##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139113_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8948409
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.621204506857026	33.0	33.0	33.0	33.0	33.0
2	32.62909205424115	33.0	33.0	33.0	33.0	33.0
3	32.63429119075804	33.0	33.0	33.0	33.0	33.0
4	36.552586945902895	37.0	37.0	37.0	37.0	37.0
5	36.57625282885483	37.0	37.0	37.0	37.0	37.0
6	36.58039032413471	37.0	37.0	37.0	37.0	37.0
7	36.555180703072466	37.0	37.0	37.0	37.0	37.0
8	36.54705534805126	37.0	37.0	37.0	37.0	37.0
9	36.58184544313967	37.0	37.0	37.0	37.0	37.0
10-11	36.53388585613375	37.0	37.0	37.0	37.0	37.0
12-13	36.5209158410171	37.0	37.0	37.0	37.0	37.0
14-15	38.62199554133031	40.0	37.0	40.0	37.0	40.0
16-17	38.561997445579436	40.0	37.0	40.0	37.0	40.0
18-19	38.57707699770987	40.0	37.0	40.0	37.0	40.0
20-21	38.573109476455784	40.0	37.0	40.0	37.0	40.0
22-23	38.56861805618426	40.0	37.0	40.0	37.0	40.0
24-25	38.565014513958815	40.0	37.0	40.0	37.0	40.0
26-27	38.442884178204835	40.0	37.0	40.0	37.0	40.0
28-29	38.3950419286818	40.0	37.0	40.0	37.0	40.0
30-31	38.24064915566396	40.0	37.0	40.0	37.0	40.0
32-33	38.26378249272727	40.0	37.0	40.0	37.0	40.0
34-35	38.24170379350963	40.0	37.0	40.0	37.0	40.0
36-37	38.19373655427217	40.0	37.0	40.0	37.0	40.0
38-39	38.13951887750372	40.0	37.0	40.0	37.0	40.0
40-41	38.11649267538028	40.0	37.0	40.0	37.0	40.0
42-43	38.07209352573959	40.0	37.0	40.0	33.0	40.0
44-45	38.000473802073685	40.0	37.0	40.0	33.0	40.0
46-47	37.85440561725129	40.0	37.0	40.0	33.0	40.0
48-49	37.87440402228759	40.0	37.0	40.0	33.0	40.0
50-51	37.8299392335491	40.0	37.0	40.0	33.0	40.0
52-53	37.77127684066515	40.0	37.0	40.0	33.0	40.0
54-55	37.60844515409052	40.0	37.0	40.0	33.0	40.0
56-57	37.49291650392838	40.0	37.0	40.0	33.0	40.0
58-59	37.370375672796406	40.0	37.0	40.0	33.0	40.0
60-61	37.204810520542665	37.0	37.0	40.0	33.0	40.0
62-63	37.03955961568981	37.0	37.0	40.0	33.0	40.0
64-65	36.87079726630263	37.0	37.0	40.0	33.0	40.0
66-67	36.66974960847509	37.0	37.0	40.0	33.0	40.0
68-69	36.45970220274363	37.0	37.0	40.0	33.0	40.0
70-71	36.225807880948196	37.0	37.0	38.5	33.0	40.0
72-73	35.99948359806798	37.0	37.0	37.0	33.0	40.0
74-75	35.80344794546482	37.0	37.0	37.0	33.0	40.0
76-77	34.12956022935558	35.0	33.0	37.0	30.0	37.0
78-79	35.303590473451855	37.0	33.0	37.0	33.0	37.0
80-81	35.35652557898666	37.0	33.0	37.0	33.0	37.0
82-83	35.212321385525385	37.0	33.0	37.0	33.0	37.0
84-85	35.07934574645356	37.0	33.0	37.0	33.0	37.0
86-87	34.99316387958252	37.0	33.0	37.0	33.0	37.0
88-89	34.85431692538505	37.0	33.0	37.0	33.0	37.0
90-91	34.70769847365161	37.0	33.0	37.0	33.0	37.0
92-93	34.70583474689573	37.0	33.0	37.0	33.0	37.0
94-95	34.76260099984067	37.0	33.0	37.0	33.0	37.0
96-97	34.76547438720247	37.0	33.0	37.0	33.0	37.0
98-99	34.76663401090707	37.0	33.0	37.0	33.0	37.0
100	34.756643459169524	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	10.0
20	76.0
21	356.0
22	1214.0
23	3504.0
24	7983.0
25	15511.0
26	26883.0
27	42134.0
28	62319.0
29	86905.0
30	117475.0
31	156896.0
32	209052.0
33	280842.0
34	389299.0
35	572823.0
36	1017300.0
37	2560448.0
38	3303614.0
39	93760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.22437854595158	20.200820056392146	14.437069204145676	24.1377321935106
2	14.486496985106514	20.554815945493772	42.19068439987488	22.76800266952483
3	18.266990254915708	25.875650073661138	30.28292515462805	25.574434516795108
4	12.488231148129238	18.035731267982946	38.42250616841497	31.05353141547285
5	13.510457557315497	37.554374190987474	35.45430254696673	13.480865704730306
6	30.16307624465672	37.595965576381275	18.62229757282622	13.618660606135785
7	26.66353314874186	32.41074474803286	22.808155058625506	18.117567044599774
8	24.28384755323544	37.151296951223394	20.86128383269026	17.703571662850905
9	25.37210537832101	16.95077313210867	21.0246271794105	36.652494310159824
10-11	24.409224948819947	26.670289149694753	28.587906643650264	20.332579257835036
12-13	25.194847573394362	24.052923398080438	28.729466312711594	22.022762715813602
14-15	22.32060369995046	25.525483993452035	26.210522168941104	25.943390137656397
16-17	21.944068493069548	28.740081057984725	26.94187871832859	22.37397173061714
18-19	22.00045840551097	27.782150994662853	28.63630842086007	21.581082178966117
20-21	22.750231847877174	26.912223234652405	28.512972389525892	21.824572527944536
22-23	22.48248922737458	26.976293602163317	28.373511962223013	22.16770520823909
24-25	22.363890111780524	27.13829999911136	28.39830469494072	22.099505194167403
26-27	22.327544640873075	27.26570581956039	28.379014240524462	22.02773529904207
28-29	22.128143462282424	27.193816680509197	28.443922869348555	22.234116987859824
30-31	22.333591468822654	27.09421814753129	28.48132650482402	22.090863878822038
32-33	21.975945619303165	27.118360774219013	28.64464574021706	22.26104786626076
34-35	22.137360823938135	27.22352807758877	28.455702692895617	22.183408405577477
36-37	22.24410512136523	27.23047412196347	28.377620690024592	22.147800066646706
38-39	22.194150897249074	27.24028709840129	28.368697329557918	22.19686467479172
40-41	22.30559469119281	27.208952818458048	28.335386945475587	22.150065544873556
42-43	22.300837000588196	27.273671272454536	28.18725097318453	22.238240753772736
44-45	22.360881608695436	27.418306360205058	27.999201067014255	22.22161096408525
46-47	22.50712219201686	27.308236362472133	27.562399948773773	22.62224149673724
48-49	22.371344501258676	27.48010668918866	27.55369596257205	22.59485284698061
50-51	22.326176891244774	27.55435136657111	27.628972435427624	22.490499306756494
52-53	22.41929791037258	27.604580604314176	27.593023700757367	22.38309778455587
54-55	22.37520075798593	27.539156683724066	27.511207420745055	22.574435137544953
56-57	22.572693759925002	27.648496775374333	27.294956754317774	22.48385271038289
58-59	22.32505908135302	27.848082994181212	27.52658737017846	22.3002705542873
60-61	22.57395163666288	27.899258241427464	27.257551196625364	22.26923892528429
62-63	22.449999451695728	28.0368544731685	27.1855950492275	22.327551025908278
64-65	22.424700840242558	27.868433259679815	27.200346098020407	22.50651980205723
66-67	22.50890293645204	27.793385013193717	27.144642929455593	22.553069120898652
68-69	22.439499476743283	27.68171932810221	27.29025097220818	22.58853022294633
70-71	22.441161486733748	27.53146459244526	27.33188516738175	22.695488753439243
72-73	22.54161944228535	27.608755601159356	27.174962995868963	22.674661960686336
74-75	22.445853589816203	27.676061022386474	27.236010562883777	22.64207482491355
76-77	22.555206398822236	27.58559437144933	27.17777834022507	22.68142088950336
78-79	22.442917342425712	27.572425652316703	27.194856299253704	22.78980070600388
80-81	22.50384559369729	27.632879539064575	27.192881443312466	22.670393423925667
82-83	22.48474844382427	27.64753284996198	27.256387855167596	22.61133085104616
84-85	22.597393120572075	27.618886198812408	27.17302701509759	22.61069366551792
86-87	22.47549431293871	27.624401657559766	27.249632786228016	22.650471243273508
88-89	22.58649422589428	27.540121707776155	27.175104562057665	22.6982795042719
90-91	22.572551282913686	27.633200758143172	27.176086269593043	22.6181616893501
92-93	22.606480165803546	27.54626110340138	27.27413864836813	22.573120082426936
94-95	22.425204778867798	27.638038976350142	27.295115188679077	22.641641056102983
96-97	22.471724664677577	27.694780950031202	27.405699018496716	22.427795366794513
98-99	23.67244437170055	29.60916656170661	27.482015066936818	19.236373999656028
100	22.59426333700255	30.564138165808274	21.265077774041803	25.57652072314737
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1587.0
1	1459.5
2	2033.0
3	2866.5
4	3926.0
5	6689.0
6	8960.5
7	10146.5
8	10653.5
9	10135.5
10	9102.5
11	8239.5
12	9107.5
13	12459.5
14	20276.5
15	31116.5
16	40330.0
17	46299.5
18	48215.0
19	46151.0
20	41798.5
21	36726.5
22	33549.5
23	34434.5
24	39568.5
25	48681.0
26	61017.5
27	75343.0
28	91003.5
29	110602.5
30	131124.0
31	151689.5
32	174073.5
33	195905.0
34	217501.5
35	237985.5
36	259678.5
37	281442.0
38	297409.5
39	306567.0
40	317590.5
41	330709.5
42	340855.0
43	351878.0
44	363441.0
45	370700.5
46	373773.0
47	377267.0
48	387052.0
49	390035.5
50	379231.5
51	366599.0
52	352567.5
53	332522.5
54	308672.5
55	284565.5
56	261292.5
57	237330.0
58	210808.0
59	182347.0
60	151228.0
61	121718.5
62	96130.0
63	74256.0
64	55593.5
65	41395.5
66	31568.0
67	24419.5
68	18747.5
69	13923.0
70	10243.5
71	7427.0
72	5255.5
73	3658.0
74	2540.0
75	1731.5
76	1119.0
77	668.5
78	417.5
79	264.0
80	192.5
81	148.0
82	105.0
83	74.0
84	55.0
85	44.0
86	35.0
87	26.5
88	22.5
89	18.5
90	14.0
91	8.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0115307648544003E-4
7	0.0
8	0.0
9	3.352551274757334E-5
10-11	0.006559825327608517
12-13	4.470068366343112E-5
14-15	0.006554237742150588
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	113.0
20-21	490.0
22-23	1130.0
24-25	1988.0
26-27	3044.0
28-29	5752.0
30-31	14154.0
32-33	18655.0
34-35	26430.0
36-37	39320.0
38-39	48406.0
40-41	49897.0
42-43	60009.0
44-45	71945.0
46-47	70370.0
48-49	64210.0
50-51	56805.0
52-53	56673.0
54-55	63825.0
56-57	68748.0
58-59	69636.0
60-61	69931.0
62-63	68684.0
64-65	70646.0
66-67	77276.0
68-69	69247.0
70-71	68039.0
72-73	64613.0
74-75	66275.0
76-77	66395.0
78-79	71225.0
80-81	72466.0
82-83	70565.0
84-85	69670.0
86-87	74669.0
88-89	80020.0
90-91	83700.0
92-93	87100.0
94-95	104902.0
96-97	295743.0
98-99	550706.0
100-101	5974937.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.23708871525596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63167187153584	73.77442334552485
2	7.734641686350087	12.876181124548783
3	1.9813365151145887	4.947620498501072
4	0.7579959314432655	2.5237349836538465
5	0.34418974156983856	1.4324676026964838
6	0.17273983565769246	0.8627016615178849
7	0.11055374884287314	0.6441520540166851
8	0.07361572119721504	0.49020466529041096
9	0.04547748780071728	0.34068723179537475
>10	0.14427281757867885	1.835840108733679
>50	0.002803583946020384	0.16030250786584918
>100	6.542217846025367E-4	0.0853298436525207
>500	4.6837178520361544E-5	0.026354372202505662
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.235034183171556E-5	0.0	0.0	0.0
3	0.0	2.235034183171556E-5	0.0	1.117517091585778E-5	0.0
4	0.0	8.940136732686224E-5	0.0	2.235034183171556E-5	0.0
5	0.0	8.940136732686224E-5	0.0	2.235034183171556E-5	0.0
6	0.0	8.940136732686224E-5	0.0	2.235034183171556E-5	1.117517091585778E-5
7	0.0	8.940136732686224E-5	0.0	4.470068366343112E-5	1.117517091585778E-5
8	0.0	1.0057653824272001E-4	0.0	4.470068366343112E-5	3.352551274757334E-5
9	0.0	2.3467858923301337E-4	0.0	6.705102549514668E-5	4.470068366343112E-5
10-11	1.117517091585778E-5	2.3467858923301337E-4	0.0	1.173392946165067E-4	5.58758545792889E-5
12-13	1.117517091585778E-5	2.4026617469094226E-4	0.0	1.5645239282200893E-4	6.146344003721778E-5
14-15	1.117517091585778E-5	2.961420292702312E-4	0.0	1.8997790556958227E-4	7.822619641100446E-5
16-17	1.117517091585778E-5	3.0172961472816007E-4	0.0	2.235034183171556E-4	8.940136732686224E-5
18-19	1.117517091585778E-5	3.4643029839159117E-4	0.0	2.570289310647289E-4	1.0616412370064891E-4
20-21	1.117517091585778E-5	3.7995581113916453E-4	0.0	3.184923711019467E-4	1.229268800744356E-4
22-23	3.911309820550223E-5	3.967185675129512E-4	0.0	3.4643029839159117E-4	1.5086480736408003E-4
24-25	5.58758545792889E-5	4.358316657184534E-4	0.0	3.855433965970934E-4	1.8997790556958227E-4
26-27	5.58758545792889E-5	4.358316657184534E-4	0.0	4.2465649480259563E-4	1.8997790556958227E-4
28-29	5.58758545792889E-5	4.470068366343112E-4	0.0	4.972951057556712E-4	1.9556549102751116E-4
30-31	5.58758545792889E-5	5.028826912136001E-4	0.0	9.890026260534134E-4	2.0674066194336892E-4
32-33	6.705102549514668E-5	5.140578621294578E-4	0.0	0.001966830081190969	2.1232824740129782E-4
34-35	6.705102549514668E-5	5.866964730825335E-4	0.0	0.003296675420178045	2.235034183171556E-4
36-37	7.822619641100446E-5	6.59335084035609E-4	0.0	0.0054758337487703125	2.3467858923301337E-4
38-39	7.822619641100446E-5	6.816854258673246E-4	0.0	0.008694282972537354	2.3467858923301337E-4
40-41	7.822619641100446E-5	7.152109386148979E-4	0.0	0.012108297687331904	2.682041019805867E-4
42-43	7.822619641100446E-5	7.487364513624713E-4	0.0	0.01514794417644522	2.682041019805867E-4
44-45	7.822619641100446E-5	8.325502332314046E-4	0.0	0.018723998869519712	2.737916874385156E-4
46-47	7.822619641100446E-5	8.716633314369068E-4	0.0	0.022707947301023008	2.961420292702312E-4
48-49	7.822619641100446E-5	8.772509168948357E-4	0.0	0.026703070903442164	3.0172961472816007E-4
50-51	7.822619641100446E-5	9.722398696796269E-4	0.0	0.030748482774982683	3.0172961472816007E-4
52-53	8.940136732686224E-5	9.834150405954847E-4	0.0	0.034732431206485975	3.408427129336623E-4
54-55	8.940136732686224E-5	0.0010225281388009867	0.0	0.038895182372643	3.7995581113916453E-4
56-57	8.940136732686224E-5	0.0010281157242589157	0.0	0.043443476935397116	3.855433965970934E-4
58-59	8.940136732686224E-5	0.0010560536515485603	0.0	0.04785208186170301	3.967185675129512E-4
60-61	8.940136732686224E-5	0.0010616412370064892	0.0	0.05205953371152347	4.190689093446667E-4
62-63	8.940136732686224E-5	0.0010784039933802757	0.0	0.05584791665199926	4.358316657184534E-4
64-65	8.940136732686224E-5	0.0012292688007443557	0.0	0.05983186508350255	4.414192511763823E-4
66-67	8.940136732686224E-5	0.0012292688007443557	0.0	0.06414548105702367	4.5259442209224013E-4
68-69	8.940136732686224E-5	0.0012627943134919293	0.0	0.06826911912497519	4.58182007550169E-4
70-71	8.940136732686224E-5	0.0013019074116974315	0.0	0.07237040685109497	5.140578621294579E-4
72-73	8.940136732686224E-5	0.001318670168071218	0.0	0.07586264776230053	5.58758545792889E-4
74-75	8.940136732686224E-5	0.0013354329244450046	0.0	0.07881848046954493	5.58758545792889E-4
76-77	8.940136732686224E-5	0.0013521956808187914	0.0	0.08165138629671487	5.58758545792889E-4
78-79	8.940136732686224E-5	0.0013689584371925781	0.0	0.08363497913427961	5.58758545792889E-4
80-81	8.940136732686224E-5	0.0013857211935663647	0.0	0.08419932526553045	5.643461312508178E-4
82-83	9.498895278479112E-5	0.0013857211935663647	0.0	0.08462956934579097	5.811088876246046E-4
84-85	1.0057653824272001E-4	0.0013857211935663647	0.0	0.08484189759319227	5.866964730825335E-4
86-87	1.0057653824272001E-4	0.0014304218772297958	0.0	0.08493688654597706	5.922840585404623E-4
88	1.0057653824272001E-4	0.0015309984154725158	0.0	0.0849648244732667	6.034592294563201E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12840	0.0	43.690895	1
CTTATAC	6955	0.0	34.80666	1
GTATCAA	21720	0.0	33.55508	1
TCAACGC	24880	0.0	28.450472	4
ATCAACG	25120	0.0	28.124872	3
CAACGCA	25345	0.0	27.878155	5
AACGCAG	25955	0.0	27.206417	6
TATCAAC	26365	0.0	27.089808	2
TATACAC	11185	0.0	25.672638	3
TTATACA	10465	0.0	24.15775	2
ACGCAGA	30015	0.0	23.497717	7
CGCAGAG	30160	0.0	23.38514	8
GTACATG	27515	0.0	20.482153	1
TACATGG	26920	0.0	20.185476	2
GAGTACT	19105	0.0	19.929583	12-13
GCAGAGT	35560	0.0	19.811373	9
ACATGGG	27240	0.0	19.333828	3
TACACAT	14040	0.0	19.261936	5
ACATCTC	13015	0.0	18.899235	8
ACACATC	13235	0.0	18.617203	6
>>END_MODULE