##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139101_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8604804
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56575629148555	33.0	33.0	33.0	33.0	33.0
2	32.57543390877933	33.0	33.0	33.0	33.0	33.0
3	32.569059911184496	33.0	33.0	33.0	33.0	33.0
4	36.46808933707264	37.0	37.0	37.0	37.0	37.0
5	36.496776916708384	37.0	37.0	37.0	37.0	37.0
6	36.4948357917275	37.0	37.0	37.0	37.0	37.0
7	36.4715127735623	37.0	37.0	37.0	37.0	37.0
8	36.45385263859583	37.0	37.0	37.0	37.0	37.0
9	36.490277640257695	37.0	37.0	37.0	37.0	37.0
10-11	36.4468452738726	37.0	37.0	37.0	37.0	37.0
12-13	36.375022952295026	37.0	37.0	37.0	37.0	37.0
14-15	38.40979463332343	40.0	37.0	40.0	37.0	40.0
16-17	38.3684724254033	40.0	37.0	40.0	37.0	40.0
18-19	38.375548007833764	40.0	37.0	40.0	37.0	40.0
20-21	38.36946935355108	40.0	37.0	40.0	37.0	40.0
22-23	38.363705423715885	40.0	37.0	40.0	37.0	40.0
24-25	38.36123687833668	40.0	37.0	40.0	37.0	40.0
26-27	38.20967702897883	40.0	37.0	40.0	37.0	40.0
28-29	38.13228043777006	40.0	37.0	40.0	35.0	40.0
30-31	38.09353025407543	40.0	37.0	40.0	33.0	40.0
32-33	38.01661528639052	40.0	37.0	40.0	33.0	40.0
34-35	37.981689182905086	40.0	37.0	40.0	33.0	40.0
36-37	37.941000512233764	40.0	37.0	40.0	33.0	40.0
38-39	37.889090174848036	40.0	37.0	40.0	33.0	40.0
40-41	37.855107601372765	40.0	37.0	40.0	33.0	40.0
42-43	37.75886740563449	40.0	37.0	40.0	33.0	40.0
44-45	37.709517016967794	40.0	37.0	40.0	33.0	40.0
46-47	37.67087458136122	40.0	37.0	40.0	33.0	40.0
48-49	37.64334622809612	40.0	37.0	40.0	33.0	40.0
50-51	37.57278722485658	40.0	37.0	40.0	33.0	40.0
52-53	37.471943507658025	40.0	37.0	40.0	33.0	40.0
54-55	37.253481230969854	40.0	37.0	40.0	33.0	40.0
56-57	37.061440839708396	37.0	37.0	40.0	33.0	40.0
58-59	37.00758730572748	37.0	37.0	40.0	33.0	40.0
60-61	36.869480183006516	37.0	37.0	40.0	33.0	40.0
62-63	36.68393264999585	37.0	37.0	40.0	33.0	40.0
64-65	36.53913840674192	37.0	37.0	40.0	33.0	40.0
66-67	36.31834215645897	37.0	37.0	40.0	33.0	40.0
68-69	36.09927513713579	37.0	37.0	40.0	33.0	40.0
70-71	35.839857672453334	37.0	35.0	37.0	33.0	40.0
72-73	35.63095903830444	37.0	33.0	37.0	33.0	40.0
74-75	35.45807686495097	37.0	33.0	37.0	33.0	40.0
76-77	33.656967761721575	35.0	33.0	37.0	27.0	37.0
78-79	34.91112383249796	37.0	33.0	37.0	33.0	37.0
80-81	34.98925630119528	37.0	33.0	37.0	33.0	37.0
82-83	34.88395220508804	37.0	33.0	37.0	33.0	37.0
84-85	34.73588723959206	37.0	33.0	37.0	33.0	37.0
86-87	34.615856249122025	37.0	33.0	37.0	33.0	37.0
88-89	34.54099915808097	37.0	33.0	37.0	33.0	37.0
90-91	34.46154567966383	37.0	33.0	37.0	33.0	37.0
92-93	34.39627442214095	37.0	33.0	37.0	30.0	37.0
94-95	34.38746704349609	37.0	33.0	37.0	33.0	37.0
96-97	34.39976137308501	37.0	33.0	37.0	33.0	37.0
98-99	34.40585763428872	37.0	33.0	37.0	33.0	37.0
100	34.41523996632051	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	20.0
20	91.0
21	430.0
22	1632.0
23	4450.0
24	9922.0
25	19537.0
26	33282.0
27	52485.0
28	76499.0
29	106143.0
30	141793.0
31	186159.0
32	244872.0
33	323318.0
34	440665.0
35	632906.0
36	1080756.0
37	2470813.0
38	2719033.0
39	59996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6471310677152	19.916827855695495	14.429672076203016	24.006369000386297
2	14.66286739360943	20.54025867410809	41.83927954663465	22.95759438564783
3	18.407740606293878	25.698842181646437	30.097652427643908	25.795764784415777
4	12.633175607486235	17.873399556805712	38.19388564806357	31.29953918764448
5	13.69201436778804	37.51619444208142	35.169877198829866	13.621913991300675
6	30.563647934339933	37.29521323205038	18.48969482628541	13.651444007324281
7	27.011399678598142	32.374671172056914	22.479477742898037	18.13445140644691
8	24.633355971850143	36.84437205077536	20.660075464821745	17.862196512552757
9	25.625766722867834	16.898455792833865	20.741448614053265	36.734328870245044
10-11	24.653957573884735	26.554475816747054	28.329898951183736	20.461667658184474
12-13	25.43840147527236	23.957440419190068	28.508647050292023	22.09551105524555
14-15	22.464835090503747	25.407046626439676	26.083253314031076	26.0448649690255
16-17	22.168883800258556	28.570534552559245	26.753061429406177	22.507520217776023
18-19	22.228827059860983	27.60815934912637	28.469974446832257	21.69303914418039
20-21	22.967977211235258	26.765287275130216	28.422112102713537	21.844623410920985
22-23	22.68117838277548	26.79347827034449	28.278337918149056	22.247005428730972
24-25	22.522024284094883	26.93532644078304	28.30654588070661	22.236103394415462
26-27	22.444438320119325	27.045949997366154	28.29703333427524	22.21257834823928
28-29	22.33283106819609	27.050871867713777	28.330726033417065	22.285571030673072
30-31	22.560681248510864	26.95288439781712	28.24909769883734	22.23733665483468
32-33	22.273420555891942	26.97934728479181	28.374446493077382	22.37278566623887
34-35	22.4261190188148	27.066186992115444	28.23539809165636	22.272295897413404
36-37	22.455077701574897	27.095223375662865	28.151326538747284	22.298372384014957
38-39	22.356849495967133	27.12118083651231	28.199235374848808	22.322734292671747
40-41	22.587864005999243	26.97000595942522	28.108972819479277	22.333157215096257
42-43	22.510395120577343	27.064682076438658	27.970868544145127	22.454054258838873
44-45	22.53319748785458	27.194804806778727	27.78146007995363	22.490537625413065
46-47	22.722335760658837	27.19342334759038	27.404154993128305	22.680085898622483
48-49	22.562113290412785	27.306326794054236	27.428957221264476	22.702602694268506
50-51	22.558496817700636	27.460871100570593	27.378677181858986	22.601954899869785
52-53	22.74048540493652	27.362077552175545	27.36787026675696	22.52956677613097
54-55	22.694131769825113	27.24991459415773	27.30734361841497	22.748610017602182
56-57	22.73900776165457	27.48879033016895	27.136978293395593	22.63522361478089
58-59	22.622785794410866	27.538091724563568	27.281896880793038	22.557225600232524
60-61	22.762051249848213	27.532213521993665	27.191803787001557	22.513931441156565
62-63	22.6898771878619	27.673742964536206	27.111813990681956	22.524565856919935
64-65	22.68865171445964	27.585110158512705	27.07521299768867	22.651025129338986
66-67	22.76537279171026	27.535249615932088	26.990909721069166	22.708467871288484
68-69	22.68598655564147	27.4913091184403	27.077643049303568	22.745061276614667
70-71	22.71330915538806	27.324237046625843	27.12444065727415	22.838013140711947
72-73	22.761211482824702	27.314809431746472	27.069417856980525	22.854561228448297
74-75	22.600753877410586	27.47902272546121	27.075528443000827	22.84469495412737
76-77	22.807848725011514	27.378025205381746	26.995683406677447	22.818442662929293
78-79	22.71170039787214	27.44114795247793	26.96113975308816	22.886011896561772
80-81	22.723494096850224	27.481645882605378	26.98652823392714	22.80833178661726
82-83	22.720706360752427	27.43165590950608	27.062720767040876	22.78491696270062
84-85	22.83984579258196	27.375832592620743	27.016358177359134	22.767963437438166
86-87	22.718235634462367	27.427921631036384	27.06358248603109	22.79026024847016
88-89	22.72306492931106	27.397388854492682	27.05361453653161	22.825931679664645
90-91	22.74599542334096	27.44069391716556	27.037807167753265	22.775503491740217
92-93	22.772752176542934	27.367860707649143	27.081866306285953	22.777520809521974
94-95	22.63143513383036	27.479421296649303	27.11237766286576	22.776765906654575
96-97	22.735691007192624	27.56274200164377	27.131853238633717	22.56971375252989
98-99	23.86490294082246	29.466840764626234	27.32675529657596	19.341500997975345
100	22.776759241944973	30.428141717742545	21.15772028320576	25.63737875710672
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1708.0
1	1524.0
2	1993.5
3	2805.5
4	3738.5
5	6079.5
6	8137.5
7	9437.5
8	10141.5
9	10003.0
10	9280.5
11	8414.5
12	9171.5
13	12431.5
14	19898.5
15	30266.5
16	39281.0
17	45153.5
18	46767.5
19	44175.0
20	39201.0
21	34018.5
22	30598.0
23	30424.5
24	33937.0
25	41371.5
26	52354.0
27	65149.5
28	80041.5
29	97912.5
30	116903.0
31	136047.5
32	156424.5
33	176771.5
34	196672.5
35	215343.5
36	235029.5
37	254508.5
38	271050.0
39	285109.5
40	298595.5
41	311090.5
42	320016.0
43	328989.5
44	336550.0
45	344265.0
46	350463.5
47	354331.5
48	361744.5
49	361979.0
50	354362.0
51	348042.5
52	338774.0
53	323662.0
54	306096.0
55	286553.0
56	264892.5
57	240484.5
58	213856.0
59	186037.5
60	156111.0
61	127974.0
62	102901.5
63	79834.5
64	59586.0
65	43940.0
66	32839.0
67	25021.5
68	18849.0
69	13708.0
70	9809.0
71	6896.5
72	4677.0
73	3102.0
74	2099.5
75	1408.0
76	948.0
77	622.0
78	419.0
79	307.5
80	232.5
81	169.5
82	120.5
83	84.5
84	55.5
85	42.0
86	34.5
87	25.0
88	18.0
89	13.0
90	9.0
91	5.5
92	4.0
93	2.0
94	0.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0017548336952242026
12-13	2.3242830400320566E-4
14-15	0.007321491576100978
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	130.0
20-21	516.0
22-23	1031.0
24-25	2728.0
26-27	3226.0
28-29	5152.0
30-31	12051.0
32-33	15341.0
34-35	21646.0
36-37	31410.0
38-39	38511.0
40-41	41175.0
42-43	50109.0
44-45	60140.0
46-47	59715.0
48-49	54442.0
50-51	47909.0
52-53	47288.0
54-55	53052.0
56-57	56140.0
58-59	58001.0
60-61	57955.0
62-63	56552.0
64-65	59532.0
66-67	64726.0
68-69	61402.0
70-71	61809.0
72-73	59337.0
74-75	61435.0
76-77	60253.0
78-79	65681.0
80-81	68478.0
82-83	67101.0
84-85	67589.0
86-87	71955.0
88-89	78083.0
90-91	83377.0
92-93	88326.0
94-95	107640.0
96-97	305745.0
98-99	547669.0
100-101	5850446.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.73394220813653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.01054753778452	74.53204043443438
2	7.598642125463773	12.725285211877905
3	1.8389685852872664	4.619522677290677
4	0.6927530886897524	2.320277883714232
5	0.3272603591016405	1.3701399998015387
6	0.1813197621630054	0.9109571091690092
7	0.09682326948953475	0.5675175836287673
8	0.061605467104395864	0.41267748977797913
9	0.04419464412976441	0.3330532599723768
>10	0.14376168360497288	1.8840601122347347
>50	0.0033806047548567662	0.19331548563908996
>100	6.943055375379552E-4	0.10303164082420677
>500	4.856688900076531E-5	0.028121111635124228
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1621415200160282E-5
2	0.0	1.1621415200160282E-5	0.0	1.1621415200160282E-5	1.1621415200160282E-5
3	0.0	1.1621415200160282E-5	0.0	2.3242830400320564E-5	1.1621415200160282E-5
4	0.0	1.1621415200160282E-5	0.0	3.486424560048085E-5	2.3242830400320564E-5
5	0.0	2.3242830400320564E-5	0.0	3.486424560048085E-5	2.3242830400320564E-5
6	1.1621415200160282E-5	2.3242830400320564E-5	0.0	4.648566080064113E-5	4.648566080064113E-5
7	1.1621415200160282E-5	2.3242830400320564E-5	0.0	5.8107076000801414E-5	4.648566080064113E-5
8	1.1621415200160282E-5	2.3242830400320564E-5	0.0	5.8107076000801414E-5	4.648566080064113E-5
9	1.1621415200160282E-5	1.1621415200160283E-4	0.0	6.97284912009617E-5	8.134990640112197E-5
10-11	1.1621415200160282E-5	1.1621415200160283E-4	0.0	8.134990640112197E-5	9.297132160128225E-5
12-13	2.3242830400320564E-5	1.278355672017631E-4	0.0	8.134990640112197E-5	9.87820292013624E-5
14-15	2.3242830400320564E-5	1.9175335080264466E-4	0.0	9.87820292013624E-5	1.5107839760208366E-4
16-17	2.3242830400320564E-5	1.975640584027248E-4	0.0	1.278355672017631E-4	1.859426432025645E-4
18-19	2.3242830400320564E-5	2.0337476600280495E-4	0.0	1.4526769000200354E-4	1.859426432025645E-4
20-21	2.9053538000400707E-5	2.2080688880304536E-4	0.0	1.5688910520216383E-4	1.859426432025645E-4
22-23	3.486424560048085E-5	2.2080688880304536E-4	0.0	1.6269981280224395E-4	1.975640584027248E-4
24-25	4.067495320056099E-5	2.847246724039269E-4	0.0	1.9175335080264466E-4	2.1499618120296524E-4
26-27	5.229636840072127E-5	2.9053538000400707E-4	0.0	2.3823901160328578E-4	2.2080688880304536E-4
28-29	5.8107076000801414E-5	2.9634608760408724E-4	0.0	4.648566080064113E-4	2.2080688880304536E-4
30-31	5.8107076000801414E-5	3.253996256044879E-4	0.0	0.0010807916136149063	2.2080688880304536E-4
32-33	6.391778360088156E-5	3.253996256044879E-4	0.0	0.002388200823632938	2.556711344035262E-4
34-35	7.553919880104183E-5	3.7769599400520914E-4	0.0	0.004189520179657782	2.672925496036865E-4
36-37	8.134990640112197E-5	4.2999236240593043E-4	0.0	0.007048388318897212	2.963460876040872E-4
38-39	8.134990640112197E-5	4.416137776060907E-4	0.0	0.011197233545354433	3.3121033320456807E-4
40-41	9.297132160128225E-5	5.694493448078538E-4	0.0	0.015520399999814057	3.370210408046482E-4
42-43	9.297132160128225E-5	5.75260052407934E-4	0.0	0.01934965630826687	3.428317484047283E-4
44-45	9.297132160128225E-5	6.449885436088957E-4	0.0	0.023568230025925053	3.486424560048085E-4
46-47	9.297132160128225E-5	7.147170348098574E-4	0.0	0.027670589591581633	3.602638712049687E-4
48-49	9.297132160128225E-5	7.205277424099376E-4	0.0	0.032173887981643744	3.71885286405129E-4
50-51	1.0459273680144255E-4	7.553919880104183E-4	0.0	0.03629367967010056	3.7769599400520914E-4
52-53	1.1040344440152268E-4	7.670134032105786E-4	0.0	0.03989631838215025	3.951281168054496E-4
54-55	1.1621415200160283E-4	7.78634818410739E-4	0.0	0.043539632047400495	4.067495320056099E-4
56-57	1.1621415200160283E-4	8.018776488110595E-4	0.0	0.04739794189385371	4.067495320056099E-4
58-59	1.1621415200160283E-4	8.076883564111396E-4	0.0	0.0516165156115119	4.067495320056099E-4
60-61	1.278355672017631E-4	8.367418944115403E-4	0.0	0.055893196405170875	4.067495320056099E-4
62-63	1.5107839760208368E-4	8.483633096117006E-4	0.0	0.05983866686562529	4.067495320056099E-4
64-65	1.5107839760208368E-4	9.06470385612502E-4	0.0	0.06390035147808132	4.067495320056099E-4
66-67	1.5107839760208368E-4	9.180918008126623E-4	0.0	0.06761339363453253	4.067495320056099E-4
68-69	1.5107839760208368E-4	9.239025084127425E-4	0.0	0.07171575320018911	4.241816548058503E-4
70-71	1.5107839760208368E-4	9.297132160128226E-4	0.0	0.07553338809344176	4.416137776060907E-4
72-73	1.6269981280224395E-4	9.413346312129829E-4	0.0	0.07863630595188456	4.648566080064113E-4
74-75	1.6269981280224395E-4	9.645774616133035E-4	0.0	0.08111747809711878	4.706673156064915E-4
76-77	1.6269981280224395E-4	9.87820292013624E-4	0.0	0.08383107854635619	4.93910146006812E-4
78-79	1.6269981280224395E-4	9.93630999613704E-4	0.0	0.08553942658077976	5.113422688070524E-4
80-81	1.859426432025645E-4	9.994417072137843E-4	0.0	0.08618441512438865	5.171529764071326E-4
82-83	1.859426432025645E-4	9.994417072137843E-4	0.0	0.08656211111839386	5.229636840072127E-4
84-85	1.859426432025645E-4	9.994417072137843E-4	0.0	0.08682940366799755	5.287743916072929E-4
86-87	1.859426432025645E-4	0.0010226845376141048	0.0	0.08691075357439867	5.34585099207373E-4
88	2.091854736028851E-4	0.0010575487832145858	0.0	0.08691656428199876	5.34585099207373E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11030	0.0	49.858	1
GTATCAA	19745	0.0	38.189228	1
TCAACGC	23915	0.0	30.751066	4
ATCAACG	24040	0.0	30.591173	3
CAACGCA	24565	0.0	29.921133	5
TATCAAC	24985	0.0	29.71165	2
AACGCAG	25335	0.0	28.99464	6
CTTATAC	5940	0.0	25.899458	1
ACGCAGA	28960	0.0	25.335375	7
CGCAGAG	29405	0.0	24.952398	8
GTACATG	26715	0.0	21.590944	1
TATACAC	9200	0.0	21.008537	3
GCAGAGT	34945	0.0	20.98832	9
TACATGG	27165	0.0	20.72279	2
GAGTACT	19395	0.0	20.05796	12-13
ACATGGG	27385	0.0	19.860022	3
GTGGTAT	5880	0.0	19.456978	1
TTATACA	8805	0.0	19.194818	2
CAGAGTA	33870	0.0	18.678896	10-11
TGGTATC	5585	0.0	18.62245	2
>>END_MODULE