##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11885824
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56118406262788	33.0	33.0	33.0	33.0	33.0
2	32.57069068160525	33.0	33.0	33.0	33.0	33.0
3	32.564256630419564	33.0	33.0	33.0	33.0	33.0
4	36.46122027383209	37.0	37.0	37.0	37.0	37.0
5	36.488172633214155	37.0	37.0	37.0	37.0	37.0
6	36.48736292914988	37.0	37.0	37.0	37.0	37.0
7	36.46314979929031	37.0	37.0	37.0	37.0	37.0
8	36.446422982537854	37.0	37.0	37.0	37.0	37.0
9	36.4835520027892	37.0	37.0	37.0	37.0	37.0
10-11	36.43838563485376	37.0	37.0	37.0	37.0	37.0
12-13	36.370420679289886	37.0	37.0	37.0	37.0	37.0
14-15	38.39807564035947	40.0	37.0	40.0	37.0	40.0
16-17	38.356034087329576	40.0	37.0	40.0	37.0	40.0
18-19	38.36388390068707	40.0	37.0	40.0	37.0	40.0
20-21	38.35986126769379	40.0	37.0	40.0	37.0	40.0
22-23	38.35344039920294	40.0	37.0	40.0	37.0	40.0
24-25	38.351163670499346	40.0	37.0	40.0	37.0	40.0
26-27	38.19969902433739	40.0	37.0	40.0	37.0	40.0
28-29	38.12893652116297	40.0	37.0	40.0	33.0	40.0
30-31	38.10038544956009	40.0	37.0	40.0	33.0	40.0
32-33	38.02082254411846	40.0	37.0	40.0	33.0	40.0
34-35	37.97708736662558	40.0	37.0	40.0	33.0	40.0
36-37	37.94144159223359	40.0	37.0	40.0	33.0	40.0
38-39	37.88696775247228	40.0	37.0	40.0	33.0	40.0
40-41	37.8607166257047	40.0	37.0	40.0	33.0	40.0
42-43	37.76645575999033	40.0	37.0	40.0	33.0	40.0
44-45	37.71491076827378	40.0	37.0	40.0	33.0	40.0
46-47	37.68197359945741	40.0	37.0	40.0	33.0	40.0
48-49	37.65944479869526	40.0	37.0	40.0	33.0	40.0
50-51	37.58172664023908	40.0	37.0	40.0	33.0	40.0
52-53	37.48227070160611	40.0	37.0	40.0	33.0	40.0
54-55	37.266261257115445	40.0	37.0	40.0	33.0	40.0
56-57	37.07057543669792	37.0	37.0	40.0	33.0	40.0
58-59	37.004948986652465	37.0	37.0	40.0	33.0	40.0
60-61	36.86011201485407	37.0	37.0	40.0	33.0	40.0
62-63	36.67517068522211	37.0	37.0	40.0	33.0	40.0
64-65	36.53103884835289	37.0	37.0	40.0	33.0	40.0
66-67	36.31170620157625	37.0	37.0	40.0	33.0	40.0
68-69	36.09440748495986	37.0	37.0	40.0	33.0	40.0
70-71	35.83703864232521	37.0	35.0	37.0	33.0	40.0
72-73	35.63302781758077	37.0	33.0	37.0	33.0	40.0
74-75	35.45524659020364	37.0	33.0	37.0	33.0	40.0
76-77	33.657522784159156	35.0	33.0	37.0	27.0	37.0
78-79	34.91078589106155	37.0	33.0	37.0	33.0	37.0
80-81	34.988079424797675	37.0	33.0	37.0	33.0	37.0
82-83	34.88037452015982	37.0	33.0	37.0	33.0	37.0
84-85	34.733194927379046	37.0	33.0	37.0	33.0	37.0
86-87	34.615147505655884	37.0	33.0	37.0	33.0	37.0
88-89	34.544014293566256	37.0	33.0	37.0	33.0	37.0
90-91	34.467425157267314	37.0	33.0	37.0	33.0	37.0
92-93	34.40173710264396	37.0	33.0	37.0	30.0	37.0
94-95	34.39557669370049	37.0	33.0	37.0	33.0	37.0
96-97	34.40538344413557	37.0	33.0	37.0	33.0	37.0
98-99	34.415061600208574	37.0	33.0	37.0	33.0	37.0
100	34.42458554623211	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	21.0
20	98.0
21	521.0
22	2271.0
23	6173.0
24	14136.0
25	27763.0
26	47256.0
27	74095.0
28	106885.0
29	146749.0
30	194283.0
31	256284.0
32	335467.0
33	443153.0
34	603765.0
35	870380.0
36	1486405.0
37	3413100.0
38	3774322.0
39	82696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06797307447931	20.253421218419522	14.461967466454157	24.216638240647008
2	14.537999216545694	20.603325440457475	41.94200587186888	22.91666947112796
3	18.174760117598915	25.967177370285814	30.283419979969416	25.57464253214586
4	12.577419958431152	18.064082052704126	38.25779348575244	31.10070450311228
5	13.635924610695902	37.55382883004157	35.2836118051218	13.526634754140732
6	30.249180052571873	37.403594181067646	18.653996446018084	13.693229320342395
7	26.798217776066686	32.375416294234206	22.719577540438088	18.106788389261023
8	24.512570605117492	36.8615840180706	20.86956697322794	17.756278403583966
9	25.49126589793017	16.949392822912404	20.909656747399254	36.64968453175817
10-11	24.520235152661733	26.551101726490888	28.47637028900546	20.45229283184192
12-13	25.36588987725461	23.924491298742847	28.54854528929837	22.161073534704173
14-15	22.442317590088102	25.35990688727398	26.041024660686386	26.156750861951533
16-17	22.12931135443365	28.609728698658166	26.73342210014215	22.52753784676603
18-19	22.184360125137307	27.668401450332762	28.317767451377374	21.829470973152556
20-21	22.867042053253854	26.668182516127807	28.313536844186523	22.151238586431816
22-23	22.630657610761943	26.85026191200115	28.118855110362578	22.400225366874327
24-25	22.44779865978192	27.028948740828362	28.23414692275564	22.289105676634076
26-27	22.336952408835856	27.142262354170494	28.191072269916017	22.32971296707763
28-29	22.143798617108676	26.99111006855086	28.238413822900426	22.626677491440038
30-31	22.363389851421417	26.836861742129418	28.292256098501422	22.50749230794774
32-33	22.24557654382422	27.027724178521794	28.270385197610437	22.456314080043548
34-35	22.35490299803497	27.209978311511644	28.279171312840695	22.15594737761269
36-37	22.459772406809584	27.07198401875154	27.931987927725288	22.536255646713585
38-39	22.39649704136772	27.076301349702366	28.091312332741055	22.435889276188863
40-41	22.56191823358207	26.99336306877413	28.04182132254856	22.40289737509524
42-43	22.579422639173423	27.00080025906765	27.974083435153098	22.44569366660583
44-45	22.531390040269837	27.155444818457035	27.762191196689006	22.550973944584126
46-47	22.760840104859785	27.04287013060779	27.36048198841915	22.83580777611328
48-49	22.610444532252853	27.168348451046548	27.396173258948597	22.825033757752003
50-51	22.537304956176467	27.326516128129757	27.377334721857117	22.758844193836662
52-53	22.639213943385435	27.354649315649105	27.413509669160884	22.59262707180457
54-55	22.629667655827138	27.230656233161373	27.285512733786117	22.854163377225376
56-57	22.762858752746375	27.403468119376146	27.10181125760584	22.731861870271644
58-59	22.537515153267385	27.586658726994777	27.366105247526782	22.50972087221105
60-61	22.768187320356986	27.680282327993417	27.065383846595836	22.486146505053764
62-63	22.652645088742286	27.828724038552526	26.97750275841097	22.541128114294217
64-65	22.64996410349343	27.666497424183735	26.995311924575944	22.688226547746893
66-67	22.74004798694441	27.534814355354676	26.94041426455594	22.784723393144976
68-69	22.682273369148135	27.421203403307242	27.09761359107355	22.798909636471073
70-71	22.72557018117697	27.269093879677804	27.08005731672141	22.92527862242382
72-73	22.75885892983764	27.279695393098113	27.007394123934585	22.954051553129656
74-75	22.656199712373155	27.38283343057667	27.031807563591766	22.929159293458408
76-77	22.803460142428765	27.321913253624313	26.940714169555807	22.933912434391114
78-79	22.699522559197945	27.308449650668248	26.958534806805474	23.03349298332833
80-81	22.732075861960887	27.38467907310102	26.96440579907315	22.91883926586494
82-83	22.741424971129042	27.339136327797682	27.062529896184284	22.856908804888995
84-85	22.814023312312116	27.34124828750667	26.95324171406958	22.891486686111634
86-87	22.717514450752486	27.34481465495533	27.044692730900127	22.89297816339206
88-89	22.719393148721267	27.295190080754793	27.0361574245375	22.94925934598644
90-91	22.742393241233145	27.361130694647116	27.01887509077358	22.877600973346155
92-93	22.788316810343808	27.32802970265765	27.045127483065563	22.838526003932984
94-95	22.650304219007232	27.380897935227193	27.109441056838605	22.85935678892697
96-97	22.70581823771452	27.487188748306004	27.15154071390513	22.655452300074348
98-99	23.908193906424138	29.36629175447977	27.318954034896425	19.406560304199665
100	22.823342617255843	30.3383666126628	21.048193884271633	25.790096885809728
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1664.0
1	1501.5
2	2263.0
3	3365.0
4	4618.5
5	7834.5
6	10496.0
7	11897.0
8	12440.5
9	11889.0
10	10788.0
11	9528.0
12	10260.0
13	14103.0
14	23365.0
15	36555.5
16	47944.0
17	55258.0
18	57749.0
19	55391.0
20	49787.5
21	43238.0
22	38700.5
23	39135.0
24	44948.5
25	55432.5
26	70148.5
27	88275.0
28	109812.5
29	136236.5
30	163114.0
31	190014.5
32	220870.5
33	250059.0
34	277273.5
35	304256.0
36	333059.5
37	360202.5
38	379756.5
39	395549.5
40	413226.0
41	429555.0
42	440925.0
43	454676.5
44	468092.5
45	479116.0
46	494632.0
47	510689.5
48	515570.5
49	512341.0
50	506879.0
51	491730.0
52	468817.0
53	442022.5
54	415450.5
55	387038.5
56	357683.5
57	326966.5
58	292087.0
59	254005.0
60	211162.5
61	170735.5
62	135545.0
63	104942.0
64	78243.5
65	58035.5
66	43573.0
67	33207.0
68	25109.0
69	17945.0
70	12812.0
71	9281.0
72	6739.5
73	4620.5
74	3034.0
75	1962.0
76	1272.0
77	829.5
78	576.0
79	402.5
80	284.5
81	214.0
82	163.0
83	108.5
84	65.5
85	50.0
86	43.5
87	35.5
88	27.5
89	19.5
90	19.0
91	20.0
92	18.0
93	12.0
94	4.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	8.413383876456526E-6
7	0.0
8	0.0
9	0.0
10-11	0.0015438559413297722
12-13	3.491554308729458E-4
14-15	0.007361710891899459
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	179.0
20-21	698.0
22-23	1491.0
24-25	3123.0
26-27	4209.0
28-29	7171.0
30-31	16595.0
32-33	21456.0
34-35	30256.0
36-37	43523.0
38-39	51821.0
40-41	56913.0
42-43	68072.0
44-45	81303.0
46-47	79178.0
48-49	72841.0
50-51	63736.0
52-53	62234.0
54-55	72398.0
56-57	78380.0
58-59	80259.0
60-61	84654.0
62-63	84972.0
64-65	89518.0
66-67	94087.0
68-69	83534.0
70-71	83104.0
72-73	79771.0
74-75	82914.0
76-77	81181.0
78-79	89068.0
80-81	92399.0
82-83	90089.0
84-85	92304.0
86-87	97522.0
88-89	105781.0
90-91	113248.0
92-93	120847.0
94-95	149201.0
96-97	423043.0
98-99	757035.0
100-101	8095716.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.9401063731584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.8931982838138	69.4625151391202
2	8.556368257541624	13.67993977351587
3	2.3617604342890783	5.66398141034957
4	0.9434120582937494	3.016658411748906
5	0.46146093367707114	1.844461806260103
6	0.2531503909304388	1.2142121507631545
7	0.14424052290102224	0.8071421920819395
8	0.09560188201844902	0.6113939694423164
9	0.059793711762384856	0.4301924110857917
>10	0.22477555036440694	2.804948695302949
>50	0.004783565234524351	0.2544065324547797
>100	0.0014111280898100353	0.17831648928101843
>500	2.164057376575271E-5	0.012472937833123365
>1k	2.1640509874420347E-5	0.019358080760288825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.413383876456526E-6
2	0.0	8.413383876456526E-6	0.0	0.0	8.413383876456526E-6
3	0.0	8.413383876456526E-6	0.0	0.0	8.413383876456526E-6
4	8.413383876456526E-6	3.36535355058261E-5	0.0	8.413383876456526E-6	8.413383876456526E-6
5	8.413383876456526E-6	3.36535355058261E-5	0.0	8.413383876456526E-6	1.682676775291305E-5
6	1.9350782915850007E-4	3.36535355058261E-5	0.0	1.682676775291305E-5	1.682676775291305E-5
7	1.9350782915850007E-4	3.36535355058261E-5	0.0	1.682676775291305E-5	1.682676775291305E-5
8	1.9350782915850007E-4	3.36535355058261E-5	0.0	1.682676775291305E-5	5.048030325873915E-5
9	1.9350782915850007E-4	5.048030325873915E-5	0.0	3.36535355058261E-5	5.8893687135195673E-5
10-11	1.9350782915850007E-4	5.048030325873915E-5	0.0	4.2066919382282626E-5	5.8893687135195673E-5
12-13	1.9350782915850007E-4	5.8893687135195673E-5	0.0	5.048030325873915E-5	5.8893687135195673E-5
14-15	2.1033459691141313E-4	1.0516729845570655E-4	0.0	5.8893687135195673E-5	6.730707101165219E-5
16-17	3.786022744405436E-4	1.1778737427039135E-4	0.0	6.310037907342394E-5	7.572045488810872E-5
18-19	3.8701565831700014E-4	1.2199406620861961E-4	0.0	7.151376294988046E-5	8.413383876456525E-5
20-21	3.8701565831700014E-4	1.3040745008507614E-4	0.0	7.992714682633699E-5	1.009606065174783E-4
22-23	3.912223502552284E-4	1.4723421783798918E-4	0.0	8.834053070279352E-5	1.0516729845570656E-4
24-25	3.9963573413168493E-4	1.682676775291305E-4	0.0	1.1358068233216308E-4	1.0937399039393483E-4
26-27	4.038424260699132E-4	1.7668106140558703E-4	0.0	1.6406098559090224E-4	1.0937399039393483E-4
28-29	4.038424260699132E-4	1.7668106140558703E-4	0.0	3.5336212281117407E-4	1.1358068233216308E-4
30-31	4.0804911800814146E-4	2.019212130349566E-4	0.0	0.0012409741217773375	1.1778737427039135E-4
32-33	4.16462501884598E-4	2.1874798078786963E-4	0.0	0.0034873476167912297	1.1778737427039135E-4
34-35	4.2066919382282626E-4	2.902617437377501E-4	0.0	0.006019776163604644	1.346141420233044E-4
36-37	4.2066919382282626E-4	3.5336212281117407E-4	0.0	0.010659757371470416	1.4302752589976094E-4
38-39	4.2066919382282626E-4	3.8701565831700014E-4	0.0	0.024205305412565423	1.5144090977621744E-4
40-41	4.290825776992828E-4	4.290825776992828E-4	0.0	0.03908016810614056	1.5985429365267397E-4
42-43	4.290825776992828E-4	4.4170265351396753E-4	0.0	0.047724920039199634	1.7247436946735877E-4
44-45	4.3328926963751105E-4	4.837695728962502E-4	0.0	0.0585992186995197	1.8509444528204354E-4
46-47	4.374959615757393E-4	5.216298003403046E-4	0.0	0.07002459400374766	1.8509444528204354E-4
48-49	4.459093454521958E-4	5.216298003403046E-4	0.0	0.08123963471106421	1.8509444528204354E-4
50-51	5.048030325873915E-4	5.889368713519567E-4	0.0	0.09197511253742273	1.8509444528204354E-4
52-53	5.216298003403046E-4	6.141770229813263E-4	0.0	0.11729519131361865	1.9350782915850007E-4
54-55	5.216298003403046E-4	6.225904068577828E-4	0.0	0.14777688109802062	2.1033459691141313E-4
56-57	5.216298003403046E-4	6.225904068577828E-4	0.0	0.17924293679596803	2.481948243554675E-4
58-59	5.216298003403046E-4	6.267970987960111E-4	0.0	0.2084668256908398	2.818483598612936E-4
60-61	5.510766439079024E-4	6.394171746106959E-4	0.0	0.22026659657757006	2.860550517995219E-4
62-63	6.057636391048698E-4	6.478305584871524E-4	0.0	0.234401081490017	2.986751276142066E-4
64-65	6.057636391048698E-4	6.73070710116522E-4	0.0	0.23990343454521956	3.0708851149066315E-4
66-67	6.225904068577828E-4	6.814840939929785E-4	0.0	0.2443246677722975	3.3232866312003274E-4
68-69	6.225904068577828E-4	6.856907859312068E-4	0.0	0.24864073370091966	3.5756881474940233E-4
70-71	6.604506343018372E-4	6.898974778694351E-4	0.0	0.25274646503263043	3.7439558250231534E-4
72-73	7.487911650046307E-4	6.898974778694351E-4	0.0	0.2560276847444485	4.038424260699132E-4
74-75	7.487911650046307E-4	6.898974778694351E-4	0.0	0.2587620345042969	4.0804911800814146E-4
76-77	7.572045488810872E-4	7.151376294988047E-4	0.0	0.2613365299704926	4.1225580994636973E-4
78-79	7.656179327575437E-4	7.319643972517177E-4	0.0	0.2629603130586487	4.1225580994636973E-4
80-81	7.69824624695772E-4	7.319643972517177E-4	0.0	0.26356607669775356	4.2066919382282626E-4
82-83	7.782380085722285E-4	7.319643972517177E-4	0.0	0.2640919131900321	4.290825776992828E-4
84-85	7.992714682633699E-4	7.361710891899459E-4	0.0	0.2643232812466346	4.3328926963751105E-4
86-87	8.32925003769196E-4	7.572045488810872E-4	0.0	0.2643821749337698	4.417026535139676E-4
88	8.49751771522109E-4	7.908580843869133E-4	0.0	0.26439900170152275	4.4590934545219585E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14770	0.0	46.746204	1
GTATCAA	26285	0.0	36.11782	1
TCAACGC	30530	0.0	30.414007	4
ATCAACG	31155	0.0	29.776031	3
CAACGCA	31635	0.0	29.311514	5
TATCAAC	32280	0.0	29.033876	2
AACGCAG	32325	0.0	28.726091	6
CTTATAC	11395	0.0	28.506985	1
ACGCAGA	37735	0.0	24.561708	7
CGCAGAG	38030	0.0	24.34868	8
TATACAC	16335	0.0	23.523304	3
GTACATG	36475	0.0	22.36543	1
TACATGG	36425	0.0	21.967499	2
TTATACA	15770	0.0	21.395956	2
ACATGGG	37105	0.0	21.050629	3
GCAGAGT	44830	0.0	20.648825	9
GAGTACT	24530	0.0	19.265696	12-13
CATGGGG	28195	0.0	18.44297	4
CAGAGTA	43325	0.0	18.407495	10-11
AGAGTAC	39915	0.0	18.219526	10-11
>>END_MODULE