##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4950550
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61600589833453	33.0	33.0	33.0	33.0	33.0
2	32.61812828877599	33.0	33.0	33.0	33.0	33.0
3	32.62192362464776	33.0	33.0	33.0	33.0	33.0
4	36.53716294149135	37.0	37.0	37.0	37.0	37.0
5	36.56245669673066	37.0	37.0	37.0	37.0	37.0
6	36.57113775237095	37.0	37.0	37.0	37.0	37.0
7	36.5440839906677	37.0	37.0	37.0	37.0	37.0
8	36.534933694235995	37.0	37.0	37.0	37.0	37.0
9	36.56925351728596	37.0	37.0	37.0	37.0	37.0
10-11	36.51982405995294	37.0	37.0	37.0	37.0	37.0
12-13	36.50566623910475	37.0	37.0	37.0	37.0	37.0
14-15	38.59710910908889	40.0	37.0	40.0	37.0	40.0
16-17	38.53856430093626	40.0	37.0	40.0	37.0	40.0
18-19	38.5501714960964	40.0	37.0	40.0	37.0	40.0
20-21	38.54625776681647	40.0	37.0	40.0	37.0	40.0
22-23	38.54177405329532	40.0	37.0	40.0	37.0	40.0
24-25	38.54193709132177	40.0	37.0	40.0	37.0	40.0
26-27	38.42112059009375	40.0	37.0	40.0	37.0	40.0
28-29	38.37125754870921	40.0	37.0	40.0	37.0	40.0
30-31	38.216872016651465	40.0	37.0	40.0	37.0	40.0
32-33	38.2369718847397	40.0	37.0	40.0	37.0	40.0
34-35	38.21222299812443	40.0	37.0	40.0	37.0	40.0
36-37	38.16339989585812	40.0	37.0	40.0	37.0	40.0
38-39	38.11523190000814	40.0	37.0	40.0	37.0	40.0
40-41	38.083848912807	40.0	37.0	40.0	33.0	40.0
42-43	38.032626879885285	40.0	37.0	40.0	33.0	40.0
44-45	37.957883175063174	40.0	37.0	40.0	33.0	40.0
46-47	37.80687930358634	40.0	37.0	40.0	33.0	40.0
48-49	37.81562935693054	40.0	37.0	40.0	33.0	40.0
50-51	37.77962852303277	40.0	37.0	40.0	33.0	40.0
52-53	37.71270823632587	40.0	37.0	40.0	33.0	40.0
54-55	37.543074316790225	40.0	37.0	40.0	33.0	40.0
56-57	37.42568614384687	40.0	37.0	40.0	33.0	40.0
58-59	37.29434815065079	38.5	37.0	40.0	33.0	40.0
60-61	37.124038667089934	37.0	37.0	40.0	33.0	40.0
62-63	36.951282331562	37.0	37.0	40.0	33.0	40.0
64-65	36.76841833075208	37.0	37.0	40.0	33.0	40.0
66-67	36.55845019213849	37.0	37.0	40.0	33.0	40.0
68-69	36.34152451257306	37.0	37.0	40.0	33.0	40.0
70-71	36.10476762763295	37.0	37.0	38.5	33.0	40.0
72-73	35.88101881938266	37.0	37.0	37.0	33.0	40.0
74-75	35.68642577821902	37.0	35.0	37.0	33.0	40.0
76-77	34.00789517867887	35.0	33.0	37.0	30.0	37.0
78-79	35.19677675865395	37.0	33.0	37.0	33.0	37.0
80-81	35.26109014963211	37.0	33.0	37.0	33.0	37.0
82-83	35.121634898245176	37.0	33.0	37.0	33.0	37.0
84-85	34.99122479634155	37.0	33.0	37.0	33.0	37.0
86-87	34.90562141164139	37.0	33.0	37.0	33.0	37.0
88-89	34.77142496114837	37.0	33.0	37.0	33.0	37.0
90-91	34.624745791097936	37.0	33.0	37.0	33.0	37.0
92-93	34.625356536148644	37.0	33.0	37.0	33.0	37.0
94-95	34.68648044175608	37.0	33.0	37.0	33.0	37.0
96-97	34.69218220790407	37.0	33.0	37.0	33.0	37.0
98-99	34.698079704669254	37.0	33.0	37.0	33.0	37.0
100	34.685685542466295	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	43.0
21	168.0
22	708.0
23	1982.0
24	4580.0
25	8999.0
26	15507.0
27	24234.0
28	35851.0
29	49969.0
30	68089.0
31	90363.0
32	119440.0
33	161079.0
34	222936.0
35	325599.0
36	578083.0
37	1446989.0
38	1750326.0
39	45599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99222308632374	19.813919665491714	14.099241498419367	24.09461574976518
2	14.86436860550848	20.593166415852785	41.706335659674174	22.83612931896456
3	18.601690721232995	25.661411358333925	29.787942753835434	25.94895516659765
4	12.723677167183444	17.685853087030733	38.12313783317005	31.46733191261577
5	13.777115674016018	37.448101726070846	35.047863368716605	13.726919231196533
6	30.714807735892734	37.12171252636584	18.498428654363348	13.665051083378078
7	27.167991435295068	32.13937845289917	22.619385724818457	18.073244386987305
8	24.574400824150853	36.88424518487845	20.6681277837816	17.8732262071891
9	25.767243188583734	16.781633713755788	20.777978361591813	36.67314473606867
10-11	24.748303912394935	26.47765269232696	28.34843974485154	20.425603650426567
12-13	25.516720364403955	23.940562159760027	28.522659098484006	22.02005837735201
14-15	22.523230290059953	25.2613521263104	26.102577704658664	26.112839878970988
16-17	22.24127622183394	28.387987193342152	26.768813566169413	22.601923018654492
18-19	22.32149963135409	27.518093949157162	28.41854945410106	21.741856965387683
20-21	23.019819055482753	26.708794469147772	28.379099721487854	21.89228675388162
22-23	22.77439621822002	26.64204401975738	28.24998737386491	22.333572388157698
24-25	22.557985641746832	26.827898979000096	28.250085369574485	22.364030009678586
26-27	22.50335093718611	26.927174177889768	28.235502541537926	22.333972343386197
28-29	22.43073143359772	26.96692397518919	28.24502444178205	22.35732014943104
30-31	22.699003672544052	26.879076212751556	28.097911572586497	22.324008542117895
32-33	22.471504589884102	26.97125622644259	28.13931947504857	22.417919708624733
34-35	22.616658211057093	26.981052832578424	28.09049990681055	22.311789049553933
36-37	22.6034738742146	26.99859436024236	27.994717745172874	22.40321402037016
38-39	22.428377688011384	27.076899680869005	28.09058882861405	22.404133802505562
40-41	22.654763261616374	26.956515567157034	27.97763770598447	22.411083465242122
42-43	22.60598011139843	27.056734620957652	27.749098119190712	22.588187148453205
44-45	22.629104646060032	27.17306445761315	27.61565152013396	22.582179376192858
46-47	22.78678436141945	27.16527864095173	27.313798809927682	22.734138187701145
48-49	22.67990144279629	27.30542840186512	27.23244265969017	22.782227495648417
50-51	22.68365089168886	27.405905284501607	27.173019804854796	22.737424018954737
52-53	22.853070044941084	27.314165689628272	27.100605736997885	22.732158528432755
54-55	22.808610592348074	27.21655872187147	27.111608346694922	22.863222339085535
56-57	22.78741216232883	27.380164950689633	27.080609247639998	22.751813639341538
58-59	22.736779237963756	27.408239582300258	27.11526828810143	22.739712891634557
60-61	22.832467097332604	27.398607596304835	27.097048062698093	22.671877243664472
62-63	22.8040146805283	27.480427776924763	27.03562119046312	22.679936352083814
64-65	22.805578820351542	27.427588345816485	26.970711652045754	22.796121181786223
66-67	22.833384081833284	27.37457571226808	26.929831577781204	22.862208628117436
68-69	22.780303108737847	27.3901581532364	26.99598959517882	22.833549142846934
70-71	22.79874123624059	27.21244690795267	27.04411984418759	22.944692011619146
72-73	22.828799423100644	27.25679357542216	26.946508830878557	22.967898170598637
74-75	22.691340527607593	27.390407639504467	26.979158475711102	22.93909335717684
76-77	22.918875901376587	27.276274063426655	26.923071040281734	22.88177899491503
78-79	22.791548098592777	27.320920719245002	26.87854613832531	23.00898504383691
80-81	22.811695302236306	27.371973761356966	26.882221539082302	22.934109397324427
82-83	22.80428760234368	27.32566283432027	26.95549218712766	22.91455737620839
84-85	22.890393538559756	27.314292329323226	26.895371354284837	22.899942777832187
86-87	22.808343372647336	27.32778528705863	26.99622903147701	22.867642308817025
88-89	22.821559292339224	27.320017210919218	26.93219849561418	22.926225001127378
90-91	22.913254584395936	27.35629125288574	26.898680071448826	22.83177409126949
92-93	22.84657617871358	27.322001812003027	26.975181474423753	22.85624053485964
94-95	22.742510336048802	27.33886845127424	27.04852144003645	22.870099772640508
96-97	22.85926179849225	27.446920152761468	27.057045091940086	22.6367729568062
98-99	24.00904027181848	29.355329483797853	27.19180175286815	19.44382849151551
100	22.84140917186973	30.37554956633582	20.977087799485464	25.805953462308988
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	983.0
1	891.0
2	1182.5
3	1677.5
4	2275.0
5	3772.0
6	5050.0
7	5875.5
8	6340.0
9	6136.0
10	5663.0
11	5131.0
12	5428.0
13	7190.5
14	11390.0
15	17258.5
16	22075.5
17	25001.5
18	26005.0
19	24819.0
20	22148.0
21	19110.5
22	17020.5
23	16940.0
24	19124.0
25	23590.0
26	29697.0
27	36851.5
28	45209.5
29	55347.5
30	66161.0
31	76798.0
32	88527.5
33	99755.5
34	110867.0
35	122000.5
36	133453.5
37	145081.5
38	155390.0
39	164093.0
40	171731.5
41	178634.0
42	183622.5
43	188765.5
44	193856.0
45	198702.0
46	202799.0
47	205913.0
48	207935.0
49	207702.5
50	206027.5
51	201856.0
52	195962.0
53	188453.0
54	179473.5
55	168935.0
56	156909.5
57	143709.0
58	128755.0
59	112126.5
60	93708.0
61	76705.0
62	61905.0
63	48272.5
64	36395.5
65	27497.5
66	21029.5
67	15966.0
68	12092.0
69	8691.5
70	6103.5
71	4452.0
72	3141.5
73	2096.5
74	1423.5
75	946.5
76	619.5
77	402.0
78	251.0
79	159.5
80	111.5
81	75.0
82	53.0
83	41.5
84	32.0
85	19.0
86	13.0
87	13.5
88	11.5
89	9.5
90	8.0
91	5.5
92	3.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	3.23196412519821E-4
7	0.0
8	0.0
9	2.0199775782488814E-5
10-11	0.0067467251113512644
12-13	0.0
14-15	0.006706325559786287
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	81.0
20-21	288.0
22-23	616.0
24-25	1613.0
26-27	1857.0
28-29	3016.0
30-31	7542.0
32-33	9809.0
34-35	13684.0
36-37	20072.0
38-39	24924.0
40-41	26337.0
42-43	31341.0
44-45	38050.0
46-47	37051.0
48-49	33754.0
50-51	30076.0
52-53	29175.0
54-55	31590.0
56-57	33597.0
58-59	33911.0
60-61	33708.0
62-63	32791.0
64-65	34203.0
66-67	37459.0
68-69	37391.0
70-71	36809.0
72-73	35225.0
74-75	36068.0
76-77	36002.0
78-79	38927.0
80-81	40162.0
82-83	39219.0
84-85	38578.0
86-87	40872.0
88-89	44638.0
90-91	46696.0
92-93	48573.0
94-95	58640.0
96-97	168545.0
98-99	309793.0
100-101	3347867.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.53265815045746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.65802777789588	81.1472884000559
2	6.258968138835621	11.082461732202779
3	1.2612789323371023	3.34993129646924
4	0.4120235652977121	1.4591016582574012
5	0.1634131255266893	0.7233699189776089
6	0.07710914191906638	0.40960063810774894
7	0.04871444415930098	0.30189734622214603
8	0.03238932489108457	0.2294010422645195
9	0.01872277556800365	0.1491819380090824
>10	0.067149387321572	0.9173842035411417
>50	0.0015080860151437132	0.08629311311399326
>100	6.626040530375485E-4	0.12828376157287516
>500	3.269617978779722E-5	0.015804951205565056
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.039955156497763E-5	0.0	0.0	0.0
3	0.0	4.039955156497763E-5	0.0	0.0	0.0
4	0.0	6.059932734746644E-5	0.0	0.0	0.0
5	0.0	8.079910312995526E-5	0.0	0.0	0.0
6	2.0199775782488814E-5	8.079910312995526E-5	0.0	0.0	0.0
7	2.0199775782488814E-5	8.079910312995526E-5	0.0	2.0199775782488814E-5	0.0
8	2.0199775782488814E-5	1.0099887891244407E-4	0.0	2.0199775782488814E-5	0.0
9	2.0199775782488814E-5	1.2119865469493288E-4	0.0	2.0199775782488814E-5	2.0199775782488814E-5
10-11	4.039955156497763E-5	1.2119865469493288E-4	0.0	3.029966367373322E-5	2.0199775782488814E-5
12-13	4.039955156497763E-5	1.2119865469493288E-4	0.0	4.039955156497763E-5	3.029966367373322E-5
14-15	4.039955156497763E-5	1.514983183686661E-4	0.0	4.039955156497763E-5	6.059932734746644E-5
16-17	4.039955156497763E-5	1.615982062599105E-4	0.0	4.039955156497763E-5	8.079910312995526E-5
18-19	4.039955156497763E-5	1.9189786993364374E-4	0.0	4.039955156497763E-5	9.089899102119966E-5
20-21	4.039955156497763E-5	2.4239730938986577E-4	0.0	6.059932734746644E-5	1.0099887891244407E-4
22-23	4.039955156497763E-5	2.6259708517235457E-4	0.0	6.059932734746644E-5	1.0099887891244407E-4
24-25	4.039955156497763E-5	3.635959640847986E-4	0.0	6.059932734746644E-5	1.0099887891244407E-4
26-27	4.039955156497763E-5	3.837957398672875E-4	0.0	1.514983183686661E-4	1.0099887891244407E-4
28-29	4.039955156497763E-5	3.837957398672875E-4	0.0	3.635959640847986E-4	1.0099887891244407E-4
30-31	6.059932734746644E-5	4.039955156497763E-4	0.0	0.0013230853137530174	1.1109876680368848E-4
32-33	6.059932734746644E-5	4.039955156497763E-4	0.0	0.003524860874044298	1.2119865469493288E-4
34-35	6.059932734746644E-5	4.4439506721475393E-4	0.0	0.006312429932027755	1.413984304774217E-4
36-37	6.059932734746644E-5	4.94894506670976E-4	0.0	0.010261486097504317	1.514983183686661E-4
38-39	6.059932734746644E-5	5.049943945622204E-4	0.0	0.01610932118653483	2.0199775782488814E-4
40-41	6.059932734746644E-5	6.46392825039642E-4	0.0	0.022522749997475028	2.1209764571613254E-4
42-43	6.059932734746644E-5	6.564927129308865E-4	0.0	0.02814838755289816	2.3229742149862137E-4
44-45	6.059932734746644E-5	7.170920402783528E-4	0.0	0.03464261546696831	2.4239730938986577E-4
46-47	6.059932734746644E-5	7.574915918433305E-4	0.0	0.04130854147518963	2.6259708517235457E-4
48-49	6.059932734746644E-5	7.67591479734575E-4	0.0	0.04760077163143489	3.029966367373322E-4
50-51	6.059932734746644E-5	7.978911434083082E-4	0.0	0.054074799769722556	3.029966367373322E-4
52-53	6.059932734746644E-5	8.18090919190797E-4	0.0	0.06099322297522497	3.130965246285766E-4
54-55	6.059932734746644E-5	8.483905828645302E-4	0.0	0.06664916019432185	3.23196412519821E-4
56-57	6.059932734746644E-5	8.483905828645302E-4	0.0	0.07113351041803437	3.23196412519821E-4
58-59	6.059932734746644E-5	8.786902465382634E-4	0.0	0.07584005817535425	3.23196412519821E-4
60-61	7.069921523871086E-5	8.988900223207523E-4	0.0	0.0810112007756714	3.23196412519821E-4
62-63	8.079910312995526E-5	9.19089798103241E-4	0.0	0.08600054539394614	3.433961883023098E-4
64-65	8.079910312995526E-5	0.0010301885649069296	0.0	0.09078789225439599	3.433961883023098E-4
66-67	8.079910312995526E-5	0.0010503883406894183	0.0	0.09583783620001818	3.433961883023098E-4
68-69	8.079910312995526E-5	0.0010604882285806627	0.0	0.10053428406944684	3.5349607619355425E-4
70-71	1.0099887891244407E-4	0.001090787892254396	0.0	0.10467523810485704	3.635959640847987E-4
72-73	1.0099887891244407E-4	0.0011210875559281293	0.0	0.10810919998788013	4.039955156497763E-4
74-75	1.0099887891244407E-4	0.0011412873317106181	0.0	0.11055337285756128	4.039955156497763E-4
76-77	1.0099887891244407E-4	0.0012018866590580845	0.0	0.11282584763309128	4.039955156497763E-4
78-79	1.0099887891244407E-4	0.0012321863227318177	0.0	0.11454282857460282	4.039955156497763E-4
80-81	1.0099887891244407E-4	0.0012321863227318177	0.0	0.11523972083909868	4.039955156497763E-4
82-83	1.0099887891244407E-4	0.0012321863227318177	0.0	0.11568411590631344	4.039955156497763E-4
84-85	1.0099887891244407E-4	0.0012321863227318177	0.0	0.11582551433679086	4.039955156497763E-4
86-87	1.0099887891244407E-4	0.0012523860985143064	0.0	0.11589621355202957	4.039955156497763E-4
88	1.0099887891244407E-4	0.001312985425861773	0.0	0.11590631343992082	4.039955156497763E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6500	0.0	47.99657	1
GTATCAA	11075	0.0	36.74119	1
TCAACGC	13395	0.0	29.54308	4
ATCAACG	13610	0.0	29.168558	3
CAACGCA	13940	0.0	28.388916	5
TATCAAC	14280	0.0	28.253267	2
AACGCAG	14290	0.0	27.663397	6
ACGCAGA	16160	0.0	24.435299	7
CGCAGAG	16710	0.0	23.657568	8
GTGGTAT	3205	0.0	22.214737	1
TACATGG	14945	0.0	21.683495	2
GTACATG	15245	0.0	21.653059	1
TGGTATC	3185	0.0	20.728472	2
ACATGGG	15515	0.0	20.386255	3
GCAGAGT	19410	0.0	20.25882	9
GAGTACT	11095	0.0	18.729803	12-13
CAGAGTA	18925	0.0	18.416452	10-11
AGAGTAC	17545	0.0	18.253656	10-11
CATGGGG	11635	0.0	18.100168	4
GTACTTT	12115	0.0	17.135849	14-15
>>END_MODULE