##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139112_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10274581
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.618807423874514	33.0	33.0	33.0	33.0	33.0
2	32.625458303360496	33.0	33.0	33.0	33.0	33.0
3	32.631192843776304	33.0	33.0	33.0	33.0	33.0
4	36.545042956009596	37.0	37.0	37.0	37.0	37.0
5	36.57034695624084	37.0	37.0	37.0	37.0	37.0
6	36.57536983746588	37.0	37.0	37.0	37.0	37.0
7	36.550695546611585	37.0	37.0	37.0	37.0	37.0
8	36.54265220158369	37.0	37.0	37.0	37.0	37.0
9	36.57592363133835	37.0	37.0	37.0	37.0	37.0
10-11	36.52761694126505	37.0	37.0	37.0	37.0	37.0
12-13	36.51507871707858	37.0	37.0	37.0	37.0	37.0
14-15	38.61091196808901	40.0	37.0	40.0	37.0	40.0
16-17	38.55020175518593	40.0	37.0	40.0	37.0	40.0
18-19	38.56521385154295	40.0	37.0	40.0	37.0	40.0
20-21	38.56284357172869	40.0	37.0	40.0	37.0	40.0
22-23	38.557202093818674	40.0	37.0	40.0	37.0	40.0
24-25	38.55542931716718	40.0	37.0	40.0	37.0	40.0
26-27	38.436303547499236	40.0	37.0	40.0	37.0	40.0
28-29	38.38781816671403	40.0	37.0	40.0	37.0	40.0
30-31	38.24007377691183	40.0	37.0	40.0	37.0	40.0
32-33	38.254687466279634	40.0	37.0	40.0	37.0	40.0
34-35	38.22605956649214	40.0	37.0	40.0	37.0	40.0
36-37	38.18466693459584	40.0	37.0	40.0	37.0	40.0
38-39	38.130233099887064	40.0	37.0	40.0	37.0	40.0
40-41	38.1058048630063	40.0	37.0	40.0	37.0	40.0
42-43	38.057997759787895	40.0	37.0	40.0	33.0	40.0
44-45	37.98220914680452	40.0	37.0	40.0	33.0	40.0
46-47	37.833564273541725	40.0	37.0	40.0	33.0	40.0
48-49	37.84889452125546	40.0	37.0	40.0	33.0	40.0
50-51	37.81002582692351	40.0	37.0	40.0	33.0	40.0
52-53	37.74972145446635	40.0	37.0	40.0	33.0	40.0
54-55	37.57728139465043	40.0	37.0	40.0	33.0	40.0
56-57	37.45939535569825	40.0	37.0	40.0	33.0	40.0
58-59	37.32958866571957	38.5	37.0	40.0	33.0	40.0
60-61	37.160636763257685	37.0	37.0	40.0	33.0	40.0
62-63	36.99082911602665	37.0	37.0	40.0	33.0	40.0
64-65	36.81549814730813	37.0	37.0	40.0	33.0	40.0
66-67	36.61031287900654	37.0	37.0	40.0	33.0	40.0
68-69	36.399230674758954	37.0	37.0	40.0	33.0	40.0
70-71	36.16322555657041	37.0	37.0	38.5	33.0	40.0
72-73	35.937395476606795	37.0	37.0	37.0	33.0	40.0
74-75	35.74289846337053	37.0	35.0	37.0	33.0	40.0
76-77	34.06389518284463	35.0	33.0	37.0	30.0	37.0
78-79	35.24834961417511	37.0	33.0	37.0	33.0	37.0
80-81	35.308789990738916	37.0	33.0	37.0	33.0	37.0
82-83	35.16943092707319	37.0	33.0	37.0	33.0	37.0
84-85	35.04029313764643	37.0	33.0	37.0	33.0	37.0
86-87	34.95472025892933	37.0	33.0	37.0	33.0	37.0
88-89	34.82169125123424	37.0	33.0	37.0	33.0	37.0
90-91	34.675812808962235	37.0	33.0	37.0	33.0	37.0
92-93	34.674906358471944	37.0	33.0	37.0	33.0	37.0
94-95	34.735983671125446	37.0	33.0	37.0	33.0	37.0
96-97	34.743079148255674	37.0	33.0	37.0	33.0	37.0
98-99	34.74390613641843	37.0	33.0	37.0	33.0	37.0
100	34.733900714723184	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	15.0
20	76.0
21	392.0
22	1343.0
23	4075.0
24	9184.0
25	18097.0
26	31316.0
27	49628.0
28	71891.0
29	101226.0
30	137882.0
31	182668.0
32	242555.0
33	325538.0
34	453100.0
35	664005.0
36	1183379.0
37	2980667.0
38	3717196.0
39	100342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.606319518041666	19.92025757546707	14.360906785395922	24.11251612109535
2	14.622679017275741	20.517128630354854	42.006812735234654	22.853379617134753
3	18.474154809816575	25.772447557715495	30.023287567639013	25.73011006482892
4	12.599988262295076	17.91845331697711	38.078224309098346	31.403334111629466
5	13.706661128079093	37.467805256486855	35.11591372923139	13.70961988620266
6	30.42917822514352	37.221309396426086	18.61460620331798	13.734906175112407
7	26.94816460155407	32.25484328752676	22.53851519589947	18.2584769150197
8	24.537672144489395	36.90369466161199	20.782706370215973	17.77592682368264
9	25.637846023876403	16.879551280928272	20.67805204689632	36.804550648299006
10-11	24.592053327246727	26.551339082030257	28.39211119219558	20.464496398527437
12-13	25.411902521748093	23.917885102640216	28.515450608730536	22.154761766881155
14-15	22.482125290043978	25.35026469569947	26.031740902384136	26.135869111872417
16-17	22.190101961335454	28.537616278464302	26.703828603813623	22.56845315638662
18-19	22.231519708686903	27.568048760333873	28.42173320741741	21.778698323561805
20-21	22.951091397721783	26.68522615741651	28.303800883520786	22.059881561340923
22-23	22.70484503028144	26.7825849116475	28.17067401753228	22.341896040538778
24-25	22.517039353336948	26.968246102682976	28.233680334819276	22.2810342091608
26-27	22.38548366575944	27.03803391871562	28.270320985062288	22.30616143046265
28-29	22.22677173009845	27.006536337391896	28.393434816584932	22.373257115924723
30-31	22.565866207631842	26.864720497469722	28.068843523287267	22.50056977161116
32-33	22.466787331244774	26.973680130460338	28.273551628300357	22.285980909994528
34-35	22.54415652071355	27.167035866230755	28.142246356473784	22.14656125658191
36-37	22.47188087605002	27.071128741647726	28.004772027669905	22.452218354632354
38-39	22.364924432534995	27.0546065183665	28.161313841755437	22.41915520734307
40-41	22.526254103203115	26.940447261164298	28.12291951399549	22.410379121637096
42-43	22.546673913548716	27.004967651861595	27.970693816473872	22.477664618115814
44-45	22.489115453544535	27.16805900458889	27.78723750512332	22.555588036743256
46-47	22.766051519396253	27.086795130846387	27.396775028134527	22.75037832162283
48-49	22.597953106759007	27.216010928958962	27.416013817787498	22.770022146494536
50-51	22.554547808022313	27.37937445033744	27.399957558905015	22.666120182735234
52-53	22.686902766572786	27.322062089793143	27.419280976605837	22.571754167028235
54-55	22.6228207788159	27.282099762890066	27.297520869550358	22.797558588743676
56-57	22.726340773301278	27.46413749235076	27.14112487106876	22.668396863279202
58-59	22.58828913708821	27.55996507854284	27.310705317412765	22.541040466956183
60-61	22.766119622388924	27.63730462640822	27.102054912242686	22.49452083896017
62-63	22.630045716975904	27.763566190032567	27.058915100974225	22.547472992017305
64-65	22.648236424018794	27.656841835847974	27.036852576392274	22.65806916374096
66-67	22.711255958841498	27.520876943719465	27.00632988089524	22.761537216543797
68-69	22.695352692146102	27.461054272901524	27.08650409990837	22.757088935044003
70-71	22.674459104492943	27.316705050218637	27.14997940349393	22.858856441794497
72-73	22.751840751332406	27.323290462237992	27.06624632677455	22.858622459655052
74-75	22.64227586411335	27.43988002639074	27.058607866816935	22.85923624267897
76-77	22.750773461906995	27.375762305242446	26.999546400668105	22.873917832182457
78-79	22.69241034352335	27.395655150451265	26.983418807163883	22.928515698861503
80-81	22.710611699361277	27.442196108353627	26.999427620091502	22.84776457219359
82-83	22.72911646852678	27.437344616033045	27.019572300795357	22.813966614644816
84-85	22.787212771158472	27.39439133385675	27.029349911288588	22.789045983696194
86-87	22.71350335018595	27.376594321243974	27.099803503043628	22.810098825526453
88-89	22.765018480989355	27.339913195399056	27.030607629168703	22.864460694442883
90-91	22.798822124179278	27.40311279244051	26.988436871796285	22.80962821158393
92-93	22.737705946198325	27.36315321707754	27.099164779109095	22.799976057615034
94-95	22.677748921111025	27.395477725066975	27.098183593373243	22.82858976044876
96-97	22.77979075393761	27.49011001712947	27.123902116380883	22.606197112552035
98-99	23.896864656882215	29.42760727076248	27.293078459995385	19.38244961235992
100	22.72557479709933	30.443888008954566	21.09895636499789	25.731580828948207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1961.0
1	1762.5
2	2327.5
3	3281.5
4	4442.0
5	7253.0
6	9652.5
7	11147.5
8	11989.5
9	11841.5
10	11071.0
11	10089.5
12	10873.0
13	14463.0
14	22874.5
15	34648.0
16	44658.5
17	50944.5
18	52515.0
19	49693.5
20	44567.5
21	38923.0
22	35108.5
23	35446.0
24	40194.0
25	49100.5
26	61752.0
27	76940.0
28	94819.0
29	115858.5
30	138566.5
31	161755.0
32	186516.5
33	211996.0
34	235979.0
35	258682.5
36	283008.5
37	307296.0
38	328543.5
39	345293.5
40	362839.5
41	379602.5
42	391121.0
43	402536.5
44	413254.0
45	423461.0
46	430611.5
47	435814.0
48	447321.0
49	447720.5
50	435403.0
51	426755.5
52	415368.0
53	393335.5
54	367815.5
55	343030.0
56	316170.5
57	287521.0
58	255370.0
59	221016.5
60	185712.0
61	152944.5
62	122539.5
63	94928.0
64	71181.5
65	53022.0
66	40608.0
67	31500.0
68	24073.5
69	17370.0
70	12314.0
71	8897.5
72	6351.5
73	4377.5
74	2880.5
75	1906.5
76	1235.0
77	729.0
78	464.5
79	317.5
80	211.0
81	144.0
82	104.5
83	73.5
84	48.5
85	34.0
86	28.5
87	22.0
88	14.5
89	8.5
90	5.5
91	4.5
92	4.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.4599135478128014E-4
7	0.0
8	0.0
9	9.732756985418675E-6
10-11	0.006409020474898198
12-13	1.946551397083735E-5
14-15	0.006890791945676422
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	133.0
20-21	587.0
22-23	1155.0
24-25	2968.0
26-27	3629.0
28-29	6192.0
30-31	16146.0
32-33	20413.0
34-35	28989.0
36-37	43788.0
38-39	53756.0
40-41	55743.0
42-43	67096.0
44-45	80572.0
46-47	78535.0
48-49	72077.0
50-51	64489.0
52-53	63919.0
54-55	71859.0
56-57	77351.0
58-59	77845.0
60-61	79662.0
62-63	79073.0
64-65	82216.0
66-67	88681.0
68-69	79889.0
70-71	79224.0
72-73	75274.0
74-75	76518.0
76-77	76859.0
78-79	82745.0
80-81	84562.0
82-83	81893.0
84-85	81447.0
86-87	86881.0
88-89	93532.0
90-91	98161.0
92-93	101000.0
94-95	121767.0
96-97	345828.0
98-99	636186.0
100-101	6855941.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.93683286447364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.04205654788319	74.7390821836868
2	7.622202241254995	12.795670311668738
3	1.8460510087470787	4.648550249414896
4	0.6630589489536582	2.226202727104671
5	0.3094534661221985	1.2987271932615516
6	0.17537724512275646	0.8832366307260382
7	0.09757522319872329	0.5733108640941463
8	0.06210826383984977	0.4170536769142481
9	0.039085401360145856	0.295263432126669
>10	0.1387794533624892	1.8223888615510422
>50	0.0033999931542280965	0.19216814875372637
>100	8.522070006404586E-4	0.10834572069744915
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.9198270956256026E-5	0.0	0.0	0.0
5	0.0	2.9198270956256026E-5	0.0	0.0	0.0
6	4.866378492709338E-5	2.9198270956256026E-5	0.0	0.0	0.0
7	4.866378492709338E-5	2.9198270956256026E-5	0.0	0.0	0.0
8	4.866378492709338E-5	2.9198270956256026E-5	0.0	0.0	0.0
9	4.866378492709338E-5	8.759481286876808E-5	0.0	0.0	9.732756985418675E-6
10-11	5.353016341980271E-5	8.759481286876808E-5	0.0	0.0	9.732756985418675E-6
12-13	5.839654191251205E-5	8.759481286876808E-5	0.0	0.0	9.732756985418675E-6
14-15	5.839654191251205E-5	1.3625859779586145E-4	0.0	0.0	1.4599135478128013E-5
16-17	1.3625859779586145E-4	1.557241117666988E-4	0.0	0.0	1.946551397083735E-5
18-19	1.3625859779586145E-4	1.800560042302455E-4	0.0	0.0	3.406464944896536E-5
20-21	1.4599135478128014E-4	2.0438789669379218E-4	0.0	0.0	4.866378492709338E-5
22-23	1.4599135478128014E-4	2.4818530312817626E-4	0.0	0.0	6.326292040522139E-5
24-25	1.4599135478128014E-4	2.919827095625603E-4	0.0	0.0	7.78620558833494E-5
26-27	1.4599135478128014E-4	3.1631460202610697E-4	0.0	2.433189246354669E-5	7.78620558833494E-5
28-29	1.4599135478128014E-4	3.2118098051881627E-4	0.0	8.272843437605873E-5	7.78620558833494E-5
30-31	1.4599135478128014E-4	3.60112008460491E-4	0.0	5.158361202271897E-4	7.78620558833494E-5
32-33	1.4599135478128014E-4	3.6984476544590965E-4	0.0	0.0016740342014920121	7.78620558833494E-5
34-35	1.4599135478128014E-4	4.525731998219684E-4	0.0	0.003022021043972499	7.78620558833494E-5
36-37	1.4599135478128014E-4	5.304352557053178E-4	0.0	0.005333550828009435	7.78620558833494E-5
38-39	1.4599135478128014E-4	5.547671481688645E-4	0.0	0.00867188647400804	7.78620558833494E-5
40-41	1.4599135478128014E-4	5.985645546032485E-4	0.0	0.012321670343540043	7.78620558833494E-5
42-43	1.4599135478128014E-4	6.18030068574086E-4	0.0	0.015898458535681406	7.78620558833494E-5
44-45	1.4599135478128014E-4	6.812929889793073E-4	0.0	0.019937552684630155	7.78620558833494E-5
46-47	1.4599135478128014E-4	7.20224016920982E-4	0.0	0.024365957112995655	8.759481286876808E-5
48-49	1.5085773327398947E-4	7.299567739064007E-4	0.0	0.029135008035850805	8.759481286876808E-5
50-51	1.7518962573753616E-4	8.516162362241341E-4	0.0	0.0338748606877497	8.759481286876808E-5
52-53	1.8492238272295483E-4	8.662153717022621E-4	0.0	0.03840545906446209	8.759481286876808E-5
54-55	1.8492238272295483E-4	8.856808856730994E-4	0.0	0.04322804015073705	9.246119136147743E-5
56-57	1.8492238272295483E-4	9.051463996439368E-4	0.0	0.0478413669618255	1.1192670533231477E-4
58-59	1.8492238272295483E-4	9.051463996439368E-4	0.0	0.05290240059424321	1.2652584081044278E-4
60-61	2.1412065367921085E-4	9.392110490929022E-4	0.0	0.058002365254602596	1.3625859779586145E-4
62-63	2.627844386063042E-4	9.440774275856115E-4	0.0	0.06276168342047234	1.4599135478128014E-4
64-65	2.627844386063042E-4	0.0010900687823668916	0.0	0.06746747142292225	1.4599135478128014E-4
66-67	2.676508170990136E-4	0.0010998015393523103	0.0	0.07246037575644204	1.4599135478128014E-4
68-69	2.725171955917229E-4	0.0011241334318158572	0.0	0.07753600852433787	1.4599135478128014E-4
70-71	2.8224995257714156E-4	0.0011435989457866944	0.0	0.08185735262586377	1.4599135478128014E-4
72-73	3.2118098051881627E-4	0.0011581980812648224	0.0	0.08565799422866976	1.557241117666988E-4
74-75	3.2118098051881627E-4	0.001167930838250241	0.0	0.08882114024893084	1.557241117666988E-4
76-77	3.2118098051881627E-4	0.0012506592726263	0.0	0.09156091134032619	1.6059049025940816E-4
78-79	3.2118098051881627E-4	0.0012749911650898466	0.0	0.09329820846222342	1.654568687521175E-4
80-81	3.2118098051881627E-4	0.0012749911650898466	0.0	0.09390163939531938	1.654568687521175E-4
82-83	3.2118098051881627E-4	0.0012895903005679743	0.0	0.09430068243172154	1.654568687521175E-4
84-85	3.4064649448965366E-4	0.0012944566790606838	0.0	0.09448073843595178	1.654568687521175E-4
86-87	3.45512872982363E-4	0.0012944566790606838	0.0	0.09452940222087888	1.654568687521175E-4
88	3.503792514750723E-4	0.0014015170059002893	0.0	0.0945342685993716	1.654568687521175E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13325	0.0	49.28549	1
GTATCAA	23705	0.0	36.3471	1
TCAACGC	27805	0.0	30.294891	4
CTTATAC	7410	0.0	29.851616	1
ATCAACG	28345	0.0	29.730442	3
CAACGCA	28865	0.0	29.182812	5
AACGCAG	29415	0.0	28.666359	6
TATCAAC	29770	0.0	28.624744	2
ACGCAGA	34065	0.0	24.778513	7
CGCAGAG	34595	0.0	24.411674	8
TATACAC	11450	0.0	23.145067	3
GTACATG	33295	0.0	22.549723	1
TTATACA	10700	0.0	22.439173	2
TACATGG	33320	0.0	22.171865	2
ACATGGG	33865	0.0	21.206253	3
GTGGTAT	6860	0.0	20.536598	1
GCAGAGT	41100	0.0	20.529373	9
GAGTACT	22420	0.0	19.7924	12-13
CATGGGG	24430	0.0	18.936817	4
TGGTATC	6560	0.0	18.791302	2
>>END_MODULE