FastQCFastQC Report
Fri 27 May 2016
SRR2139112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10274581
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133250.049.285491
GTATCAA237050.036.34711
TCAACGC278050.030.2948914
CTTATAC74100.029.8516161
ATCAACG283450.029.7304423
CAACGCA288650.029.1828125
AACGCAG294150.028.6663596
TATCAAC297700.028.6247442
ACGCAGA340650.024.7785137
CGCAGAG345950.024.4116748
TATACAC114500.023.1450673
GTACATG332950.022.5497231
TTATACA107000.022.4391732
TACATGG333200.022.1718652
ACATGGG338650.021.2062533
GTGGTAT68600.020.5365981
GCAGAGT411000.020.5293739
GAGTACT224200.019.792412-13
CATGGGG244300.018.9368174
TGGTATC65600.018.7913022
Produced by FastQC (version 0.11.5)