##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139102_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3310949
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.563108039417095	33.0	33.0	33.0	33.0	33.0
2	32.56990941267896	33.0	33.0	33.0	33.0	33.0
3	32.562230949495145	33.0	33.0	33.0	33.0	33.0
4	36.45876544761034	37.0	37.0	37.0	37.0	37.0
5	36.48774656450462	37.0	37.0	37.0	37.0	37.0
6	36.48724761390164	37.0	37.0	37.0	37.0	37.0
7	36.46335899465682	37.0	37.0	37.0	37.0	37.0
8	36.44640675528376	37.0	37.0	37.0	37.0	37.0
9	36.483193791266494	37.0	37.0	37.0	37.0	37.0
10-11	36.43836495216326	37.0	37.0	37.0	37.0	37.0
12-13	36.370614588143766	37.0	37.0	37.0	37.0	37.0
14-15	38.39738380144182	40.0	37.0	40.0	37.0	40.0
16-17	38.35662796376508	40.0	37.0	40.0	37.0	40.0
18-19	38.36237963798295	40.0	37.0	40.0	37.0	40.0
20-21	38.35753085117405	40.0	37.0	40.0	37.0	40.0
22-23	38.352213493511634	40.0	37.0	40.0	37.0	40.0
24-25	38.34827848579632	40.0	37.0	40.0	37.0	40.0
26-27	38.19958367445108	40.0	37.0	40.0	37.0	40.0
28-29	38.12381131014406	40.0	37.0	40.0	33.0	40.0
30-31	38.08775540055825	40.0	37.0	40.0	33.0	40.0
32-33	38.00931300842011	40.0	37.0	40.0	33.0	40.0
34-35	37.97021652903659	40.0	37.0	40.0	33.0	40.0
36-37	37.932882469339646	40.0	37.0	40.0	33.0	40.0
38-39	37.884499137627174	40.0	37.0	40.0	33.0	40.0
40-41	37.854116334849934	40.0	37.0	40.0	33.0	40.0
42-43	37.75840510753166	40.0	37.0	40.0	33.0	40.0
44-45	37.70673023858042	40.0	37.0	40.0	33.0	40.0
46-47	37.672802827179325	40.0	37.0	40.0	33.0	40.0
48-49	37.64486321629255	40.0	37.0	40.0	33.0	40.0
50-51	37.57635237486794	40.0	37.0	40.0	33.0	40.0
52-53	37.4754363421883	40.0	37.0	40.0	33.0	40.0
54-55	37.26096619219965	40.0	37.0	40.0	33.0	40.0
56-57	37.07509685569092	37.0	37.0	40.0	33.0	40.0
58-59	37.00636861172505	37.0	37.0	40.0	33.0	40.0
60-61	36.865967069543515	37.0	37.0	40.0	33.0	40.0
62-63	36.680616915574234	37.0	37.0	40.0	33.0	40.0
64-65	36.53339978565207	37.0	37.0	40.0	33.0	40.0
66-67	36.3118564501463	37.0	37.0	40.0	33.0	40.0
68-69	36.09258838758261	37.0	37.0	40.0	33.0	40.0
70-71	35.82907237167892	37.0	35.0	37.0	33.0	40.0
72-73	35.61886318792444	37.0	33.0	37.0	33.0	40.0
74-75	35.444092921032635	37.0	33.0	37.0	33.0	40.0
76-77	33.645787209347105	35.0	33.0	37.0	27.0	37.0
78-79	34.900533411831475	37.0	33.0	37.0	33.0	37.0
80-81	34.978140164709345	37.0	33.0	37.0	33.0	37.0
82-83	34.8747309707348	37.0	33.0	37.0	33.0	37.0
84-85	34.725910116678236	37.0	33.0	37.0	33.0	37.0
86-87	34.60479540299986	37.0	33.0	37.0	33.0	37.0
88-89	34.53262303325065	37.0	33.0	37.0	33.0	37.0
90-91	34.45399232598483	37.0	33.0	37.0	33.0	37.0
92-93	34.388773063685576	37.0	33.0	37.0	30.0	37.0
94-95	34.37939682773532	37.0	33.0	37.0	30.0	37.0
96-97	34.39298695715418	37.0	33.0	37.0	33.0	37.0
98-99	34.40216649968886	37.0	33.0	37.0	33.0	37.0
100	34.406205130992646	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	26.0
21	157.0
22	632.0
23	1683.0
24	3788.0
25	7441.0
26	12935.0
27	20273.0
28	29353.0
29	40937.0
30	54729.0
31	72239.0
32	94253.0
33	125371.0
34	170730.0
35	244126.0
36	415263.0
37	950815.0
38	1043791.0
39	22400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.589767767489015	19.936882144666075	14.357545223438958	24.115804864405945
2	14.663499800208339	20.56398331716979	41.90408852567648	22.868428356945394
3	18.503244840074554	25.692573337734892	29.997834457734022	25.806347364456535
4	12.696027634373106	17.916404027969023	38.200044760580724	31.187523577077148
5	13.76599277125682	37.43793697818963	35.13340132995102	13.662668920602522
6	30.571636884662638	37.262802073605506	18.558249782237596	13.60731125949426
7	27.08066478825255	32.29723562640198	22.485849223289154	18.136250362056316
8	24.545137964976206	36.98818677062075	20.642178420748856	17.82449684365419
9	25.627093621798462	17.04233438811652	20.747918497083464	36.58265349300155
10-11	24.659068411030685	26.60867926357599	28.292410065385713	20.439842260007616
12-13	25.465309770836946	23.973361639498396	28.467841227542817	22.093487362121838
14-15	22.501988996935403	25.41330963653158	26.06105510775017	26.02364625878285
16-17	22.18933302808349	28.58962792842777	26.769605934733516	22.45143310875522
18-19	22.24652206965435	27.626052228530252	28.413092439660048	21.714333262155353
20-21	22.94024717713455	26.76082032351369	28.437859658708476	21.86107284064328
22-23	22.704015595884393	26.774404891897795	28.24949246620796	22.272087046009855
24-25	22.50590704437511	26.94586546168299	28.332045832803377	22.21618166113852
26-27	22.44347955375955	27.107859373092364	28.27388255545066	22.17477851769743
28-29	22.30427960747087	27.08027740659899	28.342030001995294	22.273412983934847
30-31	22.639498369058046	27.002720026648124	28.158984733148912	22.19879687114492
32-33	22.443435473007877	26.994168655439854	28.238756399951402	22.323639471600863
34-35	22.484354806105223	27.173511862665883	28.241798300381653	22.10033503084724
36-37	22.558055918159106	27.060367138567777	28.077063707030465	22.304513236242652
38-39	22.3884355781936	27.130370214707796	28.16093340802422	22.320260799074386
40-41	22.570389537086317	27.01906042986047	28.05283605760308	22.35771397545013
42-43	22.57751628825028	27.096287137449544	27.85832956096054	22.467867013339635
44-45	22.52730264025021	27.222783031814185	27.72323193792536	22.52668239001025
46-47	22.716701648557382	27.17018624772925	27.41051043164041	22.70260167207296
48-49	22.61127753171004	27.33686274953658	27.358430356123314	22.693429362630066
50-51	22.585301671242235	27.485846311501334	27.284800678111914	22.644051339144518
52-53	22.769101948293144	27.37969773216439	27.286391841490655	22.56480847805181
54-55	22.677344611925008	27.30355336086136	27.224938404157957	22.794163623055674
56-57	22.759376341857056	27.48907040327653	27.107653067465115	22.643900187401304
58-59	22.662661118612107	27.546687151325884	27.20984715413221	22.580804575929804
60-61	22.782016581287813	27.57524648973716	27.147098600952823	22.495638328022206
62-63	22.742638018110654	27.705659074033633	27.062777038105786	22.488925869749924
64-65	22.693599640013986	27.59432131664321	27.048599601122525	22.663479442220275
66-67	22.784886982302112	27.516733184623572	26.929689349159307	22.76869048391501
68-69	22.69515529554296	27.48407551752992	27.07035084719575	22.75041833973137
70-71	22.696631993603408	27.328890652915394	27.114608884931503	22.859868468549692
72-73	22.761949516167135	27.357938953477944	27.022657784824585	22.85745374553034
74-75	22.63787609790712	27.53407576968624	26.987000793438614	22.841047338968025
76-77	22.80238841736285	27.412826688408582	26.963584028300584	22.82120086592798
78-79	22.750164413182315	27.43729636477991	26.944931821467062	22.867607400570712
80-81	22.732419316617055	27.479144674736034	26.979150148806426	22.80928585984049
82-83	22.760197566858807	27.426168609039138	27.04664634445174	22.76698747965031
84-85	22.82026331882442	27.41251483866326	27.01843591496097	22.748785927551353
86-87	22.753296989821784	27.404925112320626	27.070305520814724	22.77147237704287
88-89	22.69276169236431	27.394805138575013	27.044060811338888	22.868372357721785
90-91	22.76247861312866	27.4713573658055	27.001143107396423	22.765020913669414
92-93	22.81963235044075	27.40252315446726	27.032160647109514	22.74568384798247
94-95	22.6429070747683	27.42818775127646	27.12707017145974	22.80183500249551
96-97	22.735883465444104	27.55339814121759	27.12090320340294	22.58981518993537
98-99	23.91181552610966	29.474601948011557	27.28135473144998	19.3322277944288
100	22.755903319593944	30.498529058876095	21.07986567304671	25.665701948483253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	545.0
1	485.5
2	666.5
3	971.0
4	1319.5
5	2190.5
6	2904.0
7	3339.5
8	3607.5
9	3536.0
10	3279.5
11	3036.0
12	3362.0
13	4516.5
14	7243.0
15	11116.5
16	14433.0
17	16589.5
18	17337.5
19	16548.5
20	14753.5
21	12664.0
22	11230.5
23	11062.0
24	12176.0
25	14691.5
26	18728.0
27	23990.0
28	29710.5
29	36446.5
30	43863.0
31	51198.0
32	59148.0
33	67369.0
34	75090.0
35	81973.5
36	89786.0
37	97891.0
38	104696.0
39	110135.5
40	115431.0
41	120697.0
42	124704.5
43	128216.0
44	131427.5
45	135166.5
46	138548.0
47	141243.0
48	142192.0
49	140664.5
50	138982.0
51	135931.5
52	130212.0
53	123057.5
54	116072.5
55	108455.5
56	100018.0
57	90434.5
58	80131.0
59	69487.0
60	57947.5
61	47523.5
62	38131.0
63	29477.0
64	22021.0
65	16399.0
66	12379.0
67	9404.0
68	7129.0
69	5175.5
70	3703.5
71	2640.0
72	1793.5
73	1165.5
74	759.5
75	504.0
76	332.5
77	214.0
78	138.0
79	93.0
80	68.5
81	55.5
82	43.5
83	23.5
84	13.5
85	12.0
86	9.5
87	8.5
88	6.5
89	4.0
90	3.0
91	2.5
92	2.0
93	1.5
94	0.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	3.0202821003887403E-5
7	0.0
8	0.0
9	0.0
10-11	0.0017366622077235256
12-13	2.869267995369304E-4
14-15	0.00709766293591354
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	47.0
20-21	202.0
22-23	381.0
24-25	982.0
26-27	1162.0
28-29	1884.0
30-31	4383.0
32-33	5618.0
34-35	7679.0
36-37	11300.0
38-39	13741.0
40-41	15275.0
42-43	18413.0
44-45	22229.0
46-47	21978.0
48-49	19505.0
50-51	17498.0
52-53	17423.0
54-55	19376.0
56-57	20721.0
58-59	21285.0
60-61	21634.0
62-63	21461.0
64-65	22938.0
66-67	24025.0
68-69	23623.0
70-71	23460.0
72-73	22573.0
74-75	23471.0
76-77	23299.0
78-79	25377.0
80-81	25869.0
82-83	25718.0
84-85	26051.0
86-87	27576.0
88-89	30312.0
90-91	32712.0
92-93	34427.0
94-95	41992.0
96-97	118852.0
98-99	211660.0
100-101	2262837.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.65823655402957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.34805840505858	82.79764065786081
2	5.846224840831569	10.483244194546415
3	1.1005176288713594	2.9601140970368425
4	0.3512568146538718	1.2597226631780705
5	0.13822311328187278	0.6196420293930288
6	0.07304793430417855	0.3929609384176352
7	0.04460697661847313	0.27995680031333914
8	0.02356969500538142	0.16905738322390534
9	0.020934663378980287	0.1689268501270435
>10	0.052195187849353564	0.721105775336406
>50	8.193796523617603E-4	0.052113342645202225
>100	5.453604940153156E-4	0.09551526792131487
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.0202821003887406E-5	0.0	0.0	0.0	0.0
7	3.0202821003887406E-5	0.0	0.0	3.0202821003887406E-5	0.0
8	3.0202821003887406E-5	0.0	0.0	3.0202821003887406E-5	0.0
9	3.0202821003887406E-5	6.040564200777481E-5	0.0	3.0202821003887406E-5	0.0
10-11	3.0202821003887406E-5	6.040564200777481E-5	0.0	3.0202821003887406E-5	0.0
12-13	3.0202821003887406E-5	6.040564200777481E-5	0.0	3.0202821003887406E-5	0.0
14-15	3.0202821003887406E-5	2.5672397853304294E-4	0.0	6.040564200777481E-5	0.0
16-17	6.040564200777481E-5	3.0202821003887407E-4	0.0	9.060846301166221E-5	0.0
18-19	6.040564200777481E-5	3.1712962054081777E-4	0.0	1.0570987351360592E-4	0.0
20-21	6.040564200777481E-5	3.6243385204664884E-4	0.0	1.2081128401554962E-4	0.0
22-23	6.040564200777481E-5	4.2283949405442367E-4	0.0	1.2081128401554962E-4	0.0
24-25	6.040564200777481E-5	4.5304231505831105E-4	0.0	1.2081128401554962E-4	0.0
26-27	6.040564200777481E-5	4.5304231505831105E-4	0.0	1.3591269451749334E-4	0.0
28-29	6.040564200777481E-5	4.5304231505831105E-4	0.0	1.6611551552138073E-4	0.0
30-31	7.550705250971852E-5	5.738535990738607E-4	0.0	3.6243385204664884E-4	0.0
32-33	9.060846301166221E-5	5.889550095758044E-4	0.0	0.0011024029666418904	0.0
34-35	9.060846301166221E-5	6.795634725874667E-4	0.0	0.0023709214488051612	0.0
36-37	9.060846301166221E-5	7.852733461010726E-4	0.0	0.004062279425022856	0.0
38-39	9.060846301166221E-5	8.305775776069036E-4	0.0	0.007173169988423259	0.0
40-41	9.060846301166221E-5	0.0010268959141321718	0.0	0.010465277477846986	0.0
42-43	9.060846301166221E-5	0.0010268959141321718	0.0	0.013832892019780432	0.0
44-45	1.0570987351360592E-4	0.0011477071981477213	0.0	0.016898478351675003	0.0
46-47	1.2081128401554962E-4	0.001268518482163271	0.0	0.020145281609592898	0.0
48-49	1.2081128401554962E-4	0.0012836198926652147	0.0	0.023724315898553554	0.0
50-51	1.2081128401554962E-4	0.0013591269451749333	0.0	0.028118826354619174	0.0
52-53	1.2081128401554962E-4	0.001374228355676877	0.0	0.03195458462211287	0.0
54-55	1.2081128401554962E-4	0.0013893297661788206	0.0	0.03585074853161435	0.0
56-57	1.2081128401554962E-4	0.0013893297661788206	0.0	0.03985262231462943	0.0
58-59	1.2081128401554962E-4	0.0013893297661788206	0.0	0.044398146875714486	0.0
60-61	1.2081128401554962E-4	0.001419532587182708	0.0	0.048671846047764554	0.0
62-63	1.2081128401554962E-4	0.001419532587182708	0.0	0.05302105227232434	3.0202821003887406E-5
64-65	1.2081128401554962E-4	0.0014799382291904829	0.0	0.05774779375943272	3.0202821003887406E-5
66-67	1.2081128401554962E-4	0.0014799382291904829	0.0	0.062459433836039156	3.0202821003887406E-5
68-69	1.2081128401554962E-4	0.001525242460696314	0.0	0.06729188519666114	3.0202821003887406E-5
70-71	1.2081128401554962E-4	0.0015705466922021451	0.0	0.07144477308469566	3.0202821003887406E-5
72-73	1.2081128401554962E-4	0.0015705466922021451	0.0	0.074570765058598	3.0202821003887406E-5
74-75	1.2081128401554962E-4	0.0015705466922021451	0.0	0.07703229497041483	3.0202821003887406E-5
76-77	1.2081128401554962E-4	0.0015705466922021451	0.0	0.07982605591327441	3.0202821003887406E-5
78-79	1.2081128401554962E-4	0.0015705466922021451	0.0	0.08195535479404847	3.0202821003887406E-5
80-81	1.2081128401554962E-4	0.0015705466922021451	0.0	0.08261981685613401	3.0202821003887406E-5
82-83	1.2081128401554962E-4	0.0015705466922021451	0.0	0.08281613519265926	3.0202821003887406E-5
84-85	1.2081128401554962E-4	0.0016007495132060324	0.0	0.08305775776069035	3.0202821003887406E-5
86-87	1.2081128401554962E-4	0.0016007495132060324	0.0	0.08314836622370203	3.0202821003887406E-5
88	1.2081128401554962E-4	0.0016611551552138072	0.0	0.08314836622370203	3.0202821003887406E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4420	0.0	48.87058	1
GTATCAA	7915	0.0	37.88886	1
TCAACGC	9035	0.0	32.422398	4
ATCAACG	9160	0.0	31.932503	3
CAACGCA	9275	0.0	31.630772	5
AACGCAG	9615	0.0	30.512262	6
TATCAAC	9715	0.0	30.331945	2
ACGCAGA	11085	0.0	26.426773	7
CGCAGAG	11300	0.0	25.88589	8
GCAGAGT	13130	0.0	21.949326	9
CTTATAC	1985	0.0	21.8954	1
GTACATG	10290	0.0	21.372164	1
TACATGG	10445	0.0	20.846956	2
GAGTACT	7360	0.0	20.227797	12-13
ACATGGG	10545	0.0	20.195885	3
GTGGTAT	2425	0.0	19.356436	1
CAGAGTA	12870	0.0	19.319233	10-11
TAGCGCG	165	0.005575674	19.260992	94
TGGTATC	2235	0.0	19.251787	2
GTACTTT	8010	0.0	18.722149	14-15
>>END_MODULE