FastQCFastQC Report
Fri 27 May 2016
SRR2139108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7339871
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91200.049.5477831
GTATCAA158550.037.457041
TCAACGC194050.029.9495494
ATCAACG196600.029.6056753
CAACGCA200400.029.0448845
TATCAAC208400.028.055532
AACGCAG206500.028.01716
ACGCAGA234350.024.6875657
CGCAGAG238500.024.2765338
GTACATG228550.022.8013741
TACATGG232550.021.9191532
ACATGGG236500.020.9414963
GCAGAGT284450.020.3110319
GTGGTAT46550.019.8665831
GAGTACT154800.019.59515812-13
TGGTATC45250.019.2750242
AGAGTAC252450.018.73564110-11
CATGGGG184250.018.6971514
CAGAGTA274600.018.55730610-11
GTACTTT172150.017.51866514-15
Produced by FastQC (version 0.11.5)