FastQCFastQC Report
Fri 27 May 2016
SRR2139111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10895216
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137450.047.927711
GTATCAA246000.037.256391
TCAACGC295550.030.2849244
ATCAACG299000.029.9472623
CAACGCA305800.029.270895
TATCAAC309800.029.1532692
AACGCAG311950.028.7214136
CTTATAC74050.025.2767051
ACGCAGA361350.024.8424427
CGCAGAG368650.024.3160518
GTACATG342850.022.1386261
TACATGG343850.021.436042
TATACAC108900.020.9413283
GCAGAGT432350.020.6471029
ACATGGG348250.020.6092013
GAGTACT237750.019.7938512-13
CATGGGG260650.018.8704664
GTGGTAT72200.018.6536311
AGAGTAC386750.018.48443610-11
CAGAGTA418350.018.48222710-11
Produced by FastQC (version 0.11.5)