##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139106_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9932699
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55726827119195	33.0	33.0	33.0	33.0	33.0
2	32.56467864374024	33.0	33.0	33.0	33.0	33.0
3	32.558850620561444	33.0	33.0	33.0	33.0	33.0
4	36.454794613226476	37.0	37.0	37.0	37.0	37.0
5	36.48421783444761	37.0	37.0	37.0	37.0	37.0
6	36.484218337835465	37.0	37.0	37.0	37.0	37.0
7	36.459738989372376	37.0	37.0	37.0	37.0	37.0
8	36.442460100723885	37.0	37.0	37.0	37.0	37.0
9	36.48009830963367	37.0	37.0	37.0	37.0	37.0
10-11	36.43389636593236	37.0	37.0	37.0	37.0	37.0
12-13	36.366214208242894	37.0	37.0	37.0	37.0	37.0
14-15	38.38608458788492	40.0	37.0	40.0	37.0	40.0
16-17	38.345079620352934	40.0	37.0	40.0	37.0	40.0
18-19	38.350377425108725	40.0	37.0	40.0	37.0	40.0
20-21	38.34509917136032	40.0	37.0	40.0	37.0	40.0
22-23	38.34250166377332	40.0	37.0	40.0	37.0	40.0
24-25	38.34212677432447	40.0	37.0	40.0	37.0	40.0
26-27	38.189427564509174	40.0	37.0	40.0	37.0	40.0
28-29	38.107934355964524	40.0	37.0	40.0	33.0	40.0
30-31	38.07300755922931	40.0	37.0	40.0	33.0	40.0
32-33	37.99587286976491	40.0	37.0	40.0	33.0	40.0
34-35	37.95620823867951	40.0	37.0	40.0	33.0	40.0
36-37	37.91782865453609	40.0	37.0	40.0	33.0	40.0
38-39	37.8648049545464	40.0	37.0	40.0	33.0	40.0
40-41	37.8329979325342	40.0	37.0	40.0	33.0	40.0
42-43	37.734228024965475	40.0	37.0	40.0	33.0	40.0
44-45	37.67939050315376	40.0	37.0	40.0	33.0	40.0
46-47	37.639608391356376	40.0	37.0	40.0	33.0	40.0
48-49	37.6085808703584	40.0	37.0	40.0	33.0	40.0
50-51	37.53935427823659	40.0	37.0	40.0	33.0	40.0
52-53	37.4348731583806	40.0	37.0	40.0	33.0	40.0
54-55	37.2173773785263	40.0	37.0	40.0	33.0	40.0
56-57	37.02874870960328	37.0	37.0	40.0	33.0	40.0
58-59	36.96053720357068	37.0	37.0	40.0	33.0	40.0
60-61	36.818586509019966	37.0	37.0	40.0	33.0	40.0
62-63	36.626625504882256	37.0	37.0	40.0	33.0	40.0
64-65	36.481846431960804	37.0	37.0	40.0	33.0	40.0
66-67	36.25912230137622	37.0	37.0	40.0	33.0	40.0
68-69	36.03614116999378	37.0	37.0	40.0	33.0	40.0
70-71	35.774411155044305	37.0	33.0	37.0	33.0	40.0
72-73	35.567692731172954	37.0	33.0	37.0	33.0	40.0
74-75	35.39346196476244	37.0	33.0	37.0	33.0	40.0
76-77	33.5957540131954	35.0	33.0	37.0	27.0	37.0
78-79	34.84961114496091	37.0	33.0	37.0	33.0	37.0
80-81	34.92376374239626	37.0	33.0	37.0	33.0	37.0
82-83	34.81710525495353	37.0	33.0	37.0	33.0	37.0
84-85	34.673348992468185	37.0	33.0	37.0	33.0	37.0
86-87	34.55522182182369	37.0	33.0	37.0	33.0	37.0
88-89	34.48203349931849	37.0	33.0	37.0	33.0	37.0
90-91	34.40281985319933	37.0	33.0	37.0	30.0	37.0
92-93	34.3366673354086	37.0	33.0	37.0	27.0	37.0
94-95	34.32537360039569	37.0	33.0	37.0	27.0	37.0
96-97	34.3417778126894	37.0	33.0	37.0	33.0	37.0
98-99	34.35878731315508	37.0	33.0	37.0	33.0	37.0
100	34.36722273574544	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	9.0
20	111.0
21	486.0
22	1950.0
23	5400.0
24	12145.0
25	23801.0
26	40419.0
27	63202.0
28	91847.0
29	126120.0
30	167947.0
31	220234.0
32	288718.0
33	380728.0
34	516473.0
35	742762.0
36	1270430.0
37	2875082.0
38	3047573.0
39	57257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72598001811995	19.912382324280642	14.30797409646663	24.05366356113278
2	14.773547451704719	20.62014564218648	41.67487608352977	22.93143082257904
3	18.398725260878237	25.767779734390423	30.03746514416676	25.796029860564584
4	12.683531434910089	17.799452092527922	38.233052265049004	31.28396420751298
5	13.705378568302534	37.525389624713284	35.170702343844304	13.598529463139878
6	30.627801227823497	37.28939508681327	18.488169075512012	13.59463460985122
7	27.06242281176546	32.30738191099922	22.620206250083687	18.009989027151633
8	24.65593692107251	36.833442753072454	20.667333219299206	17.84328710655583
9	25.78193500074854	16.759855503524268	20.747110125858036	36.71109936986915
10-11	24.642925730394346	26.535166529281845	28.39662473807741	20.4252830022464
12-13	25.485667352584745	23.971341853295684	28.52620336942533	22.01678742469424
14-15	22.475995204981405	25.35468276497703	26.133103046786065	26.036218983255495
16-17	22.20516296728613	28.47407336112773	26.789652037175394	22.531111634410745
18-19	22.22970815888008	27.58816108290405	28.461503766498915	21.720626991716955
20-21	23.006639358827922	26.73155676327104	28.404041909159233	21.857761968741805
22-23	22.752142933701826	26.73357462581634	28.235383967030263	22.278898473451576
24-25	22.52950279924239	26.888890656882097	28.301503245179514	22.280103298695998
26-27	22.49112715147058	27.013166974769177	28.25858447465584	22.237121399104403
28-29	22.388785325148433	27.02981375961842	28.270700757944144	22.310700157289006
30-31	22.703005163288665	26.909286809849203	28.193216052453586	22.19449197440855
32-33	22.532362318632057	27.00800100160818	28.18318424062425	22.276452439135515
34-35	22.551019505283023	27.062366091574084	28.231535331599922	22.155079071542964
36-37	22.58385264505427	27.016042891303897	28.065432263415392	22.33467220022644
38-39	22.43816509412436	27.10541970876552	28.141258752773098	22.315156444337024
40-41	22.67535014289866	26.985778366657826	28.023538361876433	22.31533312856708
42-43	22.596988082100538	27.069748836843488	27.835124868814624	22.498138212241347
44-45	22.6107914544439	27.174647285912783	27.723465196769858	22.49109606287346
46-47	22.758065074885348	27.127714359882475	27.397721781953706	22.71649878327847
48-49	22.647898371619846	27.262149959726052	27.37197948619657	22.717972182457537
50-51	22.642111192781204	27.44732279160934	27.27354873296184	22.637017282647616
52-53	22.79276145886917	27.334544994462124	27.215510384292056	22.657183162376647
54-55	22.73153419858946	27.23033779537979	27.231389055954796	22.806738950075953
56-57	22.758554406236062	27.408375990314564	27.13218177184002	22.700887831609354
58-59	22.661395864689744	27.401112059140992	27.194747104924932	22.742744971244328
60-61	22.855891100075397	27.407934619138786	27.19074236368656	22.545431917099258
62-63	22.702295362848375	27.47832571350947	27.169446874613918	22.64993204902824
64-65	22.683327229565887	27.433527042258277	27.117614344166423	22.765531384009417
66-67	22.71387757254293	27.396525217730566	27.156223131172748	22.733374078553762
68-69	22.659368248446228	27.480684567132467	27.118535852953872	22.741411331467432
70-71	22.67963815802058	27.288828161874477	27.03666406431795	22.99486961578699
72-73	22.711283879612658	27.35346893509756	26.94076783260458	22.99447935268521
74-75	22.61756602124786	27.433554227046148	27.084408360843064	22.864471390862924
76-77	22.795457737233683	27.2259595259933	26.998366731696148	22.98021600507687
78-79	22.695990363340464	27.248483414705504	26.860045577559568	23.19548064439446
80-81	22.853505187754767	27.45212186717787	26.822100551759302	22.872272393308062
82-83	22.903194703199446	27.30920280248631	26.87838298986926	22.909219504444977
84-85	22.85126559643253	27.291123060496215	26.840037391361648	23.01757395170961
86-87	22.71893722903574	27.408406054390777	26.962639416080524	22.910017300492957
88-89	22.850404962978036	27.380171242103252	26.92317702342534	22.846246771493377
90-91	22.90495489369199	27.42151468526256	26.883513243912866	22.790017177132587
92-93	22.80883188238461	27.269057158064054	26.938690058198787	22.98342090135255
94-95	22.796358808631656	27.425183243707803	26.995647232713427	22.782810714947114
96-97	23.01376504790581	27.40402855676191	27.004863055274765	22.57734334005751
98-99	23.962287283115455	29.381236801407084	27.248408278036184	19.408067637441274
100	22.805352802974767	30.408591437745912	21.092286528043328	25.69376923123599
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1698.0
1	1554.5
2	2188.5
3	3118.0
4	4266.5
5	7142.5
6	9599.5
7	11178.5
8	12130.5
9	11853.5
10	10925.0
11	9888.0
12	10486.0
13	13743.0
14	21901.0
15	33825.0
16	44171.0
17	50523.5
18	52048.5
19	49186.5
20	43673.0
21	37519.5
22	32981.5
23	32198.0
24	36121.5
25	44682.0
26	56710.5
27	70813.0
28	87126.0
29	107019.5
30	128355.5
31	149551.5
32	172314.5
33	195006.0
34	217801.5
35	240400.5
36	264443.5
37	287507.5
38	307428.0
39	324996.5
40	340827.0
41	353406.5
42	361637.0
43	372047.0
44	382806.0
45	392982.0
46	400393.5
47	403495.0
48	406396.5
49	407029.0
50	405705.0
51	399752.0
52	386793.5
53	370082.5
54	350896.0
55	329357.5
56	304753.5
57	275971.5
58	245734.5
59	214159.5
60	179015.5
61	145138.0
62	115779.5
63	89650.5
64	66783.5
65	49372.5
66	36813.5
67	27949.5
68	21233.0
69	15074.5
70	10346.0
71	7235.0
72	4881.0
73	3157.0
74	1963.0
75	1196.0
76	793.0
77	512.5
78	350.0
79	260.5
80	197.5
81	145.0
82	98.5
83	64.0
84	49.0
85	37.0
86	26.5
87	19.5
88	13.5
89	11.0
90	9.0
91	7.0
92	7.5
93	5.0
94	2.5
95	3.0
96	3.0
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0067757011462847E-5
7	0.0
8	0.0
9	0.0
10-11	0.0013742488320646784
12-13	2.61761682298034E-4
14-15	0.007299123833310563
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	159.0
20-21	601.0
22-23	1292.0
24-25	3349.0
26-27	3657.0
28-29	5218.0
30-31	11091.0
32-33	13967.0
34-35	19660.0
36-37	27136.0
38-39	32730.0
40-41	37915.0
42-43	47566.0
44-45	58343.0
46-47	56967.0
48-49	50928.0
50-51	43776.0
52-53	42112.0
54-55	47069.0
56-57	51662.0
58-59	34536.0
60-61	57444.0
62-63	56580.0
64-65	61288.0
66-67	67102.0
68-69	65732.0
70-71	66511.0
72-73	65373.0
74-75	67889.0
76-77	66728.0
78-79	74368.0
80-81	77002.0
82-83	76508.0
84-85	77539.0
86-87	83808.0
88-89	90451.0
90-91	97516.0
92-93	105378.0
94-95	128293.0
96-97	386348.0
98-99	649425.0
100-101	6921682.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.34817313408985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.4913865608192	72.87104021386011
2	7.871296743561904	12.963738140572667
3	1.9368927210459286	4.7849873140454555
4	0.7448842195858547	2.4535941871722944
5	0.34131318312724357	1.4053258548554786
6	0.19336540912745134	0.9553972913382889
7	0.1099141046398501	0.6335858003132591
8	0.0714108982132048	0.4704445607777486
9	0.047219176808813906	0.3499571652391267
>10	0.18426371047359616	2.4664427458744473
>50	0.005728916024612272	0.32725915892642404
>100	0.0022754945141827744	0.2843681211680753
>500	4.886205823465331E-5	0.033859445856615586
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0067757011462845E-5	0.0	0.0	0.0
3	0.0	1.0067757011462845E-5	0.0	0.0	0.0
4	0.0	2.013551402292569E-5	0.0	1.0067757011462845E-5	0.0
5	0.0	2.013551402292569E-5	0.0	1.0067757011462845E-5	0.0
6	0.0	2.013551402292569E-5	0.0	1.0067757011462845E-5	0.0
7	0.0	2.013551402292569E-5	0.0	2.013551402292569E-5	0.0
8	0.0	5.033878505731423E-5	0.0	2.013551402292569E-5	1.0067757011462845E-5
9	0.0	1.4094859816047984E-4	0.0	2.013551402292569E-5	1.0067757011462845E-5
10-11	0.0	1.4094859816047984E-4	0.0	3.0203271034388538E-5	1.0067757011462845E-5
12-13	0.0	1.5101635517194268E-4	0.0	3.0203271034388538E-5	1.0067757011462845E-5
14-15	0.0	2.1142289724071977E-4	0.0	3.0203271034388538E-5	1.5101635517194269E-5
16-17	0.0	2.2652453275791404E-4	0.0	4.027102804585138E-5	2.013551402292569E-5
18-19	0.0	2.3659228976937688E-4	0.0	4.027102804585138E-5	3.0203271034388538E-5
20-21	0.0	2.5169392528657115E-4	0.0	5.033878505731423E-5	3.0203271034388538E-5
22-23	0.0	3.121004673553482E-4	0.0	6.54404205745085E-5	3.523714954011996E-5
24-25	0.0	3.423037383897368E-4	0.0	7.550817758597134E-5	5.5372663563045654E-5
26-27	0.0	3.7754088792985675E-4	0.0	1.2584696264328557E-4	6.0406542068777076E-5
28-29	0.0	3.92642523447051E-4	0.0	2.1142289724071977E-4	6.0406542068777076E-5
30-31	0.0	4.530490655158281E-4	0.0	9.211997665488504E-4	6.0406542068777076E-5
32-33	0.0	4.832523365502166E-4	0.0	0.0027283621501064314	6.0406542068777076E-5
34-35	0.0	5.48692757124725E-4	0.0	0.005119454440328857	7.047429908023992E-5
36-37	0.0	6.493703272393535E-4	0.0	0.009503962618820926	7.047429908023992E-5
38-39	0.0	6.745397197680106E-4	0.0	0.02136881425682989	7.047429908023992E-5
40-41	0.0	8.154883179284906E-4	0.0	0.03480423598862706	7.047429908023992E-5
42-43	0.0	8.406577104571476E-4	0.0	0.04392058996250667	7.550817758597134E-5
44-45	0.0	9.363014020660448E-4	0.0	0.0557804077220099	8.55759345974342E-5
46-47	1.0067757011462845E-5	9.967079441348219E-4	0.0	0.06736839604220363	1.0067757011462847E-4
48-49	1.0067757011462845E-5	0.001011809579652016	0.0	0.0787852324932025	1.0067757011462847E-4
50-51	1.5101635517194269E-5	0.0011879953273526158	0.0	0.09012152688810968	1.0067757011462847E-4
52-53	2.013551402292569E-5	0.0011980630843640787	0.0	0.1184823983894005	1.1577920563182273E-4
54-55	2.013551402292569E-5	0.0011980630843640787	0.0	0.15335207479860208	1.3591471965474842E-4
56-57	2.013551402292569E-5	0.0011980630843640787	0.0	0.18907751055377797	1.4598247666621126E-4
58-59	2.013551402292569E-5	0.001213164719881273	0.0	0.31631382366464544	1.5101635517194268E-4
60-61	2.013551402292569E-5	0.0012232324768927357	0.0	0.427391386772115	1.5101635517194268E-4
62-63	2.013551402292569E-5	0.0012333002339041986	0.0	0.43275246738071893	1.560502336776741E-4
64-65	2.013551402292569E-5	0.0013490794395360213	0.0	0.43686514611990157	1.6108411218340553E-4
66-67	2.013551402292569E-5	0.0013490794395360213	0.0	0.4406858599057517	1.711518691948684E-4
68-69	2.013551402292569E-5	0.0013994182245933356	0.0	0.44465255616826804	1.8121962620633124E-4
70-71	2.013551402292569E-5	0.0014094859816047983	0.0	0.4481863388792915	1.963212617235255E-4
72-73	2.013551402292569E-5	0.0014145198601105298	0.0	0.4515439358426144	2.1142289724071977E-4
74-75	2.013551402292569E-5	0.0014447231311449185	0.0	0.45439311107685837	2.1142289724071977E-4
76-77	2.013551402292569E-5	0.0014950619162022328	0.0	0.45692011808673555	2.1142289724071977E-4
78-79	2.013551402292569E-5	0.001510163551719427	0.0	0.45846551878799513	2.1142289724071977E-4
80-81	2.013551402292569E-5	0.001510163551719427	0.0	0.4590242793021313	2.1142289724071977E-4
82-83	2.013551402292569E-5	0.001510163551719427	0.0	0.4595175993956929	2.466600467808397E-4
84-85	2.013551402292569E-5	0.0015202313087308897	0.0	0.45972398841442796	2.5672780379230257E-4
86-87	2.013551402292569E-5	0.0015202313087308897	0.0	0.45980956434902537	2.667955608037654E-4
88	2.013551402292569E-5	0.0016410443928684439	0.0	0.4598246659845426	2.7182943930949684E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13000	0.0	49.40044	1
GTATCAA	22790	0.0	37.892956	1
TCAACGC	27175	0.0	31.278421	4
ATCAACG	27760	0.0	30.650858	3
CAACGCA	28155	0.0	30.206184	5
TATCAAC	28750	0.0	29.808868	2
AACGCAG	28840	0.0	29.549536	6
ACGCAGA	33360	0.0	25.466976	7
CGCAGAG	34075	0.0	24.919737	8
GTACATG	31145	0.0	23.097065	1
TACATGG	31410	0.0	22.316038	2
ACATGGG	32060	0.0	21.3987	3
GTGGTAT	6300	0.0	21.083302	1
GCAGAGT	40245	0.0	21.080664	9
GAGTACT	22800	0.0	20.26786	12-13
TGGTATC	6485	0.0	19.129913	2
CAGAGTA	38685	0.0	19.085993	10-11
CATGGGG	24385	0.0	19.037493	4
AGAGTAC	35855	0.0	18.531965	10-11
GTACTTT	24935	0.0	18.48889	14-15
>>END_MODULE