FastQCFastQC Report
Fri 27 May 2016
SRR2139106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9932699
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130000.049.400441
GTATCAA227900.037.8929561
TCAACGC271750.031.2784214
ATCAACG277600.030.6508583
CAACGCA281550.030.2061845
TATCAAC287500.029.8088682
AACGCAG288400.029.5495366
ACGCAGA333600.025.4669767
CGCAGAG340750.024.9197378
GTACATG311450.023.0970651
TACATGG314100.022.3160382
ACATGGG320600.021.39873
GTGGTAT63000.021.0833021
GCAGAGT402450.021.0806649
GAGTACT228000.020.2678612-13
TGGTATC64850.019.1299132
CAGAGTA386850.019.08599310-11
CATGGGG243850.019.0374934
AGAGTAC358550.018.53196510-11
GTACTTT249350.018.4888914-15
Produced by FastQC (version 0.11.5)