-4
40-41	1.5477602361622494E-4	3.794508966075193E-4	0.0	0.01179293444456527	2.4963874776810476E-4
42-43	1.5976879857158705E-4	3.8444367156288136E-4	0.0	0.015312840788095548	2.7460262254491523E-4
44-45	1.6476157352694916E-4	4.443569710272265E-4	0.0	0.01931704630229595	2.945737223663636E-4
46-47	1.6975434848231125E-4	4.992774955362095E-4	0.0	0.023735652137791403	3.14544822187812E-4
48-49	1.6975434848231125E-4	5.042702704915717E-4	0.0	0.028598614944314083	3.445014719199846E-4
50-51	1.8972544830375964E-4	5.492052450898305E-4	0.0	0.0331020979540507	3.4949424687534666E-4
52-53	2.196820980359322E-4	5.492052450898305E-4	0.0	0.037675479813162376	3.844436715628813E-4
54-55	2.196820980359322E-4	5.741691198666409E-4	0.0	0.04239864492093491	4.343714211165023E-4
56-57	2.196820980359322E-4	5.791618948220031E-4	0.0	0.04718671610312716	4.543425209379507E-4
58-59	2.196820980359322E-4	5.891474447327273E-4	0.0	0.0523192887572394	4.593352958933128E-4
60-61	2.2467487299129428E-4	6.041257695988136E-4	0.0	0.05746184696126236	4.593352958933128E-4
62-63	2.296676479466564E-4	6.340824193309862E-4	0.0	0.062489571341311986	4.892919456254853E-4
64-65	2.296676479466564E-4	7.089740436614176E-4	0.0	0.06723270754890598	4.992774955362095E-4
66-67	2.396531978573806E-4	7.339379184382281E-4	0.0	0.07259994062592023	5.092630454469338E-4
68-69	2.396531978573806E-4	7.489162433043143E-4	0.0	0.07801210867753275	5.19248595357658E-4
70-71	2.396531978573806E-4	7.589017932150386E-4	0.0	0.08271530268548384	5.292341452683821E-4
72-73	2.4963874776810476E-4	7.589017932150386E-4	0.0	0.08606046190557644	5.891474447327273E-4
74-75	2.4963874776810476E-4	7.638945681704007E-4	0.0	0.08887139420544529	5.891474447327273E-4
76-77	2.4963874776810476E-4	7.938512179025732E-4	0.0	0.09164238430567126	5.941402196880893E-4
78-79	2.4963874776810476E-4	8.088295427686595E-4	0.0	0.09340483386491408	5.991329946434514E-4
80-81	2.4963874776810476E-4	8.088295427686595E-4	0.0	0.09395403911000391	6.240968694202619E-4
82-83	2.4963874776810476E-4	8.088295427686595E-4	0.0	0.0945082371300491	6.890029438399692E-4
84-85	2.4963874776810476E-4	8.088295427686595E-4	0.0	0.09473790477799576	6.890029438399692E-4
86-87	2.4963874776810476E-4	8.188150926793837E-4	0.0	0.0947978180774601	6.890029438399692E-4
88	2.4963874776810476E-4	8.687428422330046E-4	0.0	0.09480281085241547	6.890029438399692E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12370	0.0	47.889175	1
GTATCAA	22125	0.0	37.56994	1
TCAACGC	26430	0.0	30.706758	4
ATCAACG	26800	0.0	30.294918	3
CAACGCA	27295	0.0	29.702602	5
TATCAAC	28230	0.0	28.942926	2
AACGCAG	28050	0.0	28.90312	6
CTTATAC	6290	0.0	25.952291	1
ACGCAGA	32225	0.0	25.18516	7
CGCAGAG	32850	0.0	24.666876	8
GTACATG	30640	0.0	22.43231	1
TACATGG	30890	0.0	21.722305	2
TATACAC	9860	0.0	20.956125	3
ACATGGG	31140	0.0	20.899546	3
GCAGAGT	39185	0.0	20.813595	9
GAGTACT	21055	0.0	19.916014	12-13
GTGGTAT	6410	0.0	19.770004	1
TGGTATC	6110	0.0	18.912714	2
CAGAGTA	37415	0.0	18.644428	10-11
AGAGTAC	34370	0.0	18.59602	10-11
>>END_MODULE