FastQCFastQC Report
Fri 27 May 2016
SRR2139110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8588036
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113400.045.2149621
GTATCAA194350.035.5076751
TCAACGC233650.029.0962074
ATCAACG236550.028.6648773
CAACGCA239700.028.3088995
TATCAAC246500.027.6820242
AACGCAG245100.027.6326336
ACGCAGA279850.024.2167347
CGCAGAG284900.023.7880338
GTACATG293950.023.0966661
TACATGG292300.022.536552
ACATGGG295300.021.6677463
GCAGAGT343600.019.6522249
GAGTACT175950.018.83284612-13
CATGGGG206200.018.7445534
GTGGTAT62500.018.620021
AGAGTAC305400.017.91129710-11
CAGAGTA329900.017.78547510-11
TGGTATC61050.017.3740942
GTACTTT196400.016.85041214-15
Produced by FastQC (version 0.11.5)