FastQCFastQC Report
Fri 27 May 2016
SRR2139104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9616948
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118800.046.0641561
GTATCAA206150.034.6040041
TCAACGC232200.029.827594
ATCAACG238550.029.0517393
CAACGCA239300.028.9974465
AACGCAG246950.028.0816466
TATCAAC250600.027.9482632
CTTATAC132150.026.7778171
ACGCAGA282750.024.5110827
CGCAGAG291200.023.7998248
TATACAC170100.023.3468023
GTACATG288400.022.9351351
TTATACA167350.022.2052922
TACATGG293250.022.1870142
ACATGGG297100.021.346193
GCAGAGT343950.020.0146269
CATGGGG234250.019.4094054
TACACAT200250.019.2271795
GAGTACT185450.019.16656512-13
GTGGTAT64400.018.3385851
Produced by FastQC (version 0.11.5)