FastQCFastQC Report
Fri 27 May 2016
SRR2139107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10689910
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133000.048.3585241
GTATCAA231350.035.7437131
TCAACGC267950.030.1950744
ATCAACG271950.029.7989853
CAACGCA277000.029.1939325
TATCAAC284400.028.7854082
AACGCAG285750.028.4066566
ACGCAGA329250.024.5877117
CGCAGAG333600.024.254278
GTACATG308800.022.4355681
TACATGG312500.021.682222
ACATGGG319200.020.5313643
GCAGAGT400100.020.0854579
GTGGTAT73950.020.0798451
GAGTACT223700.019.86939412-13
TGGTATC67250.019.6197972
CATGGGG240450.018.58094
CAGAGTA387000.018.51436410-11
GTACTTT247700.017.89159614-15
AGAGTAC357750.017.32523510-11
Produced by FastQC (version 0.11.5)