FastQCFastQC Report
Fri 27 May 2016
SRR2139103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11885824
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147700.046.7462041
GTATCAA262850.036.117821
TCAACGC305300.030.4140074
ATCAACG311550.029.7760313
CAACGCA316350.029.3115145
TATCAAC322800.029.0338762
AACGCAG323250.028.7260916
CTTATAC113950.028.5069851
ACGCAGA377350.024.5617087
CGCAGAG380300.024.348688
TATACAC163350.023.5233043
GTACATG364750.022.365431
TACATGG364250.021.9674992
TTATACA157700.021.3959562
ACATGGG371050.021.0506293
GCAGAGT448300.020.6488259
GAGTACT245300.019.26569612-13
CATGGGG281950.018.442974
CAGAGTA433250.018.40749510-11
AGAGTAC399150.018.21952610-11
Produced by FastQC (version 0.11.5)