##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10689910
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.577135261194904	33.0	33.0	33.0	33.0	33.0
2	32.5812473631677	33.0	33.0	33.0	33.0	33.0
3	32.5763256192054	33.0	33.0	33.0	33.0	33.0
4	36.47496180978138	37.0	37.0	37.0	37.0	37.0
5	36.504716035962886	37.0	37.0	37.0	37.0	37.0
6	36.50125389268946	37.0	37.0	37.0	37.0	37.0
7	36.47944014495913	37.0	37.0	37.0	37.0	37.0
8	36.46078273811473	37.0	37.0	37.0	37.0	37.0
9	36.497148245401505	37.0	37.0	37.0	37.0	37.0
10-11	36.453588757997025	37.0	37.0	37.0	37.0	37.0
12-13	36.384459644655564	37.0	37.0	37.0	37.0	37.0
14-15	38.43090049401725	40.0	37.0	40.0	37.0	40.0
16-17	38.39117710064912	40.0	37.0	40.0	37.0	40.0
18-19	38.39556923304312	40.0	37.0	40.0	37.0	40.0
20-21	38.391364342583934	40.0	37.0	40.0	37.0	40.0
22-23	38.38738325338078	40.0	37.0	40.0	37.0	40.0
24-25	38.38780207672101	40.0	37.0	40.0	37.0	40.0
26-27	38.24228442763514	40.0	37.0	40.0	37.0	40.0
28-29	38.165379590533526	40.0	37.0	40.0	37.0	40.0
30-31	38.132706566945124	40.0	37.0	40.0	37.0	40.0
32-33	38.05945313072864	40.0	37.0	40.0	33.0	40.0
34-35	38.024036676421616	40.0	37.0	40.0	33.0	40.0
36-37	37.98620337172923	40.0	37.0	40.0	33.0	40.0
38-39	37.94005146530395	40.0	37.0	40.0	33.0	40.0
40-41	37.907896082847245	40.0	37.0	40.0	33.0	40.0
42-43	37.80967426267703	40.0	37.0	40.0	33.0	40.0
44-45	37.76365507325506	40.0	37.0	40.0	33.0	40.0
46-47	37.7267806432872	40.0	37.0	40.0	33.0	40.0
48-49	37.70052230315288	40.0	37.0	40.0	33.0	40.0
50-51	37.635101232811095	40.0	37.0	40.0	33.0	40.0
52-53	37.52939846860154	40.0	37.0	40.0	33.0	40.0
54-55	37.32185321106175	40.0	37.0	40.0	33.0	40.0
56-57	37.16578935699759	38.5	37.0	40.0	33.0	40.0
58-59	37.0747715558675	37.0	37.0	40.0	33.0	40.0
60-61	36.92996595903918	37.0	37.0	40.0	33.0	40.0
62-63	36.74404380846208	37.0	37.0	40.0	33.0	40.0
64-65	36.594099798574305	37.0	37.0	40.0	33.0	40.0
66-67	36.37328238531081	37.0	37.0	40.0	33.0	40.0
68-69	36.152314248545935	37.0	37.0	40.0	33.0	40.0
70-71	35.8883466792372	37.0	35.0	37.0	33.0	40.0
72-73	35.676081914634665	37.0	33.0	37.0	33.0	40.0
74-75	35.49787902651386	37.0	33.0	37.0	33.0	40.0
76-77	33.70699022531777	35.0	33.0	37.0	27.0	37.0
78-79	34.942719273595515	37.0	33.0	37.0	33.0	37.0
80-81	35.01637260099627	37.0	33.0	37.0	33.0	37.0
82-83	34.90661573836826	37.0	33.0	37.0	33.0	37.0
84-85	34.759099617723436	37.0	33.0	37.0	33.0	37.0
86-87	34.638990381678695	37.0	33.0	37.0	33.0	37.0
88-89	34.5671233531892	37.0	33.0	37.0	33.0	37.0
90-91	34.48757281288303	37.0	33.0	37.0	33.0	37.0
92-93	34.420727605340105	37.0	33.0	37.0	33.0	37.0
94-95	34.41431494758517	37.0	33.0	37.0	33.0	37.0
96-97	34.42686656167187	37.0	33.0	37.0	33.0	37.0
98-99	34.437028547846225	37.0	33.0	37.0	33.0	37.0
100	34.44480302124898	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	14.0
20	89.0
21	473.0
22	1765.0
23	5120.0
24	11813.0
25	22888.0
26	39987.0
27	62959.0
28	92520.0
29	127876.0
30	172327.0
31	226258.0
32	296714.0
33	394105.0
34	537914.0
35	769740.0
36	1315531.0
37	3040695.0
38	3491299.0
39	79823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7185364516633	19.962450572549255	14.264993811921709	24.05401916386574
2	14.72738311173808	20.5926242596991	41.84252252825328	22.837470100309545
3	18.560913983373105	25.581927256637332	29.90673448139414	25.950424278595424
4	12.663193609674916	17.816164963035234	38.381090205623806	31.13955122166604
5	13.758366534423583	37.45111979427329	35.22826665519167	13.562247016111453
6	30.63808406957291	37.3193763688144	18.553480535099087	13.489059026513605
7	27.109816640177513	32.210149570950556	22.683549253454892	17.996484535417043
8	24.438503224068302	37.08556012164742	20.68806004914915	17.78787660513512
9	25.580168588884284	16.987935352121767	20.91591042394183	36.51598563505212
10-11	24.58265359282325	26.666623634754107	28.433701059735682	20.31702171268696
12-13	25.383188190667543	24.040128777817944	28.63103899766176	21.94564403385275
14-15	22.355914957886085	25.488160654064174	26.200905146025338	25.955019242024402
16-17	22.121140402491697	28.58332764260878	26.90407122230215	22.391460732597373
18-19	22.151790800858006	27.765799712064926	28.51015583854307	21.572253648533994
20-21	22.822417459323987	26.91500313364638	28.510985443032677	21.751593963996953
22-23	22.602594874645206	26.880623613414468	28.335852246858543	22.18092926508178
24-25	22.392781926857523	27.036544288438463	28.348101183502216	22.222572601201797
26-27	22.351226784338596	27.176842004075123	28.27549773360603	22.19643347798025
28-29	22.279674608560946	27.25017060615186	28.297385250040076	22.17276953524712
30-31	22.548499243712815	27.086681498031922	28.24687312670463	22.117946131550635
32-33	22.313997595065832	27.209580179859653	28.21384098160946	22.262581243465057
34-35	22.468876281978474	27.23929605337265	28.178979300793145	22.11284836385573
36-37	22.429212602012306	27.21779203837018	28.111901533570116	22.241093826047393
38-39	22.30931601723661	27.279986274855844	28.167487605081952	22.243210102825593
40-41	22.55840672253175	27.139646230858656	28.05844419978609	22.243502846823507
42-43	22.45926784633675	27.260778254908484	27.875572802058468	22.404381096696298
44-45	22.50406524498767	27.333823787997723	27.728213227099918	22.433897739914684
46-47	22.61425082779958	27.328953007058175	27.476437136121273	22.58035902902097
48-49	22.5302383372835	27.478462870529675	27.439352387156074	22.55194640503075
50-51	22.54978247547567	27.59521350729951	27.32474432076053	22.530259696464288
52-53	22.644589000010317	27.49397080143512	27.28219596946418	22.579244229090385
54-55	22.623744484749583	27.39526412736891	27.309269847581003	22.671721540300503
56-57	22.61307957518717	27.537875270448968	27.22899858524835	22.62004656911551
58-59	22.556606388712957	27.571757652527417	27.280414098569754	22.59122186018987
60-61	22.672423170194907	27.54043091001412	27.260107117754146	22.52703880203683
62-63	22.59229905624662	27.64067931858375	27.239652088731575	22.527369536438055
64-65	22.603356958974995	27.597462523939292	27.144701424214134	22.654479092871572
66-67	22.644453944052128	27.58145150662757	27.058395258893768	22.715699290426535
68-69	22.591228519294738	27.583582726157264	27.102494811228894	22.722693943319104
70-71	22.601970626626848	27.412890091236306	27.14402557159526	22.841113710541585
72-73	22.656423806858303	27.438021985693613	27.0765300050172	22.829024202430883
74-75	22.570382767727278	27.53870498547298	27.059235990470597	22.831676256329146
76-77	22.741312722750443	27.470370141463608	26.985691034309905	22.802626101476044
78-79	22.65593207183207	27.45776829902123	26.985277637415262	22.90102199173144
80-81	22.663796208954086	27.549974214695183	26.99688526998902	22.789344306361713
82-83	22.679314122538162	27.51289717297919	27.062786316142684	22.745002388339962
84-85	22.768114553290577	27.513134113911885	27.00756558194718	22.711185750850362
86-87	22.670680455691485	27.5291943366404	27.086756252302063	22.71336895536605
88-89	22.674466575822326	27.52124699684697	27.05493317334602	22.74935325398469
90-91	22.72314745305736	27.577646256353667	27.009580718104537	22.689625572484434
92-93	22.747779928025068	27.477695114687155	27.100199112474677	22.6743258448131
94-95	22.595159368762456	27.554616455814035	27.15243981589208	22.69778435953143
96-97	22.681221650297825	27.652723788198415	27.183243057245406	22.48281150425836
98-99	23.814042103208976	29.52995893203277	27.358517286445338	19.29748167831292
100	22.66983735517089	30.5558358000522	21.18182757624908	25.592499268527824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1636.0
1	1490.0
2	2101.5
3	3031.5
4	4151.0
5	7016.5
6	9466.0
7	10877.5
8	11605.5
9	11403.5
10	10662.0
11	9689.5
12	10531.5
13	14101.0
14	22483.5
15	34577.0
16	45137.0
17	51794.5
18	53634.0
19	51009.0
20	45780.0
21	39855.5
22	35908.5
23	36207.0
24	40889.5
25	50364.0
26	63569.0
27	79298.5
28	98016.0
29	120145.0
30	144141.5
31	168658.5
32	194265.0
33	219696.5
34	245450.5
35	269970.0
36	294578.0
37	319030.0
38	341313.5
39	360297.5
40	378975.0
41	396776.5
42	407004.5
43	416396.5
44	426635.0
45	436180.0
46	444490.5
47	448596.0
48	449000.5
49	444092.0
50	436724.5
51	427292.5
52	412477.5
53	392774.0
54	371246.5
55	346247.5
56	318562.5
57	289302.0
58	256362.0
59	221104.5
60	183173.5
61	148088.5
62	118067.0
63	91169.0
64	67914.0
65	50153.0
66	37845.0
67	28977.5
68	22004.5
69	15750.0
70	11043.5
71	8002.5
72	5627.0
73	3765.0
74	2551.0
75	1697.0
76	1111.0
77	739.0
78	523.0
79	382.0
80	271.0
81	191.5
82	133.5
83	97.0
84	67.5
85	46.5
86	37.5
87	24.0
88	14.5
89	13.0
90	11.5
91	9.5
92	7.0
93	4.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.870923141541884E-5
7	0.0
8	0.0
9	0.0
10-11	0.0015809300546028918
12-13	2.385427005465902E-4
14-15	0.007539820260413792
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	164.0
20-21	646.0
22-23	1348.0
24-25	3241.0
26-27	3703.0
28-29	5664.0
30-31	13265.0
32-33	16830.0
34-35	23946.0
36-37	35055.0
38-39	41500.0
40-41	47044.0
42-43	56452.0
44-45	68912.0
46-47	67629.0
48-49	61552.0
50-51	53088.0
52-53	50871.0
54-55	54957.0
56-57	61312.0
58-59	63426.0
60-61	65638.0
62-63	64993.0
64-65	70019.0
66-67	75528.0
68-69	76397.0
70-71	77091.0
72-73	73891.0
74-75	77316.0
76-77	75560.0
78-79	83160.0
80-81	86436.0
82-83	84944.0
84-85	85773.0
86-87	90751.0
88-89	99273.0
90-91	104988.0
92-93	112144.0
94-95	136851.0
96-97	387969.0
98-99	685806.0
100-101	7344777.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.40166656868873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.43142805048764	73.75328476459757
2	8.054913115866338	13.435863558584838
3	1.9800645182894865	4.95422042216613
4	0.6971083512133441	2.325599930805747
5	0.3207352116096479	1.3374925587752835
6	0.16697222161487363	0.8355456932014135
7	0.10339505282272327	0.6036323804260906
8	0.06364082419216606	0.42462006395452573
9	0.03980762439736237	0.29880199951923736
>10	0.13838364889468208	1.7836523759668612
>50	0.002864925603158166	0.1630030257153978
>100	6.864550085668036E-4	0.08428322628691987
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.35461570770942E-6
2	0.0	2.806384712312826E-5	0.0	0.0	9.35461570770942E-6
3	0.0	2.806384712312826E-5	0.0	0.0	9.35461570770942E-6
4	0.0	8.419154136938478E-5	0.0	9.35461570770942E-6	9.35461570770942E-6
5	0.0	9.35461570770942E-5	0.0	9.35461570770942E-6	9.35461570770942E-6
6	6.548230995396593E-5	9.35461570770942E-5	0.0	1.870923141541884E-5	9.35461570770942E-6
7	6.548230995396593E-5	9.35461570770942E-5	0.0	2.806384712312826E-5	9.35461570770942E-6
8	6.548230995396593E-5	1.0290077278480362E-4	0.0	2.806384712312826E-5	1.870923141541884E-5
9	6.548230995396593E-5	1.5902846703106014E-4	0.0	3.741846283083768E-5	1.870923141541884E-5
10-11	6.548230995396593E-5	1.5902846703106014E-4	0.0	3.741846283083768E-5	1.870923141541884E-5
12-13	6.548230995396593E-5	1.6370577488491485E-4	0.0	4.209577068469239E-5	1.870923141541884E-5
14-15	8.419154136938478E-5	2.432200084004449E-4	0.0	6.548230995396593E-5	2.806384712312826E-5
16-17	1.1225538849251304E-4	2.432200084004449E-4	0.0	6.548230995396593E-5	2.806384712312826E-5
18-19	1.1225538849251304E-4	2.525746241081543E-4	0.0	6.548230995396593E-5	2.806384712312826E-5
20-21	1.3096461990793186E-4	2.7128385552357314E-4	0.0	7.015961780782064E-5	2.806384712312826E-5
22-23	1.3096461990793186E-4	3.133796262082656E-4	0.0	8.419154136938478E-5	3.741846283083768E-5
24-25	1.3096461990793186E-4	3.3208885762368444E-4	0.0	1.0757808063865833E-4	5.145038639240181E-5
26-27	1.4031923561564128E-4	3.367661654775391E-4	0.0	2.0112423771575253E-4	5.612769424625652E-5
28-29	1.4967385132335072E-4	3.367661654775391E-4	0.0	5.6595425031642E-4	5.612769424625652E-5
30-31	1.4967385132335072E-4	3.9289385972379563E-4	0.0	0.00209543391852691	5.612769424625652E-5
32-33	1.4967385132335072E-4	3.9289385972379563E-4	0.0	0.006202110214211345	5.612769424625652E-5
34-35	1.6838308273876956E-4	4.490215539700521E-4	0.0	0.011880361948790961	5.612769424625652E-5
36-37	1.824150063003337E-4	5.098265560701633E-4	0.0	0.02080466533394575	5.612769424625652E-5
38-39	1.9644692986189782E-4	5.332130953394369E-4	0.0	0.0418151322134611	6.548230995396594E-5
40-41	2.0112423771575253E-4	6.080500210011123E-4	0.0	0.06468716761881063	7.483692566167536E-5
42-43	2.338653926927355E-4	6.220819445626764E-4	0.0	0.08044501777844715	7.483692566167536E-5
44-45	2.338653926927355E-4	7.390146409090442E-4	0.0	0.09929924573733549	7.951423351553007E-5
46-47	2.338653926927355E-4	8.232061822784289E-4	0.0	0.11884103795074047	8.886884922323949E-5
48-49	2.338653926927355E-4	8.278834901322837E-4	0.0	0.13674109510744242	9.35461570770942E-5
50-51	2.385427005465902E-4	9.02720415793959E-4	0.0	0.15325199183154958	9.35461570770942E-5
52-53	2.432200084004449E-4	9.073977236478137E-4	0.0	0.19132995506978073	1.0290077278480362E-4
54-55	2.525746241081543E-4	9.120750315016684E-4	0.0	0.23636775239454777	1.1225538849251304E-4
56-57	2.619292398158637E-4	9.261069550632325E-4	0.0	0.27902012271384885	1.2628731205407715E-4
58-59	2.7128385552357314E-4	9.307842629170872E-4	0.0	0.30773411562866293	1.4031923561564128E-4
60-61	2.8063847123128256E-4	9.494934943325061E-4	0.0	0.3276360605468147	1.4031923561564128E-4
62-63	2.8063847123128256E-4	9.728800336017795E-4	0.0	0.3405781713784307	1.4031923561564128E-4
64-65	2.8063847123128256E-4	0.001113199269217421	0.0	0.34482048960187694	1.4031923561564128E-4
66-67	2.8063847123128256E-4	0.0011319085006328397	0.0	0.34872136435199175	1.4031923561564128E-4
68-69	2.8999308693899203E-4	0.0011646496556098227	0.0	0.35273917179845293	1.4499654346949601E-4
70-71	2.9467039479284677E-4	0.0012207773498560791	0.0	0.3564202130794366	1.5435115917720543E-4
72-73	3.180569340621203E-4	0.0012348092734176435	0.0	0.35948384972371145	1.6838308273876956E-4
74-75	3.367661654775391E-4	0.001258195812686917	0.0	0.361976854809816	1.6838308273876956E-4
76-77	3.367661654775391E-4	0.0012909369676638999	0.0	0.36465227490222085	1.6838308273876956E-4
78-79	3.367661654775391E-4	0.0013002915833716093	0.0	0.3663782015002933	1.6838308273876956E-4
80-81	3.367661654775391E-4	0.0013002915833716093	0.0	0.3669394784427558	1.7773769844647898E-4
82-83	3.367661654775391E-4	0.0013096461990793188	0.0	0.3672294715296949	1.9644692986189782E-4
84-85	3.367661654775391E-4	0.0013517419697640111	0.0	0.3674399503831183	2.0112423771575253E-4
86-87	3.367661654775391E-4	0.0013564192776178657	0.0	0.36754752846375693	2.0580154556960724E-4
88	3.367661654775391E-4	0.0014593200504026695	0.0	0.3675522057716108	2.0580154556960724E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13300	0.0	48.358524	1
GTATCAA	23135	0.0	35.743713	1
TCAACGC	26795	0.0	30.195074	4
ATCAACG	27195	0.0	29.798985	3
CAACGCA	27700	0.0	29.193932	5
TATCAAC	28440	0.0	28.785408	2
AACGCAG	28575	0.0	28.406656	6
ACGCAGA	32925	0.0	24.587711	7
CGCAGAG	33360	0.0	24.25427	8
GTACATG	30880	0.0	22.435568	1
TACATGG	31250	0.0	21.68222	2
ACATGGG	31920	0.0	20.531364	3
GCAGAGT	40010	0.0	20.085457	9
GTGGTAT	7395	0.0	20.079845	1
GAGTACT	22370	0.0	19.869394	12-13
TGGTATC	6725	0.0	19.619797	2
CATGGGG	24045	0.0	18.5809	4
CAGAGTA	38700	0.0	18.514364	10-11
GTACTTT	24770	0.0	17.891596	14-15
AGAGTAC	35775	0.0	17.325235	10-11
>>END_MODULE