##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9616948
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55516916593497	33.0	33.0	33.0	33.0	33.0
2	32.564404736305114	33.0	33.0	33.0	33.0	33.0
3	32.558166894528284	33.0	33.0	33.0	33.0	33.0
4	36.45330472827762	37.0	37.0	37.0	37.0	37.0
5	36.47824923250079	37.0	37.0	37.0	37.0	37.0
6	36.47738346926697	37.0	37.0	37.0	37.0	37.0
7	36.45372918726398	37.0	37.0	37.0	37.0	37.0
8	36.43486894178902	37.0	37.0	37.0	37.0	37.0
9	36.47511913342986	37.0	37.0	37.0	37.0	37.0
10-11	36.42817388635147	37.0	37.0	37.0	37.0	37.0
12-13	36.3581470441558	37.0	37.0	37.0	37.0	37.0
14-15	38.374861130579056	40.0	37.0	40.0	37.0	40.0
16-17	38.32721976868337	40.0	37.0	40.0	37.0	40.0
18-19	38.329745777974466	40.0	37.0	40.0	37.0	40.0
20-21	38.32534576102722	40.0	37.0	40.0	37.0	40.0
22-23	38.317324586277515	40.0	37.0	40.0	37.0	40.0
24-25	38.31484012455558	40.0	37.0	40.0	37.0	40.0
26-27	38.15801230662147	40.0	37.0	40.0	35.0	40.0
28-29	38.07390407976516	40.0	37.0	40.0	33.0	40.0
30-31	38.046620071228276	40.0	37.0	40.0	33.0	40.0
32-33	37.96595238519683	40.0	37.0	40.0	33.0	40.0
34-35	37.91859463065956	40.0	37.0	40.0	33.0	40.0
36-37	37.87698821228267	40.0	37.0	40.0	33.0	40.0
38-39	37.81066119781204	40.0	37.0	40.0	33.0	40.0
40-41	37.78678102573298	40.0	37.0	40.0	33.0	40.0
42-43	37.694833613013486	40.0	37.0	40.0	33.0	40.0
44-45	37.64209051001222	40.0	37.0	40.0	33.0	40.0
46-47	37.602084063603755	40.0	37.0	40.0	33.0	40.0
48-49	37.578537026566934	40.0	37.0	40.0	33.0	40.0
50-51	37.490851953402725	40.0	37.0	40.0	33.0	40.0
52-53	37.389703290727724	40.0	37.0	40.0	33.0	40.0
54-55	37.169849457700394	40.0	37.0	40.0	33.0	40.0
56-57	36.97275656465564	37.0	37.0	40.0	33.0	40.0
58-59	36.906175609821545	37.0	37.0	40.0	33.0	40.0
60-61	36.75934473903516	37.0	37.0	40.0	33.0	40.0
62-63	36.57165180390307	37.0	37.0	40.0	33.0	40.0
64-65	36.423226107738486	37.0	37.0	40.0	33.0	40.0
66-67	36.20339798356207	37.0	37.0	40.0	33.0	40.0
68-69	35.9843576295855	37.0	37.0	40.0	33.0	40.0
70-71	35.7242547137214	37.0	33.0	37.0	33.0	40.0
72-73	35.51903003882987	37.0	33.0	37.0	33.0	40.0
74-75	35.34614922443373	37.0	33.0	37.0	33.0	40.0
76-77	33.55173820248429	35.0	33.0	37.0	27.0	37.0
78-79	34.81654240955025	37.0	33.0	37.0	30.0	37.0
80-81	34.90188491699284	37.0	33.0	37.0	33.0	37.0
82-83	34.79778695999765	37.0	33.0	37.0	33.0	37.0
84-85	34.65523822760243	37.0	33.0	37.0	33.0	37.0
86-87	34.53978371412228	37.0	33.0	37.0	33.0	37.0
88-89	34.47304118514367	37.0	33.0	37.0	30.0	37.0
90-91	34.39616910876657	37.0	33.0	37.0	30.0	37.0
92-93	34.33145270673644	37.0	33.0	37.0	27.0	37.0
94-95	34.32551727484281	37.0	33.0	37.0	27.0	37.0
96-97	34.33872285155785	37.0	33.0	37.0	33.0	37.0
98-99	34.35040854865292	37.0	33.0	37.0	33.0	37.0
100	34.36076745502358	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	13.0
20	91.0
21	559.0
22	2039.0
23	5556.0
24	12333.0
25	23594.0
26	40403.0
27	62977.0
28	90762.0
29	124435.0
30	166195.0
31	216157.0
32	282770.0
33	372211.0
34	503518.0
35	721254.0
36	1232637.0
37	2767322.0
38	2929796.0
39	62324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32368189991253	20.07374896900763	14.3060043581394	24.29656477294044
2	14.690284277298785	20.462916093546518	41.81512679490416	23.031672834250532
3	18.440184973444797	25.66896483167009	30.174489869343162	25.71636032554195
4	12.758631948514227	17.907458790460343	37.86740866229078	31.466500598734648
5	13.836863836635075	37.39449355450399	35.12536409680077	13.643278512060167
6	30.654052684287436	37.14035233842923	18.378410528829992	13.82718444845334
7	26.98922776747883	32.20875271447865	22.450750487576723	18.351269030465797
8	24.66031842950591	36.86687294139471	20.619888971012426	17.852919658086954
9	25.572385334723656	16.852539911830654	20.670507940772893	36.9045668126728
10-11	24.780386949880732	26.38749621625736	28.2768875013635	20.555229332498406
12-13	25.520028924030434	23.750553321818206	28.402496350961542	22.326921403189818
14-15	22.62591788108395	25.188734159280074	25.754605166521728	26.43074279311425
16-17	22.406271719468588	28.445781343519794	26.441288857962007	22.70665807904961
18-19	22.47034090233201	27.477121639838337	28.021155984206214	22.03138147362344
20-21	23.183520194212335	26.66299145932837	27.885397378370584	22.26809096808871
22-23	23.00825168620464	26.774464032452666	27.73899971027376	22.478284571068933
24-25	22.864484917372327	27.031991914521253	27.73063028270572	22.372892885400695
26-27	22.81811215979042	27.06693588759227	27.74781935160055	22.367132601016763
28-29	22.624543731466	26.872396366527056	27.837473210959455	22.665586691047483
30-31	23.02642544910554	26.901348879267594	27.728506273517876	22.343719398108984
32-33	22.845776200822954	27.010610009686253	27.80269154222756	22.340922247263233
34-35	22.837781729103263	26.999935008265663	27.869172004781678	22.2931112578494
36-37	22.896800532399332	26.87141810376951	27.670546813321582	22.56123455050957
38-39	22.662130246300467	26.852533909512232	27.932779807037093	22.552556037150204
40-41	22.792454771368927	26.716889652957672	27.883040107472045	22.607615468201352
42-43	22.807854909387526	26.827997623296802	27.72066044855937	22.643487018756304
44-45	22.738950357640782	26.971970081422942	27.57325770920312	22.715821851733164
46-47	22.952238410755964	26.858853633881147	27.225677810994362	22.963230144368524
48-49	22.74203739760143	27.061298515947346	27.19384256604326	23.002821520407963
50-51	22.66359198419596	27.139633144259918	27.29442143839995	22.902353433144174
52-53	22.845588532102912	27.123159399826292	27.244806585094405	22.786445482976397
54-55	22.829164683388104	27.02827675636554	27.11812769813795	23.024430862108407
56-57	22.937900271173703	27.202390460886317	26.940852484363347	22.91885678357663
58-59	22.699674891394327	27.383458695459577	27.15910721303848	22.75775920010761
60-61	22.953517224357995	27.446582672951592	26.899246532555015	22.700653570135398
62-63	22.85492702032249	27.609918191541805	26.80722732272052	22.72792746541518
64-65	22.79969705999974	27.476277428430766	26.802967885734784	22.92105762583471
66-67	22.929830821688448	27.410985086026102	26.660969776936007	22.998214315349447
68-69	22.794528123255475	27.323993039696248	26.82848264561542	23.052996191432854
70-71	22.851225949697465	27.15795819640192	26.838609879647397	23.152205974253217
72-73	22.935070514892395	27.136168657740615	26.728579434153023	23.200181393213963
74-75	22.77964591096386	27.330953869163423	26.751555658674437	23.137844561198285
76-77	23.029683954235296	27.23925257230927	26.660444570536164	23.07061890291927
78-79	22.930837190389376	27.212582586531287	26.63235926414103	23.224220958938304
80-81	22.980045572870754	27.261392282536832	26.66182799592507	23.09673414866734
82-83	22.94059445042757	27.279973129777947	26.745440920362057	23.033991499432425
84-85	23.035250723031034	27.259753936384268	26.696806341653552	23.008188998931153
86-87	22.941397395522518	27.25812030972199	26.789711575168766	23.01077071958673
88-89	22.957455135513232	27.21836133831537	26.77221711468199	23.051966411489406
90-91	22.95287807008726	27.29902346685612	26.73208783826872	23.0160106247879
92-93	23.10514815908724	27.191783571385447	26.7698398064035	22.933228463123818
94-95	22.84737461246686	27.29666290642394	26.87763985810536	22.978322623003834
96-97	22.92896628784576	27.365353990172814	26.937881271946296	22.767798450035134
98-99	24.150235125939034	29.269174310083518	26.99215865606373	19.588431907913716
100	23.017610973197876	30.242377755705512	20.867327328536483	25.872683942560133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1593.0
1	1393.5
2	1839.0
3	2631.5
4	3547.0
5	5820.5
6	7845.0
7	9175.0
8	9812.5
9	9565.0
10	8806.5
11	7923.5
12	8498.0
13	11209.5
14	17994.0
15	27848.0
16	36269.5
17	41614.5
18	43381.0
19	41626.5
20	37792.5
21	33485.5
22	30944.5
23	31797.5
24	36115.5
25	45077.5
26	57521.0
27	70869.0
28	86210.0
29	105012.5
30	124399.5
31	144509.0
32	167501.0
33	191247.5
34	212366.0
35	230599.0
36	253006.0
37	276699.5
38	294591.5
39	306705.0
40	320322.0
41	335957.5
42	346223.0
43	356074.5
44	368255.0
45	378278.0
46	385030.0
47	390765.5
48	405937.0
49	414352.5
50	408018.0
51	405294.0
52	399548.5
53	385226.0
54	367037.0
55	344352.5
56	320186.0
57	294145.5
58	263803.0
59	228949.0
60	190053.0
61	153783.0
62	122571.5
63	94594.0
64	69642.5
65	51071.0
66	37945.0
67	28510.0
68	21246.5
69	15246.0
70	10757.5
71	7536.5
72	5166.5
73	3383.0
74	2159.5
75	1368.5
76	925.0
77	609.0
78	418.5
79	295.5
80	199.5
81	143.0
82	117.5
83	95.0
84	70.5
85	50.5
86	37.0
87	29.5
88	21.0
89	13.5
90	8.0
91	4.5
92	4.0
93	2.5
94	1.5
95	2.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0398309318091353E-5
7	0.0
8	0.0
9	0.0
10-11	0.0015285514697594288
12-13	3.2234758886083196E-4
14-15	0.007387998770503905
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	158.0
20-21	570.0
22-23	1192.0
24-25	2923.0
26-27	3796.0
28-29	5916.0
30-31	14904.0
32-33	18963.0
34-35	26293.0
36-37	39040.0
38-39	43825.0
40-41	47637.0
42-43	54604.0
44-45	65694.0
46-47	63589.0
48-49	60024.0
50-51	51605.0
52-53	49880.0
54-55	57324.0
56-57	64619.0
58-59	65613.0
60-61	69715.0
62-63	68673.0
64-65	72064.0
66-67	79161.0
68-69	70699.0
70-71	69376.0
72-73	66350.0
74-75	69310.0
76-77	66587.0
78-79	73839.0
80-81	76852.0
82-83	75296.0
84-85	75792.0
86-87	80713.0
88-89	87231.0
90-91	92591.0
92-93	99323.0
94-95	121712.0
96-97	348412.0
98-99	608174.0
100-101	6506909.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.30583378869805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.92569526660596	74.08029189424289
2	7.626271841837615	12.70625868967105
3	1.8554869606569566	4.637186650247549
4	0.6995562973934111	2.331084825459701
5	0.3336763709806717	1.3898594150065895
6	0.1799725155670992	0.8995656281019985
7	0.09784211094672587	0.570557304244431
8	0.07054874985506135	0.4701697943540941
9	0.041210609462851794	0.30897757640189505
>10	0.16371141263985103	2.141545543652451
>50	0.004406403145334519	0.24595755605921668
>100	0.0015982982172594025	0.20775872948639867
>500	2.3162691300466154E-5	0.010786393071707355
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0398309318091353E-5	0.0
2	0.0	1.0398309318091353E-5	0.0	1.0398309318091353E-5	0.0
3	0.0	1.0398309318091353E-5	0.0	1.0398309318091353E-5	0.0
4	0.0	1.0398309318091353E-5	0.0	1.0398309318091353E-5	0.0
5	0.0	1.0398309318091353E-5	0.0	2.0796618636182705E-5	0.0
6	6.238985590854811E-5	1.0398309318091353E-5	0.0	2.0796618636182705E-5	1.0398309318091353E-5
7	6.238985590854811E-5	1.0398309318091353E-5	0.0	2.0796618636182705E-5	1.0398309318091353E-5
8	6.238985590854811E-5	2.0796618636182705E-5	0.0	2.0796618636182705E-5	1.0398309318091353E-5
9	6.238985590854811E-5	3.1194927954274056E-5	0.0	2.0796618636182705E-5	1.0398309318091353E-5
10-11	6.238985590854811E-5	3.1194927954274056E-5	0.0	4.159323727236541E-5	1.0398309318091353E-5
12-13	6.238985590854811E-5	3.6394082613319734E-5	0.0	4.159323727236541E-5	1.5597463977137028E-5
14-15	6.238985590854811E-5	5.1991546590456765E-5	0.0	5.7190701249502435E-5	3.6394082613319734E-5
16-17	7.798731988568514E-5	5.1991546590456765E-5	0.0	7.278816522663947E-5	5.1991546590456765E-5
18-19	8.318647454473082E-5	6.238985590854811E-5	0.0	1.0398309318091353E-4	8.318647454473082E-5
20-21	8.318647454473082E-5	7.278816522663947E-5	0.0	1.3517802113518758E-4	1.2997886647614192E-4
22-23	8.83856292037765E-5	1.0398309318091353E-4	0.0	1.559746397713703E-4	1.4037717579423324E-4
24-25	9.358478386282218E-5	1.0918224783995921E-4	0.0	2.8075435158846654E-4	1.6117379443041595E-4
26-27	9.358478386282218E-5	1.1438140249900488E-4	0.0	6.031019404492985E-4	1.6637294908946164E-4
28-29	9.358478386282218E-5	1.1438140249900488E-4	0.0	0.0015389497790775203	1.76771258407553E-4
30-31	9.358478386282218E-5	1.3517802113518758E-4	0.0	0.005682676042336924	1.8716956772564435E-4
32-33	9.358478386282218E-5	1.3517802113518758E-4	0.0	0.015805430163498856	1.8716956772564435E-4
34-35	9.358478386282218E-5	1.5077548511232462E-4	0.0	0.028808515965772094	1.975678770437357E-4
36-37	9.878393852186785E-5	1.6637294908946164E-4	0.0	0.04827415100923911	1.975678770437357E-4
38-39	1.0398309318091353E-4	1.8716956772564435E-4	0.0	0.08806848076957471	2.0796618636182706E-4
40-41	1.1438140249900488E-4	2.1836449567991841E-4	0.0	0.13022322674511705	2.1836449567991841E-4
42-43	1.1438140249900488E-4	2.3396195965705543E-4	0.0	0.160835849377578	2.2356365033896408E-4
44-45	1.1958055715805056E-4	2.9115266090655787E-4	0.0	0.19689198693805976	2.2876280499800977E-4
46-47	1.3517802113518758E-4	3.327458981789233E-4	0.0	0.23075408123242425	2.2876280499800977E-4
48-49	1.4037717579423324E-4	3.37945052837969E-4	0.0	0.262822467169418	2.2876280499800977E-4
50-51	1.559746397713703E-4	4.0553406340556274E-4	0.0	0.2909446947201961	2.2876280499800977E-4
52-53	1.559746397713703E-4	4.159323727236541E-4	0.0	0.34256190217520155	2.3916111431610112E-4
54-55	1.559746397713703E-4	4.263306820417455E-4	0.0	0.39699185230075074	2.4955942363419245E-4
56-57	1.6117379443041595E-4	4.3672899135983683E-4	0.0	0.4485726656731429	2.5475857829323817E-4
58-59	1.76771258407553E-4	4.3672899135983683E-4	0.0	0.48084381864183934	2.5995773295228383E-4
60-61	1.9236872238469004E-4	4.419281460188825E-4	0.0	0.5039800568745927	2.5995773295228383E-4
62-63	2.2876280499800977E-4	4.627247646550652E-4	0.0	0.5207369323406968	2.5995773295228383E-4
64-65	2.2876280499800977E-4	5.719070124950244E-4	0.0	0.5262480362792852	2.5995773295228383E-4
66-67	2.3916111431610112E-4	5.719070124950244E-4	0.0	0.5311716357414016	2.651568876113295E-4
68-69	2.3916111431610112E-4	5.875044764721614E-4	0.0	0.5359028664811332	2.7035604227037516E-4
70-71	2.4955942363419245E-4	5.979027857902527E-4	0.0	0.5398906181046211	2.8075435158846654E-4
72-73	2.4955942363419245E-4	6.031019404492985E-4	0.0	0.543181683003797	2.9115266090655787E-4
74-75	2.5475857829323817E-4	6.290977137445268E-4	0.0	0.5457864594879789	2.9115266090655787E-4
76-77	2.5995773295228383E-4	6.498943323807096E-4	0.0	0.5481780706311399	2.963518155656036E-4
78-79	2.5995773295228383E-4	6.654917963578466E-4	0.0	0.5497534144928308	3.0155097022464925E-4
80-81	2.5995773295228383E-4	6.706909510168922E-4	0.0	0.5502421350307811	3.119492795427406E-4
82-83	2.5995773295228383E-4	6.758901056759379E-4	0.0	0.5508400378165713	3.53542516815106E-4
84-85	2.5995773295228383E-4	6.758901056759379E-4	0.0	0.551016809074979	3.743391354512887E-4
86-87	2.7035604227037516E-4	6.758901056759379E-4	0.0	0.5510999955495236	3.8473744476938003E-4
88	2.7035604227037516E-4	7.07085033630212E-4	0.0	0.5510999955495236	3.8473744476938003E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11880	0.0	46.064156	1
GTATCAA	20615	0.0	34.604004	1
TCAACGC	23220	0.0	29.82759	4
ATCAACG	23855	0.0	29.051739	3
CAACGCA	23930	0.0	28.997446	5
AACGCAG	24695	0.0	28.081646	6
TATCAAC	25060	0.0	27.948263	2
CTTATAC	13215	0.0	26.777817	1
ACGCAGA	28275	0.0	24.511082	7
CGCAGAG	29120	0.0	23.799824	8
TATACAC	17010	0.0	23.346802	3
GTACATG	28840	0.0	22.935135	1
TTATACA	16735	0.0	22.205292	2
TACATGG	29325	0.0	22.187014	2
ACATGGG	29710	0.0	21.34619	3
GCAGAGT	34395	0.0	20.014626	9
CATGGGG	23425	0.0	19.409405	4
TACACAT	20025	0.0	19.227179	5
GAGTACT	18545	0.0	19.166565	12-13
GTGGTAT	6440	0.0	18.338585	1
>>END_MODULE