FastQCFastQC Report
Fri 27 May 2016
SRR2139105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9704705
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161150.044.0288351
GTATCAA263200.034.1796071
ATCAACG317550.027.5572513
TCAACGC318800.027.5316314
CAACGCA321800.027.2351175
AACGCAG328150.026.8282186
TATCAAC330950.026.8120232
ACGCAGA369800.023.7118457
CGCAGAG371600.023.5740228
GTACATG331950.021.770271
TACATGG331350.021.162012
ACATGGG329550.020.6263773
GCAGAGT432000.020.2203169
GAGTACT247150.020.0196512-13
ATCTCGT91250.020.00747394
CATGGGG231600.019.0055334
AGAGTAC393250.018.54221310-11
CAGAGTA423200.018.53943310-11
TATCTCG106050.018.53035294
GTACTAG27500.017.8376621
Produced by FastQC (version 0.11.5)