FastQCFastQC Report
Fri 27 May 2016
SRR2139109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4950550
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65000.047.996571
GTATCAA110750.036.741191
TCAACGC133950.029.543084
ATCAACG136100.029.1685583
CAACGCA139400.028.3889165
TATCAAC142800.028.2532672
AACGCAG142900.027.6633976
ACGCAGA161600.024.4352997
CGCAGAG167100.023.6575688
GTGGTAT32050.022.2147371
TACATGG149450.021.6834952
GTACATG152450.021.6530591
TGGTATC31850.020.7284722
ACATGGG155150.020.3862553
GCAGAGT194100.020.258829
GAGTACT110950.018.72980312-13
CAGAGTA189250.018.41645210-11
AGAGTAC175450.018.25365610-11
CATGGGG116350.018.1001684
GTACTTT121150.017.13584914-15
Produced by FastQC (version 0.11.5)