FastQCFastQC Report
Fri 27 May 2016
SRR2139101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8604804
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110300.049.8581
GTATCAA197450.038.1892281
TCAACGC239150.030.7510664
ATCAACG240400.030.5911733
CAACGCA245650.029.9211335
TATCAAC249850.029.711652
AACGCAG253350.028.994646
CTTATAC59400.025.8994581
ACGCAGA289600.025.3353757
CGCAGAG294050.024.9523988
GTACATG267150.021.5909441
TATACAC92000.021.0085373
GCAGAGT349450.020.988329
TACATGG271650.020.722792
GAGTACT193950.020.0579612-13
ACATGGG273850.019.8600223
GTGGTAT58800.019.4569781
TTATACA88050.019.1948182
CAGAGTA338700.018.67889610-11
TGGTATC55850.018.622452
Produced by FastQC (version 0.11.5)