##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139111_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10895216
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.619279691196574	33.0	33.0	33.0	33.0	33.0
2	32.62709082591846	33.0	33.0	33.0	33.0	33.0
3	32.633609650327266	33.0	33.0	33.0	33.0	33.0
4	36.55045113378202	37.0	37.0	37.0	37.0	37.0
5	36.57370684527962	37.0	37.0	37.0	37.0	37.0
6	36.57917181265612	37.0	37.0	37.0	37.0	37.0
7	36.554986427070375	37.0	37.0	37.0	37.0	37.0
8	36.53868798929732	37.0	37.0	37.0	37.0	37.0
9	36.57492884950606	37.0	37.0	37.0	37.0	37.0
10-11	36.5293939101345	37.0	37.0	37.0	37.0	37.0
12-13	36.51683835364072	37.0	37.0	37.0	37.0	37.0
14-15	38.616215272831674	40.0	37.0	40.0	37.0	40.0
16-17	38.55674086681714	40.0	37.0	40.0	37.0	40.0
18-19	38.57282063063275	40.0	37.0	40.0	37.0	40.0
20-21	38.56695320341116	40.0	37.0	40.0	37.0	40.0
22-23	38.56517333109424	40.0	37.0	40.0	37.0	40.0
24-25	38.56198093915607	40.0	37.0	40.0	37.0	40.0
26-27	38.439399169859925	40.0	37.0	40.0	37.0	40.0
28-29	38.3907916273613	40.0	37.0	40.0	37.0	40.0
30-31	38.23375489036755	40.0	37.0	40.0	37.0	40.0
32-33	38.25275000534374	40.0	37.0	40.0	37.0	40.0
34-35	38.229417369524796	40.0	37.0	40.0	37.0	40.0
36-37	38.18466990699174	40.0	37.0	40.0	37.0	40.0
38-39	38.13199757332619	40.0	37.0	40.0	37.0	40.0
40-41	38.10784011356006	40.0	37.0	40.0	37.0	40.0
42-43	38.06080659368352	40.0	37.0	40.0	33.0	40.0
44-45	37.986786909027316	40.0	37.0	40.0	33.0	40.0
46-47	37.839075001079664	40.0	37.0	40.0	33.0	40.0
48-49	37.85157956970089	40.0	37.0	40.0	33.0	40.0
50-51	37.81083371457245	40.0	37.0	40.0	33.0	40.0
52-53	37.74437432464774	40.0	37.0	40.0	33.0	40.0
54-55	37.57927302872531	40.0	37.0	40.0	33.0	40.0
56-57	37.461776517413526	40.0	37.0	40.0	33.0	40.0
58-59	37.33222328188073	38.5	37.0	40.0	33.0	40.0
60-61	37.163151246189685	37.0	37.0	40.0	33.0	40.0
62-63	36.99168243824566	37.0	37.0	40.0	33.0	40.0
64-65	36.813644332420935	37.0	37.0	40.0	33.0	40.0
66-67	36.61094539819521	37.0	37.0	40.0	33.0	40.0
68-69	36.39389478641568	37.0	37.0	40.0	33.0	40.0
70-71	36.16020109828838	37.0	37.0	38.5	33.0	40.0
72-73	35.93489562202815	37.0	37.0	37.0	33.0	40.0
74-75	35.743511495729315	37.0	35.0	37.0	33.0	40.0
76-77	34.07339923448106	35.0	33.0	37.0	30.0	37.0
78-79	35.24358784664673	37.0	33.0	37.0	33.0	37.0
80-81	35.304571504324414	37.0	33.0	37.0	33.0	37.0
82-83	35.16670090107701	37.0	33.0	37.0	33.0	37.0
84-85	35.035139176371985	37.0	33.0	37.0	33.0	37.0
86-87	34.95316044013286	37.0	33.0	37.0	33.0	37.0
88-89	34.821074901971905	37.0	33.0	37.0	33.0	37.0
90-91	34.675010633599726	37.0	33.0	37.0	33.0	37.0
92-93	34.67223248441651	37.0	33.0	37.0	33.0	37.0
94-95	34.73621074342049	37.0	33.0	37.0	33.0	37.0
96-97	34.74321203918602	37.0	33.0	37.0	33.0	37.0
98-99	34.74337953584396	37.0	33.0	37.0	33.0	37.0
100	34.7289872932447	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	2.0
18	6.0
19	20.0
20	76.0
21	385.0
22	1508.0
23	4395.0
24	9860.0
25	19010.0
26	33208.0
27	52656.0
28	77355.0
29	107347.0
30	146098.0
31	194725.0
32	258284.0
33	346901.0
34	481218.0
35	702668.0
36	1250161.0
37	3155608.0
38	3947265.0
39	106458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80163110120992	19.830483397483814	14.249391659605465	24.118493841700797
2	14.672035873359462	20.54196080187855	41.90724626294697	22.87875706181502
3	18.392769817505226	25.830979394993182	30.047059186343805	25.72919160115779
4	12.633223609334593	17.84290462896743	38.22525409317264	31.298617668525342
5	13.635424942470161	37.526002238046495	35.212188542200536	13.6263842772828
6	30.427223624676735	37.407160815273976	18.54000813570473	13.625607424344555
7	26.905864004899033	32.32608697248407	22.654208966577624	18.113840056039272
8	24.516466676750603	36.84395059262708	20.842927758384967	17.796654972237356
9	25.663880887160097	16.81109551303026	20.708283406981874	36.816740192827766
10-11	24.600444563179018	26.522416395884367	28.47632173053696	20.400817310399653
12-13	25.462027473075903	23.940427963199433	28.53552420386477	22.062020359859893
14-15	22.490542685068775	25.35325468124812	26.145523093949347	26.010679539733754
16-17	22.193791293352973	28.457343112793726	26.761736527297852	22.587129066555452
18-19	22.224442360757234	27.5621382816091	28.44507626099382	21.768343096639846
20-21	23.036752052968364	26.70117713187644	28.35222097271435	21.909849842440853
22-23	22.6951733115811	26.79568231138591	28.243763647446258	22.265380729586735
24-25	22.503472872211063	26.93924716044325	28.32156092924889	22.235719038096796
26-27	22.497344220479636	27.076989481345258	28.206024664827652	22.219641633347454
28-29	22.290026408582932	27.11366164242561	28.316616717332614	22.27969523165884
30-31	22.818525902605533	26.935272588976904	28.076613127914996	22.16958838050257
32-33	22.287842588001684	27.258612544441004	28.137641548267084	22.315903319290225
34-35	22.50058548890331	27.258147850048747	28.160036156766832	22.08123050428111
36-37	22.453573847518445	27.18210024987031	28.052358199129575	22.31196770348167
38-39	22.33637434476103	27.109224447861347	28.19740912619837	22.356992081179257
40-41	22.5999209132739	26.956666612395132	28.099545597539887	22.343866876791076
42-43	22.522436160340597	27.028393559107457	27.95845753159482	22.49071274895713
44-45	22.534335712345044	27.230918342254235	27.71258242059307	22.522163524807656
46-47	22.758007416784846	27.132172703494163	27.337182875860428	22.772637003860567
48-49	22.591946758895958	27.250885387898606	27.416762843218777	22.74040500998666
50-51	22.58084424078181	27.405318482487402	27.345986064135097	22.667851212595686
52-53	22.749163294539322	27.33652298268044	27.321696187340365	22.592617535439874
54-55	22.675750974834035	27.258032630963157	27.274827018058733	22.791389376144075
56-57	22.76706939010445	27.46474446532872	27.116101102810514	22.652085041756315
58-59	22.611249417961215	27.562216363750203	27.24461266498422	22.58192155330437
60-61	22.753415108035963	27.59997138860263	27.11006412949748	22.53654937386393
62-63	22.686799070679392	27.700905899310136	27.042942482516626	22.56935254749385
64-65	22.678501447811378	27.593236611998062	27.031462976926967	22.696798963263593
66-67	22.756236438666857	27.537553835566136	26.97004474417178	22.736164981595227
68-69	22.701145593209496	27.47388977225868	27.07316968986115	22.751794944670674
70-71	22.724220440516117	27.279100814380108	27.108355730783284	22.888323014320495
72-73	22.756541365316153	27.332105924274487	27.023235836245263	22.8881168741641
74-75	22.60946365737296	27.479733624820174	27.026044152387403	22.884758565419467
76-77	22.766121718729046	27.371797114997996	26.98911730706259	22.872963859210365
78-79	22.73327541892378	27.41477300547278	26.928932839232893	22.923018736370544
80-81	22.760449933633602	27.451250195276067	26.928772739087663	22.859527132002665
82-83	22.793250347193233	27.371424783397742	27.009284281292672	22.826040588116353
84-85	22.839876778268938	27.358942299199118	26.977793752085354	22.82338717044659
86-87	22.687360675135917	27.378873869642067	27.076860634723825	22.85690482049819
88-89	22.808627598435162	27.35685838377234	26.990051749059447	22.844462268733047
90-91	22.855048502354183	27.38230110611654	26.971565866735315	22.791084524793963
92-93	22.77981580824168	27.389250787071482	27.035643625314997	22.79528977937184
94-95	22.693786752455498	27.421585868084254	27.041559993881258	22.843067385578987
96-97	22.80500774632816	27.491904640212404	27.09524046467341	22.60784714878602
98-99	23.937358568411266	29.459231313752205	27.229337505903345	19.374072611933187
100	22.800639758066296	30.441017214777155	21.085768508075034	25.672574519081515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2047.0
1	1870.5
2	2552.5
3	3629.0
4	4969.0
5	8319.5
6	11180.5
7	12851.5
8	13687.0
9	13225.0
10	12129.0
11	11044.5
12	11969.5
13	15904.0
14	25317.0
15	38745.0
16	50187.0
17	57346.0
18	59333.5
19	56264.0
20	50281.0
21	43602.0
22	39064.5
23	39001.0
24	43739.0
25	53249.5
26	67192.0
27	83945.0
28	102520.5
29	124376.5
30	147203.0
31	171024.0
32	198048.5
33	225339.0
34	251073.5
35	275414.5
36	302566.5
37	328457.5
38	349132.0
39	367903.0
40	387192.0
41	402296.0
42	411769.0
43	422218.5
44	431826.5
45	442504.5
46	453241.0
47	462639.0
48	466542.0
49	462239.0
50	455951.5
51	445176.5
52	427938.0
53	407305.0
54	386672.5
55	363298.5
56	337414.0
57	309070.0
58	276016.5
59	239932.5
60	201748.5
61	165684.5
62	132186.5
63	102108.0
64	76903.5
65	56875.0
66	42467.5
67	32499.0
68	24939.5
69	18203.0
70	13038.5
71	9351.5
72	6606.0
73	4558.0
74	3091.5
75	2047.0
76	1273.5
77	761.5
78	483.0
79	322.5
80	212.5
81	141.5
82	98.0
83	65.0
84	48.0
85	36.0
86	28.0
87	25.5
88	22.0
89	18.0
90	13.5
91	9.5
92	7.0
93	5.0
94	3.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.937068893356497E-4
7	0.0
8	0.0
9	9.178340291739053E-6
10-11	0.0064386057146549455
12-13	3.2124191021086684E-5
14-15	0.00678279347559516
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	156.0
20-21	575.0
22-23	1305.0
24-25	3268.0
26-27	3937.0
28-29	6852.0
30-31	17356.0
32-33	21913.0
34-35	31611.0
36-37	47959.0
38-39	58626.0
40-41	60121.0
42-43	71993.0
44-45	86627.0
46-47	83862.0
48-49	76045.0
50-51	68500.0
52-53	66729.0
54-55	73583.0
56-57	79882.0
58-59	80635.0
60-61	82092.0
62-63	79787.0
64-65	82776.0
66-67	89673.0
68-69	83814.0
70-71	82893.0
72-73	79415.0
74-75	80541.0
76-77	80516.0
78-79	87243.0
80-81	89621.0
82-83	87087.0
84-85	85646.0
86-87	91384.0
88-89	98361.0
90-91	103148.0
92-93	107134.0
94-95	128281.0
96-97	365641.0
98-99	672745.0
100-101	7295883.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.72116089647993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.73486579262183	73.40251110359024
2	7.571259684201322	12.526067810516986
3	1.9238343898629071	4.774254423060925
4	0.7776976819165798	2.5732822029856344
5	0.3610763617955281	1.493432791000174
6	0.1941179934597616	0.9634599461932066
7	0.1260323283237968	0.7297878356601546
8	0.07530786772590717	0.49836433943404757
9	0.05625560905233513	0.4188176358972918
>10	0.17494253734465828	2.2529966894265376
>50	0.003413166644020868	0.1844127319663745
>100	0.0011350663121645573	0.12929694212369844
>500	2.050833253774948E-5	0.00900827203129629
>1k	4.10124066098563E-5	0.04430727611343339
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.178340291739053E-6	0.0	0.0	0.0
3	0.0	9.178340291739053E-6	0.0	9.178340291739053E-6	0.0
4	0.0	6.424838204217337E-5	0.0	9.178340291739053E-6	0.0
5	0.0	6.424838204217337E-5	0.0	9.178340291739053E-6	0.0
6	0.0	6.424838204217337E-5	0.0	9.178340291739053E-6	0.0
7	0.0	6.424838204217337E-5	0.0	9.178340291739053E-6	0.0
8	0.0	7.342672233391242E-5	0.0	9.178340291739053E-6	0.0
9	0.0	1.376751043760858E-4	0.0	1.8356680583478106E-5	0.0
10-11	0.0	1.376751043760858E-4	0.0	2.7535020875217157E-5	0.0
12-13	0.0	1.376751043760858E-4	0.0	3.671336116695621E-5	0.0
14-15	0.0	2.2028016700173728E-4	0.0	5.048087160456479E-5	9.178340291739053E-6
16-17	4.589170145869526E-6	2.2028016700173728E-4	0.0	5.507004175043432E-5	9.178340291739053E-6
18-19	9.178340291739053E-6	2.4322601773108492E-4	0.0	5.965921189630384E-5	1.3767510437608578E-5
20-21	9.178340291739053E-6	2.661718684604325E-4	0.0	9.637257306326006E-5	1.8356680583478106E-5
22-23	9.178340291739053E-6	2.8452854904391063E-4	0.0	1.1014008350086864E-4	1.8356680583478106E-5
24-25	1.8356680583478106E-5	3.625444415236926E-4	0.0	1.193184237926077E-4	2.2945850729347633E-5
26-27	2.753502087521716E-5	3.9007946239890973E-4	0.0	1.376751043760858E-4	2.753502087521716E-5
28-29	2.753502087521716E-5	3.946686325447793E-4	0.0	2.4322601773108492E-4	2.753502087521716E-5
30-31	2.753502087521716E-5	4.818628653163003E-4	0.0	6.287163099841251E-4	2.753502087521716E-5
32-33	2.753502087521716E-5	5.231653966291259E-4	0.0	0.0017897763568891153	2.753502087521716E-5
34-35	2.753502087521716E-5	6.241271398382557E-4	0.0	0.0032904349945884507	2.753502087521716E-5
36-37	2.753502087521716E-5	7.159105427556461E-4	0.0	0.005507004175043432	2.753502087521716E-5
38-39	2.753502087521716E-5	7.250888830473852E-4	0.0	0.009325193736406879	3.671336116695621E-5
40-41	2.753502087521716E-5	7.84748094943689E-4	0.0	0.013620656992940756	3.671336116695621E-5
42-43	2.753502087521716E-5	8.122831158189061E-4	0.0	0.017783034315244417	4.130253131282574E-5
44-45	2.753502087521716E-5	8.81120668006949E-4	0.0	0.022436452843156117	4.5891701458695266E-5
46-47	2.753502087521716E-5	9.40779879903253E-4	0.0	0.02754878838565477	4.5891701458695266E-5
48-49	2.753502087521716E-5	9.453690500491224E-4	0.0	0.03274372899077907	4.5891701458695266E-5
50-51	2.753502087521716E-5	0.0010738658141334692	0.0	0.03796161544663272	4.5891701458695266E-5
52-53	3.2124191021086684E-5	0.0011014008350086863	0.0	0.04332176617700833	4.5891701458695266E-5
54-55	3.671336116695621E-5	0.0011059900051545559	0.0	0.04783292043039808	5.507004175043432E-5
56-57	3.671336116695621E-5	0.0011289358558839036	0.0	0.052201810409265866	6.88375521880429E-5
58-59	3.671336116695621E-5	0.0011335250260297732	0.0	0.05692865565951148	8.260506262565148E-5
60-61	3.671336116695621E-5	0.0011472925364673816	0.0	0.061852835226029475	8.260506262565148E-5
62-63	3.671336116695621E-5	0.0011931842379260768	0.0	0.06664851802846314	9.178340291739053E-5
64-65	3.671336116695621E-5	0.0013400376825939018	0.0	0.07136159576827114	9.178340291739053E-5
66-67	3.671336116695621E-5	0.0013446268527397714	0.0	0.07643721794960284	9.178340291739053E-5
68-69	3.671336116695621E-5	0.001367572703469119	0.0	0.08147612676976757	9.637257306326006E-5
70-71	3.671336116695621E-5	0.0013813402139067274	0.0	0.08587255176951059	1.0096174320912959E-4
72-73	3.671336116695621E-5	0.001395107724344336	0.0	0.08918134344468251	1.0096174320912959E-4
74-75	3.671336116695621E-5	0.0014226427452195532	0.0	0.09188436466059965	1.0096174320912959E-4
76-77	3.671336116695621E-5	0.0014501777660947705	0.0	0.09461033172724617	1.0096174320912959E-4
78-79	3.671336116695621E-5	0.0014685344466782485	0.0	0.09614311455596658	1.0096174320912959E-4
80-81	3.671336116695621E-5	0.0014685344466782485	0.0	0.09670758248390854	1.1014008350086864E-4
82-83	3.671336116695621E-5	0.0014685344466782485	0.0	0.09704259190455701	1.193184237926077E-4
84-85	3.671336116695621E-5	0.0014777127869699876	0.0	0.09717567783878722	1.193184237926077E-4
86-87	3.671336116695621E-5	0.0014914802974075963	0.0	0.09726746124170461	1.239075939384772E-4
88	3.671336116695621E-5	0.0015970312107625952	0.0	0.09727205041185048	1.2849676408434674E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13745	0.0	47.92771	1
GTATCAA	24600	0.0	37.25639	1
TCAACGC	29555	0.0	30.284924	4
ATCAACG	29900	0.0	29.947262	3
CAACGCA	30580	0.0	29.27089	5
TATCAAC	30980	0.0	29.153269	2
AACGCAG	31195	0.0	28.721413	6
CTTATAC	7405	0.0	25.276705	1
ACGCAGA	36135	0.0	24.842442	7
CGCAGAG	36865	0.0	24.316051	8
GTACATG	34285	0.0	22.138626	1
TACATGG	34385	0.0	21.43604	2
TATACAC	10890	0.0	20.941328	3
GCAGAGT	43235	0.0	20.647102	9
ACATGGG	34825	0.0	20.609201	3
GAGTACT	23775	0.0	19.79385	12-13
CATGGGG	26065	0.0	18.870466	4
GTGGTAT	7220	0.0	18.653631	1
AGAGTAC	38675	0.0	18.484436	10-11
CAGAGTA	41835	0.0	18.482227	10-11
>>END_MODULE