FastQCFastQC Report
Fri 27 May 2016
SRR2139100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9684730
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128250.048.3574831
GTATCAA219250.035.893141
TCAACGC263250.029.3329834
ATCAACG267450.028.888583
CAACGCA272700.028.3648575
TATCAAC278700.027.8315472
AACGCAG280450.027.6274786
ACGCAGA323800.023.9153257
CGCAGAG329550.023.4720578
GTACATG300250.022.2467291
TACATGG300350.021.5163272
ACATGGG302950.020.7869073
GCAGAGT391600.019.6662569
GAGTACT216600.019.39164712-13
CATGGGG222200.018.841954
GTGGTAT66400.018.7718331
CAGAGTA375750.018.3167710-11
AGAGTAC349050.018.08768810-11
TGGTATC63750.017.6446252
GTACTTT237900.017.60103614-15
Produced by FastQC (version 0.11.5)