FastQCFastQC Report
Fri 27 May 2016
SRR2139102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3310949
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.048.870581
GTATCAA79150.037.888861
TCAACGC90350.032.4223984
ATCAACG91600.031.9325033
CAACGCA92750.031.6307725
AACGCAG96150.030.5122626
TATCAAC97150.030.3319452
ACGCAGA110850.026.4267737
CGCAGAG113000.025.885898
GCAGAGT131300.021.9493269
CTTATAC19850.021.89541
GTACATG102900.021.3721641
TACATGG104450.020.8469562
GAGTACT73600.020.22779712-13
ACATGGG105450.020.1958853
GTGGTAT24250.019.3564361
CAGAGTA128700.019.31923310-11
TAGCGCG1650.00557567419.26099294
TGGTATC22350.019.2517872
GTACTTT80100.018.72214914-15
Produced by FastQC (version 0.11.5)