##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139105_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9704705
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58079333684022	33.0	33.0	33.0	33.0	33.0
2	32.59133750072774	33.0	33.0	33.0	33.0	33.0
3	32.58204788295986	33.0	33.0	33.0	33.0	33.0
4	36.48520228074939	37.0	37.0	37.0	37.0	37.0
5	36.51605597491114	37.0	37.0	37.0	37.0	37.0
6	36.51302074612263	37.0	37.0	37.0	37.0	37.0
7	36.490089085654844	37.0	37.0	37.0	37.0	37.0
8	36.47488759318289	37.0	37.0	37.0	37.0	37.0
9	36.506708859259504	37.0	37.0	37.0	37.0	37.0
10-11	36.46417139933671	37.0	37.0	37.0	37.0	37.0
12-13	36.39517651489664	37.0	37.0	37.0	37.0	37.0
14-15	38.438951828005074	40.0	37.0	40.0	37.0	40.0
16-17	38.39912800028439	40.0	37.0	40.0	37.0	40.0
18-19	38.41446071776525	40.0	37.0	40.0	37.0	40.0
20-21	38.416356310140145	40.0	37.0	40.0	37.0	40.0
22-23	38.411450760161756	40.0	37.0	40.0	37.0	40.0
24-25	38.40641461397395	40.0	37.0	40.0	37.0	40.0
26-27	38.259289896451065	40.0	37.0	40.0	37.0	40.0
28-29	38.18697800799475	40.0	37.0	40.0	37.0	40.0
30-31	38.14688210777804	40.0	37.0	40.0	37.0	40.0
32-33	38.06858865189611	40.0	37.0	40.0	33.0	40.0
34-35	38.03015834730067	40.0	37.0	40.0	33.0	40.0
36-37	37.98915265318118	40.0	37.0	40.0	33.0	40.0
38-39	37.93873674886022	40.0	37.0	40.0	33.0	40.0
40-41	37.91111130414754	40.0	37.0	40.0	33.0	40.0
42-43	37.814942660955914	40.0	37.0	40.0	33.0	40.0
44-45	37.76673812093205	40.0	37.0	40.0	33.0	40.0
46-47	37.73437698378376	40.0	37.0	40.0	33.0	40.0
48-49	37.707897572140624	40.0	37.0	40.0	33.0	40.0
50-51	37.637202672798	40.0	37.0	40.0	33.0	40.0
52-53	37.54465554367733	40.0	37.0	40.0	33.0	40.0
54-55	37.33699101227504	40.0	37.0	40.0	33.0	40.0
56-57	37.15102821496228	40.0	37.0	40.0	33.0	40.0
58-59	37.09744644552242	37.0	37.0	40.0	33.0	40.0
60-61	36.96742375048156	37.0	37.0	40.0	33.0	40.0
62-63	36.79828684455679	37.0	37.0	40.0	33.0	40.0
64-65	36.66458051865129	37.0	37.0	40.0	33.0	40.0
66-67	36.45363259496291	37.0	37.0	40.0	33.0	40.0
68-69	36.23424229592262	37.0	37.0	40.0	33.0	40.0
70-71	35.98604083876156	37.0	37.0	38.5	33.0	40.0
72-73	35.78417013035386	37.0	33.0	37.0	33.0	40.0
74-75	35.59866478292816	37.0	33.0	37.0	33.0	40.0
76-77	33.79153771762472	35.0	33.0	37.0	27.0	37.0
78-79	35.015096609421576	37.0	33.0	37.0	33.0	37.0
80-81	35.07292988940593	37.0	33.0	37.0	33.0	37.0
82-83	34.9518301687573	37.0	33.0	37.0	33.0	37.0
84-85	34.802905639656046	37.0	33.0	37.0	33.0	37.0
86-87	34.67605518036802	37.0	33.0	37.0	33.0	37.0
88-89	34.598474859981906	37.0	33.0	37.0	33.0	37.0
90-91	34.52231793366292	37.0	33.0	37.0	33.0	37.0
92-93	34.46460596299963	37.0	33.0	37.0	33.0	37.0
94-95	34.439108684642626	37.0	33.0	37.0	33.0	37.0
96-97	34.444061818922634	37.0	33.0	37.0	33.0	37.0
98-99	34.457425114244	37.0	33.0	37.0	33.0	37.0
100	34.460382027807746	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	14.0
20	89.0
21	484.0
22	1661.0
23	4687.0
24	10659.0
25	21064.0
26	36299.0
27	56982.0
28	83264.0
29	115807.0
30	155289.0
31	205031.0
32	268438.0
33	353976.0
34	480297.0
35	691623.0
36	1186765.0
37	2753322.0
38	3208085.0
39	70864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.29871026476333	20.572732504491377	14.440593505933464	23.68796372481183
2	14.369617623616588	20.81692333770063	42.53228717410782	22.281171864574965
3	17.955970840947767	26.629073217578487	30.60303224054724	24.811923700926506
4	12.11088848141185	18.346276368009125	39.40857553114701	30.13425961943202
5	13.034306555428527	38.16098480067143	35.75288481205766	13.051823831842391
6	29.228749115634873	38.33959043770741	19.105099775346012	13.326560671311698
7	26.01102248857642	32.987246907556695	23.34019426659543	17.661536337271457
8	24.096415089381903	37.1167696493608	21.200984470934458	17.585830790322838
9	25.43908341366378	16.8767417453699	21.433923030117867	36.25025181084845
10-11	24.185889524593055	26.912971432263365	28.922147747412062	19.978991295731518
12-13	25.105700307331148	24.41151966033702	28.95060356713517	21.532176465196656
14-15	22.101428982672765	25.790546301955953	26.86995153494154	25.238073180429744
16-17	21.552896249808725	28.805842114726826	27.47561620883891	22.165645426625538
18-19	21.46644333856619	27.95107630783213	29.32912437832989	21.253355975271788
20-21	22.45912949034616	27.109366547030582	29.196990446631077	21.23451351599218
22-23	22.115744616298798	27.19729198770629	28.905087967734534	21.781875428260378
24-25	21.80990112460563	27.32228437190729	29.125050706024215	21.74276379746286
26-27	21.886975824368214	27.45692743660541	29.000292973286022	21.655803765740355
28-29	21.682975881856763	27.60321712775754	29.04475112490907	21.669055865476633
30-31	21.96522927411615	27.346200180857615	29.068889883272558	21.619680661753673
32-33	21.74228720541982	27.598652756996405	28.9022423827828	21.75681765480098
34-35	21.87540688076877	27.626325052378537	28.896305778983557	21.601962287869135
36-37	21.87549916426012	27.5946623151833	28.867698648421563	21.66213987213502
38-39	21.743845773799226	27.669301832317384	28.79975154241722	21.787100851466178
40-41	22.004709605105223	27.54581107394396	28.774454618497575	21.675024702453246
42-43	22.039824407891352	27.58776716713289	28.52852846543884	21.84387995953692
44-45	22.017044418778724	27.844749593852146	28.237819765147044	21.90038622222208
46-47	22.061046861671638	27.699972112450727	27.96667839600352	22.272302629874115
48-49	22.076062028235768	27.843788333396358	27.95531973674668	22.124829901621194
50-51	21.949831158252717	28.00148008226534	27.786793815695283	22.261894943786665
52-53	22.032460637802647	27.909362720706266	27.724417008913182	22.333759632577905
54-55	22.163287142572393	27.72905416826007	27.776277257970307	22.331381431197237
56-57	22.167046479471107	27.70720360006273	27.70021101875784	22.425538901708318
58-59	21.923807316593198	27.666533230407133	28.01171538501972	22.397944067979946
60-61	22.14135787241473	27.76601668328181	27.987954105741302	22.10467133856216
62-63	21.84697244774797	27.929264847546282	27.881332779788874	22.342429924916875
64-65	21.679417165405642	28.099212768013082	27.77890510611451	22.44246496046676
66-67	21.840223727740995	28.345169739165666	27.453724177936433	22.360882355156907
68-69	21.884815233999877	28.26609607655347	27.411926862910914	22.437161826535736
70-71	21.83253743815802	27.80343028494874	27.394274024593052	22.96975825230019
72-73	21.994855732865926	27.595523747616735	27.476858718048263	22.932761801469077
74-75	22.06356466754228	27.697428700798284	27.47106052653802	22.767946105121414
76-77	22.286010623986115	27.572143833768774	27.118314823691808	23.0235307185533
78-79	22.366161891356583	27.62899304884869	26.846702014987972	23.15814304480675
80-81	22.28481049359121	27.76557938299028	27.000542004778637	22.949068118639875
82-83	22.334607478580953	27.568027239335763	27.179498179240547	22.917867102842738
84-85	22.494171291900145	27.76561936625328	26.9895320631277	22.75067727871887
86-87	22.529368590164474	27.784488460810753	27.08161051111721	22.60453243790756
88-89	22.565318601957472	27.7349776563469	27.226300182665824	22.473403559029805
90-91	22.525024957925396	27.541596022695032	27.255326591835498	22.67805242754407
92-93	22.55852833936646	27.237201382865372	27.409491067036328	22.794779210731836
94-95	21.962649270319403	27.565393243456192	27.805385197816403	22.666572288408002
96-97	22.119018340714717	27.58713820175263	27.824781162928662	22.46906229460399
98-99	23.287174470980936	29.402584413250395	28.192821579445738	19.117419536322934
100	22.253077267138146	30.60428113872577	21.819169803120868	25.323471791015216
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1089.0
1	1118.5
2	2271.0
3	3562.5
4	5276.5
5	9565.5
6	12917.0
7	14425.5
8	14435.0
9	12925.5
10	11102.0
11	9488.0
12	10559.0
13	15323.5
14	26382.0
15	41767.5
16	54123.0
17	61178.5
18	62847.0
19	59397.5
20	52494.0
21	44351.0
22	38634.0
23	38020.0
24	42339.0
25	52011.5
26	66977.5
27	84880.0
28	104248.0
29	126260.5
30	146920.5
31	166798.5
32	189694.0
33	213253.5
34	238232.5
35	261308.5
36	285896.0
37	312625.5
38	328262.0
39	336357.0
40	348496.5
41	359055.0
42	364618.0
43	373322.5
44	382609.0
45	391271.5
46	396589.5
47	396893.0
48	398542.0
49	395564.0
50	386477.0
51	374289.5
52	357210.0
53	337236.0
54	316144.5
55	288933.0
56	260017.5
57	231659.5
58	202499.0
59	174383.5
60	142338.0
61	110893.0
62	84221.5
63	62286.0
64	45292.5
65	32688.5
66	24108.5
67	18138.0
68	13780.0
69	10051.0
70	6994.0
71	4812.5
72	3338.5
73	2185.5
74	1372.5
75	879.0
76	619.0
77	422.0
78	275.0
79	190.0
80	129.0
81	100.5
82	80.5
83	51.0
84	28.5
85	18.0
86	10.0
87	6.5
88	4.5
89	3.5
90	2.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	3.0912840730346775E-5
7	0.0
8	0.0
9	0.0
10-11	0.001607467717978032
12-13	2.7306342645139653E-4
14-15	0.007249061151266318
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	163.0
20-21	699.0
22-23	1530.0
24-25	2621.0
26-27	3629.0
28-29	6494.0
30-31	12756.0
32-33	17263.0
34-35	24049.0
36-37	31134.0
38-39	28395.0
40-41	43642.0
42-43	53787.0
44-45	65689.0
46-47	63357.0
48-49	55377.0
50-51	32618.0
52-53	16327.0
54-55	16634.0
56-57	16744.0
58-59	38266.0
60-61	63952.0
62-63	62908.0
64-65	67342.0
66-67	74327.0
68-69	72136.0
70-71	72033.0
72-73	69439.0
74-75	72171.0
76-77	70887.0
78-79	77825.0
80-81	81991.0
82-83	79917.0
84-85	81932.0
86-87	86713.0
88-89	95745.0
90-91	101916.0
92-93	108376.0
94-95	123943.0
96-97	349140.0
98-99	660331.0
100-101	6700507.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.4782052234659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.33616112220854	64.4987823806355
2	9.725602691375185	14.875932771057677
3	3.001726058937225	6.886998646800797
4	1.2998215578465315	3.9763207941948875
5	0.6208246865138637	2.3739778891500576
6	0.3499678327603961	1.60589470412767
7	0.20858728626711934	1.1166666900299809
8	0.12765104894849566	0.7810018494778994
9	0.08775601330312865	0.604028015549089
>10	0.2367447465035433	2.614600915685285
>50	0.003073607717763986	0.15732164080087396
>100	0.0017225703906855539	0.2229220681042374
>500	2.2201690490539764E-4	0.13101764542820443
>1k	1.3876032263554345E-4	0.15453398895789078
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	6.182568146069355E-5	0.0	0.0	0.0
5	0.0	6.182568146069355E-5	0.0	0.0	0.0
6	0.0	6.182568146069355E-5	0.0	0.0	0.0
7	0.0	6.182568146069355E-5	0.0	1.0304280243448925E-5	0.0
8	0.0	6.182568146069355E-5	0.0	1.0304280243448925E-5	0.0
9	0.0	1.7517276413863173E-4	0.0	1.0304280243448925E-5	0.0
10-11	0.0	1.7517276413863173E-4	0.0	2.060856048689785E-5	0.0
12-13	0.0	1.7517276413863173E-4	0.0	4.12171209737957E-5	0.0
14-15	0.0	2.318463054776008E-4	0.0	7.728210182586693E-5	0.0
16-17	0.0	2.3699844559932527E-4	0.0	1.081949425562137E-4	0.0
18-19	0.0	2.7821556657312094E-4	0.0	1.7002062401690727E-4	0.0
20-21	0.0	3.1943268754691667E-4	0.0	2.060856048689785E-4	0.0
22-23	0.0	3.4004124803381454E-4	0.0	2.5760700608622313E-4	0.0
24-25	0.0	3.7095408876416133E-4	0.0	4.843011714420995E-4	0.0
26-27	0.0	3.812583690076102E-4	0.0	0.001112862266292484	0.0
28-29	0.0	3.812583690076102E-4	0.0	0.003379803919851247	0.0
30-31	0.0	4.3277977022485487E-4	0.0	0.005672506274018633	0.0
32-33	0.0	4.430840504683038E-4	0.0	0.012159050687269732	0.0
34-35	0.0	5.255182924158952E-4	0.0	0.02084040679237545	0.0
36-37	0.0	6.182568146069355E-4	0.0	0.03605467657182779	0.0
38-39	0.0	6.491696553372823E-4	0.0	0.11342436477976404	0.0
40-41	0.0	7.831252985021183E-4	0.0	0.1971981631590038	0.0
42-43	0.0	8.24342419475914E-4	0.0	0.22930630039759065	0.0
44-45	0.0	9.995151836145457E-4	0.0	0.2704718999701691	0.0
46-47	0.0	0.0011334708267793819	0.0	0.3132913365218211	0.0
48-49	0.0	0.001159231527388004	0.0	0.35580164466616965	0.0
50-51	0.0	0.0013550128520135336	0.0	0.39591620765391633	0.0
52-53	0.0	0.001370469272378707	0.0	0.6717308769303136	0.0
54-55	0.0	0.001370469272378707	0.0	1.0475228252687743	0.0
56-57	0.0	0.001370469272378707	0.0	1.4733059892083273	0.0
58-59	0.0	0.0013756214125004314	0.0	1.9374416842139972	0.0
60-61	0.0	0.0014116863933525028	0.0	2.0688882351395534	0.0
62-63	0.0	0.0014425992340828496	0.0	2.0749007826616057	0.0
64-65	0.0	0.0016280762784649302	0.0	2.080377507610999	0.0
66-67	0.0	0.0016435326988301037	0.0	2.0841643306004665	0.0
68-69	0.0	0.0016847498198038994	0.0	2.0877708286856738	0.0
70-71	0.0	0.0017002062401690727	0.0	2.0914391524523417	0.0
72-73	0.0	0.0017002062401690727	0.0	2.0944686108439154	0.0
74-75	0.0	0.0017517276413863173	0.0	2.0968746602807604	0.0
76-77	0.0	0.0018238576030904599	0.0	2.0994558824817444	0.0
78-79	0.0	0.0018547704438208064	0.0	2.1008624167349756	0.0
80-81	0.0	0.0018547704438208064	0.0	2.101336413626174	0.0
82-83	0.0	0.001859922583942531	0.0	2.101697063434695	0.0
84-85	0.0	0.0018650747240642554	0.0	2.1018876926191985	0.0
86-87	0.0	0.0018650747240642554	0.0	2.101985583281511	0.0
88	0.0	0.0019578132462552958	0.0	2.1020010397018765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16115	0.0	44.028835	1
GTATCAA	26320	0.0	34.179607	1
ATCAACG	31755	0.0	27.557251	3
TCAACGC	31880	0.0	27.531631	4
CAACGCA	32180	0.0	27.235117	5
AACGCAG	32815	0.0	26.828218	6
TATCAAC	33095	0.0	26.812023	2
ACGCAGA	36980	0.0	23.711845	7
CGCAGAG	37160	0.0	23.574022	8
GTACATG	33195	0.0	21.77027	1
TACATGG	33135	0.0	21.16201	2
ACATGGG	32955	0.0	20.626377	3
GCAGAGT	43200	0.0	20.220316	9
GAGTACT	24715	0.0	20.01965	12-13
ATCTCGT	9125	0.0	20.007473	94
CATGGGG	23160	0.0	19.005533	4
AGAGTAC	39325	0.0	18.542213	10-11
CAGAGTA	42320	0.0	18.539433	10-11
TATCTCG	10605	0.0	18.530352	94
GTACTAG	2750	0.0	17.837662	1
>>END_MODULE