FastQCFastQC Report
Fri 27 May 2016
SRR2139113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8948409
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128400.043.6908951
CTTATAC69550.034.806661
GTATCAA217200.033.555081
TCAACGC248800.028.4504724
ATCAACG251200.028.1248723
CAACGCA253450.027.8781555
AACGCAG259550.027.2064176
TATCAAC263650.027.0898082
TATACAC111850.025.6726383
TTATACA104650.024.157752
ACGCAGA300150.023.4977177
CGCAGAG301600.023.385148
GTACATG275150.020.4821531
TACATGG269200.020.1854762
GAGTACT191050.019.92958312-13
GCAGAGT355600.019.8113739
ACATGGG272400.019.3338283
TACACAT140400.019.2619365
ACATCTC130150.018.8992358
ACACATC132350.018.6172036
Produced by FastQC (version 0.11.5)